Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	3,328,463	IS4 (–) +11 bp	coding (243‑253/477 nt)	greA ←	transcript cleavage factor

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	3328463 =	0 (0.000)	50 (1.100)	46/298	0.1	100%	coding (253/477 nt)	greA	transcript cleavage factor
	?	NC_000913	= 4503515	NA (NA)					noncoding (1/1426 nt)	IS4	repeat region
*	?	NC_000913	= 3328473	0 (0.000)	47 (1.050)	42/294	0.2	100%	coding (243/477 nt)	greA	transcript cleavage factor
	?	NC_000913	4502090 =	NA (NA)					noncoding (1426/1426 nt)	IS4	repeat region

AGCGCGTGAGCATGGCGACCTGAAAGAAAACGCCGAATACCACGCAGCTCGTGAACAGCAGGGTTTCTGCGAAGGCCGTATTAAAGACATCGAAGCCAAGCTGTCGAACGCGCAGGTGATTGATGTCACCAAAATGCCCAACAATGGGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/3328611‑3328463 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAAACGCTCACTTGTTTCCGGATTTTTTTATGCACATTGGACAGGCTCTTGATCTGGTATCCCGTTACGATTCTCTGCGTAACCCACTGACTTC  <  NC_000913/4503515‑4503367                                                                                                                                                                                                                                                                                                            AGCGCGTGAGCATGGCGACCTGAAAGAAAACGCCGAATACCACGCAGCTCGTGAACAGCAGGGTTTCTGCGAAGGCCGTATTAAAGACATCGAAGCCAAGCTGTCGAACGCGCAGGTGATTGATGTCACCAAAATGCCCAACAATGGGCT                                                                                                                                                      >  2:57431/1‑150           CATGGCGACCTGAAAGAAAACGCCGAATACCACGCAGCTCGTGAACAGCAGGGTTTCTGCGAAGGCCGTATTAAAGACATCGAAGCCAAGCTGTCGAACGCGCAGGTGATTGATGTCACCAAAATGCCCAACAATGGGCTAATGCCGATCA                                                                                                                                           <  1:20869/151‑1                      GAAAGAAAACGCCGAATACCACGCAGCTCGTGAACAGCAGGGTTTCTGCGAAGGCCGTATTAAAGACATCGAAGCCAAGCTGTCGAACGCGCAGGTGATTGATGTCACCAAAATGCCCAACAATGGGCTAATGCCGATCAGTTAAGGATC                                                                                                                                 <  2:130148/150‑1                       AAAGAAAACGCCGAATACCACGCAGCTCGTGAACAGCAGGGTTTCTGCGAAGGCCGTATTAAAGACATCGAAGCCAAGCTGTCGAACGCGCAGGTGATTGATGTCACCAAAATGCCCAACAATGGGCTAATGCCGATCAGTTAAGGATC                                                                                                                                 >  1:354932/1‑149                         AGAAAACGCCGAATACCACGCAGCTCGTGAACAGCAGGGTTTCTGCGAAGGCCGTATTAAAGACATCGAAGCCAAGCTGTCGAACGCGCAGGTGATTGATGTCACCAAAATGCCCAACAATGGGCTAATGCCGATCAGTTAAGGATCAGTT                                                                                                                             <  2:330919/151‑1                             AACGCCGAATACCACGCAGCTCGTGAACAGCAGGGTTTCTGCGAAGGCCGTATTAAAGACATCGAAGCCAAGCTGTCGAACGCGCAGGTGATTGATGTCACCAAAATGCCCAACAATGGGCTAATGCCGATCAGTTAAGGATCAGTTGACC                                                                                                                         >  2:440191/1‑151                              ACGCCGAATACCACGCAGCTCGTGAACAGCAGGGTTTCTGCGAAGGCCGTATTAAAGACATCGAAGCCAAGCTGTCGAACGCGCAGGTGATTGATGTCACCAAAATGCCCAACAATGGGCTAATGCCGATCAGTTAAGGATCAGTTGACCG                                                                                                                        >  1:33286/1‑151                               CGCCGAATACCACGCAGCTCGTGAACAGCAGGGTTTCTGCGAAGGCCGTATTAAAGACATCGAAGCCAAGCTGTCGAACGCGCAGGTGATTGATGTCACCAAAATGCCCAACAATGGGCTAATGCCGATCAGTTAAGGATCAGTTGACCGA                                                                                                                       <  1:692300/151‑1                                    AATACCACGCAGCTCGTGAACAGCAGGGTTTCTGCGAAGGCCGTATTAAAGACATCGAAGCCAAGCTGTCGAACGCGCAGGTGATTGATGTCACCAAAATGCCCAACAATGGGCTAATGCCGATCAGTTAAGGATCAGTTGACCGATCC                                                                                                                    >  2:240469/1‑149                                              AGCTCGTGAACAGCAGGGTTTCTGCGAAGGCCGTATTAAAGACATCGAAGCCAAGCTGTCGAACGCGCAGGTGATTGATGTCACCAAAATGCCCAACAATGGGCTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGT                                                                                                         >  2:148890/1‑150                                              