New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP000730 1409145 =109 (1.580)3 (0.050) 3/278 2.2 2.9% coding (410/1215 nt) trpB tryptophan synthase beta subunit
?CP000730 1409186 = 103 (1.610)coding (451/1215 nt) trpB tryptophan synthase beta subunit
Rejected: Frequency below/above cutoff threshold.

ATCATCTACATGACTTACCCAATATTGCAATGCTTTATTAACTGCATCCGATAAAGTCCCTTGACCATCTTCAACTGCCACAACCTTTGCACCAAGTAATTCCATTCTAAATACATTAAGTTGTTGTCTTTTAATATCTTCACTTCCCATAAAGACAACAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  CP000730/1409305‑1409145
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑aaagacaacaaCTTAATGTATTTAGAATGGAATTACTTGGTGCAAAGGTTGTGGCAGTTGAAGATGGTCAAGGGACTTTATCGGATGCAGTTAATAAAGCATTGCAATATTGGGTAAGTCATGTAGATGATACACATTATTTATTAGGTTCTGCATTAGGT  >  CP000730/1409186‑1409335
                                                                                                                                                                                                                                                                                                                       
ATCATCTACATGACTTACCCAATATTGCAATGCTTTATTAACTGCATCCGATAAAGTCCCTTGACCATCTTCAACTGCCACAACCTTTGCACCAAGTAATTCCATTCTAAATACATTAAGTTGTTGTCTTTTAATATCTTCACTTCCCATA                                                                                                                                                                  <  1:166704/151‑1
ATCATCTACATGACTTACCCAATATTGCAATGCTTTATTAACTGCATCCGATAAAGTCCCTTGACCATCTTCAACTGCCACAACCTTTGCACCAAGTAATTCCATTCTAAATACATTAAGTTGTTGTCTTTGAATATCTTCACTTCCCATA                                                                                                                                                                  <  1:933627/151‑1
   ATCTACATGACTTACCCAATATTGCAATGCTTTATTAACTGCATCCGATAAAGTCCCTTGACCATCTTCAACTGCCACAACCTTTGCACCAAGTAATTCCATTCTAAATACATTAAGTTGTTGTCTTTTAATATCTTCACTTCCCATAAAG                                                                                                                                                               <  1:698051/151‑1
   ATCTACATGACTTACCCAATATTGCAATGCTTTATTAACTGCATCCGATAAAGTCCCTTGACCATCTTCAACTGCCACAACCTTTGCACCAAGTAAATCCATTCTAAATACATTAAGTTGTTGTCTTTTAATATCTTCACTTCCCATAAAG                                                                                                                                                               >  2:592566/1‑151
    TCTACATGACTTACCCAATATTGCAATGCTTTATTAACTGCATCCGATAAAGTCCCTTGACCATCTTCAACTGCCACAACCTTTGCACAGAGTAATTCCATTCTAAATACATTAAGTTGTTGTCTTTTAATATCTTCACTTCCCATAAAGA                                                                                                                                                              >  1:818945/1‑151
     CTACATGACTTACCCAATATTGCAATGCTTTATTAACTGCATCCGATAAAGTCCCTTGACCATCTTCAACTGCCACAACCTTTGCACCAAGTAATTCCATTCTAAATACATTAAGTTGTTGTCTTTTAATATCTTCACTTCCCATAAAGAC                                                                                                                                                             <  1:780672/151‑1
       ACATGACTTACCCAATATTGCAATGCTTTATTAACTGCATCCGATAAAGTCCCTTGACCATCTTCAACTGCCACAACCTTTGCACCAAGTAATTCCATTCTAAATACATTAAGTTGTTGTCTTTTAATATCTTCACTTCCCATAAAGACAA                                                                                                                                                           >  2:85419/1‑151
       ACATGACTTACCCAATATTGCAATGCTTTATTAACTGCATCCGATAAAGTCCCTTGACCATCTTCAACTGCCACAACCTTTGCACCAAGTAATTCCATTCTAAATACATTAAGTTGTTGTCTTTTAATATCTTCACTTCCCATAAAGACAA                                                                                                                                                           <  1:207512/151‑1
         ATGACTTACCCAATATTGCAATGCTTTATTAACTGCATCCGATAAAGTCCCTTGACCATCTTCAACTGCCACAGCCTTTGCACCAAGTAATTCCATTCTAAATACATTAAGTTGTTGTCTTTTAATATCTTCACTTCCCATAAAGACAACA                                                                                                                                                         <  1:86175/151‑1
         ATGACTTACCCAATATTGCAATGCTTTATTAACTGCATCCGATAAAGTCCCTTGACCATCTTCAACTGCCACAACCTTTGCACCAAGTAATTCCATTCTAAATACATTAAGTTGTTGTCTTTTAATATCTTCACTTCCCATAAAGACAACA                                                                                                                                                         <  2:261458/151‑1
          TGACTTACCCAATATTGCAATGCTTTATTAACTGCATCCGATAAAGTCCCTTGACCATCTTCAACTGCCACAACCTTTGCACCAAGTAATTCCATTCTAAATACATTAAGTTGTTGTCTTTTAATACCTTCACTTCCCATAAAGACAACAA                                                                                                                                                        >  2:1042003/1‑151
                                                                                                                 CATTAAGTTGTTGTCTTTTAATATCTTCACTTCCCATAAAGACAACAACTTAATGTATTTAGAATGGAATTACTTGGTGCAAAGGTTGTGGCAGTTGAAGATGGTCAAGGGACTTTATCGGATGCAGTTAATAAAGCATTGCAATATTGGG                                                 >  1:481125/1‑151
