New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	CP000730	1473650 =	45 (1.520)	4 (0.140)	4/286	1.2	9.0%	coding (28374/31266 nt)	ebh	extracellular matrix binding protein
	?	CP000730	1473682 =	38 (1.340)					coding (28342/31266 nt)	ebh	extracellular matrix binding protein
Rejected: Frequency below/above cutoff threshold.

ATTGCGCCATTGATATCATTCACATTTGTGTTATCACTATTTAGCAATGTATTTGCTAGACGTTTGGCATCATCGAAGTTTGTTTTAGCATTATCGTCAGCGTTTTGGTAATTGACAGTTTGCTCTGCATTTGGAATTTCATTGT‑CAACTAAATGTTTCAATGTTTCCATTGCATCATTTAAGTCAATTTGATTATTAACAATATCTGTTACATCTGATACAGTATCGGCATTGTTAATTGCTTTATGTGCAAGATCTTGTTGCTGTTGATTTAATCCATTTAACGAATTAACAAACGC  >  CP000730/1473501‑1473799                                                                                                                                                       |                                                                                                                                                      aTTGCGCCATTGATATCATTCACATTTGTGTTATCACTATTTAGCAATGTATTTGCTAGACGTTTGGCATCATCGAAGTTTGTTTTAGCATTATCGTCAGCGTTTTGGTAATTGACAGTTTGCTCTGCATTTGGAATTTCATTGT‑CAACTa                                                                                                                                                      <  2:281222/151‑1 (MQ=255)      gcCATTGATATCATTCACATTTGTGTTATCACTATTTAGCAATGTATTTGCTAGACGTTTGGCATCATCGAAGTTTGTTTTAGCATTATCGTCAGCGTTTTGGTAATTGACAGTTTGCTCTGCATTTGGAATTTCATTGT‑CAACTAaatgt                                                                                                                                                 <  2:321733/151‑1 (MQ=255)       ttgcaaatgccaaagataaagcaaatgcgtttgttaattcgttaaatggattaaatcaacagcaacaagatcttgcacataaagcaattaacaatgccgatactgtatcagatgtaacagatattgttaataatcaaattgactTAaatgt                                                                                                                                                 <  1:63346‑M1/7‑1 (MQ=255)           tGATATCATTCACATTTGTGTTATCACTATTTAGCAATGTATTTGCTAGACGTTTGGCATCATCGAAGTTTGTTTTAGCATTATCGTCAGCGTTTTGGTAATTGACAGTTTGCTCTGCATTTGGAATTTCATTGT‑CAACTAaatgtttcaa                                                                                                                                            <  2:378226/151‑1 (MQ=255)                tCATTCACATTTGTGTTATCACTATTTAGCAATGTATTTGCTAGACGTTTGGCATCATCGAAGTTTGTTTTAGCATTATCGTCAGCGTTTTGGTAATTGACAGTTTGCTCTGCATTTGGAATTTCATTGT‑CAACTAaatgtttcaatgttt                                                                                                                                       >  1:442576/1‑151 (MQ=255)                   ttCACATTTGTGTTATCACTATTTAGCAATGTATTTGCTAGACTTTTGGCATCATCGAAGTTTGTTTTAGCATTATCGTCAGCGTTTTGGTAATTGACAGTTTGCACAGCCTTTGG‑CTGCCACTGTGCAACTAAATGTTTCAATGTTTCCa                                                                                                                                    <  2:112274/151‑1 (MQ=255)                      acaTTTGTGTTATCACTATTTAGCAATGTATTTGCTAGACGTTTGGCATCATCGAAGTTTGTTTTAGCATTATCGTCAGCGTTTTGGTAATTGACAGTTTGCTCTGCATTTGGAATTTCATTGT‑CAACTAAATGTTTCAATGTTTCCATTg                                                                                                                                 <  2:152112/151‑1 (MQ=255)                            gtgtTATCACTATTTAGCAATGTATTTGCTAGACGTTTGGCATCATCGAAGTTTGTTTTAGCATTATCGTCAGCGTTTTGGTAATTGACAGTTTGCTCTGCATTTGGAATTTCATTGT‑CAACTAAATGTTTCAATGTTTCCATTGcatcat                                                                                                                           >  1:226150/1‑151 (MQ=255)                            gtgtTATCACTATTTAGCAATGTATTTGCTAGACGTTTGGCATCATCGAAGTTTGTTTTAGCATTATCGTCAGCGTTTTGGTAATTGACAGTTTGCTCTGCATTTGGAATTTCATTGT‑CAACTAAATGTTTCAATGTTTCCATTGcatcat                                                                                                                           >  1:310668/1‑151 (MQ=255)                            gtgtTATCACTATTTAGCAATGTATTTGCTAGACGTTTGGCATCATCGAAGTTTGTTTTAGCATTATCGTCAGCGTTTTGGTAATTGACAGTTTGCTCTGCATTTGGAATTTCATTGT‑CAACTAAATGTTTCAATGTTTCCATTGcatcat                                                                                                                           >  2:187822/1‑151 (MQ=255)                              gtTATCACTATTTAGCAATGTATTTGCTAGACGTTTGGCATCATCGAAGTTTGTTTTAGCATTATCGTCAGCGTTTTGGTAATTGACAGTTTGCTCTGCATTTGGAATTTCATTGT‑CAACTAAATGTTTCAATGTTTCCATTGCATCAttt                                                                                                                         >  1:134842/1‑151 (MQ=255)                               ttATCACTATTTAGCAATGTATTTGCTAGACGTTTGGCATCATCGAAGTTTGTTTTAGCATTATCGTCAGCGTTTTGGTAATTGACAGTTTGCTCTGCATTTGGAATTTCATTGT‑CAACTAAATGTTTCAATGTTTCCATTGCATCATTTa                                                                                                                        <  2:32062/151‑1 (MQ=255)                               ttATCACTATTTAGCAATGTATCTGCTAGACGTTTGGCATCATCGAAGTTTGTTTTAGCATTATCGTCAGCGTTTTGGTAATTGACAGTTTGCTCTGCATTTGGAATTTCATCGT‑CAACTAAATGTTTCAATGTTTCCATTGCATCAtttn                                                                                                                        <  2:216130/151‑2 (MQ=255)                                tgcgtttgttaattcgttaaatggattaaatcaacagcaacaagatcttgcacataaagcaattaacaatgccgatactgtatcagatgtaacagatattgttaataatcaaattgactTAAATGTTTCAATGTTTCCATTGCATCATTTa                                                                                                                        >  2:108904‑M1/120‑151 (MQ=255)                                 aTCACTATTTAGCAATGTATTTGCTAGACGTTTGGCATCATCGAAGTTTGTTTTAGCATTATCGTCAGCGTTTTGGTAATTGACAGTTTGCTCTGCATTTGGAATTTCATTGT‑CAACTAAATGTTTCAATGTTTCCATTGCATCATTTAAg                                                                                                                      <  1:450014/151‑1 (MQ=255)                                 aTCACTATTTAGCAATGTATTTGCTAGACGTTTGGCATCATCGAAGTTTGTTTTAGCATTATCGTCAGCGTTTTGGTAATTGACAGTTTGCTCTGCATTTGGAATTTCATTGT‑CAACTAAATGTTTCAATGTTTCCATTGCATCATTTAAg                                                                                                                      <  1:5512/151‑1 (MQ=255)                                 aTCACTATTTAGCAATGTATTTGCTAGACGTTTGGCATCATCGAAGTTTGTTTTAGCATTATCGTCAGCGTTTTGGTAATTGACAGTTTGCTCTGCATTTGGAATTTCATTGT‑CAACTAAATGTTTCAATGTTTCCATTGCATCATTTAAg                                                                                                                      <  1:240542/151‑1 (MQ=255)                                 aTCACTATTTAGCAATGTATTTGCTAGACGTTTGGCATCATCGAAGTTTGTTTTAGCATTATCGTCAGCGTTTTGGTAATTGACAGTTTGCTCTGCATTTGGAATTTCATTGT‑CAACTAAATGTTTCAATGTTTCCATTGCATCATTTAAg                                                                                                                      <  2:96975/151‑1 (MQ=255)                                   cACTATTTAGCAATGTATTTGCTAGACGTTTGGCATCATCGAAGTTTGTTTTAGCATTATCGTCAGCGTTTTGGTAATTGACAGTTTGCTCTGCATTTGGAATTTCATTGT‑CAACTAAATGTTTCAATGTTTCCATTGCATCATTTAAGTc                                                                                                                    <  2:221890/151‑1 (MQ=255)                                            gCAATGTATTTGCTAGACGTTTGGCATCATCGAAGTTTGTTTTAGCATTATCGTCAGCGTTTTGGTAATTGACAGTTTGCTCTGCATTTGGAATTTCATTGT‑CAACTAAATGTTTCAATGTTTCCATTGCATCATTTAAGTCAATTTGatt                                                                                                           >  1:105038/1‑151 (MQ=255)                                             cAATGTATTTGCTAGACGTTTGGCATCATCGAAGTTTGTTTTAGCATTATCGTCAGCGTTTTGGTAATTGACAGTTTGCTCTGCATTTGGAATTTCATTGT‑CAACTAAATGTTTCAATGTTTCCATTGCATCATTTAAGTCAATTTGatta                                                                                                          <  2:300401/151‑1 (MQ=255)                                              aaTGTATTTGCTAGACGTTTGGCATCATCGAAGTTTGTTTTAGCATTATCGTCAGCGTTTTGGTAATTGACAGTTTGCTCTGCATTTGGAATTTCATTGT‑CAACTAAATGTTTCAATGTTTCCATTGCATCATTTAAGTCAATTTGattat                                                                                                         <  2:399875/151‑1 (MQ=255)                                              aaTGTATTTGCTAGACGTTTGGCATCATCGAAGTTTGTTTTAGCATTATCGTCAGCGTTTTGGTAATTGACAGTTTGCTCTGCATTTGGAATTTCATTGT‑CAACTAAATGTTTCAATGTTTCCATTGCATCATTTAAGTCAATTTGattat                                                                                                         <  2:235363/151‑1 (MQ=255)                                              aaTGTATTTGCTAGACGTTTGGCATCATCGAAGTTTGTTTTAGCATTATCGTCAGCGTTTTGGTAATTGACAGTTTGCTCTGCATTTGGAATTTCATTGT‑CAACTAAATGTTTCAATGTTTCCATTGCATCATTTAAGTCAATTTGattat                                                                                                         <  2:291317/151‑1 (MQ=255)                                                       gCTAGACGTTTGGCATCATCGAAGTTTGTTTTAGCATTATCGTCAGCGTTTTGGTAATTGACAGTTTGCTCTGCATTTGGAATTTCATTGT‑CAACTAAATGTTTCAATGTTTCCATTGCATCATTTAAGTCAATTTGATTATTAACAatat                                                                                                >  2:115884/1‑151 (MQ=255)                                                          aGACGTTTGGCATCATCGAAGTTTGTTTTAGCATTATCGTCAGCGTTTTGGTAATTGACAGTTTGCTCTGCATTTGGAATTTCATTGT‑CAACTAAATGTTTCAATGTTTCCATTGCATCATTTAAGTCAATTTGATTATTAACAATATCTg                                                                                             <  2:160906/151‑1 (MQ=255)                                                           gACGTTTGGCATCATCGAAGTTTGTTTTAGCATTATCGTCAGCGTTTTGGTAATTGACAGTTTGCTCTGCATTTGGAATTTCATTGT‑CAACTAAATGTTTCAATGTTTCCATTGCATCATTTAAGTCAATTTGATTATTAACAATATCTGt                                                                                            >  2:430544/1‑151 (MQ=255)                                                            aCGTTTGGCATCATCGAAGTTTGTTTTAGCATTATCGTCAGCGTTTTGGTAATTGACAGTTTGCTCTGCATTTGGAATTTCATTGT‑CAACTAAATGTTTCAATGTTTCCATTGCATCATTTAAGTCAATTTGATTATTAACAATATCTGtt                                                                                           <  1:183121/151‑1 (MQ=255)                                                            aCGTTTGGCATCATCGAAGTTTGTTTTAGCATTATCGTCAGCGTTTTGGTAATTGACAGTTTGCTCTGCATTTGGAATTTCATTGT‑CAACTAAATGTTTCAATGTTTCCATTGCATCATTTAAGTCAATTTGATTATTAACAATATCTGtt                                                                                           <  1:146948/151‑1 (MQ=255)                                                             