Predicted mutation
evidence seq id position mutation annotation gene description
JC NC_000913 3,277,273 (TTCAACA)2→3 coding (272/336 nt) prlF → antitoxin of the SohA(PrlF)‑YhaV toxin‑antitoxin system

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 32772570 (0.000)38 (1.160)
+CATTCAA
35/252 0.0 100% coding (256/336 nt) prlF antitoxin of the SohA(PrlF)‑YhaV toxin‑antitoxin system
?NC_000913 3277258 = 0 (0.000)coding (257/336 nt) prlF antitoxin of the SohA(PrlF)‑YhaV toxin‑antitoxin system

AGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTATGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACG  >  NC_000913/3277108‑3277403
                                                                                                                                                     |                                                                                                                                                  
aGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTATGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCc                                                                                                                                                   >  2:225652‑M1/1‑150 (MQ=255)
      gccaggACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTATGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaa                                                                                                                                             <  2:200068‑M1/151‑8 (MQ=255)
         aggACAGCATTCATTATGAAATTCTGCCTGGTGGGCAAGTATTTATGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacat                                                                                                                                          >  2:20929‑M1/1‑141 (MQ=255)
         aggACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTATGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacat                                                                                                                                          >  1:428245‑M1/1‑141 (MQ=255)
           gACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTATGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattc                                                                                                                                        >  1:273899‑M1/1‑139 (MQ=255)
             cAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTATGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattca                                                                                                                                       >  1:417292‑M1/1‑137 (MQ=255)
              aGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTATGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaac                                                                                                                                     >  1:481707‑M1/1‑136 (MQ=255)
                cattcattACGAAATTCTGCCTGGTGGGCAAGTATTTATGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaacat                                                                                                                                   >  2:136039‑M1/1‑134 (MQ=255)
                      ttACGAAATTCTGCCTGGTGGGCAAGTATTTATGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaacattcaac                                                                                                                              >  1:534111‑M1/1‑128 (MQ=255)
                         cGAAATTCTGCCTGGTGGGCAAGTATTTATGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaacattcaacaagg                                                                                                                          >  1:317643‑M1/1‑125 (MQ=255)
                               tCTGCCTGGTGGGCAAGTATTTATGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaacattcaacaaggaaaga                                                                                                                     >  2:283052‑M1/1‑119 (MQ=255)
                                  gCCTGGTGGGCAAGTATTTATGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaacattcaacaaggaaagaaact                                                                                                                 >  2:213366‑M1/1‑116 (MQ=255)
                                  gCCTGGTGGGCAAGTATTTATGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaacattcaacaaggaaagaaa                                                                                                                   <  1:268695‑M1/149‑34 (MQ=255)
                                    cTGGTGGGCAAGTATTTATGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaacattcaacaaggaaagaaacttg                                                                                                               >  1:345420‑M1/1‑114 (MQ=255)
                                         gggCAAGTATTTATGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgct                                                                                                          >  2:363905‑M1/1‑109 (MQ=255)
                                         gggCAAGTATTTATGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgc                                                                                                           <  2:394331‑M1/150‑42 (MQ=255)
                                                 aTTTATGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatgga                                                                                                  <  1:497557‑M1/151‑51 (MQ=255)
                                                   ttATGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggacg                                                                                                >  2:82866‑M1/1‑99 (MQ=255)
                                                   ttATGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggac                                                                                                 <  1:27643‑M1/150‑52 (MQ=255)
                                                    tATGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggacgt                                                                                               >  1:289205‑M1/1‑98 (MQ=255)
                                                          ccGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggacgtcaacat                                                                                         <  2:192135‑M1/151‑60 (MQ=255)