                                                                                                                   TTAAGTTGTTGTCTTTTAATATCTTCACTTCCCATAAAGACAACAACTTAATGTATTTAGAATGGAATTACTTGGTGCAAAGGTTGTGGCAGTTGAAGATGGTCAAGGGACTTTATCGGATGCAGTTAATAAAGCATTGCAATATTGGGTA                                               >  1:707434/1‑151
                                                                                                                             GTCTTTTAATATCTTCACTTCCCATAAAGACAACAACTTAATGTATTTAGAATGGAATTACTTGGTGCAAAGGTTGTGGCAGTTGAAGATGGTCAAGGGACTTTATCGGATGCAGTTAATAAAGCATTGCAAAATTGGGTAAGTCATGTAG                                     >  1:286527/1‑151
                                                                                                                                                      AAAGACAACAACTTAATGTATTTAGAATGGAATTACTTGGTGCAAAGGTTGTGGCAGTTGAAGATGGTCAAGGGACTTTATCGGATGCAGTTAATAAAGCATTGCAATATTGGGTAAGTCATGTAGATGATACACATTATTTATTAGGTTC            <  2:979869/151‑1
                                                                                                                                                       AAGACAACAACTTAATGTATTTAGAATGGAATTACTTGGTGCAAAGGTTGTGGCAGTTGAAGATGGTCAAGGGACTTTATCGGATGCAGTTAATAAAGCATTGCAATATTGGGTAAGTCATGTAGATGATACACATTATTTATTAGGTTCT           >  2:491900/1‑151
                                                                                                                                                         GACAACAACTTAATGTATTTAGAATGGAATTACTTGGTGCAAAGGTTGTGGCAGTTGAAGATGGTCAAGGGACTTTATCGGATGCAGTTAATAAAGCATTGCAATATTGGGTAAGTCATGTAGATGATACACATTATTTATTAGGTTATGC         >  2:1045521/1‑151
                                                                                                                                                           CAACAACTTAATGTATTTATAATGGAATTACTTGGTGCAAAGGTTGTGGCAGTTGAAGATGGTCAAGGGACTTTATCGGATGCAGTTAATAAAGCATTGCAATATTGGGTAAGTCATGTAGATGATACACATTATTTATTAGGTTCTGCAT       >  1:932224/1‑151
                                                                                                                                                           CAACAACTTAATGTATTTAGAATGGAATTACTTGGTGCAAAGGTTGTGGCAGTTGAAGATGGTCAAGGGACTTTATCGGATGCAGTTAATAAAGCATTGCAATATTGGGTAAGTCATGTAGATGATACACATTATTTATTAGGTTCTGCAT       >  2:286590/1‑151
                                                                                                                                                           CAACAACTTAATGTATTTAGAATGGAATTACTTGGTGCAAAGGTTGTGGCAGTTGAAGATGGTCAAGGGACTTTATCGGATGCAGTTAATAAAGCATTGCAATATTGGGTAAGTCATGTAGATGATACACATTATTTATTAGGTTCTGCAT       >  1:396621/1‑151
                                                                                                                                                           CAACAACTTAATGTATTTAGAATGGAATTACTTGGTGCAAAGGTTGTGGCAGTTGAAGATGGTCAAGGGACTTTATCGGATGCAGTTAATAAAGCATTGCAATATTGGGTAAGTCATGTAGATGATACACATTATTTATTAGGTTCTGCAT       >  1:633784/1‑151
                                                                                                                                                             ACAACTTAATGTATTTAGAATGGAATTACTTGGTGCAAAGGTTGTGGCAGTTGAAGATGGTCAAGGGACTTTATCGGATGCAGTTAATAAAGCATTGCAATATTGGGTAAGTCATGTAGATGATACACATTATTTATTAGGTTCTGCATTA     >  1:450725/1‑151
                                                                                                                                                               AACTTAATGTATTTAGAATGGAATTACTTGGTGCAAAGGTTGTGGCAGTTGAAGATGGTCAAGGGACTTTATCGGATGCAGTTAATAAAGCATTGCAATATTGGGTAAGTCATGTAGATGATACACATTATTTATTAGGTTCTGCATTAGG   <  2:706104/151‑1
                                                                                                                                                                ACTTAATGTATTTAGAATGGAATTACTTGGTGCAAAGGTTGTGGCAGTTGAAGATGGTCAAGGGACTTTATCGGATGCAGTTAATAAAGCATTGCAATATTGGGTAAGTCATGTAGATGATACACATTATTTATTAGGTTCTGCATTAGGT  <  1:792708/151‑1
                                                                                                                                                                                                                                                                                                                       
ATCATCTACATGACTTACCCAATATTGCAATGCTTTATTAACTGCATCCGATAAAGTCCCTTGACCATCTTCAACTGCCACAACCTTTGCACCAAGTAATTCCATTCTAAATACATTAAGTTGTTGTCTTTTAATATCTTCACTTCCCATAAAGACAACAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  CP000730/1409305‑1409145
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑aaagacaacaaCTTAATGTATTTAGAATGGAATTACTTGGTGCAAAGGTTGTGGCAGTTGAAGATGGTCAAGGGACTTTATCGGATGCAGTTAATAAAGCATTGCAATATTGGGTAAGTCATGTAGATGATACACATTATTTATTAGGTTCTGCATTAGGT  >  CP000730/1409186‑1409335

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.