New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP000730 = 26352058 (1.490)4 (0.110) 4/268 5.4 7.1% intergenic (+19/‑85) pflA/USA300HOU_0235 [formate‑C‑acetyltransferase]‑activating enzyme/hypothetical protein
?CP000730 = 263562 49 (1.310)intergenic (+61/‑43) pflA/USA300HOU_0235 [formate‑C‑acetyltransferase]‑activating enzyme/hypothetical protein
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GGAAAACATTGGGCATTGCATATGAATTAGAAGATGTCGAAGCGCCCGATGATGAAGCTGTTAAAGCAGCCTACCGTTATGTTAACTTCAAAGGGAAAATTCCCGTTGAATTATAAATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCAATTGAGTATTGAGTATTAGCAAGACGTAGTAAGTATATGAGACAACTTCTACAATGGTTGAAGGAAGACGTTTTTGTAAGTAGCTATGCTGA  >  CP000730/263386‑263660
                                                                                                                                      |                                                                                                                                            
ggAAAACATTGGGCATTGCATATGAATTAGAAGATGTCGAAGCGCCCGATGATGAAGCTGTTAAAGCAGCCTACCGTTATGTTAACTTCAAAGGGAAAATTCCCGTTGAATTATAAATACAATTCAGACCGAAAAGAAAGc                                                                                                                                        >  2:123397/1‑141 (MQ=255)
  aaaaCATTGGGCATTGCATATGAATTAGAAGATGTCGAAGCGCCCGATGATGAAGCTGTTAAAGCAGCCTACCGTTATGTTAACTTCAAAGGGAAAATTCCCGTTGAATTATAAATACAATTCAGACCGAAAAGAAAGCat                                                                                                                                      >  1:253204/1‑141 (MQ=255)
     aCATTGGGCATTGCATATGAATTAGAAGATGTCGAAGCGCCCGATGATGAAGCTGTTAAAGCAGCCTACCGTTATGTTAACTTCAAAGGGAAAATTCCCGTTGAATTATAAATACAATTCAGACCGAAAAGAAAGCATATg                                                                                                                                   <  1:363403/141‑1 (MQ=255)
      cATTGGGCATTGCATATGAATTAGAAGATGTCGAAGCGCCCGATGATGAAGCTGTTAAAGCAGCCTACCGTTATGTTAACTTCAAAGGGAAAATTCCCGTTGAATTATAAATACAATTCAGACCGAAAAGAAGCATATGcc                                                                                                                                 <  1:323560/141‑2 (MQ=255)
       aTTGGGCATTGCATATGAATTAGAAGATGTCGAAGCGCCCGATGATGAAGCTGTTAAAGCAGCCTACCGTTATGTTAACTTCAAAGGGAAAATTCCCGTTGAATTATAAATACAATTCAGACCGAAAAGAAAGCATATGCa                                                                                                                                 <  2:126514/141‑1 (MQ=255)
        ttGGGCATTGCATATGAATTAGAAGATGTCGAAGCGCCCGATGATGAAGCTGTTAAAGCAGCCTACCGTTATGTTAACTTCAAAGGGAAAATTCCCGTTGAATTATAAATACAATTCAGACCGAAAAGAAAGCATATGCaa                                                                                                                                >  1:23238/1‑141 (MQ=255)
             cATTGCATATGAATTAGAAGATGTCGAAGCGCCCGATGATGAAGCTGTTAAAGCAGCCTACCGTTATGTTAACTTCAAAGGGAAAATTCCCGTTGAATTATAAATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCa                                                                                                                           >  1:171828/1‑141 (MQ=255)
               ttGCAGATGAATTAGAAGATGTCGAAGCGTCGGATGATGAAGCTGTTAAAGCAGTCTACCGTTATGTTAACTTCAAAGGGTAAATACCAGTTGAATTATAAATACATCTTATATCGAAAAGAAAGCTTATGCAAcataaaa                                                                                                                         >  2:179047/1‑135 (MQ=255)
                           tAGAAGATGTCGAAGCGCCCGATGATGAAGCTGTTAAAGCAGCCTACCGTTATGTTAACTTCAAAGGGAAAATTCCCGTTGAATTATAAATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCa                                                                                                             <  2:62564/141‑1 (MQ=255)
                             gaagaTGTCGAAGCGCCCGATGATGAAGCTGTTAAAGCAGCCTACCGTTATGTTAACTTCAAAGGGAAAATTCCCGTTGAATTATAAATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCata                                                                                                           >  1:151273/1‑141 (MQ=255)
                              aagaTGTCGAAGCGCCCGATGATGAAGCTGTTAAAGCAGCCTACCGTTATGTTAACTTCAAAGGGAAAATTCCCGTTGAATTATAAATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCatat                                                                                                          <  1:408750/141‑1 (MQ=255)
                              aagaTGTCGAAGCGCCCGATGATGAACGTGTTAATGCAGCCTACCGTTATGTTAACTTCAAAGGGAAAATTCCCGTTGAATTATAAATACAATTCGGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCatat                                                                                                          <  2:345888/141‑1 (MQ=255)
                               agaTGTCGAAGCGCCCGATGATGAAGCTGTTAAAGCAGCCTACCGTTATGTTAACTTCAAAGGGAAAATTCCCGTTGAATTATAAATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATg                                                                                                         <  1:178813/141‑1 (MQ=255)
