Predicted mutation
evidence seq id position mutation annotation gene description
JC NC_000913 3,277,273 (TTCAACA)2→3 coding (272/336 nt) prlF → antitoxin of the SohA(PrlF)‑YhaV toxin‑antitoxin system

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 32772570 (0.000)38 (1.160)
+CATTCAA
35/252 0.0 100% coding (256/336 nt) prlF antitoxin of the SohA(PrlF)‑YhaV toxin‑antitoxin system
?NC_000913 3277258 = 0 (0.000)coding (257/336 nt) prlF antitoxin of the SohA(PrlF)‑YhaV toxin‑antitoxin system

CCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTATGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAA  >  NC_000913/3277110‑3277396
                                                                                                                                                    |                                                                                                                                          
aggacagcattcattatgaaattctgcctggtgggcaagtatttatgtgccgactgggagatgaacaggaggatcatactatgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaacat                                                                                                                                          >  2:20929‑M2/149‑151 (MQ=255)
aggacagcattcattacgaaattctgcctggtgggcaagtatttatgtgccgactgggagatgaacaggaggatcatactatgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaacat                                                                                                                                          >  1:428245‑M2/149‑151 (MQ=255)
  gacagcattcattacgaaattctgcctggtgggcaagtatttatgtgccgactgggagatgaacaggaggatcatactatgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaacattc                                                                                                                                        >  1:273899‑M2/147‑151 (MQ=255)
    cagcattcattacgaaattctgcctggtgggcaagtatttatgtgccgactgggagatgaacaggaggatcatactatgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaacattca                                                                                                                                       >  1:417292‑M2/145‑150 (MQ=255)
     agcattcattacgaaattctgcctggtgggcaagtatttatgtgccgactgggagatgaacaggaggatcatactatgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaacattcaac                                                                                                                                     >  1:481707‑M2/144‑151 (MQ=255)
       cattcattacgaaattctgcctggtgggcaagtatttatgtgccgactgggagatgaacaggaggatcatactatgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaacattcaacat                                                                                                                                   >  2:136039‑M2/142‑151 (MQ=255)
             ttacgaaattctgcctggtgggcaagtatttatgtgccgactgggagatgaacaggaggatcatactatgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaacattcaacattcaac                                                                                                                              >  1:534111‑M2/136‑150 (MQ=255)
                cgaaattctgcctggtgggcaagtatttatgtgccgactgggagatgaacaggaggatcatactatgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaaCATTCAACATTCAACAAgg                                                                                                                          >  1:317643‑M2/133‑151 (MQ=255)
                      tctgcctggtgggcaagtatttatgtgccgactgggagatgaacaggaggatcatactatgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaaCATTCAACATTCAACAAGgaaaga                                                                                                                     >  2:283052‑M2/127‑150 (MQ=255)
                         gcctggtgggcaagtatttatgtgccgactgggagatgaacaggaggatcatactatgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaaCATTCAACATTCAACAAGgaaagaaa                                                                                                                   <  1:268695‑M2/26‑1 (MQ=255)
                         gcctggtgggcaagtatttatgtgccgactgggagatgaacaggaggatcatactatgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaaCATTCAACATTCAACAAGGAAAGAAACt                                                                                                                 >  2:213366‑M2/124‑151 (MQ=255)
                           ctggtgggcaagtatttatgtgccgactgggagatgaacaggaggatcatactatgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaaCATTCAACATTCAACAAGGAAAGAAACTTg                                                                                                               >  1:345420‑M2/122‑151 (MQ=255)
                                gggcaagtatttatgtgccgactgggagatgaacaggaggatcatactatgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaaCATTCAACATTCAACAAGGAAAGAAACTTGTCGc                                                                                                           <  2:394331‑M2/34‑1 (MQ=255)
                                gggcaagtatttatgtgccgactgggagatgaacaggaggatcatactatgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaaCATTCAACATTCAACAAGGAAAGAAACTTGTCGCt                                                                                                          >  2:363905‑M2/117‑151 (MQ=255)
                                        atttatgtgccgactgggagatgaacaggaggatcatactatgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaaCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGa                                                                                                  <  1:497557‑M2/43‑1 (MQ=255)
                                          ttatgtgccgactgggagatgaacaggaggatcatactatgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaaCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGAc                                                                                                 <  1:27643‑M2/44‑1 (MQ=255)
                                          ttatgtgccgactgggagatgaacaggaggatcatactatgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaaCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACg                                                                                                >  2:82866‑M2/107‑151 (MQ=255)
                                           tatgtgccgactgggagatgaacaggaggatcatactatgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaaCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGt                                                                                               >  1:289205‑M2/106‑151 (MQ=255)
