Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	3,668,878	IS2 (+) +5 bp	coding (307‑311/1398 nt)	yhjA ←	putative cytochrome C peroxidase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	2996361 =	NA (NA)	45 (0.820)	37/294	0.9	100%	noncoding (1331/1331 nt)	IS2	repeat region
	?	NC_000913	3668878 =	0 (0.000)					coding (311/1398 nt)	yhjA	putative cytochrome C peroxidase
*	?	NC_000913	3186096 =	NA (NA)	51 (0.920)	45/298	0.4	100%	noncoding (1/1331 nt)	IS2	repeat region
	?	NC_000913	= 3668882	0 (0.000)					coding (307/1398 nt)	yhjA	putative cytochrome C peroxidase

TCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/3186236‑3186096 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTGGCTGATAAACCCGTTTCGCAAAGCGATTTGAATAAGATTGAATGGGTGATGCAGTATGAAACTATGCCACCAACGCGTTATACCGCGCTACACTGGGCGGGTAAGGTGAGTGATGAAGAGCGGGCGGAAATACTGGCCTGGATTGC  <  NC_000913/3668882‑3668734                                                                                                                                                                                                                                                                                                    TCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGAT                                                                                                                                              <  1:394284/150‑1          GTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCGTTT                                                                                                                                    <  1:640087/151‑1            ACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCGAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCGTTTCG                                                                                                                                  <  2:714815/151‑1            ACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCGTTTC                                                                                                                                   <  1:253700/150‑1              GCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCGTTTCGC                                                                                                                                 >  2:735513/1‑150                CGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCGTTTCGCAAA                                                                                                                              <  2:420496/151‑1                  TCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCGTTTCGCAAAGC                                                                                                                            >  2:599886/1‑151                      cgcTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAACAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGCCTACTGGCTGATAAACCCGTTTCGCAAAGTGATT                                                                                                                        >  1:240432/4‑151                      CGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCGTTTCGCAAAGCGATT                                                                                                                        >  1:713663/1‑151                              CGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCGTTTCGCAAAGCGATTTGAATAAG                                                                                                                <  2:732875/151‑1                               GGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCGTTTCGCAAAGCGATTTGAATAAGA                                                                                                               <  2:428459/151‑1                                 CCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAACCTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGTGCCAGTCTACTGGCTGATAAACCCGTTTCGCAAAGCGATTTGAATAAGATT                                                                                                             >  1:225016/1‑151                                 CCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCGTTTCGCAAAGCGATTTGGATAAGATT                                                                                                             <  1:10997/151‑1                                         CATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCGTTTCGCAAAGCGATTTGAATAAGATTGAATGGGT                                                                                                     <  1:69290/151‑1                                                 ATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCGTTTCGCAAAGCGATTTGAATAAGATTGAATGGGTGATGCAGT                                                                                             >  2:390525/1‑151                                                          CCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCGTTTCGCAAAGCGATTTGAATAAGATTGAATGGGTGATGCAGTATGAAACT                                                                                     <  1:580634/150‑1                                                           CAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCGTTTCGCAAAGCGATTTGAATAAGATTGAATGGGTGATGCAGTATGAAACTAT                                                                                   <  1:74849/151‑1                                                           CAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCGTTTCGCAAAGCGATTTGAATAAGATTGAATGGGTGATGCAGTATGAAA                                                                                       <  1:479816/147‑1                                                                ACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCGTTTCGCAAAGCGATTTGAATAAGATTGAATGGGTGATGCAGTATGAAACTATGCCAC                                                                              <  1:284072/151‑1                                                                 CTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCGTTTCGCAAAGCGATTTGAATAAGATTGAATGGGTGATGCAGTATGAAACTATGCCAC                                                                              <  1:248647/150‑1                                                                     TCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCGTTTCGCAAAGCGATTTGAATAAGATTGAATGGGTGATGCAGTATGAAACTATGCCACCAACG                                                                         >  1:385717/1‑151                                                                       TAACAACTAGTATTAAGTCTATCACTTATTTAAATGTTCTTGGTTGTCTGGAGATTCCGGGGGCCAGTCTACTGGATGATAAACCCGTTTCGCAAAGCGATTTGAATACGATTGAATGGGTGATGTAGTATGAAACAATGCCACCAACGCG                                                                       >  1:281215/1‑151                                                                        AAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCGTTTCGCAAAGCGATTTGAATAAGATTGAATGGGTGATGCAGTATGAAACTATGCCACCAACG                                                                         <  1:533443/148‑1                                                                               AGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCGTTTCGCAAAGCGATTTGAATAAGATTGAATGGGTGATGCAGTATGAAACTATGCCACCAACGCGTTATACCG                                                               <  1:560142/151‑1                                                                                  ATTAAGACTTTCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCGTTTCGCAAAGCGATTTGAATAAGATTGAATGGGTGATGCAGTATGAAACTATGCCACCAACGCGTTATACCGCGC                                                            >  2:444610/1‑151                                                                                      AGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCGTTTCGCAAAGCGATTTGAATAAGATTGAATGGGTGATGCAGTATGAAACTATGCCACCAACGCGTTATACCGCGCTACA                                                        <  2:697197/151‑1                                                                                      AGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCGTTTCGCAAAGCGATTTGAATAAGATTGAATGGGTGATGCAGTATGAAACTATGCCACCAACGCGTTATACCGCGCTAC                                                         >  1:737830/1‑150                                                                                      AGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCGTTTCGCAAAGCGATTTGAATAAGATTGAATGGGTGATGCAGTATGAAACTATGCCACCAACGCGTTATACCGCGCTAC                                                         >  1:79940/1‑150                                                                                           ATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCGTTTCGCAAAGCGATTTGAATAAGATTGAATGGGTGATGCAGTATGAAACTATGCCACCAACGCGTTATACCGCGCTACACTGG                                                    <  2:217521/150‑1                                                                                              ACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCGTTTCGCAAAGCGATTTGAATAAGATTGAATGGGTGATGCAGTATGAAACTATGCCACCAACGCGTTATACCGCGCTACACTGGGCGG                                                <  2:53383/151‑1                                                                                               CTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCGTTTCGCAAAGCGATTTGAATAAGATTGAATGGGTGATGCAGTATGAAACTATGCCACCAACGCGTTATACCGCGCTACACTGGGCGGG                                               >  2:806124/1‑151                                                                                                 TATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCGTTTCGCAAAGCGATTTGAATAAGATTGAATGGGTGATGCAGTATGAAACTATGCCACCAACGCGTTATACCGCGCTACACTGGGCGGGT                                              >  1:800667/1‑150                                                                                                   TTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCGTTTCGCAAAGCGATTTGAATAAGATTGAATGGGTGATGCAGTATGAAACTATGCCACCAACGCGTTATACCGCGCTACACTGGGCGGGTA                                             >  1:784692/1‑149                                                                                                   TTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCGTTTCGCAAAGCGATTTGAATAAGATTGAATGGGTGATGCAGTATGAAACTATGCCACCAACGCGTTATACCGCGCTACACTGGGCGGGT                                              >  1:666192/1‑148                                                                                                     TAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCGTTTCGCAAAGCGATTTGAATAAGATTGAATGGGTGATGCAGTATGAAACTATGCCACCAACGCGTTATACCGCGCTACACTGGGCGGGTAAGGT                                         >  2:608564/1‑151                                                                                                      AAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCGTTTCGCAAAGCGATTTGAATAAGATTGAATGGGTGATGCAGTATGAAACTATGCCACCAACGCGTTATACCGCGCTACACTGGGCGGGTAAGGTG                                        >  2:74685/1‑151                                                                                                        GTGATATTGGGTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACGCGTTTCGCAAAGCGATTTGAATAAGATTGAATGGGTGATGCAGTATGAAACTATGCCACCAACGCGTTATACCGCGCTACACTGGGCGGGTAAGGTG                                        >  1:181163/1‑149                                                                                                          GATCTTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCGTTTCGCAAAGCGATTTGAATAAGATTGAATGGGTGATGCAGTATGAAACTATGCCACCAACGCGTTATACCGCGCTACACTGGGCGGGTAAGGTGAGTG                                    >  1:155569/1‑151                                                                                                           ATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCGTTTCGCAAAGCGATTTGAATAAGATTGAATGGGTGATGCAGTATGAAACTATGCCACCAACGCGTTATACCGCGCTACACTGGGCGGGTAAGGTGAGTG                                    >  1:758086/1‑150                                                                                                            TATTAGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCGTTTCGCAAAGCGATTTGAATAAGATTGAATGGGTGATGCAGTATGAAACTATGCCACCAACGCGTTATACCGCGCTACACTGGGCGGGTAAGGTGAGTGAT                                  >  1:779856/1‑151                                                                                                               TGGTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCGTTTCGCAAAGCGATTTGAATAAGATTGAATGGGTGATGCAGTATGAAACTATGCCACCAACGCGTTATACCGCGCTACACTGGGCGGGTAAGGTGAGTGATGA                                <  2:821545/150‑1                                                                                                                 GTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCGTTTCGCAAAGCGATTTGAATAAGATTGAATGGGTGATGCAGTATGAAACTATGCCACCAACGCGTTATACCGCGCTACACTGGGCGGGTAAGGTGAGTGATGAAGA                             <  2:779952/151‑1                                                                                                                 GTTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCGTTTCGCAAAGCGATTTGAATAAGATTGAATGGGTGATGCAGTATGAAACTATGCCACCAACGCGTTATACCGCGCTACACTGGGCGGGTAAGGTGAGTGATGA                                >  1:694009/1‑148                                                                                                                  TTGTCTGGAGATTCAGGGGGCCAGTCTACTGGCTGATAAACCCGTTTCGCAAAGCGATTTGAATAAGATTGAATGGGTGATGCAGTATGAAACTATGCCACCAACGCGTTATACCGCGCTACACTGGGCGGGTAAGGTGAGTGATGAAG                              >  1:869496/1‑149                                                                                                                          AGATTCAGGGGGCCAGTCTACTGGCCGATAAACCCGTTTCGCAAAGCGATTTGAATAAGATTGAATGGGTGATGCAGTATGAAACTATGCCACCAACGCGTTATACCGCGCTACACTGGGCGGGTAAGGTGAGTGATGAAGAGCGGGCGG                     <  1:67333/150‑1                                                                                                                               CAGGGGGCCAGTCTACTGGCTGATAAACCCGTTTCGCAAAGCGATTTGAATAAGATTGAATGGGTGATGCAGTATGAAACTATGCCACCAACGCGTTATACCGCGCTACACTGGGCGGGTAAGGTGAGTGATGAAGAGCGGGCGGAAATAC               >  2:852343/1‑151                                                                                                                                  tGGGCCAGTCTACTGGCTGATAAACCCGTTTCGCAAAGCGCTTTGAATAAGATTGAATGGGTGATGCAGTATGAAACTATGCCACCAACGCGTTATACCGCGCTACACTGGGCGGGTAAGGTGAGTGATGAATAGCGGGCGGAAATACTGG            >  1:521560/2‑151                                                                                                                                     GCCAGTCTACTGGCTGATAAACCCGTTTCGCAAAGCGATTTGAATAAGATTGAATGGGTGATGCAGTATGAAACTATGCCACCAACGCGTTATACCGCGCTACACTGGGCGGGTAAGGTGAGTGATGAAGAGCGGGCGGAAATACTGGCCT         <  2:683374/151‑1                                                                                                                                             ACTGGCTGATAAACCCGTTTCGCAAAGCGATTTGAATAAGATTGAATGGGTGATGCAGTATGAAACTATGCCACCAACGCGTTATACCGCGCTACACTGGGCGGGTAAGGTGAGTGATGAAGAGCGGGCGGAAATACTGGCCTGGATTGC  <  1:829939/150‑1                                                                                                                                             ACTGGCTGATAAACCCGTTTCGCAAAGCGATTTGAATAAGATTGAATGGGTGATGCAGTATGAAACTATGCCACCAACGCGTTATACCGCGCTACACTGGGCGGGTAAGGTGAGTGATGAAGAGCGGGCGGAAATACTGGCCTGGATT    <  2:483250/148‑1                                                                                                                                             ACTGGCTGATAAACCCGTTTCGCAAAGCGATTTGAATAAGATTGAATGGGTGATGCAGTATGAAACTATGCCACCAACGCGTTATACCGCGCTACACTGGGCGGGTAAGGTGAGTGATGAAGAGCGGGCGGAAATACTGGCCTGGATT    <  2:701187/148‑1                                                                                                                                                                                                                                                                                                    TCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/3186236‑3186096 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTGGCTGATAAACCCGTTTCGCAAAGCGATTTGAATAAGATTGAATGGGTGATGCAGTATGAAACTATGCCACCAACGCGTTATACCGCGCTACACTGGGCGGGTAAGGTGAGTGATGAAGAGCGGGCGGAAATACTGGCCTGGATTGC  <  NC_000913/3668882‑3668734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.