                                  tGTCGAAGCGCCCGATGATTAATCTGTTAAAGCAGCCTACCGTTATGTTAACTTCAAAGGGAAAATTCCCGTTGAATTATAAATACAATTCAGACCGAAAATAAATCATATGCAACTTCAAGAGTGAAGGGGCATATGctt                                                                                                      <  2:106820/141‑1 (MQ=255)
                                   gTCGAAGCGCCCGATGATGAAGCTGTTAAAGCAGCCTACCGTTATGTTAACTTCAAAGGGAAAATTCCCGTTGAATTATAAATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGcttc                                                                                                     >  1:417097/1‑141 (MQ=255)
                                    tCGAAGCGCCCGATGATGAAGCTGTTAAAGCAGCCTACCGTTATGTTAACTTCAAAGGGAAAATTCCCGTTGAATTATAAATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGcttct                                                                                                    <  1:327611/141‑1 (MQ=255)
                                      gAAGCGCCCGATGATGAAGCTGTTAAAGCAGCCTACCGTTATGTTAACTTCAAAGGGAAAATTCCCGTTGAATTATAAATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCttt                                                                                                  <  1:277349/141‑1 (MQ=255)
                                      gAAGCGCCCGATGATGAAGCTGTTAAAGCAGCCTACCGTTATGTTAACTTCAAAGGGAAAATTCCCGTTGAATTATAAATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCttt                                                                                                  <  1:285391/141‑1 (MQ=255)
                                      gAAGCGCCCGATGATGAAGCTGTTAAAGCAGCCTACCGTTATGTTAACTTCAAAGGGAAAATTCCCGTTGAATTATAAATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCttt                                                                                                  >  2:264986/1‑141 (MQ=255)
                                       aaGCGCCCGATGATGAAGCTGTTAAAGCAGCCTACCGTTATGTTAACTTCAAAGGGAAAATTCCCGTTGAATTATAAATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCtttt                                                                                                 >  1:377272/1‑141 (MQ=255)
                                          cgcCCGATGATGAAGCTGTTAAAGCAGCCTACCGTTATGTTAACTTCAAAGGGAAAATTCCCGTTGAATTATAAATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCa                                                                                              >  1:121923/1‑141 (MQ=255)
                                           gcCCGATGATGAAGCTGTTAAAGCAGCCTACCGTTATGTTAACTTCAAAGGGAAAATTCCCGTTGAATTATAAATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCaa                                                                                             <  2:258701/141‑1 (MQ=255)
                                             ccGATGGTGAAGCTGTTAAAGCAGCCTACCGTTATGTTAACTTCAAAGGGAAAATTCCCGTTGAATTATAAATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGCGAAGGGGCATATGCTTCTTTTTCAatt                                                                                           >  2:399647/1‑141 (MQ=255)
                                              cGATGATGAAGCTGTTAAAGCAGCCTACCGTTATGTTAACTTCAAAGGGAAAATTCCCGTTGAATTATAAATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCAattg                                                                                          <  1:25404/141‑1 (MQ=255)
                                              cGATGATGAAGCTGTTAAAGCAGCCTACCGTTATGTTAACTTCAAAGGGAAAATTCCCGTTGAATTATAAATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCAattg                                                                                          <  1:377402/141‑1 (MQ=255)
                                                   atgaAGCTGTTAAAGCAGCCTACCGTTATGTTAACTTCAAAGGGAAAATTCCCGTTGAATTATAAATACAATTCAGACCGAGAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCAattgagtat                                                                                     <  1:254057/141‑1 (MQ=255)
                                                     gaAGCTGTTAAAGCAGCCTACCGTTATGTTAACTTCAAAGGGAAAATTCCCGTTGAATTATAAATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCAattgagtattg                                                                                   <  2:776/141‑1 (MQ=255)
                                                            ttACAGCAGCAAACCGTTATATTAACTAAAAAGGACAAATTCGCGGTGAATTATAAATACAATTCAGACCGAAAAGAAAGCCTTTGCAACTTCAAGATTTAAGAGACATATGCCTGTTCTTCAATGGAGTATTGAATAGTa                                                                            <  2:45175/141‑1 (MQ=255)
                                                             tAAAGCAGCCTACCGTTATGTTAACTTCAAAGGGAAAATTCCCGTTGAATTATAAATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCAATTGAGTATTGAGTATTAg                                                                           <  2:338937/141‑1 (MQ=255)