                                                 ccgactgggagatgaacaggaggatcatactatgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaaCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACAt                                                                                         <  2:192135‑M2/52‑1 (MQ=255)
                                                  cgactgggagatgaacaggaggatcatactatgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaaCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACAtt                                                                                        >  2:283471‑M2/99‑151 (MQ=255)
                                                   gactgggagatgaacaggaggatcatactatgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaaCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATtg                                                                                       >  2:224155‑M2/98‑151 (MQ=255)
                                                                       gatcatactatgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaaCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGcgacgac                                                                   <  2:404688‑M2/74‑1 (MQ=255)
                                                                        atcatactatgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaaCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGcgacgacg                                                                  >  2:136219‑M2/77‑151 (MQ=255)
                                                                             actatgaatgcatttttgcgctttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaaCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGaata                                                              <  2:111445‑M2/79‑1 (MQ=255)
                                                                                  gaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaaCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGAt                                                        <  1:468960‑M2/85‑1 (MQ=255)
                                                                                          ttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaaCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCcac                                                 <  2:282038‑M2/92‑1 (MQ=255)
                                                                                                  tttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaaCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGtt                                         <  2:424025‑M2/100‑1 (MQ=255)
                                                                                                  tttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaaCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGtt                                         >  2:31367‑M2/51‑150 (MQ=255)
                                                                                                       ggatgcagatatccagaacaacccgcaaaaaactcgtccattcaaCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAAt                                      <  2:471546‑M2/103‑1 (MQ=255)
                                                                                                             agatatccagaacaacccgcaaaaaactcgtccattcaaCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTGGgcg                             <  2:13461‑M2/112‑1 (MQ=255)
                                                                                                              gatatccagaacaacccgcaaaaaactcgtccattcaaCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTGGgcgc                            <  1:282973‑M2/113‑1 (MQ=255)
                                                                                                                   ccagaacaacccgcaaaaaactcgtccattcaaCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTatat                       >  1:472394‑M2/34‑151 (MQ=255)
                                                                                                                    cataacaacccgcaaaaaactcgtccattcaaCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATg                      <  1:195356‑M2/119‑1 (MQ=255)
                                                                                                                        acaacccgcaaaaaactcgtccattcaaCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTc                   >  1:984‑M2/29‑150 (MQ=255)
                                                                                                                          aacccgcaaaaaactcgtccattcaaCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATc                >  2:406735‑M2/27‑151 (MQ=255)
                                                                                                                           acccgcaaaaaactcgtccattcaaCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATcc               >  2:60558‑M2/26‑151 (MQ=255)
                                                                                                                              cgcaaaaaactcgtccattcaaCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTg            <  1:503407‑M2/129‑1 (MQ=255)
                                                                                                                               gcaaaaaactcgtccattcaaCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGt           <  2:331475‑M2/130‑1 (MQ=255)
                                                                                                                                    aaactcgtccattcaaCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCa      <  2:318835‑M2/135‑1 (MQ=255)
                                                                                                                                         cgtccattcaaCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGaa  >  2:523501‑M2/12‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                          
CCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTATGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAA  >  NC_000913/3277110‑3277396

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.