New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 157238068 (1.140)4 (0.070) 4/558 8.2 5.7% intergenic (‑335/+27) gadB/pqqL glutamate decarboxylase B, PLP‑dependent/putative periplasmic M16 family zinc metalloendopeptidase
?NC_000913 = 1572395 65 (1.110)intergenic (‑350/+12) gadB/pqqL glutamate decarboxylase B, PLP‑dependent/putative periplasmic M16 family zinc metalloendopeptidase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below cutoff threshold.

AAAAGTAGGATTTATCGATAAAGTAAGCAAGTGCAAAGGACTCGTGTTTAAATAACAAAATCCTAATGTTATTTATCGTGAGATATTACGCGAATAATATTTTTTCATTGAAAAACAATACAATATGAAATTCTTGGGTGGTGGTAAGGTGTTTTATGCTGTTATTTTTATGCGCATTCTGTGTCTCCTGAATTATCACGTAAAAATCAGACCTTAAAATATCACTATTAGTACTTGATTATTATTTTGAACGCATTTATAAAATTATTACATAAAAATAGCGAATATTGCTAAAATCCCCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/1572079‑1572380
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ccccgTCAACACATCGTTGGCGGGGATTTTAGCAATATTCGCTATTTTTATGTAATAATTTTATAAATGCGTTCAAAATAATAATCAAGTACTAATAGTGATATTTTAAGGTCTGATTTTTACGTGATAATTCAGGAGACACAGAATGCGCATAAAAATAACAGCATAAAACACCTTACCACCACCCAAGAATTTCATATTGTATTGTTTTTCAATGAAAAAATATTATTCGCGTAATATCTCACGATAAATAACATTAGGATTTTGTTAT  <  NC_000913/1572395‑1572130
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
AAAAGTAGGATTTATCGATAAAGTAAGCAAGTGCAAAGGACTCGTGTTTAAATAACAAAATCCTAATGTTATTTATCGTGAGATATTACGCGAATAATATTTTTTCATTGAAAAACAATACAATATGAAATTCTTGGGTGGTGGTAAGGTGTTTTATGCTGTTATTTTTATGCGCATTCTGTGTCTCCTGAATTATCACGTAAAAATCAGACCTTAAAATATCACTATTAGTACTTGATTATTATTTTGAACGCATTTATAAAATTATTACATAAAAATAGCGAATATTTCTAAAATCCCC                                                                                                                                                                                                                                                                             >  3:123984/1‑301
                                                                      ATTTATCGTGAGATATTACGCGAATAATATTTTTTCATTGAAAAACAATACAATATGAAATTCTTGGGTGGTGGTAAGGTGTTTTATGCTGTTATTTTTATGCGCATTCTGTGTCTCCTGAATTATCACGTAAAAATCAGACCTTAAAATATCACTATTAGTACTTGATTATTATTTTGAACGCATTTATAAAATTATTACATAAAAATAGCGAATATTGCTAAAATCCCCGTCAACACATCGTTGGCGGGGATTTTAGCAATATTAGCTATTTTTATGTAATAATTTTATAAATGCGTTt                                                                                                                                                                                                       >  1:334280/1‑300
                                                                                                           tcagatctCAATACAATATGAAATTCTTGGGTGGTGGTAAGGTGTTTTATGCTGTTATTTTTATGCGCATTCTGTGTCTCCTGAATTATCACGTAAAAATCAGACCTTAAAATATCACTATTAGTACTTGATTATTATTTTGAACGCATTTATAAAATTATTACATAAAAATAGCGAATATTGCTAAAATCCCCGTCAACACATCGTTGGCGGGGATTTTAGCAATATTCGCTATTTTTATGTAATAATTTTATAAATGCGTTCAAAATAATAATCAAGTACTAATAGTGATATTTTAAGG                                                                                                                                                                  <  2:15979/293‑1