AGCTCGTGAACAGCAGGGTTTCTGCGAAGGCCGTATTAAAGACATCGAAGCCAAGCTGTCGAACGCGCAGGTGATTGATGTCACCAAAATGCCCAACAATGGGCTAATGCCAATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTA                                                                                                        <  2:483349/151‑1                                                  CGTGAACAGCAGGGTTTCTGCGAAGGCCGTATTAAAGACATCGAAGCCAAGCTGTCGAACGCGCAGGTGATTGATGTCACCAAAATGCCCAACAATGGGCTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAA                                                                                                    <  2:386052/151‑1                                                  CGTGAACAGCAGGGTTTCTGCGAAGGCCGTATTAAAGACATCGAAGCCAAGCTGTCGAACGCGCAGGTGATTGATGTCACCAAAATGCCCAACAATGGGCTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGA                                                                                                     <  1:446140/150‑1                                                  CGTGAACAGCAGGGTTTCTGCGAAGGCCGTATTAAAGACATCGAAGCCAAGCTGTCGAACGCGCAGGTGATTGATGTCACCAAAATGCCCAACAATGGGCTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGA                                                                                                     >  2:54857/1‑150                                                   GTGAACAGCAGGGTTTCTGCGAAGGCCGTATTAAAGACATCGAAGCCAAGCTGTCGAACGCGCAGGTGATTGATGTCACCAAAATGCCCAACAATGGGCTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAAT                                                                                                   >  1:637781/1‑151                                                      AACAGCAGGGTTTCTGCGAAGGCCGTATTAAAGACATCGAAGCCAAGCTGTCGAACGCGCAGGTGATTGATGTCACCAAAATGCCCAACAATGGGCTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCG                                                                                                <  2:429068/151‑1                                                      AACAGCAGGGTTTCTGCGAAGGCCGTATTAAAGACATCGAAGCCAAGCTGTCGAACGCGCAGGTGATTGATGTCACCAAAATGCCCAACAATGGGCTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCG                                                                                                >  2:306800/1‑151                                                         AGCAGGGTTTCTGCGAAGGCCGTATTAAAGACATCGAAGCCAAGCTGTCGAACGCGCAGGTGATTGATGTCACCAAAATGCCCAACAATGGGCTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAA                                                                                             <  1:64220/151‑1                                                                 TTCTGCGAAGGCCGTATTAAAGACATCGAAGCCAAGCTGTCGAACGCGCAGGTGATTGATGTCACCAAAATGCCCAACAATGGGCTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAAACGCTCA                                                                                      <  1:303369/150‑1                                                                     GCGAAGGCCGTATTAAAGACATCGAAGCCAAGCTGTCGAACGCGCAGGTGATTGATGTCACCAAAATGCCCAACAATGGGCTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAAACGCTCACTT                                                                                   <  2:384470/149‑1                                                                       GAAGGCCGTATTAAAGACATCGAAGCCAAGCTGTCGAACGCGCAGGTGATTGATGTCACCAAAATGCCCAACAATGGGCTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAAACGCTCACTTGTTT                                                                               >  2:606813/1‑151                                                                             CGTATTAAAGACATCGAAGCCAAGCTGTCGAACGCGCAGGTGATTGATGTCACCAAAATGCCCAACAATGGGCTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAAACGCTCACTTGTTTCCGGAT                                                                         >  2:169654/1‑151                                                                              GTATTAAAGACATCGAAGCCAAGCTGTCGAACGCGCAGGTGATTGATGTCACCAAAATGCCCAACAATGGGCTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAAACGCTCACTTGTTTCCGGATT                                                                        <  1:35392/151‑1                                                                                   AAAGACATCGAAGCCAAGCTGTCGAACGCGCAGGTGATTGATGTCACCAAAATGCCCAACAATGGGCTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAAACGCTCACTTGTTTCCGGATTTTTT                                                                    <  