Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 2,312,877 IS5 (–) +4 bp intergenic (‑128/‑608) ompC ← / → rcsD outer membrane porin protein C/phosphotransfer intermediate protein in two‑component regulatory system with RcsBC

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 2290113NA (NA)52 (0.780) 47/298 0.7 100% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 2312877 = 0 (0.000)intergenic (‑128/‑611) ompC/rcsD outer membrane porin protein C/phosphotransfer intermediate protein in two‑component regulatory system with RcsBC
* ? NC_000913 = 23128800 (0.000)72 (1.080) 57/298 0.3 100% intergenic (‑131/‑608) ompC/rcsD outer membrane porin protein C/phosphotransfer intermediate protein in two‑component regulatory system with RcsBC
?NC_000913 = 3131340 NA (NA)noncoding (1195/1195 nt) IS5 repeat region

GCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/2289965‑2290113
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTAAAACTTTTAAGATGTTTCATTTATCGCTATAGATGTTTCAAAATGTAAATGCAAGGGAACTTTTTAAGATTATTGCGGAATGGCGAAATAAGCACCTAACATCAAGCAATAATAATTCAAGGTTAAAATCAATAACTTATTCT  >  NC_000913/2312877‑2313022
                                                                                                                                                                                                                                                                                                       
GCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCC                                                                                                                                                   <  1:325652/150‑1
   GGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAAA                                                                                                                                               <  2:65634/151‑1
         GAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAAAACTTTT                                                                                                                                         <  2:685116/151‑1
         GAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAAAACTTTT                                                                                                                                         <  1:588852/151‑1
            AATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAAAACTTTTAAG                                                                                                                                      <  2:698589/151‑1
                 CTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAAAACTTTTAAGA                                                                                                                                     <  2:653004/147‑1
                    TTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAAAACTTTTAAGATGTTTC                                                                                                                               <  2:505984/150‑1
                     TCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAAAACTTTTAAGATGTTTCA                                                                                                                              <  1:1011365/150‑1
                      CTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAAAACTTTTAAGATGTTTCATT                                                                                                                            >  2:865345/1‑151
                        GGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAAAACTTTTAAGATGTTTCATTTA                                                                                                                          <  2:342240/151‑1
                           CTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAAAACTTTTAAGATGTTTCATTTATC                                                                                                                        >  2:630933/1‑150
                             GACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAAAACTTTTAAGATGTTTCATTTATCGCT                                                                                                                     <  1:563045/151‑1
                              ACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAAAACTTTTAAGATGTTTCATTTATCGCTA                                                                                                                    <  2:496003/151‑1
                                 GCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAAAACTTTTAAGATGTTTCATTTATCGCTATAG                                                                                                                 <  2:872131/151‑1
                                  CGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAAAACTTTTAAGATGTTTCATTTATCGCTATAGA                                                                                                                <  1:244812/151‑1
                                     TTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAAAACTTTTAAGATGTTTCATTTATCGCTATAGATGT                                                                                                             >  2:1004889/1‑151
                                          GCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAAAACTTTTAAGATGTTTCATTTATCGCTATAGATGTTTC                                                                                                          <  2:453644/149‑1