                                                               aaGCAGCCTACCGTTATGTTAACTTCAAAGGGAAAATTCCCGTTGAATTATAAATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCAATTGAGTATTGAGTATTAGCa                                                                         >  2:208481/1‑141 (MQ=255)
                                                                 gcagcCTACCGTTATGTTAACTTCAAAGGGAAAATTCCCGTTGAATTATAAATACAATTCAGACCGAAAAGAAAGCATAAGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCAATTGAGTATTGAGTATTAGCAAg                                                                       >  1:423783/1‑141 (MQ=255)
                                                                   agcCTACCGTTATGTTAACTTCAAAGGGAAAATTCCCGTTGAATTATAAATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCAATTGAGTATTGAGTATTAGCAAGAc                                                                     <  2:71081/141‑1 (MQ=255)
                                                                    gcCTACCGTTATGTTAACTTCAAAGGGAAAATTCCCGTTGAATTATAAATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCAATTGAGTATTGAGTATTAGCAAGACg                                                                    >  2:241429/1‑141 (MQ=255)
                                                                     ccTACCGTTATGTTAACTTCAAAGGGAAAATTCCCGTTGAATTATAAATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCAATTGAGTATTGAGTATTAGCAAGACgt                                                                   >  1:432278/1‑141 (MQ=255)
                                                                       tACCGTTATGTTAACTTCAAAGGGAAAATTCCCGTTGAATTATAAATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCAATTGAGTATTGAGTATTAGCAAGACgtag                                                                 <  1:339467/141‑1 (MQ=255)
                                                                         ccGTTATGTTAACTTCAAAGGGAAAATTCCCGTTGAATTATAAATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCAATTGAGTATTGAGTATTAGCAAGACgtagta                                                               <  1:338030/141‑1 (MQ=255)
                                                                         ccGTTATGTTAACTTCAAAGGGAAAATTCCCGTTGAATTATAAATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCAATTGAGTATTGAGTATTAGCAAGACgtagta                                                               >  1:292138/1‑141 (MQ=255)
                                                                          cGTTATGTTAACTTCAAAGGGAAAATTCCCGTTGAATTACAAATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTGCAATTGATTATTGAGTATTAGCAAGACGTagtaa                                                              >  2:345797/1‑141 (MQ=255)
                                                                           gTTATGTTAACTTCAAAGGGAAAATTCCCGTTGAATTATAAATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCAATTGAGTATTGAGTATTAGCAAGAAGTagtaag                                                             >  1:338419/1‑141 (MQ=255)
                                                                              aTGTTAACTTCAAAGGGAAAATTCCCGTTGAATTATAAATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCAATTGAGTATTGAGTATTAGCAAGACGTAGTAAGtat                                                          <  2:121923/141‑1 (MQ=255)
                                                                                  tAACTTCAAAGGGAAAATTCCCGTTGAATTATAAATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCaa                                                                                             <  1:235524/102‑1 (MQ=255)
                                                                                  tAACTTCAAAGGGAAAATTCCCGTTGAATTATAAATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCaa                                                                                             >  2:235524/1‑102 (MQ=255)
                                                                                           aGGGAAAATACCCGTTGAATTATACATACAACTCAGACTCAAAACAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTACTTTTTCAATTCAGTACTGAGTATTAGCAAGACGTACTACTCATCTGAGAAAACtcc                                             >  2:408515/1‑139 (MQ=255)
                                                                                            gggAAAATTCCCGTTGAATTATAAATACAATTCGGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCAATTGAGTATTGAGTATTAGCAAGACGTAGTAAGTATATGAGACAACTTCt                                            <  1:210347/141‑1 (MQ=255)
                                                                                                  aTTCCCGTTGAATTATAAATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCAATTGAGTATTGAGTATTAGCAAGACGTAGTAAGTATATGAGACAACTTCTACAATg                                      >  2:132648/1‑141 (MQ=255)