                                                                                                                   CAATACAATATGAAATTCTTGGGTGGTGGTAAGGTGTTTTATGCTGTTATTTTTATGCGCATTCTGTGTCTCCTGAATTATCACGTAAAAATCAGACCTTAAAATATCACTATTAGTACTTGATTATTATTTTGAACGCATTTATAAAATTATTACATAAAAATAGCGAATATTGCTAAAATCCCCGTCAACACATCGTTGGCGGGGATTTTAGCAATATTCGCTATTTTTATGTAATAATTTTATAAATGCGTTCAAAATAATAATCAAGTACTAATAGTGATATTTTAAGGagatcgga                                                                                                                                                          >  4:15996/1‑293
                                                                                                                        aaagaaGAAAGTCTGTGGTGGTGGTAAGATGTTTTATGCTATTAGTATTATGCGCATTCTGTGTCTCCTGAATTATCACATAAGAATCAGACCGTAAAATATCACTATTAGTTCTTGATTATTATGTTGAACGCATTTAAAAAATTATTACATAAAAATAACGAATATAGCTAAAATCCCCATCAACATATCGTTGGCGGATATTTTAGCAATATTCGCTATTTTTATGTAATAATTTTATAAATGCGTTCAAAATAATAATCAAGTACTAATAGTGATATTTTAAGGTCTGATTTTTACG                                                                                                                                                     <  3:334267/295‑1
                                                                                                                                                                                                                                                                                                          CCCGTCAACACATCGTTGGCGGGGATTTTAGCAATATTCGCTATTTTTATGTAATAATTTTATAAATGCGTTCAAAATAATAATCAAGTACTAATAGTGATATTTTAAGGTCTGATTTTTACGTGATAATTCAGGAGACACAGAATGCGCATAAAAATAACAGCATAAAACACCTTACCACCACCCAAGAATTTCATATTGTATTGTTTTTCAATGAAAAAATATTATTCGCGTAATATCTCACGATAACTAACATTAGGATTTTGTTAT  >  3:303025/1‑270
                                                                                                                                                                                                                                                                                                          CCCGTCAACACATCGTTGGCGGGGATTTTAGCAATATTCGCTATTTTTATGTAATAATTTTATAAATGCGTTCAAAATAATAATCAAGTACTAATAGTGATATTTTAAGGTCTGATTTTTACGTGATAATTCAGGAGACACAGAATGCGCATAAAAATAACAGCATAAAACACCTTACCACCACCCAAGAATTTCATATTGTATTGTTTTTCAATGAAAAAATATTATTCGCGTAATATCTCACGATAAATAACATTAGGATTTTGTTAT  <  1:303034/270‑1
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
AAAAGTAGGATTTATCGATAAAGTAAGCAAGTGCAAAGGACTCGTGTTTAAATAACAAAATCCTAATGTTATTTATCGTGAGATATTACGCGAATAATATTTTTTCATTGAAAAACAATACAATATGAAATTCTTGGGTGGTGGTAAGGTGTTTTATGCTGTTATTTTTATGCGCATTCTGTGTCTCCTGAATTATCACGTAAAAATCAGACCTTAAAATATCACTATTAGTACTTGATTATTATTTTGAACGCATTTATAAAATTATTACATAAAAATAGCGAATATTGCTAAAATCCCCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/1572079‑1572380
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ccccgTCAACACATCGTTGGCGGGGATTTTAGCAATATTCGCTATTTTTATGTAATAATTTTATAAATGCGTTCAAAATAATAATCAAGTACTAATAGTGATATTTTAAGGTCTGATTTTTACGTGATAATTCAGGAGACACAGAATGCGCATAAAAATAACAGCATAAAACACCTTACCACCACCCAAGAATTTCATATTGTATTGTTTTTCAATGAAAAAATATTATTCGCGTAATATCTCACGATAAATAACATTAGGATTTTGTTAT  <  NC_000913/1572395‑1572130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.