cGTTTGGCATCATCGAAGTTTGTTTTAGCATTATCGTCAGCGTTTTGGTAATTGACAGTTTGCTCTGCATTTGGAATTTCATTGT‑CAACTAAATGTTTCAATGTTTCCATTGCATCATTTAAGTCAATTTGATTATTAACAATATCTGTTa                                                                                          >  1:241443/1‑151 (MQ=255)                                                                        atcGAAGCTTGTTTTAGCATTATCGTCAGCGTTTTGGTAATTGACAGTTTGCTCTGCATTTTGAATTTCCTTGT‑CAACTAAATGTTTCGATGTTTCCATTGCATCATTTAAGTAAATTTGATTATTAACAATATCTTTTACATCTTATAct                                                                               >  2:66395/1‑150 (MQ=255)                                                                         tcGAAGTTTGTTTTAGCATTATCGTCAGCGCTTTGGTAATTGACAGTTTGCTCTGCATTTGGAATTTCATTGT‑CAACTAAATGTTTCAATGTTTCCATTGCATCATTTAAGTCAATTTGATTATTAACAATATCTGTTACATCTGATACAg                                                                              >  2:419802/1‑151 (MQ=255)                                                                                  gtttTAGCATTATCGTCAGCGTTTTGGTAATTGACAGTTTGCTCTGCATTTGGAATTTCATTGT‑CAACTAAATGTTTCAATGTTTCCATTGCATCATTTAAGTCAATTTGATTATTAACAATATCTGTTACATCTGATACAGTATCGGCAt                                                                     >  1:353820/1‑151 (MQ=255)                                                                                    tttAGCATTATCGTCAGCGTTTTGGTAATTGACAGTTTGCTCTGCATTTGGAATTTCATTGT‑CAACTAAATGTTTCAATGTTTCCATTGCATCATTTAAGTCAATTTGATTATTAACAATATCTGTTACATCTGATACAGTATCGGCATTg                                                                   <  2:399087/151‑1 (MQ=255)                                                                                               cGTCAGCGTTTTGGTAATTGACAGTTTGCTCTGCATTTGGAATTTCATTGT‑CAACTAAATGTTTCAATGTTTCCATTGCATCATTTAAGTCAATTTGATTATTAACAATATCTGTTACATCTGATACAGTATCGGCATTGTTAATTGCttt                                                        <  2:150286/151‑1 (MQ=255)                                                                                                gTCAGCGTTTTGGTAATTGACAGTTTGCTCTGCATTTGGAATTTCATTGT‑CAACTAAATGTTTCAATGTTTCCATTGCATCATTTAAGTCAATTTGATTATTAACAATATCTGTTACATCTGATACAGTATCGGCATTGTTAATTGCTTTa                                                       <  1:77064/151‑1 (MQ=255)                                                                                                  cAGCGTTTTGGTAATTGACAGTTTGCTCTGCATTTGGAATTTCATTGT‑CAACTAAATGTTTCAATGTTTCCATTGCATCATTTAAGTCAATTTGATTATTAACAATATCTGTTACATCTGATACAGTATCGGCATTGTTAATTGCTTTAtg                                                     >  2:223268/1‑151 (MQ=255)                                                                                                  cAGCGTTTTGGTAATTGACAGTTTGCTCTGCATTTGGAATTTCATTGT‑CAACTAAATGTTTCAATGTTTCCATTGCATCATTTAAGTCAATTTGATTATTAACAATATCTGTTACATCTGATACAGTATCCGCATTGTTAATTGAttgttg                                                     >  2:332280/1‑147 (MQ=255)                                                                                                          tGGTAATTGACAGTTTGCTCTGCATTTGGAATTTCATTGT‑CAACTAAATGTTTCAATGTTTCCATTGCATCATTTAAGTCAATTTGATTATTAACAATATCTGTTACATCTGATACAGTATCGGCATTGTTAATTGCTTTATGTGCAAGAt                                             <  2:268237/151‑1 (MQ=255)                                                                                                               aTTGACAGTTTGCTCTGCATTTGTAATTTCATTGT‑CAACTAAATGTTTCAATGTTTCCATTGCATCATTTAAGTCAATTTGATTATTAACAATATCTGTTACATCTGATACAGTATCGGCATTGTTAATTGCTTTATGTGCAAGATCttgt                                        <  1:115884/151‑1 (MQ=255)                                                                                                                     