                                                           cGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggacgtcaacatt                                                                                        >  2:283471‑M1/1‑91 (MQ=255)
                                                            gACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggacgtcaacattg                                                                                       >  2:224155‑M1/1‑90 (MQ=255)
                                                                                gaTCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggacgtcaacattgatgatgagattggcgacgac                                                                   <  2:404688‑M1/151‑82 (MQ=255)
                                                                                 aTCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggacgtcaacattgatgatgagattggcgacgacg                                                                  >  2:136219‑M1/1‑69 (MQ=255)
                                                                                      aCTATGAATGCATTTTTGCGCTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggacgtcaacattgatgatgagattggcgacgacgaata                                                              <  2:111445‑M1/150‑87 (MQ=255)
                                                                                           gAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggacgtcaacattgatgatgagattggcgacgacgaataatggat                                                        <  1:468960‑M1/151‑93 (MQ=255)
                                                                                                   ttttGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggacgtcaacattgatgatgagattggcgacgacgaataatggattttccac                                                 <  2:282038‑M1/150‑100 (MQ=255)
                                                                                                           tttCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggacgtcaacattgatgatgagattggcgacgacgaataatggattttccacaaagggtt                                         >  2:31367‑M1/1‑43 (MQ=255)
                                                                                                           tttCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggacgtcaacattgatgatgagattggcgacgacgaataatggattttccacaaagggtt                                         <  2:424025‑M1/150‑108 (MQ=255)
                                                                                                                ggATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggacgtcaacattgatgatgagattggcgacgacgaataatggattttccacaaagggttaat                                      <  2:471546‑M1/148‑111 (MQ=255)
                                                                                                                      aGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggacgtcaacattgatgatgagattggcgacgacgaataatggattttccacaaagggttaatggttgggcg                             <  2:13461‑M1/151‑120 (MQ=255)
                                                                                                                       gATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggacgtcaacattgatgatgagattggcgacgacgaataatggattttccacaaagggttaatggttgggcgc                            <  1:282973‑M1/151‑121 (MQ=255)
                                                                                                                            ccAGAACAACCCGCAAAAAACTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggacgtcaacattgatgatgagattggcgacgacgaataatggattttccacaaagggttaatggttgggcgctatat                       >  1:472394‑M1/1‑26 (MQ=255)
                                                                                                                             cataacaacccgCAAAAAACTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggacgtcaacattgatgatgagattggcgacgacgaataatggattttccacaaagggttaatggttgggcgctatatg                      <  1:195356‑M1/148‑127 (MQ=255)
                                                                                                                                 acaacCCGCAAAAAACTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggacgtcaacattgatgatgagattggcgacgacgaataatggattttccacaaagggttaatggttgggcgctatatgctc                   >  1:984‑M1/1‑21 (MQ=255)
                                                                                                                                   aacCCGCAAAAAACTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggacgtcaacattgatgatgagattggcgacgacgaataatggattttccacaaagggttaatggttgggcgctatatgctcatc                >  2:406735‑M1/1‑19 (MQ=255)
                                                                                                                                    acCCGCAAAAAACTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggacgtcaacattgatgatgagattggcgacgacgaataatggattttccacaaagggttaatggttgggcgctatatgctcatcc               >  2:60558‑M1/1‑18 (MQ=255)
                                                                                                                                       cGCAAAAAACTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggacgtcaacattgatgatgagattggcgacgacgaataatggattttccacaaagggttaatggttgggcgctatatgctcatccctg            <  1:503407‑M1/151‑137 (MQ=255)
                                                                                                                                        gCAAAAAACTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggacgtcaacattgatgatgagattggcgacgacgaataatggattttccacaaagggttaatggttgggcgctatatgctcatccctgt           <  2:331475‑M1/151‑138 (MQ=255)
                                                                                                                                             aaaCTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggacgtcaacattgatgatgagattggcgacgacgaataatggattttccacaaagggttaatggttgggcgctatatgctcatccctgttttca      <  2:318835‑M1/151‑143 (MQ=255)
                                                                                                                                                  cGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggacgtcaacattgatgatgagattggcgacgacgaataatggattttccacaaagggttaatggttgggcgctatatgctcatccctgttttcaggaa  >  2:523501‑M1/1‑4 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
AGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTATGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACG  >  NC_000913/3277108‑3277403

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.