1:61314/150‑1                                                                                     AGACATCGAAGCCAAGCTGTCGAACGCGCAGGTGATTGATGTCACCAAAATGCCCAACAATGGGCTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAAACGCTCACTTGTTTCCGGATTTTTTTAT                                                                 <  1:356447/151‑1                                                                                      GACATCGAAGCCAAGCTGTCGAACGCGCAGGTGATTGATGTCACCAAAATGCCCAACAATGGGCTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAAACGCTCACTTGTTTCCGGATTTTTTTATG                                                                <  1:602075/151‑1                                                                                         ATCGAAGCCAAGCTGTCGAACGCGCAGGTGATTGATGTCACCAAAATGCCCAACAATGGGCTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAAACGCTCACTTGTTTCCGGATTTTTTTATGCAC                                                             >  2:384986/1‑151                                                                                             AAGCCAAGCTGTCGAACGCGCAGGTGATTGATGTCACCAAAATGCCCAACAATGGGTTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAAACGCTCACTTGTTTCCGGATTTTTTTATGCACATTG                                                         <  2:176385/151‑1                                                                                             AAGCCAAGCTGTCGAACGCGCAGGTGATTGATGTCACCAAAATGCCCAACAATGGGCTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAAACGCTCACTTGTTTCCGGATTTTTTTATGCACATTG                                                         <  2:318441/151‑1                                                                                             AAGCCAAGCTGTCGAACGCGCAGGTGATTGATGTCACCAAAATGCCCAACAATGGGCTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAAACGCTCACTTGTTTCCGGATTTTTTTATGCACATTG                                                         >  2:222540/1‑151                                                                                                CCAAGCTGTCGAACGCGCAGGTGATTGATGTCACCAAAATGCCCAACAATGGGCTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAAACGCTCACTTGTTTCCGGATTTTTTTATGCACATTGGA                                                       >  1:478412/1‑150                                                                                                         CGAATGCGCAGGTGATTGATGTCACCAAAATGCCCAACAATGGGCTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAAACGCTCACTTGTTTCCGGATTTTTTTATGCACATTGGACAGGCTCTTG                                             <  2:707517/151‑1                                                                                                          GAACGCGCAGGTGATTGATGTCACCAAAATGCCCAACAATGGGCTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAAACGCTCACTTGTTTCCGGATTTTTTTATGCTCATTGGACAGGCTCTTGA                                            <  2:470506/151‑1                                                                                                           AACGCGCAGGTGATTGATGTCACCAAAATGCCCAACAATGGGCTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAAACGCTCACTTGTTTCCGGATTTTTTTATGCACATTGGACAGGCTCTTGAT                                           >  1:417258/1‑151                                                                                                           AACGCGCAGGTGATTGATGTCACCAAAATGCACAACAATGGGCTAATGCCGATCAGTTAAGGATCAGTTGACTGATCCAGTGGCTGTGTAAGAATCCGGAAACGCTCACTTGTTTCCGGATTTTTTTATGCACATTGGACAGGCTCTTGA                                            <  1:291953/150‑1                                                                                                              GCGCAGGTGATTGATGTCACCAAAATGCCCAACAATGGGCTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAAACGCTCACTTGTTTCCGGATTTTTTTATGCACATTGGACAGGCTCTTGATCTG                                        <  2:608445/151‑1                                                                                                                       ATTGATGTCACCAAAATGCCCAACAATGGGCTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAAACGCTCACTTGTTTCCGGATTTTTTTATGCACATTGGACAGGCTCTTGATCTGGTATCCCGT                               >  1:191237/1‑151                                                                                                                        TTGATGTCACCAAAATGCCCAACAATGGGCTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAAACGCTCACTTGTTTCCGGATTTTTTTATGCACATTGGACAGGCTCTTGATCTGGTATCCCGTT                              >  1:680952/1‑151                                                                                                                        