mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.27.1 revision 87c22d663cc3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| read file | reads | bases | passed filters | average | longest | mapped | |
|---|---|---|---|---|---|---|---|
| errors | SSW-KHP-SSW-1-163-8-1_S8_L001_R1_001 | 1,762,146 | 529,637,646 | 100.0% | 300.6 bases | 301 bases | 78.2% |
| errors | SSW-KHP-SSW-1-163-8-1_S8_L001_R2_001 | 1,759,801 | 528,441,306 | 99.9% | 300.3 bases | 301 bases | 75.9% |
| total | 3,521,947 | 1,058,078,952 | 99.9% | 300.4 bases | 301 bases | 77.0% |
| seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
|---|---|---|---|---|---|---|---|
| coverage | distribution | NC_000913 | 4,641,652 | 173.5 | 2.7 | 100.0% | Escherichia coli str. K-12 substr. MG1655, complete genome. |
| total | 4,641,652 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
| option | limit | actual |
|---|---|---|
| Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 19153 |
| Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 3 |
| Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 43 |
| Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.006 |
| reference sequence | pr(no read start) |
|---|---|
| NC_000913 | 0.79971 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
| option | value |
|---|---|
| Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
| Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 3 |
| Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 1 |
| option | value |
|---|---|
| Mode | Consensus/Mixed Base |
| Ploidy | 1 (haploid) |
| Consensus mutation E-value cutoff | 10 |
| Consensus frequency cutoff | 0.8 |
| Consensus minimum coverage each strand | OFF |
| Polymorphism E-value cutoff | 10 |
| Polymorphism frequency cutoff | 0.2 |
| Polymorphism minimum coverage each strand | OFF |
| Polymorphism bias cutoff | OFF |
| Predict indel polymorphisms | YES |
| Skip indel polymorphisms in homopolymers runs of | OFF |
| Skip base substitutions when they create a homopolymer flanked on each side by | OFF |
| step | start | end | elapsed |
|---|---|---|---|
| Read and reference sequence file input | 01:08:41 06 Mar 2016 | 01:10:35 06 Mar 2016 | 1 minute 54 seconds |
| Read alignment to reference genome | 01:10:36 06 Mar 2016 | 01:20:13 06 Mar 2016 | 9 minutes 37 seconds |
| Preprocessing alignments for candidate junction identification | 01:20:13 06 Mar 2016 | 01:21:58 06 Mar 2016 | 1 minute 45 seconds |
| Preliminary analysis of coverage distribution | 01:21:58 06 Mar 2016 | 01:26:16 06 Mar 2016 | 4 minutes 18 seconds |
| Identifying junction candidates | 01:26:16 06 Mar 2016 | 01:26:21 06 Mar 2016 | 5 seconds |
| Re-alignment to junction candidates | 01:26:21 06 Mar 2016 | 01:27:14 06 Mar 2016 | 53 seconds |
| Resolving alignments with junction candidates | 01:27:14 06 Mar 2016 | 01:31:52 06 Mar 2016 | 4 minutes 38 seconds |
| Creating BAM files | 01:31:52 06 Mar 2016 | 01:34:29 06 Mar 2016 | 2 minutes 37 seconds |
| Tabulating error counts | 01:34:29 06 Mar 2016 | 01:39:05 06 Mar 2016 | 4 minutes 36 seconds |
| Re-calibrating base error rates | 01:39:05 06 Mar 2016 | 01:39:06 06 Mar 2016 | 1 second |
| Examining read alignment evidence | 01:39:06 06 Mar 2016 | 02:11:47 06 Mar 2016 | 32 minutes 41 seconds |
| Polymorphism statistics | 02:11:47 06 Mar 2016 | 02:11:47 06 Mar 2016 | 0 seconds |
| Output | 02:11:47 06 Mar 2016 | 02:12:44 06 Mar 2016 | 57 seconds |
| Total | 1 hour 4 minutes 2 seconds | ||