aGTTTGCTCTGCATTTGGAATTTCATTGT‑CAACTAAATGTTTCAATGTTTCCATTGCATCATTTAAGTCAATTTGATTATTAACAATATCTGTTACATCTGATACAGTATCGGCATTGTTAATTGCTTTATGTGCAAGATCTTGTTGCTGt                                  <  1:430544/151‑1 (MQ=255)                                                                                                                               gCATTTGGAATTTCATTGT‑CAACTAAATGTTTCAATGTTTCCATTGCATCATTTAAGTCAATTTGATTATTAACAATATCTGTTACATCTGATACAGTATCGGCATTGTTAATTGCTTTATGTGCAAGATCTTGTTGCTGTTGACTTAATc                        <  2:371832/151‑1 (MQ=255)                                                                                                                                    tGGAATTTCATTGT‑CAACTAAATGTTTCAATGTTTCCATTGCATCATTTAAGTTAATTTGATTATTAACAATATCTGTTACATCTGATACAGTATCGGCATTGTTAATTGCTTTATGTGCAAGATCTTGTTGCTGTTGATTTAATCCAttt                   >  1:342573/1‑151 (MQ=255)                                                                                                                                    gttaataatcaaattgactTAAATGATGCAATGGAAACATTGAAACATTTAAGTCAATTTGATTATTAACAATATCTGTTACATCTGATACAGTATCGGCATTGTTAATTGCTTTATGTGCAAGATCTTGTTGCTGTTGATTTAATCCAtt                    <  1:86724‑M1/132‑1 (MQ=255)                                                                                                                                       aaTTTCATTGT‑CAACTAAATGTTTCAATGTTTCCATTGCATCATTTAAGTCAATTTGATTATTAACAATATCTGTTACATCTGATACAGTATCGGCATTGTTAATTGCTTTATGTGCAAGATCTTGTTGCTGTTGATTTAATCCATTTAAc                <  1:26220/151‑1 (MQ=255)                                                                                                                                        ataatcaaattgactTAAATGATGCAATGGAAACATTGAAACATTTAAGTCAATTTGATTATTAACAATATCTGTTACATCTGATACAGTATCGGCATTGTTAATTGCTTTATGTGCAAGATCTTGTTGCTGTTGATTTAATCCATTTAAc                <  2:63346‑M1/136‑1 (MQ=255)                                                                                                                                              ttGT‑CAACTAAATGTGTGAATGTTTCCATTGCATCATTTAAGTCAATTTGATTATTAACAATATCTTTTACATTTGCTACAGTATCGGCATTGTTAATTGCTTTATGGGCAAGATCTTGTTGCTGTTGATTTAATCCATTTAACGAATTaa         <  2:327090/151‑1 (MQ=255)                                                                                                                                                    aaCTAAATGTTTCAATGTTTCCATTGCATCATTTAAGTCAATTTGATTATTAACAATATCTGTTACATCTGATACAGTATCAGCATTGTTAATTGCTTTATGTGCAAGATCTTGTTGCTGTTGATTTAATCCATTTAACGAATTAACAAAc    >  1:295212/1‑151 (MQ=255)                                                                                                                                                      cTAAATGTTTCAATGTTTCCATTGCATCATTTAAGTCAATTTGATTATTAACAATATCTGTTACATCTGATACAGTATCGGCATTGTTAATTGCGGTATGTGCAAGATCTTGTTGCTGTTGATTTAATCCATTTAACGAATTAACAAACGc  >  1:87932/1‑151 (MQ=255)                                                                                                                                                       |                                                                                                                                                      ATTGCGCCATTGATATCATTCACATTTGTGTTATCACTATTTAGCAATGTATTTGCTAGACGTTTGGCATCATCGAAGTTTGTTTTAGCATTATCGTCAGCGTTTTGGTAATTGACAGTTTGCTCTGCATTTGGAATTTCATTGT‑CAACTAAATGTTTCAATGTTTCCATTGCATCATTTAAGTCAATTTGATTATTAACAATATCTGTTACATCTGATACAGTATCGGCATTGTTAATTGCTTTATGTGCAAGATCTTGTTGCTGTTGATTTAATCCATTTAACGAATTAACAAACGC  >  CP000730/1473501‑1473799

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.