                                             GAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAAAACTTTTAAGATGTTTCATTTATCGCTATAGATGTTTCAAAAT                                                                                                     >  2:136149/1‑151
                                               ATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAAAACTTTTAAGATGTTTCATTTATCGCTATAGATGTTTCAAAATGT                                                                                                   <  2:289040/151‑1
                                                 TCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAAAACTTTTAAGATGTTTCATTTATCGCTATAGATGTTTCAAAATGTA                                                                                                  <  2:724766/150‑1
                                                      GTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAAAACTTTTAAGATGTTTCATTTATCGCTATAGATGTTTCAAAATGTAAATG                                                                                              <  1:450566/149‑1
                                                        CGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAAAACTTTTAAGATGTTTCATTTATCGCTATAGATGTTTCAAAATGTAAATGCAAG                                                                                          <  1:897257/151‑1
                                                           CGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAAAACTTTTAAGATGTTTCATTTATCGCTATAGATGTTTCAAAATGTAAATGCAAGGG                                                                                        >  2:79331/1‑150
                                                                GTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAAAACTTTTAAGATGTTTCATTTATCGCTATAGATGTTTCAAAATGTAAATGCAAGGGAACTTT                                                                                  <  1:807052/151‑1
                                                                              CATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAAAACTTTTAAGATGTTTCATTTATCGCTATAGATGTTTCAAAATGTAAATGCAAGGGAACTTTTTAAGATTATTGCG                                                                    >  2:676560/1‑151
                                                                                TGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAAAACTTTTAAGATGTTTCATTTATCGCTATAGATGTTTCAAAATGTAAATGCAAGGGAACTTTTTAAGATTATTGCGG                                                                   >  1:64135/1‑150
                                                                                  ATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAAAACTTTTAAGATGTTTCATTTATCGCTATAGATGTTTCAAAATGTAAATGCAAGGGAACTTTTTAAGATTATTGCGGAAT                                                                >  1:971019/1‑151
                                                                                     AACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAAAACTTTTAAGATGTTTCATTTATCGCTATAGATGTTTCAAAATGTAAATGCAAGGGAACTTTTTAAGATTATTGCGGAATGGC                                                             >  2:827583/1‑151
                                                                                      ACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAAAACTTTTAAGATGTTTCATTTATCGCTATAGATGTTTCAAAATGTAAATGCAAGGGAACTTTTTAAGATTATTGCGGAATGGCG                                                            <  2:354316/151‑1
                                                                                          TGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAAAACTTTTAAGATGTTTCATTTATCGCTATAGATGTTTCAAAATGTAAATGCAAGGGAACTTTTTAAGATTATTGCGGAATGGCGAAAT                                                        >  2:250628/1‑151
                                                                                           GTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAAAACTTTTAAGATGTTTCATTTATCGCTATAGATGTTTCAAAATGTAAATGCAAGGGAACTTTTTAAGATTATTGCGGAATGGCGAAAT                                                        <  2:419760/150‑1
                                                                                            TTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAAAACTTTTAAGATGTTTCATTTATCGCTATAGATGTTTCAAAATGTAAATGCAAGGGAACTTTTTAAGATTATTGCGGAATGGCGAAATA                                                       >  1:927107/1‑150
                                                                                                ATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAAAACTTTTAAGATGTTTCATTTATCGCTATAGATGTTTCAAAATGTAAATGCAAGGGAACTTTTTAAGATTATTGCGGAATGGCGAAATAAGC                                                    <  1:129561/149‑1
                                                                                                ATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAAAACTTTTAAGATGTTTCATTTATCGCTATAGATGTTTCAAAATGTAAATGCAAGGGAACTTTTTAAGATTATTGCGGAATGGCGAAATAAGC                                                    <  1:382195/149‑1
                                                                                                 TGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAAAACTTTTAAGATGTTTCATTTATCGCTATAGATGTTTCAAAATGTAAATGCAAGGGAACTTTTTAAGATTATTGCGGAATGGCGAAATAAGCACC                                                 >  2:110775/1‑151
                                                                                                   GCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAAAACTTTTAAGATGTTTCATTTATCGCTATAGATGTTTCAAAATGTAAATGCAAGGGAACTTTTTAAGATTATTGCGGAATGGCGAAATAAGCACCT                                                >  2:705931/1‑150
