New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	CP000730	= 263520	58 (1.490)	4 (0.110)	4/268	5.4	7.1%	intergenic (+19/‑85)	pflA/USA300HOU_0235	[formate‑C‑acetyltransferase]‑activating enzyme/hypothetical protein
	?	CP000730	= 263562	49 (1.310)					intergenic (+61/‑43)	pflA/USA300HOU_0235	[formate‑C‑acetyltransferase]‑activating enzyme/hypothetical protein
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TCGAAGCGCCCGATGATGAAGCTGTTAAAGCAGCCTACCGTTATGTTAACTTCAAAGGGAAAATTCCCGTTGAATTATAAATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCAATTGAGTATTGAGTATTAGCAAGACGTAGTAAGTATATGAGACAACTTCTACAATGGTTGAAGGAAGACGTTTTTGTAAGTAGCTATGCTGATAAAGAATGTGATGTCTTGTTAAAGGTG  >  CP000730/263422‑263688                                                                                                                                             |                                                                                                                               tCGAAGCGCCCGATGATGAAGCTGTTAAAGCAGCCTACCGTTATGTTAACTTCAAAGGGAAAATTCCCGTTGAATTATAAATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGcttct                                                                                                                                <  1:327611/141‑1 (MQ=255)   gAAGCGCCCGATGATGAAGCTGTTAAAGCAGCCTACCGTTATGTTAACTTCAAAGGGAAAATTCCCGTTGAATTATAAATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCttt                                                                                                                              <  1:285391/141‑1 (MQ=255)   gAAGCGCCCGATGATGAAGCTGTTAAAGCAGCCTACCGTTATGTTAACTTCAAAGGGAAAATTCCCGTTGAATTATAAATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCttt                                                                                                                              <  1:277349/141‑1 (MQ=255)   gAAGCGCCCGATGATGAAGCTGTTAAAGCAGCCTACCGTTATGTTAACTTCAAAGGGAAAATTCCCGTTGAATTATAAATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCttt                                                                                                                              >  2:264986/1‑141 (MQ=255)    aaGCGCCCGATGATGAAGCTGTTAAAGCAGCCTACCGTTATGTTAACTTCAAAGGGAAAATTCCCGTTGAATTATAAATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCtttt                                                                                                                             >  1:377272/1‑141 (MQ=255)       cgcCCGATGATGAAGCTGTTAAAGCAGCCTACCGTTATGTTAACTTCAAAGGGAAAATTCCCGTTGAATTATAAATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCa                                                                                                                          >  1:121923/1‑141 (MQ=255)        gcCCGATGATGAAGCTGTTAAAGCAGCCTACCGTTATGTTAACTTCAAAGGGAAAATTCCCGTTGAATTATAAATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCaa                                                                                                                         <  2:258701/141‑1 (MQ=255)          ccGATGGTGAAGCTGTTAAAGCAGCCTACCGTTATGTTAACTTCAAAGGGAAAATTCCCGTTGAATTATAAATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGCGAAGGGGCATATGCTTCTTTTTCAatt                                                                                                                       >  2:399647/1‑141 (MQ=255)           cGATGATGAAGCTGTTAAAGCAGCCTACCGTTATGTTAACTTCAAAGGGAAAATTCCCGTTGAATTATAAATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCAattg                                                                                                                      <  1:377402/141‑1 (MQ=255)           cGATGATGAAGCTGTTAAAGCAGCCTACCGTTATGTTAACTTCAAAGGGAAAATTCCCGTTGAATTATAAATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCAattg                                                                                                                      <  1:25404/141‑1 (MQ=255)                atgaAGCTGTTAAAGCAGCCTACCGTTATGTTAACTTCAAAGGGAAAATTCCCGTTGAATTATAAATACAATTCAGACCGAGAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCAattgagtat                                                                                                                 <  1:254057/141‑1 (MQ=255)                  gaAGCTGTTAAAGCAGCCTACCGTTATGTTAACTTCAAAGGGAAAATTCCCGTTGAATTATAAATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCAattgagtattg                                                                                                               <  2:776/141‑1 (MQ=255)                         ttACAGCAGCAAACCGTTATATTAACTAAAAAGGACAAATTCGCGGTGAATTATAAATACAATTCAGACCGAAAAGAAAGCCTTTGCAACTTCAAGATTTAAGAGACATATGCCTGTTCTTCAATGGAGTATTGAATAGTa                                                                                                        <  2:45175/141‑1 (MQ=255)                          tAAAGCAGCCTACCGTTATGTTAACTTCAAAGGGAAAATTCCCGTTGAATTATAAATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCAATTGAGTATTGAGTATTAg                                                                                                       <  2:338937/141‑1 (MQ=255)                            aaGCAGCCTACCGTTATGTTAACTTCAAAGGGAAAATTCCCGTTGAATTATAAATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCAATTGAGTATTGAGTATTAGCa                                                                                                     >  2:208481/1‑141 (MQ=255)                              gcagcCTACCGTTATGTTAACTTCAAAGGGAAAATTCCCGTTGAATTATAAATACAATTCAGACCGAAAAGAAAGCATAAGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCAATTGAGTATTGAGTATTAGCAAg                                                                                                   >  1:423783/1‑141 (MQ=255)                                agcCTACCGTTATGTTAACTTCAAAGGGAAAATTCCCGTTGAATTATAAATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCAATTGAGTATTGAGTATTAGCAAGAc                                                                                                 <  2:71081/141‑1 (MQ=255)                                 gcCTACCGTTATGTTAACTTCAAAGGGAAAATTCCCGTTGAATTATAAATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCAATTGAGTATTGAGTATTAGCAAGACg                                                                                                >  2:241429/1‑141 (MQ=255)                                  ccTACCGTTATGTTAACTTCAAAGGGAAAATTCCCGTTGAATTATAAATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCAATTGAGTATTGAGTATTAGCAAGACgt                                                                                               >  1:432278/1‑141 (MQ=255)                                    tACCGTTATGTTAACTTCAAAGGGAAAATTCCCGTTGAATTATAAATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCAATTGAGTATTGAGTATTAGCAAGACgtag                                                                                             <  1:339467/141‑1 (MQ=255)                                      ccGTTATGTTAACTTCAAAGGGAAAATTCCCGTTGAATTATAAATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCAATTGAGTATTGAGTATTAGCAAGACgtagta                                                                                           >  1:292138/1‑141 (MQ=255)                                      ccGTTATGTTAACTTCAAAGGGAAAATTCCCGTTGAATTATAAATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCAATTGAGTATTGAGTATTAGCAAGACgtagta                                                                                           <  1:338030/141‑1 (MQ=255)                                       cGTTATGTTAACTTCAAAGGGAAAATTCCCGTTGAATTACAAATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTGCAATTGATTATTGAGTATTAGCAAGACGTagtaa                                                                                          >  2:345797/1‑141 (MQ=255)                                        gTTATGTTAACTTCAAAGGGAAAATTCCCGTTGAATTATAAATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCAATTGAGTATTGAGTATTAGCAAGAAGTagtaag                                                                                         >  1:338419/1‑141 (MQ=255)                                           aTGTTAACTTCAAAGGGAAAATTCCCGTTGAATTATAAATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCAATTGAGTATTGAGTATTAGCAAGACGTAGTAAGtat                                                                                      <  2:121923/141‑1 (MQ=255)                                               tAACTTCAAAGGGAAAATTCCCGTTGAATTATAAATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCaa                                                                                                                         <  1:235524/102‑1 (MQ=255)                                               tAACTTCAAAGGGAAAATTCCCGTTGAATTATAAATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCaa                                                                                                                         >  2:235524/1‑102 (MQ=255)                                                        aGGGAAAATACCCGTTGAATTATACATACAACTCAGACTCAAAACAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTACTTTTTCAATTCAGTACTGAGTATTAGCAAGACGTACTACTCATCTGAGAAAACtcc                                                                         >  2:408515/1‑139 (MQ=255)                                                         gggAAAATTCCCGTTGAATTATAAATACAATTCGGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCAATTGAGTATTGAGTATTAGCAAGACGTAGTAAGTATATGAGACAACTTCt                                                                        <  1:210347/141‑1 (MQ=255)                                                               aTTCCCGTTGAATTATAAATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCAATTGAGTATTGAGTATTAGCAAGACGTAGTAAGTATATGAGACAACTTCTACAATg                                                                  >  2:132648/1‑141 (MQ=255)                                                               aTTCCCGTTGAATTATAAATACAATTCAGACAGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCAATTGAGTATTGAGTATTAGCAAGACGTAGTAAGTATATGATACAACTTCTACAATg                                                                  >  2:342086/1‑141 (MQ=255)                                                                   ccGTTGAATTATAAATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCAATTGAGTATTGAGTATTAGCAAGACGTAGTAAGTATATGAGACAACTTCTACAATGGTTg                                                              >  2:115465/1‑141 (MQ=255)                                                                   ccGTTGAATTATAAATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCAATTGAGTATTGAGTATTAGCAAGACGTAGTAAGTATATGAGACAACTTCTACAATGGTTg                                                              <  2:253204/141‑1 (MQ=255)                                                                         acttATAAATACAATTCAGACCGAAAAGAAAGCGTATGCAACTTCAAGAGTGAAGGGGCAAATGCTTCTTTTTCAATTGAGTATTGAGTATTAGCAAGACGTACTAAGTATATGAGACAACTTCTACAATGGTTgaaggaa                                                        >  2:371511/3‑141 (MQ=255)                                                                         aaTTATAAATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCAATTGAGTATTGAGTATTAGCAAGACGTAGTAAGTATATGAGACAACTTCTACAATGGTTgaaggaa                                                        <  2:447088/141‑1 (MQ=255)                                                                           ttATAAATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCAATTGAGTATTGAGTATTAGCAAGACGTAGTAAGTATATGAGACAACTTCTACAATGGTTGAAGGAAGa                                                      <  1:291580/141‑1 (MQ=255)                                                                             ataAATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCAATTGAGTATTGAGTATTAGCAAGACGTAGTAAGTATATGAGACAACTTCTACAATGGTTGAAGGAAGACg                                                    <  2:435868/141‑1 (MQ=255)                                                                                  tACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCAATTGAGTATTGAGTATTAGCAAGACGTAGTAAGTATATGAGACAACTTCTACAATGGTTGAAGGAAGACGttttt                                               >  1:319979/1‑141 (MQ=255)                                                                                    cAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCAATTGAGTATTGAGTATTAGCAAGACGTAGTAAGTATATGAGACAACTTCTACAATGGTTGAAGGAAGACGTTTTTGt                                             <  1:356983/141‑1 (MQ=255)                                                                                    cAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCAATTGAGTATTGAGTATTAGCAAGACCTAGTAAGTATATGAGACAACTTCTACAATGGTTGAAGGAAGACGTTTTTGt                                             <  1:345797/141‑1 (MQ=255)                                                                                          aGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCACTTGTGTATTGAGTATTAGCAAGACGTAGTAAGTATATGAGACAACTTCTACAATGGTTGAAGGAAGACGTTTTTGTAAGTAg                                       >  2:15541/1‑141 (MQ=255)                                                                                              cGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCAATTGAGTATTGAGTATTAGCAAGACGTAGTAAGTATATGAGACAACTTCTACAATGGTTGAAGGAAGACGTTTTTGTAAGTAGCTAt                                   >  2:150525/1‑141 (MQ=255)                                                                                                   agaaagCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCAATTGAGTATTGAGTATTAGCAAGACGTAGTAAGTATATGAGACAACTTCTACAATGGTTGAAGGAAGACGTTTTTGTAAGTAGCTATGCTGa                              >  1:270705/1‑141 (MQ=255)                                                                                                   agaaagCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCAATTGAGTATTGAGTATTAGCAAGACGTAGTAAGTATATGAGACAACTTCTACAATGGTTGAAGGAAGACGTTTTTGTAAGTAGCTATGCTGa                              >  1:355048/1‑141 (MQ=255)                                                                                                       agCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCAATTGAGTATTGAGTATTAGCAAGACGTAGTAAGTATATGAGACAACTTCTACTATGGTTGAAGGAAGACGTTTTTGTAAGTAGCTATGCTGATaaa                          <  2:153920/141‑1 (MQ=255)                                                                                                               cAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCAATTGAGTATTGAGTATTAGCAAGACGTAGTAAGTATATGAGACAACTTCTACAATGGTTGAAGGAAGACGTTTTTGTAAGTAGCTATGCTGATAAAGAATGTGa                  <  2:377272/141‑1 (MQ=255)                                                                                                                  cTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCAATTGAGTATTGAGTATTAGCAAGACGTAGTAAGTATATGAGACAACTGCTACAATGGTTGAAGGAAGACGTTTTTGTAAGTAGCTATGCTGATAAAGAATGTGATgg               <  1:116254/141‑2 (MQ=255)                                                                                                                     cAAGAGTGAAGGGGCATATGCTTCTTTTTCAATTGAGTATTGAGTATTAGCAAGACGTAGTAAGTATATGAGACAACTTCTACAATTGTTGAAGGAAGACGTTTTTGTAAGTAGCTATGCTGATAAAGAATGTGATGTCtt            >  1:448430/1‑141 (MQ=255)                                                                                                                     cAAGAGTGAAGGGGCATATGCTTCTTTTTCAATTGAGTATTGAGTATTAGCAAGACGTAGTAAGTATATGAGACAACTTCTACAATGGTTGAAGGAAGACGTTTTTGTAAGTAGCTATGCTGATAAAGAATGTGATGTCtt            >  1:448111/1‑141 (MQ=255)                                                                                                                      actcttGAAGTGGCATATGCTTCTTTTTCAATTGAGTATTGAGTATTAGCAAGACGTAGTAAGTATATGAGACAACTTCTACAATGGTTGAAGGAAGACGCTTTTGTAAGTAGCTATGCTGATAAAGAATGTGATGTCTTg           >  1:74758/6‑141 (MQ=255)                                                                                                                      aaGAGTGAAGGGGCATATGCTTCTTTTTCAATTGAGTATTGAGTATTAGCAAGACGTAGTAAGTATATGAGACAACTTCTACAATGGTTGAAGGAAGACGTGTTTGTAAGTAGCTATGCTGATAAAGAATGTGATGTCTTg           >  2:60279/1‑141 (MQ=255)                                                                                                                           tGAAGGGGCATATGCTTCTTTTTCAATTGAGTATTGAGTATTAGCAAGACGTAGTAAGTATATGAGACAACTTCTACAATGGTTGAAGGAAGACGTTTTTGTAAGTAGCTATGCTGATAAAGAATGTGATGTCTTGTTaaa      >  1:211419/1‑141 (MQ=255)                                                                                                                               ttttgatttttatcttTTTCAATTGAGTATTGAGTATTAGCAAGACGTAGTAAGTATATGAGACAACTTCTACAATGGTTGAAGGAAGACGTTTTTGTAAGTAGCTATGCTGATAAAGAATGTGATGTCTTGTTAAAGGTg  <  1:189891/129‑1 (MQ=255)                                                                                                                               ggggCATATGCTTCTTTTTCAGTTTAGGATTGAGTATTAGCAAGACGTCGTAAGTATATGAGACAACTTCTCCAAGGGTAGAAGGAAGGCGTTTTTGTAAGTAGCTATGCTGATAAAGACTGTGATGTCTGGTTAAAGGTg  >  2:394278/1‑141 (MQ=255)                                                                                                                                             |                                                                                                                               TCGAAGCGCCCGATGATGAAGCTGTTAAAGCAGCCTACCGTTATGTTAACTTCAAAGGGAAAATTCCCGTTGAATTATAAATACAATTCAGACCGAAAAGAAAGCATATGCAACTTCAAGAGTGAAGGGGCATATGCTTCTTTTTCAATTGAGTATTGAGTATTAGCAAGACGTAGTAAGTATATGAGACAACTTCTACAATGGTTGAAGGAAGACGTTTTTGTAAGTAGCTATGCTGATAAAGAATGTGATGTCTTGTTAAAGGTG  >  CP000730/263422‑263688

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.