Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC JC	NC_000913	1,757,115	IS1 (+) +9 bp	8.5%	intergenic (+5/‑298)	pykF → / → lpp	pyruvate kinase I/murein lipoprotein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 258675	NA (NA)	3 (0.040)	3/542	NT	6.3%	noncoding (1/768 nt)	IS1	repeat region
	?	NC_000913	= 1757123	43 (0.590)					intergenic (+13/‑298)	pykF/lpp	pyruvate kinase I/murein lipoprotein
*	?	NC_000913	1757115 =	43 (0.590)	5 (0.070)	5/542	NT	10.1%	intergenic (+5/‑306)	pykF/lpp	pyruvate kinase I/murein lipoprotein
	?	NC_000913	1978503 =	NA (NA)					noncoding (768/768 nt)	IS1	repeat region

TGATCGTGGTTGCTACTCAGGG‑CGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGAAGCAATAAAAAATCAAATCGGATTTCACTATATAATCTCACTTTATCTAAGATGAATCCGATGGAAGCATCCTGTTTTCTCTCAATTTTTTTATCTAAAACCCAGCGTTCGATGCTTCTTTGAGCGAACGATCAAAAATAAGTGCCTTCCCATCAAAAAAATATTCTCAACATAAAAAACTTTGTGTAATACTTGTAACGCTACATGGAGATTAACTCAATCTAGAGGGTAT  >  NC_000913/1756815‑1757415                                                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                                                             tggtagttgTTGCTACACAGGGACGGTAAA‑CGGAACGGCCAGTACGGAACGACGTCCCGGATGGCATCATACGGGCACTGACCACCAACGAAAAATTGGCTAAGCAGTTGGTACTGAGCAAAGGTGTTGTGCTGCAGCTTGTTAAAGAGATCACTTGTACTGATGAGTTCTACCGTCTGGGTAAAGAACTGGCTGTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTg                                                                                                                                                                                                                                                                                                              <  2:210832/293‑1 (MQ=255) tGATCGTGGTGGCTACTCAGGG‑CGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTg                                                                                                                                                                                                                                                                                                              <  1:550437/301‑1 (MQ=255)        ggTTGCTACTCAGGG‑CGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAACGGTGCCTTTGTAGTTATGGTTTCTGGTGCACTGGTACCGACCGGCCCTACTACCCCCGCCTCTGTTCCCGTCCTGTAATATTGCtttttg                                                                                                                                                                                                                                                                                                       >  2:618912/1‑299 (MQ=255)          ttGCTACTCAGGG‑CGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAACGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTATCGTATTGGTTTCTGGTTGACTGGTGCCGAGAGGCCCTACTACAACCGCATCTGTTTCCGTCCTGTACTCTGTCTTTtgtag                                                                                                                                                                                                                                                                                                     >  2:373652/1‑299 (MQ=255)          ttGCTACTCAGGG‑CGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCCCTTCTACTGATGATTTCTACCGTCTGTGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACTTTGTAGTTCTGTTTTCTGCTGCACTGCTACCGAGCGGACCTACTAACCACGACTCTTTTCACGTCCTGTAATATTGCTTTtgtta                                                                                                                                                                                                                                                                                                     >  1:173864/1‑299 (MQ=255)          tggCTACTTAGGG‑CGGTAAAGCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTtgtg                                                                                                                                                                                                                                                                                                      <  2:675743/298‑1 (MQ=255)             cTACTCAGGG‑CGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGTCACTACTAACACCGCATCTGTTCCCGTCCTGTAATATTGCTTTTGTGaatt                                                                                                                                                                                                                                                                                                  >  1:35060/1‑301 (MQ=255)                                             aCGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGCGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTCTAATATTCCTTTTGTCACTTAATTTGTATTTCTAAACGCCCTGTTGGgcg                                                                                                                                                                                                                                                                    >  2:694319/1‑300 (MQ=255)                                             aCGTAAATACTTCCCGGATGCAACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTGCTGAGCAAAGGCGTTGTGCCGACGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAGAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAGGGTGACGTTGTAGTTATGGTTTCTTGTGCACTGGACCCGAGTGGCAATACTAACACTGCATCTGTTCACTACATGAAATATTGCTTCTGTGCTTTAATTTTTATATCTATGCCCCTTGATggtagg                                                                                                                                                                                                                                                                   >  2:22549/1‑297 (MQ=255)                                             aCGGAAATACTGCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGgcgc                                                                                                                                                                                                                                                                   <  2:35058/301‑1 (MQ=255)                                                tAAATACTTCCCGGATGCCACCATCCTGGAACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGATATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGggtgatgctgccaacttactgatttagtgtatgatgg                                                                                                                                                                                                                                                                 >  1:618936‑M2/1‑263 (MQ=255)                                                        ccctttgacgtggtgatatggatgacggatggctggccgctgtatgaatcccgcctgaagggaaagctgcacgtaatcagcaagcgatatacgcagcgaattgagcggcataacctgaatctgaggcagcacctggcacggctgggacggaagtcgctgtcgttctcaaaatcggtggagctgcatgacaaagtcatcgggcattatctgaacataaaacactatcaataagttggagtcattaccGCTTTTGTGAATTCATTTGTATATCGAAGCGCCCTGAGTGGCGATTTTtttattt                                                                                                                                                                                                                                                        <  2:287732‑M1/55‑1 (MQ=255)                                                             gATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACCAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCATGTAATATTGCTTTTTTGAATTAATTTGTCTCTCTACGCGCCCTCATGTGCGCTTTTTTTTTTTAATCg                                                                                                                                                                                                                                                   >  2:508946/1‑301 (MQ=255)                                                                 ccaccaTCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGAATTAATTTTTATATCGAAGCGCCCTGATGGGCGCTTTTTTTTTTTAATCTATaa                                                                                                                                                                                                                                               >  2:528086/1‑301 (MQ=255)                                                                   accaTTCTTGCAATGAACACCAACGAGAAAACGTATCATCAGTGGGTACGTAGCGAAGGCGTTGTGACGCAGCGTGTTGAAGAGAGCGGTTCTACTGGTGATTTCTACCGGTGGGGTAAAGAACAGGCGCTGCAGAGCGGTATGGCACACAAAGGTGACGGTGTAGTTAGTGTTTCTGGTGCACTGCTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTGTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAAcc                                                                                                                                                                                                                                             <  2:394872/301‑1 (MQ=255)                                                                       tCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTGAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGCATTAATTTGTTAATCGAAGCGCCCTGATCGGCGCTTTTTTTATTTAATCGATAACCAGaa                                                                                                                                                                                                                                         >  2:227218/1‑301 (MQ=255)                                                                                                    gCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCTATATCCAGAAGCAATCAAAAATCAAATCGATTTTAACta                                                                                                                                                                                                            >  2:718549/1‑301 (MQ=255)                                                                                                    gCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGTTAACCAGAAGCAATAAAAAATCAAATCGGATTTCACta                                                                                                                                                                                                            >  2:284600/1‑301 (MQ=255)                                                                                                       catcaGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATGGCTTTTGTGCATTAATTTGTATATCGAAGCGCCCTGATGAGCGCTTTTTTTATTTAATCGATAACCAGAAGc                                                                                                                                                                                                                                       >  2:636883/1‑271 (MQ=255)                                                                                                         tcaGGTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGggtgatgctgccaacttactgatttagtgtatgatggtgtttttgaggtgctccagtggcttctgtttctatcagctgtccctcctgttcagcta                                                                                                                                                                                                       >  1:102676‑M2/1‑206 (MQ=255)                                                                                                             ttGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTGCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGAAGCAATAAAAAATCAAATCGGATTTCACTATATAAtc                                                                                                                                                                                                     <  1:528109/299‑1 (MQ=255)                                                                                                                 aagggaaagctgaacgtaagcagcaagcgatatacgcagcgaattgagcggcataacctgaatctgaggcagcacctggcacggctgggacggaagtcgctgtcgttctcaaaatcggtggagctgcatgacaaagtcatcgggcattatctgaacataaaacactatcaataagttggagtcattaccGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGAAGCAATAAAAAATCAAATCGGATTTCACTATATAATCTCACtt                                                                                                                                                                                               >  1:287738‑M1/190‑301 (MQ=255)                                                                                                                             gCGTTGTGCCGCAGCTTGTTAAAGAGATCACGTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGAGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGAAGCAATAAAAAATCAAATCGGATTTCACTATATAATCTCACTTTATCTAAGAt                                                                                                                                                                                     <  1:520456/299‑1 (MQ=255)                                                                                                                                                        taccTTCTACTGATTATTTGTACCGGCAGGTGAAAGAACTTGCTCGTGAGAGCGGCCTGGCATACAAAGGTGACGTTGTAGTTATGGTTTCGGGTACACTGGTACCTAGCGGCAATACTAACACCGCATCTGTTCACGTCCTGTAATATTTCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGAAGCAATAAAAAATCAAATCGGATTTCACTATATAATCTCACTTTATCTAAGATGAATCCGATGGAAGCATCCTGTTTtctct                                                                                                                                                        <  1:374660/298‑1 (MQ=255)                                                                                                                                                         cACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAACGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCCCTTTTTTTATTTAATTGATAACCAGAAGAAATAAAAAATCAAATCGGATTTCACTATATAATCCCACTTTATCTAAGATGAATCCGATGGAAGCCTCTTTTTTtcact                                                                                                                                                        >  2:382861/1‑300 (MQ=255)                                                                                                                                                           cTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTACTATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAAcc                                                                                                                                                                                                                                             >  1:486513/1‑213 (MQ=255)                                                                                                                                                                      gatTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGATTTTGTGAATTAATTTGTATGTCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAAGCGATAACAAGAAGAAATAGAAAATCAAATCGGATTTCACTATATAATCTCACTTTATCTAAGATGCATCCGATGGAAGCATCCTGTTTTCTCTCACTTTTTTTAt                                                                                                                                            >  1:643180/1‑299 (MQ=255)                                                                                                                                                                       atTTCTACCGGCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACGACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGggtgatgctgccaacttactgagttagtgtatgatggtgtttttgaggtgctccagtggcttctgtttctatcagctgtccctcctgttcagctactgacggggtggtgcgtaacggcaaaagcaccgccggacatcagcgctatctctgctctcac                                                                                                                                         >  1:343500‑M2/1‑144 (MQ=255)                                                                                                                                                                        tttCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGGTATGGTTTATGGTGCACTGGTACCGAGCGGCACGCCTAACACGGAACCTGTTCACGTCCTGTAATATTGCTTATGTGAATTAATTGGTATATAGACGCGCACTGATGGGCGCTTTTTTTATTAAATAGATCACCAACAGACATAAAAAATCTAATCGGATTTTCCTCAATTATGACACTTTCTCTACGATCAATTCGATGGACGCCTGCTATTATCCTTAATTTTTTTTTTCTaa                                                                                                                                        >  1:409326/1‑301 (MQ=255)                                                                                                                                                                                                 gCTCTGCAGGGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGAAGCAATAAAAAATCAAATCGGATTTCACTATATAATCTCACTTTATCTAAGATGAATCCGATGGAAGCATCCTGTTTTCTCTCAATTTTTTTATCTAAAACCCAGCGTTCGATGCTTCTTTg                                                                                                                <  1:227224/300‑1 (MQ=255)                                                                                                                                                                                                     gtataagagaaagggCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGAGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGAAGCAATAAAAAATCAAATCGGATTTCACTATATAATCTCACTTTATCTAAGATGAATCCGATGGAAGCATCCTGTTTTCTCTCAATTTTTTTATCTAAAACCCAGCGTTCGATGCTTCTTTGAGCg                                                                                                            <  1:579068/287‑1 (MQ=255)                                                                                                                                                                                                              gTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGAAGCAATCAAAAATCAAATCGGATTTCACTATCTAATCTCACTTTATCTAAGATGAATCCGATGGAAGCATCCTGTTTTCTCTCAATTTTTTTATCTAAAACCCACCGTTCGATGCTTCTTTGATCGAACGTTCaca                                                                                                  >  2:552823/1‑299 (MQ=255)                                                                                                                                                                                                                  ggCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGAGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGAATTAATCTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGAAGCAATAAAAAATCAAATCGGATTTCACTATATAATCTCACTTTATCTAAGATGAATCCGATGGAAGCATCCTGTTTTCTCTCAATTTTTTTATCTACAACACAGCGTTCGATGCTTCtttccgcgctttctcttctac                                                                                               >  2:579045/1‑282 (MQ=255)                                                                                                                                                                                                                                aCGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCCTCTGTTCACGTCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTACTCGATAACCAGAAGCAATAACAAATCAAATCGGATTTCACTATATAATCTCACTTTATCTAAGATGAATCCGATAGAATCGTCCTGTTTTCTCTCAATTTTTTTATCTAAAAACCAGCGTTCGATGCTTCTTTGAGAGAACGATCAAAAATAAGTGCCTTACCAtt                                                                                >  1:605647/1‑300 (MQ=255)                                                                                                                                                                                                                                 cGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTCATCGATAACCAGAAGCAATAAAAAATCAAATCGGATTTCACTATATAATCTCACTTTATCTAAGATGAATCCGATGGAAGCATCCTTTTTTCTCTCAATTTTTTTATCTAAAACCCAGCGTTCGATTCTTCTTTGAGCGAACGTTCAAAAATAATTGCATTCCCAta                                                                                >  2:743121/1‑299 (MQ=255)                                                                                                                                                                                                                                            tGGTTTTCGGTGCACTGGTAACGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGAAGCAATAAAAAATCAAATCGGATTTCACTATATAATCTCACTTTATCTAAGATGAATCCGATGGAAGCATCCTGTTTTCTCTCAATTTTTTTATCTAAAACCCAGCGTTCGATGCTTCTTTGAGCGAACGATCAAAAATAAGTGCCTTCCCATCAAAAAAATATtc                                                                    <  1:284606/301‑1 (MQ=255)                                                                                                                                                                                                                                            tGGGTTCTGGTGCACTGGGAACGAGCGGCACTAATAATACCGCATCTTTTCACGTCCTGTAATATTGCTTTTGTGAATTCATTTGTATATCTAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGAAGCAATAAAAAATCAAATCGGATTTCACTATATAATCTCACTTTATCTAAGATGAATCCGATGGAAGCATCCTGTTTTCTCTCAATTTTTTTATCTAAAACCCAGCGTTCGATGCTTCTTTGAGCGAACGATCAAAAATAAGTGCCTTCCCATCAAAAAAATATtc                                                                    <  1:718575/301‑1 (MQ=255)                                                                                                                                                                                                                                             ggTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGACTTAATTTGTATATCGAAGCGCCCTGATGGgc                                                                                                                                                                                                                                                                     >  2:141844/1‑107 (MQ=255)                                                                                                                                                                                                                                             ggTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGgc                                                                                                                                                                                                                                                                     <  1:141850/107‑1 (MQ=255)                                                                                                                                                                                                                                               tgtgtggtttaCTGGGACCGAGCGGCACTACTAACACCGGATCTGTTCACGTCCTGTAATATTGCTTGTGTTAATTAATTTGTATATCGAAGAGCCCTGACGGGCGCTTGTTTTATTTAATCGATAACCAGAAGCAATAAAAAATCAAATCGGATTTCACTATATAATCTCACTTTATCTAAGATGAATCCGATGGAAGCATCCTGTTTTCTCTCAATTTTTTTATCTAAAACCCAGCGTTCGATGCTTCTTTGAGCGAACGATCAAAAATAAGTGCCTTCCCATCAAAAAAATATTCTCa                                                                 <  2:643154/291‑1 (MQ=255)                                                                                                                                                                                                                                                        cACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGAAGc                                                                                                                                                                                                                                       >  2:154246/1‑126 (MQ=255)                                                                                                                                                                                                                                                        cACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGAAGc                                                                                                                                                                                                                                       <  1:154253/126‑1 (MQ=255)                                                                                                                                                                                                                                                                      tgtactactAACACCGCATCTGTTCCAGTCCTGTAATCTTTCTTTTGGTAATTAATTTGTATATCGAAGCTCCCTGATGTGCGCTCTTTTTATTTTATCGATAACCAGAAGCCATAAAGAATCAAATCGGATTTCACTATATAATCTCACTTTATCCAAGATGAATCCGATGGAAGCATCCTGTTTTCTCTTAATTTTTTTATCTAACTCCCTGCGTTCGTTGCTTCTTTGAGCGAACGATCAAAAATAAGTGCCTTCCCATCAAAAAAATATTCTCACCATAAAAAACTTTGTGTAATAc                                          <  1:22551/298‑1 (MQ=255)                                                                                                                                                                                                                                                                      tatgaaaataaaaaactaataaaaaggtggagtcattaccGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATTGGCGCTTTTTTTATTTAATCGATAACCAGAAGCAATAAAAAATCAAATCGGATTTCACTATATAATCTCACTTTATCTAAGATGAATCCGATGGAAGCATCCTGTTTTCTCTCAATTTTTTTATCTAAAACCCAGCGTTCGATGCTTCTTTGAGCGAACGATCAAAAATAAGTGCCTTCCCATCAAAAAAATATTCTCAACATAAAAAACTTTGTGTAATAc                                          >  2:717101‑M1/41‑301 (MQ=255)                                                                                                                                                                                                                                                                       gCACTACTAAAACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGAAGCAATAAAAAATCAAATCGGATTTCACTATATAATCTCACTTTATCTAAGATGAATCCGATGGAAGCATCCTGTTTTCTCTCAATTTTTTTATCTAAAACCCAGCGTTCGATGCTTCTTTGAGCGAACGATCAAAAATAAGTGCCTTCCCATCAAAAAAATATTCTCAACATAAAAAACTTTGTGTAATAc                                          <  1:508969/300‑1 (MQ=255)                                                                                                                                                                                                                                                                       gCACTACTAAAAACGCATCTGTTCACGTCCTGTAATATTGCATTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTGATTTAATCGATAACCAGAAGCAATAAAAAATCAAATCGGATTTCACTATATAATCTCACTTTATCTAAGATGAATCCGATGGAAGCATCCTGTTTTCTCTCAATTTTTTTATCTAAAACCCAGCGTTCGATGCTTCTTTGAGCGAACGATCAAAAATAAGTGCCTTCCCATCAAAAAAATATTCTCAACATAAAAAACTTTGTGTAATAc                                          <  1:552846/300‑1 (MQ=255)                                                                                                                                                                                                                                                                       gCACTAATAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGAAGCAATAAAAAATCAAATCGGATTTCACTATATAATCTCACTTTATCTAAGATGAATCCGATGGAAGCATCCTGTTTTCTCTCAATTTTTTTATCTAAAACCCAGCGTTCGATGCTTCTTTGAGCGAACGATCAAAAATAAGTGCCTTCCCATCAAAAAAATATTCTCAACATAAAAAACTTTGTGTAATAc                                          <  2:409315/300‑1 (MQ=255)                                                                                                                                                                                                                                                                          ctactaACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGAAGCAATAAAAAATCAAATCGGATTTCACTATATAATCTCACTTTATCTAAGATGAATCCAATGGAAGCATCCTGTTTTCTCTCCATTTTTTTATCTAAAACCCATCTTTCGATGCTTCTTTGAGCGCACGATCAAAAATAAGTGCCTTCCCATCAAAAAAATATTCTCACCATAAAAAACTTTGTTTAATACTTGt                                      >  1:506907/1‑301 (MQ=255)                                                                                                                                                                                                                                                                           acataaaacactatcaataagttggagtcattaccGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGAAGCAATAAAAAATCAAATCGGATTTCACTATATAATCTCACTTTATCTAAGATGAATCCGATGGAAGCATCCTGTTTTCTCTCAATTTTTTTATCTAAAACCCAGCGTTCGATGCTTCTTTGAGCGAACGATCAAAAATAAGTGCCTTCCCATCAAAAAAATATTCTCAACATAAAAAAc                                                      <  1:131336‑M1/249‑1 (MQ=255)                                                                                                                                                                                                                                                                           acataaaacactatcaataagttggagtcattaccGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGAAGCAATAAAAAATCAAATCGGATTTCACTATATAATCTCACTTTATCTAAGATGAATCCGATGGAAGCATCCTGTTTTCTCTCAATTTTTTTATCTAAAACCCAGCGTTCGATGCTTCTTTGAGCGAACGATCAAAAATAAGTGCCTTCCCATCAAAAAAATATTCTCAACATAAAAAAc                                                      >  2:131330‑M1/36‑284 (MQ=255)                                                                                                                                                                                                                                                                             ctaACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGAAGCAATAAAAAATCAAATCGGATTTCACTATATAATCTCACTTTATCTAAGATGAATCCGATGAAAGCATCCTGTTTTCTTTCAATTTTTTTATCTAAAACACAGCGTTCGATGCTTCTTTTACAGAACGTGCAAAAATAAGTTCCTTCCCATCCACAAAATCTTCTCAACATAAAAAACATTTATTAATAATTTTaa                                    >  1:319363/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                           gTTCACGTCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGTCGCTTTTTTTATTTAATCGATAACCAGAAGCAATAAAAAATCAAATCGGATTTCGCTATATAATTTCACTTTATCTAAGATGAATCCGATGTAAGCATCCTGTCTTCTCTCAATTTTTTTATTTAAAACCCAGCGTTCGATGATTCTTTGAGCGAACGATACAAAATAAGTGCCTTCCCATACAAAAAATATTCTCAACATACAAAAATTCGTGTAATACTTGTACAGCTACATGGAGAtt                     >  1:610100/1‑301 (MQ=255)                                                                                                                                                                                                                                                                                              cACGTCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGAAGCAATAAAAAATCAAATCGGATTTCACTATATAATCTCACTTTATCTAAGATGAATCCGATGGAAGCATCCTGTTTTCTCTCAATTTTTTTATCTAAAACCCAGCGTTCGATGCTTCTTTGAGCGAACGATCAAAAATAAGTGCCTTCCCATCAAAAAAATATTCTCAACATAAAAAACTTTGTGTAATACTTGTAACGCTACATGGAGATTAAc                  <  1:382872/301‑1 (MQ=255)                                                                                                                                                                                                                                                                                                    cggTAATATGGCTTTTGTGAAATAATATGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGAAGCAATAAAAAATCAAATCGGATTTCACtatat                                                                                                                                                                                                         <  1:153358/110‑1 (MQ=255)                                                                                                                                                                                                                                                                                                    cTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGAAGCAATAAAAAATCAAATCTGGTTTCGATATGTAGTCGAACTTTATCTAAGGTGAGTCCTATGGAATCCTCCTGTTTtctctc                                                                                                                                                       >  1:112459/1‑162 (MQ=255)                                                                                                                                                                                                                                                                                                    cTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGAAGCAATAAAAAATCAAATCGGATTTCACtatat                                                                                                                                                                                                         >  2:153351/1‑112 (MQ=255)                                                                                                                                                                                                                                                                                                    cTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGAAGCAATAAAAAATCAAATCGGATTTCACTATATAATCTCACTTTATCTAAGATGAATCCGATGGAAGCATCCTGTTTtctctc                                                                                                                                                       <  2:112454/162‑1 (MQ=255)                                                                                                                                                                                                                                                                                                     tttAATATTGCTTTTGTGAATTAATTGGTAGATCGAACCTCCCTGATGGGCGCCTTTTTTATTTAATCGATAACCAGAAGCAATAAAAAATCAAATCGGATTTCACTATATAATCTCACTTTATCTAAGATGAATCCGATGGAAGCATCCTGTTTTCTCTCAATTTTTTTATCTAAAACCCAGCGTTCGATGCTTCTTTGAGCGAACGATCAAAAATAAGTGCCTTCCCATCAAAAAAATATTCTCAACATAAAAAACTTTGTGTAATACTTGTAACGCTACATGGAGATTAACTCAATCt           <  1:743148/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                                              gCTTTTGTGAATTACTTTGTATATCGAAGCGACCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGAAGCAATAAACAATCAAATCGGATTTCACTCTATAATCTCACTTTATCTAAGATGAATCCAATGGAAGCATCCTGTTTTCTCTCAATTTTTTTATCTAACACCCAGCGTTCGATTCTTCTTTTCGCGAACCGTCAAAAATAAGAGCCTTCACATCAAAAAAATCTTCTAACAATCAAACAATTTGTATACTACTTGTCAGTCTACATGGAGTTTAACTCAATCTAGAGGGTAt  >  2:110008/1‑301 (MQ=255)                                                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                                                             TGATCGTGGTTGCTACTCAGGG‑CGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGAAGCAATAAAAAATCAAATCGGATTTCACTATATAATCTCACTTTATCTAAGATGAATCCGATGGAAGCATCCTGTTTTCTCTCAATTTTTTTATCTAAAACCCAGCGTTCGATGCTTCTTTGAGCGAACGATCAAAAATAAGTGCCTTCCCATCAAAAAAATATTCTCAACATAAAAAACTTTGTGTAATACTTGTAACGCTACATGGAGATTAACTCAATCTAGAGGGTAT  >  NC_000913/1756815‑1757415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.