                                                                                                   GCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAAAACTTTTAAGATGTTTCATTTATCGCTATAGATGTTTCAAAATGTAAATGCAAGGGAACTTTTTAAGATTATTGCGGAATGGCGAAATAAGCACCT                                                >  1:621824/1‑150
                                                                                                      CCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAAAACTTTTAAGATGTTTCATTTATCGCTATAGATGTTTCAAAATGTAAATGCAAGGGAACTTTTTAAGATTATTGCGGAATGGCGAAATAAGCACCTAA                                              <  2:736430/149‑1
                                                                                                        AGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAAAACTTTTAAGATGTTTCATTTATCGCTATAGATGTTTCAAAATGTAAATGCAAGGGAACTTTTTAAGATTATTGCGGAATGGCGAAATAAGCACCTAACATC                                          >  2:41410/1‑151
                                                                                                          ATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAAAACTTTTAAGATGTTTCATTTATCGCTATAGATGTTTCAAAATGTAAATGCAAGGGAACTTTTTAAGATTATTGCGGAATGGCGAAATAAGCACCTAACATCAA                                        <  1:286808/151‑1
                                                                                                          ATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAAAACTTTTAAGATGTTTCATTTATCGCTATAGATGTTTCAAAATGTAAATGCAAGGGAACTTTTTAAGATTATTGCGGAATGGCGAAATAAGCACCTAACATCAA                                        <  2:802612/151‑1
                                                                                                            GACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAAAACTTTTAAGATGTTTCATTTATCGCTATAGATGTTTCAAAATGTAAATGCAAGGGAACTTTTTAAGATTATTGCGGAATGGCGAAATAAGCACCTAACATCAAG                                       <  2:393067/150‑1
                                                                                                                     GATCTCATATCAGGGACTTGTTCGCACCTTCCCTAAAACTTTTAAGATGTTTCATTTATCGCTATAGATGTTTCAAAATGTAAATGCAAGGGAACTTTTTAAGATTATTGCGGAATGGCGAAATAAGCACCTAACATCAAGCAATAATAA                              <  1:83054/150‑1
                                                                                                                          CATATCAGGGACTTGTTCGCACCTTCCCTAAAACTTTTAAGATGTTTCATTTATCGCTATAGATGTTTCAAAATGTAAATGCAAGGGAACTTTTTAAGATTATTGCGGAATGGCGAAATAAGCACCTAACATCAAGCAATAATAATT                            <  1:464239/147‑1
                                                                                                                           ATATCAGGGACTTGTTCGCACCTTCCCTAAAACTTTTAAGATGTTTCATTTATCGCTATAGATGTTTCAAAATGTAAATGCAAGGGAACTTTTTAAGATTATTGCGGAATGGCGAAATAAGCACCTAACATCAAGCAATAATAATTCAAGG                       >  1:275679/1‑151
                                                                                                                              TCAGGGACTTGTTCGCACCTTCCCTAAAACTTTTAAGATGTTTCATTTATCGCTATAGATGTTTCAAAATGTAAATGCAAGGGAACTTTTTAAGATTATTGCGGAATGGCGAAATAAGCACCTAACATCAAGCAATAATAATTCAAGGTT                     >  2:897071/1‑150
                                                                                                                               CAGGGACTTGTTCGCACCTTCCCTAAAACTTTTAAGATGTTTCATTTATCGCTATAGATGTTTCAAAATGTAAATGCAAGGGAACTTTTTAAGATTATTGCGGAATGGCGAAATAAGCACCTAACATCAAGCAATAATAATTCAAGGTTA                    >  2:546871/1‑150
                                                                                                                               CAGGGACTTGTTCGCACCTTCCCTAAAACTTTTAAGATGTTTCATTTATCGCTATAGATGTTTCAAAATGTAAATGCAAGGGAACTTTTTAAGATTATTGCGGAATGGCGAAATAAGCACCTAACATCAAGCAATAATAATTCAAGGTTA                    >  2:340497/1‑150
                                                                                                                                AGGGACTTGTTCGCACCTTCCCTAAAACTTTTAAGATGTTTCATTTATCGCTATAGATGTTTCAAAATGTAAATGCAAGGGAACTTTTTAAGATTATTGCGGAATGGCGAAATAAGCACCTAACATCAAGCAATAATAATTCAAGGTTAAA                  <  1:39316/151‑1
                                                                                                                                AGGGACTTGTTCGCACCTTCCCTAAAACTTTTAAGATGTTTCATTTATCGCTATAGATGTTTCAAAATGTAAATGCAAGGGAACTTTTTAAGATTATTGCGGAATGGCGAAATAAGCACCTAACATCAAGCAATAATAATTCAAGGTTAA                   >  1:856266/1‑150
                                                                                                                                                CTTCCCTAAAACTTTTAAGATGTTTCATTTATCGCTATAGATGTTTCAAAATGTAAATGCAAGGGAACTTTTTAAGATTATTGCGGAATGGCGAAATAAGCACCTAACATCAAGCAATAATAATTCAAGGTTAAAATCAATAACTTATTCT  >  1:673382/1‑151
                                                                                                                                                  TCCCTAAAACTTTTAAGATGTTTCATTTATCGCTATAGATGTTTCAAAATGTAAATGCAAGGGAACTTTTTAAGATTATTGCGGAATGGCGAAATAAGCACCTAACATCAAGCAATAATAATTCAAGGTTAAAATCAATAACTTATTCT  <  1:79332/149‑1
                                                                                                                                                                                                                                                                                                       
GCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/2289965‑2290113
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTAAAACTTTTAAGATGTTTCATTTATCGCTATAGATGTTTCAAAATGTAAATGCAAGGGAACTTTTTAAGATTATTGCGGAATGGCGAAATAAGCACCTAACATCAAGCAATAATAATTCAAGGTTAAAATCAATAACTTATTCT  >  NC_000913/2312877‑2313022

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.