New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP000730 82395 =137 (1.980)8 (0.120) 4/292 2.1 5.7% coding (500/957 nt) nikA nickel (Ni2+)/peptide ABC superfamily ATP binding cassette transporter, membrane protein
?CP000730 82432 = 132 (1.960)coding (537/957 nt) nikA nickel (Ni2+)/peptide ABC superfamily ATP binding cassette transporter, membrane protein
Rejected: Frequency below/above cutoff threshold.

CGCGCTTTTAACGAAAAATGTCTTAACTGCGCCAGTTGATACGCATCTTGATCTAACGTTTGTGTAATCAGATGTGCTGTCATCAATAAGATATGACACCCTTCTATAACAACAATTGTGAAGATTGGCAGCACAAAGTGTTGCCATGAATCAC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  CP000730/82548‑82395
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tcacAATTGTTGTTATAGAAGGGTGTCATATCTTATTGATGACAGCACATCTGATTACACAAACGTTAGATCAAGATGCGTATCAACTGGCGCAGTTAAGACATTTTTCGTTAAAAGCGCGTATCATCGTACAAATTAAAGAGATATTTGCACC  >  CP000730/82432‑82581
                                                                                                                                                                                                                                                                                                                
CGCGCTTTTAACGAAAAATGTCTTAACTGCGCCAGTTGATACGCATCTTGATCTAACGTTTGTGTAATCAGATGTGCTGTCATCAATAAGATATGACACCCTTCTATAACAACAATTGTGAAGATTGGCAGCACAAAGTGTTGCCATGAAT                                                                                                                                                           >  2:920000/1‑151
CGCGCTTTTAACGAAAAATGTCTTAACTGCGCCAGTTGATACGCATCTTGATCTAACGTTTGTGTAATCAGATGTGCTGTCATCAATAAGATATGACACCCTTCTATAACAACAATTGTGAAGATTGGCAGCACAAAGTGTTGCCATGAAT                                                                                                                                                           >  2:23761/1‑151
CGCGCTTTTAACGAAAAATGTCTTAACTGCGCCAGTTGATACGCATCTTGATCTAACGTTTGTGTAATCAGATGTGCTGTCATCAATAAGATATGACACCCTTCTATAACAACAATTGTGAAGATTGGCAGCACAAAGTGTTGCCATGAAT                                                                                                                                                           <  1:275800/151‑1
  CGCTTTTAACGAAAAATGTCTTAACTGCGCCAGTTGATACGCATCTTGATCTAACGTTTGTGTAATCAGATGTGCTGTCATCAATAAGATATGACACCCTTCTATAACAACAATTGTGAAGATTGGCAGCACAAAGTGTTGCCATGAATCA                                                                                                                                                         >  2:16970/1‑151
   GCTTTTAACGAAAAATGTCTTAACTGCGCCAGTTGATACGCATCTTGATCTAACGTTTGTGTAATCAGATGTGCTGTCATCAATAAGATATGACACCCTTCTATAACAACAATTGTGAAGATTGGCAGCACAAAGTGTTGCCATGAATCAC                                                                                                                                                        <  1:35037/151‑1
   GCTTTTAACGAAAAATGTCTTAACTGCGCCAGTTGATACGCATCTTGATCTAACGTTTGTGTAATCAGATGTGCTGTCATCAATAAGATATGACACCCTTCTATAACAACAATTGTGAAGATTGGCAGCACAAAGTGTTGCCATGAATCAC                                                                                                                                                        <  1:35224/151‑1
   GCTTTTAACGAAAAATGTCTTAACTGCGCCAGTTGATACGCATCTTGATCTAACGTTTGTGTAATCAGATGTGCTGTCATCAATAAGATATGACACCCTTCTATAACAACAATTGTGAAGATTGGCAGCACAAAGTGTTGCCATGAATCAC                                                                                                                                                        <  2:465417/151‑1
                                                                                                        TATAACAACAATTGTGAAGATTGGCAGCACAAAGTGTTGCCATGAATCACAATTGTTGTTATAGAAGGGTGTCATATCTTATTGATGACAGCACATCTGATTACACAAACGTTAGATCAAGATGCGTATCAACTGGCGCAGTTAAGACATT                                                   >  2:896068/1‑151
                                                                                                        TATAACAACAATTGTGAAGATTGGCAGCACAAAGTGTTGCCATGAATCACAATTGTTGTTATAGAAGGGTGTCATATCTTATTGATGACAGCACATCTGATTACACAAACGTTAGATCAAGATGCGTATCAACTGGCGCAGTTAAGACATT                                                   >  1:892126/1‑151
                                                                                                            ACAACAATTGTGAAGATTGGCAGCACAAAGTGTTGCCATGAATCACAATTGTTGTTATAGAAGGGTGTCATATCTTATTGATGACAGCACATCTGATTACACAAACGTTAGATCAAGATGCGTATCAACTGGCGCAGTTAAGACATTTTTC                                               >  2:984756/1‑151
                                                                                                            ACAACAATTGTGAAGATTGGCAGCACAAAGTGTTGCCATGAATCACAATTGTTGTTATAGAAGGGTGTCATATCTTATTGATGACAGCACATCTGATTACACAAACGTTAGATCAAGATGCGTATCAACTGGCGCAGTTAAGACATTTTTC                                               >  2:359881/1‑151