                                                                                                  aTTCCCGTTGAATTATAAATACAATTCAGACAGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCAATTGAGTATTGAGTATTAGCAAGACGTAGTAAGTATATGATACAACTTCTACAATg                                      >  2:342086/1‑141 (MQ=255)
                                                                                                      ccGTTGAATTATAAATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCAATTGAGTATTGAGTATTAGCAAGACGTAGTAAGTATATGAGACAACTTCTACAATGGTTg                                  <  2:253204/141‑1 (MQ=255)
                                                                                                      ccGTTGAATTATAAATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCAATTGAGTATTGAGTATTAGCAAGACGTAGTAAGTATATGAGACAACTTCTACAATGGTTg                                  >  2:115465/1‑141 (MQ=255)
                                                                                                            acttATAAATACAATTCAGACCGAAAAGAAAGCGTATGCAACTTCAAGAGTGAAGGGGCAAATGCTTCTTTTTCAATTGAGTATTGAGTATTAGCAAGACGTACTAAGTATATGAGACAACTTCTACAATGGTTgaaggaa                            >  2:371511/3‑141 (MQ=255)
                                                                                                            aaTTATAAATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCAATTGAGTATTGAGTATTAGCAAGACGTAGTAAGTATATGAGACAACTTCTACAATGGTTgaaggaa                            <  2:447088/141‑1 (MQ=255)
                                                                                                              ttATAAATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCAATTGAGTATTGAGTATTAGCAAGACGTAGTAAGTATATGAGACAACTTCTACAATGGTTGAAGGAAGa                          <  1:291580/141‑1 (MQ=255)
                                                                                                                ataAATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCAATTGAGTATTGAGTATTAGCAAGACGTAGTAAGTATATGAGACAACTTCTACAATGGTTGAAGGAAGACg                        <  2:435868/141‑1 (MQ=255)
                                                                                                                     tACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCAATTGAGTATTGAGTATTAGCAAGACGTAGTAAGTATATGAGACAACTTCTACAATGGTTGAAGGAAGACGttttt                   >  1:319979/1‑141 (MQ=255)
                                                                                                                       cAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCAATTGAGTATTGAGTATTAGCAAGACGTAGTAAGTATATGAGACAACTTCTACAATGGTTGAAGGAAGACGTTTTTGt                 <  1:356983/141‑1 (MQ=255)
                                                                                                                       cAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCAATTGAGTATTGAGTATTAGCAAGACCTAGTAAGTATATGAGACAACTTCTACAATGGTTGAAGGAAGACGTTTTTGt                 <  1:345797/141‑1 (MQ=255)
                                                                                                                             aGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCACTTGTGTATTGAGTATTAGCAAGACGTAGTAAGTATATGAGACAACTTCTACAATGGTTGAAGGAAGACGTTTTTGTAAGTAg           >  2:15541/1‑141 (MQ=255)
                                                                                                                                 cGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCAATTGAGTATTGAGTATTAGCAAGACGTAGTAAGTATATGAGACAACTTCTACAATGGTTGAAGGAAGACGTTTTTGTAAGTAGCTAt       >  2:150525/1‑141 (MQ=255)
                                                                                                                                      agaaagCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCAATTGAGTATTGAGTATTAGCAAGACGTAGTAAGTATATGAGACAACTTCTACAATGGTTGAAGGAAGACGTTTTTGTAAGTAGCTATGCTGa  >  1:270705/1‑141 (MQ=255)
                                                                                                                                      agaaagCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCAATTGAGTATTGAGTATTAGCAAGACGTAGTAAGTATATGAGACAACTTCTACAATGGTTGAAGGAAGACGTTTTTGTAAGTAGCTATGCTGa  >  1:355048/1‑141 (MQ=255)
                                                                                                                                      |                                                                                                                                            
GGAAAACATTGGGCATTGCATATGAATTAGAAGATGTCGAAGCGCCCGATGATGAAGCTGTTAAAGCAGCCTACCGTTATGTTAACTTCAAAGGGAAAATTCCCGTTGAATTATAAATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCAATTGAGTATTGAGTATTAGCAAGACGTAGTAAGTATATGAGACAACTTCTACAATGGTTGAAGGAAGACGTTTTTGTAAGTAGCTATGCTGA  >  CP000730/263386‑263660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.