TTGATGTCACCAAAATGCCCAACAATGGGCTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAAACGCTCACTTGTTTCCGGATTTTTTTATGCACATTGGACAGGCTCTTGATCTGGTATCCCGTT                              >  1:37167/1‑151                                                                                                                          GATGTCACCAAAATGCCCAACAATGGGCTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAAACGCTCACTTGTTTCCGGATTTTTTTATGCACATTGGACAGGCTCTTGATCTGGTATCCCGTTA                             <  1:69922/150‑1                                                                                                                             GTCACCAAAATGCCCAACAATGGGCTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAAACGCTCACTTGTTTCCGGATTTTTTTATGCACATTGGACAGGCTCTTGATCTGGTATCCCGTTACGAT                         <  1:305868/151‑1                                                                                                                               CACCAAAATGCCCAACAATGGGCTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAAACGCTCACTTGTTTCCGGATTTTTTTATGCACATTGGACAGGCTCTTGATCTGGTATCCCGTTACGATT                        <  1:531328/150‑1                                                                                                                                   AAAATGCCCAACAATGGGCTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAAACGCTCACTTGTTTCCGGATTTTTTTATGCACATTGGACAGGCTCTTGATCTGGTATCCCGTTACGATTCTCTG                   >  1:96126/1‑151                                                                                                                                   AAAATGCCCAACAATGGGCTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAAACGCTCACTTGTTTCCGGATTTTTTTATGCACATTGGACAGGCTCTTGATCTGGTATCCCGTTACGACTCTCT                    >  2:676836/1‑150                                                                                                                                      ATGCCCAACAATGGGCTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAAACGCTCACTTGTTTCCGGATTTTTTTATGCACATTGGACAGGCTCTTGATCTGGTATCCCGTTACGATTCTCTGCGT                <  1:337992/151‑1                                                                                                                                        GCCCAACAATGGGCTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAAACGCTCACTTGTTTCCGGATTTTTTTATGCACATTGGACAGGCTCTTGATCTGGTATCCCGTTACGATTCTCTGCGTA               <  2:247095/150‑1                                                                                                                                          CCAACAATGGGCTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAAACGCTCACTTGTTTCCGGATTTTTTTATGCACATTGGACAGGCTCTTGATCTGGTATCCCGTTACGATTCTCTGCGTAACC            <  1:54863/151‑1                                                                                                                                          CCAACAATGGGCTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAAACGCTCACTTGTTTCCGGATTTTTTTATGCACATTGGACAGGCTCTTGATCTGGTATCCCGTTACGATTCTCTGCGTAACC            >  2:37314/1‑151                                                                                                                                            AACAATGGGCTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAAACGCTCACTTGTTTCCGGATTTTTTTATGCACATTGGACAGGCTCTTGATCTGGTATCCCGTTACGATTCTCTGCGTAACCC           >  2:122461/1‑150                                                                                                                                                    GCTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAAACGCTCACTTGTTTCCGGATTTTTTTATGCACATTGGACAGGCTCTTGATCTGGTATCCCGTTACGATTCTCTGCGTAACCCACTGACTTC  <  1:67679/151‑1                                                                                                                                                                                                                                                                                                            AGCGCGTGAGCATGGCGACCTGAAAGAAAACGCCGAATACCACGCAGCTCGTGAACAGCAGGGTTTCTGCGAAGGCCGTATTAAAGACATCGAAGCCAAGCTGTCGAACGCGCAGGTGATTGATGTCACCAAAATGCCCAACAATGGGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/3328611‑3328463 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAAACGCTCACTTGTTTCCGGATTTTTTTATGCACATTGGACAGGCTCTTGATCTGGTATCCCGTTACGATTCTCTGCGTAACCCACTGACTTC  <  NC_000913/4503515‑4503367

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.