                                                                                                            ACAACAATTGTGAAGATTGGCAGCACAAAGTGTTGCCATGAATCACAATTGTTGTTATAGAAGGGTGTCATATCTTATTGATGACAGCACATCTGATTACACAAACGTTAGATCAAGATGCGTATCAACTGGCGCAGTTAAGACATTTTTC                                               >  2:868084/1‑151
                                                                                                                           ATTGGCAGCACAAAGTGTTGCCAATCTTCACAATTGTTGTTATAGAAGGGTGTCATATCTTATTGATGACAGCACATCTGATTACACAAACGTTAGATCAAGATGCGTATCAACTGGCGCAGTTAAGACATTTTTCTTTtttttcgcgtat                                >  2:356657/1‑139
                                                                                                                           ATTGGCAGCACAAAGTGTTGCCAATATTCACAATTGTTGTTATAGAAGGGTGTCATATCTTATTGATGACAGCACATCTGATTACACAAACGTTAGATCAAGATGCGTATCAACTGGCGCAGTTAAGACATTTTTCGTTAAAAGCGCGTAT                                >  1:269076/1‑151
                                                                                                                            TTGGCAGCACAAAGTGTTGCCAATCTTCACAATTGTTGTTATAGAAGGGTGTCATATCTTATTGATGACAGCACATCTGATTACACAAACGTTAGATCAAGATGCGTATCAACTGGCGCAGTTAAGACATTTTTCGTTAAAAGCGCGTATC                               >  2:892194/1‑151
                                                                                                                                                      TCACAATTGTTGTTATAGAAGGGTGTCATATCTTATTGATGACAGCACATCTGATTACACAAACGTTAGATCAAGATGCGTATCAACTGGCGCAGTTAAGACATTTTTCGTTAAAAGCGCGTATCATCGTACAAATTAAAGAGATATTTGC     <  2:401695/151‑1
                                                                                                                                                       CACAATTGTTGTTATAGAAGGGTGTCATATCTTATTGATGACAGCACATCTGATTACACAAACGTTAGATCAAGATGCGTATCAACTGGCGCAGTTAAGACATTTTTCGTTAAAAGCGCGTATCATCGTACAAATTAAAGAGATATTTGCA    >  2:94851/1‑151
                                                                                                                                                       CACAATTGTTGTTATAGAAGGGTGTCATATCTTATTGATGACAGCACATCTGATTACACAAACGTTAGATCAAGATGCGTATCAACTGGCGCAGTTAAGACATTTTTCGTTAAAAGCGCGTATCATCGTACAAATTAAAGAGATATTTGCA    >  2:606078/1‑151
                                                                                                                                                       CACAATTGTTGTTATAGAAGGGTGTCATATCTTATTGATGACAGCACATCTGATTACACAAACGTTAGATCAAGATGCGTATCAACTGGCGCAGTTAAGACATTTTTCGTTAAAAGCGCGTATCATCGTACAAATTAAAGAGATATTTGCA    >  2:94845/1‑151
                                                                                                                                                         CAATTGTTGTTATAGAAGGGTGTCATATCTTATTGATGACAGCACATCTGATTACACAAACGTTAGATCAAGATGCGTATCAACTGGCGCAGTTAAGACATTTTTCGTTAAAAGCGCGTATCATCGTACAAATTAAAGAGATATTTGCACC  <  1:494648/151‑1
                                                                                                                                                         CAATTGTTGTTATAGAAGGGTGTCATATCTTATTGATGACAGCACATCTGATTACACAAACGTTAGATCAAGATGCGTATCAACTGGCGCAGTTAAGACATTTTTCGTTAAAAGCGCGTATCATCGTACAAATTAAAGAGATATTTGCACC  <  1:243584/151‑1
                                                                                                                                                         CAATTGTTGTTATAGAAGGGTGTCATATCTTATTGATGACAGCACATCTGATTACACAAACGTTAGATCAAGATGCGTATCAACTGGCGCAGTTAAGACATTTTTCGTTAAAAGCGCGTATCATCGTACAAATTAAAGAGATATTTGCACC  >  2:746991/1‑151
                                                                                                                                                         CAATTGTTGTTATAGAAGGGTGTCATATCTTATTGATGACAGCACATCTGATTACACAAACGTTAGATCAAGATGCGTATCAACTGGCGCAGTTAAGACATTTTTCGTTAAAAGCGCGTATCATCGTACAAATTAAAGAGATATTTGCACC  <  2:831895/151‑1
                                                                                                                                                         CAATTGTTGTTATAGAAGGGTGTCATATCTTATTGATGACAGCACATCTGATTACACAAACGTTAGATCAAGATGCGTATCAACTGGCGCAGTTAAGACATTTTTCGTTAAAAGCGCGTATCATCGTACAAATTAAAGAGATATTTGCACC  <  1:108483/151‑1
                                                                                                                                                                                                                                                                                                                
CGCGCTTTTAACGAAAAATGTCTTAACTGCGCCAGTTGATACGCATCTTGATCTAACGTTTGTGTAATCAGATGTGCTGTCATCAATAAGATATGACACCCTTCTATAACAACAATTGTGAAGATTGGCAGCACAAAGTGTTGCCATGAATCAC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  CP000730/82548‑82395
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tcacAATTGTTGTTATAGAAGGGTGTCATATCTTATTGATGACAGCACATCTGATTACACAAACGTTAGATCAAGATGCGTATCAACTGGCGCAGTTAAGACATTTTTCGTTAAAAGCGCGTATCATCGTACAAATTAAAGAGATATTTGCACC  >  CP000730/82432‑82581

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.