breseq  version 0.29.0  revision 8f9c342918e4
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp [crl] [crl]
JC 702,444 (GCATAACGCGCACGCCCTGTTTCATCAGCTCATCGC)1→2 coding (308/1149 nt) nagA ← N‑acetylglucosamine‑6‑phosphate deacetylase
JC JC 889,488 IS1 (–) +9 bp coding (324‑332/1686 nt) ybjL ← putative transporter
RA 1,728,708 A→G N121S (AAT→AGT)  rnt → RNase T; exoribonuclease T; structured DNA 3' exonuclease; RNA processing; DNA repair
MC JC 1,978,503 Δ776 bp insB1insA insB1, insA
RA 2,173,363 Δ2 bp intergenic (‑1/+1) gatC ← / ← gatC pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,675,452 G→C P104A (CCG→GCG)  yphC ← putative Zn‑dependent NAD(P)‑binding oxidoreductase
RA 2,693,818 C→G V576L (GTG→CTG)  purL ← phosphoribosylformyl‑glycineamide synthetase
RA 2,701,175 G→C L185L (CTC→CTG pdxJ ← pyridoxine 5'‑phosphate synthase
RA 2,713,302 G→T R136L (CGT→CTT)  srmB → ATP‑dependent RNA helicase
RA 2,724,590 C→T G386D (GGT→GAT)  kgtP ← alpha‑ketoglutarate transporter
RA 3,548,179 G→T A631D (GCT→GAT)  malQ ← 4‑alpha‑glucanotransferase (amylomaltase)
RA 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
MC JC 4,130,167 Δ462 bp coding (333‑794/2433 nt) metL → Bifunctional aspartokinase/homoserine dehydrogenase 2
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3423729–3424234 3424586–3424238 5–858 32 [30] [31] 34 [rrfD]–[rrlD] [rrfD], [rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994980 (0.000)71 (1.180) 64/290 0.0 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein