Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913 1,198,498 (AATGATGA)6→5 intergenic (‑261/‑190) ymfE ← / → lit e14 prophage; putative inner membrane protein/T4 phage exclusion protein; cell death peptidase, e14 prophage

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1198454 1198461 8 48 [0] [0] 48 ymfE/lit e14 prophage; putative inner membrane protein/T4 phage exclusion protein; cell death peptidase, e14 prophage

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1198462 =0 (0.000)33 (0.940) 32/210 0.2 100% intergenic (‑225/‑233) ymfE/lit e14 prophage; putative inner membrane protein/T4 phage exclusion protein; cell death peptidase, e14 prophage
?NC_000913 = 1198453 0 (0.000)intergenic (‑216/‑242) ymfE/lit e14 prophage; putative inner membrane protein/T4 phage exclusion protein; cell death peptidase, e14 prophage

TATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGATATTCTAAACATGTCGATAATTCATTACGCAT  >  NC_000913/1198323‑1198611
                                                                                                                                           |                                                                                                                                                     
attttttgtatctgttcaggctgacctagcttcgctgacagacaatattgtgatcagtagcacgtatcgaggaggagtagcgctacaaatttgacgctgggtgagaatctgaaattgatagaaatgaaataatgaaataatgaaatgatga                                                                                                                                            >  1:611104‑M1/140‑151 (MQ=255)
  tttttgtatctgttcaggctgacctagcttcgctgacagacaatattgtgatcagtagcacgtatcgaggaggagtagcgctacaaatttgacgctgggtgagaatctgaaattgatagaaatgaaataatgaaataatgaaatgatgaa                                                                                                                                           >  1:279098‑M1/138‑150 (MQ=255)
     ttgtatctgttcaggctgacctagcttcgctgacagacaatattgtgatcagtagcacgtatcgaggaggagtagcgctacaaatttgacgctgggtgagaatctgaaattgatagaaatgaaataatgaaataatgaaatgatgaaatg                                                                                                                                        <  1:744969‑M1/16‑1 (MQ=255)
     ttgtatctgttcaggctgacctagcttcgctgacagacaatattgtgatcagtagcacgtatcgaggaggagtagcgctacaaatttgacgctgggtgagaatctgaaattgatagaaatgaaataatgaaataatgaaatgatgaaatg                                                                                                                                        <  1:709800‑M1/16‑1 (MQ=255)
       gtatctgttcaggctgacctagcttcgctgacagacaatattgtgatcagtagcacgtatcgaggaggagtagcgctacaaatttgacgctgggtgagaatctgaaattgatagaaatgaaataatgaaataatgaaatgatgaaatgat                                                                                                                                      >  2:127518‑M1/133‑150 (MQ=255)
       gtatctgttcaggctgacctagcttcgctgacagacaatattgtgatcagtagcacgtatcgaggaggagtagcgctacaaatttgacgctgggtgagaatctgaaattgatagaaatgaaataatgaaataatgaaatgatgaaatgat                                                                                                                                      >  1:456354‑M1/133‑150 (MQ=255)
             gttcaggctgacctagcttctctgacagacaatattgtgatcagtagcacgtatcgaggaggagtagcgctacaaatttgacgctgggtgagaatctgaaattgatagaaatgaaataatgaaataatgaaatgatgaaatgatgaa                                                                                                                                   >  1:729760‑M1/127‑147 (MQ=255)
             gttcaggctgacctagcttcgctgacagacaatattgtgatcagtagcacgtatcgaggaggagtagcgctacaaatttgacgctgggtgagaatctgaaattgatagaaatgaaataatgaaataatgaaatgatgaaatgatgaaatg                                                                                                                                >  2:371505‑M1/127‑150 (MQ=255)
               tcaggctgacctagcttcgctgacagacaatattgtgatcagtagcacgtatcgaggaggagtagcgctacaaatttgacgctgggtgagaatctgaaattgatagaaatgaaataatgaaataatgaaatgatgaaatgatgaaatgat                                                                                                                              >  1:194491‑M1/125‑150 (MQ=255)
                caggctgacctagcttcgctgacagacaatattgtgatcagtagcacgtatcgaggaggagtagcgctacaaatttgacgctgggtgagaatctgaaattgatagaaatgaaataatgaaataatgaaatgatgaaatgatgaaatgatga                                                                                                                            >  2:348503‑M1/124‑151 (MQ=255)
                     tgacctagcttcgctgacagacaatattgtgatcagtagcacgtatcgaggaggagtagcgctacaaatttgacgctgggtgagaatctgaaattgatagaaatgaaataatgaaataatgaaatgatgaaatgatgaaatgatgaaatg                                                                                                                        <  2:227901‑M1/32‑1 (MQ=255)
                      gacctagcttcgctgacagacaatattgtgatcagtagcacgtatcgaggaggagtagcgctacaaatttgacgctgggtgagaatctgaaattgatagaaatgaaataatgaaataatgaaatgatgaaatgatgaaatgatgaaatgat                                                                                                                      >  2:704826‑M1/118‑151 (MQ=255)
                       acctagcttcgctgacagacaatattgtgatcagtagcacgtatcgaggaggagtagcgctacaaatttgacgctgggggagaatctgaaattgatagaaatgaaataatgaaataatgaaatgatgaaatgatgaaatgatgaaatgatg                                                                                                                     <  1:379090‑M1/35‑1 (MQ=255)
                           agcttcgctgacagacaatattgtgatcagtagcacgtatcgaggaggagtagcgctacaaatttgacgctgggtgagaatctgaaattgatagaaatgaaataatgaaataatgaaatgatgaaatgatgaaatgatgaaatgatgaaa                                                                                                                  >  1:650355‑M1/113‑150 (MQ=255)
                             cttcgctgacagacaatattgtgatcagtagcacgtatcgaggaggagtagcgctacaaatttgacgctgggtgagaatctgaaattgatagaaatgaaataatgaaataatgaaatgatgaaatgatgaaatgatgaaatgatgaaatg                                                                                                                <  1:116644‑M1/40‑1 (MQ=255)
                                 gctgacagacaatattgtgatcagtagcacgtatcgaggaggagtagcgctacaaatttgacgctgggtgagaatctgaaattgatagaaatgaaataatgaaataATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAc                                                                                                           >  2:676559‑M1/107‑151 (MQ=255)
                                   tgacagacaatattgtgatcagtagcacgtatcgaggaggagtagcgctacaaatttgacgctgggtgagaatccgaaattgatagaaatgaaataatgaaataATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAc                                                                                                           <  2:125160‑M1/45‑1 (MQ=255)
                                       agacaatattgtgatcagtagcacgtatcgaggaggagtagcgctacaaatttgacgctgggtgagaatctgaaattgatagaaatgaaataatgaaataATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAgtg                                                                                                     >  2:189219‑M1/101‑151 (MQ=255)
                                       agacaatattgtgatcagtagcacgtatcgaggaggagtagcgctacaaatttgacgctgggtgagaatctgaaattgatagaaatgaaataatgaaataATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAgtg                                                                                                     >  2:384009‑M1/101‑151 (MQ=255)
                                           aatattgtgatcagtagcacgtatcgaggaggagtagcgctacaaatttgacgctgggtgagaatctgaaattgatagaaatgaaataatgaaataATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCa                                                                                                 >  2:138637‑M1/97‑151 (MQ=255)
                                              attgtgatcagtagcacgtatcgaggaggagtagcgctacaaatttgacgctgggtgagaatctgaaattgatagaaatgaaataatgaaataATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGt                                                                                               >  2:17885‑M1/94‑150 (MQ=255)
                                                tgtgatcagtagcacgtatcgaggaggagtagcgctacaaatttgacgctgggtgagaatctgaaattgatagaaatgaaataatgaaataATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTgg                                                                                             >  1:547474‑M1/92‑150 (MQ=255)
                                                 gtgatcagtagcacgtatcgaggaggagtagcgctacaaatttgacgctgggtgagaatctgaaattgatagaaatgaaataatgaaataATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGc                                                                                           <  1:647004‑M1/61‑1 (MQ=255)
                                                   gatcagtagcacgtatcgaggaggagtagcgctacaaatttgacgctgggtgagaatctgaaattgatagaaatgaaataatgaaataATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCAc                                                                                         >  2:572134‑M1/89‑151 (MQ=255)
                                                        gtagcacgtatcgaggaggagtagcgctacaaatttgacgctgggtgagaatctgaaattgatagaaatgaaataatgaaataATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATg                                                                                    <  1:311953‑M1/68‑1 (MQ=255)
                                                            cacgtatcgaggaggagtagcgctacaaatttgacgctgggtgagaatctgaaattgatagaaatgaaataatgaaataATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGgtgt                                                                                >  1:133702‑M1/80‑151 (MQ=255)
                                                                     aggaggagtagcgctacaaatttgacgctgggtgagaatctgaaattgatagaaatgaaataatgaaataATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACa                                                                       >  1:402739‑M1/71‑151 (MQ=255)
                                                                       gaggagtagcgctacaaatttgacgctgggtgagaatctgaaattgatagaaatgaaataatgaaataATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATg                                                                     >  1:540593‑M1/69‑151 (MQ=255)
                                                                       gaggagtagcgctacaaatttgacgctgggtgagaatctgaaattgatagaaatgaaataatgaaataATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATg                                                                     <  2:652099‑M1/83‑1 (MQ=255)
                                                                        aggagtagcgctacaaatttgacgctgggtgagaatctgaaattgatagaaatgaaataatgaaataATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGc                                                                    <  1:726633‑M1/84‑1 (MQ=255)
                                                                              agcgctacaaatttgacgctgggtgagaatctgaaattgatagaaatgaaataatgaaataATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCt                                                              >  2:501471‑M1/62‑151 (MQ=255)
                                                                                    acaaatttgacgctgggtgagaatctgaaattgatagaaatgaaataatgaaataATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCg                                                        <  2:440747‑M1/96‑1 (MQ=255)
                                                                                     caaatttgacgctgggtgagaatctgaaattgatagaaatgaaataatgaaataATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGt                                                       >  2:744839‑M1/55‑151 (MQ=255)
                                                                                       aatttgacgctgggtgagaatctgaaattgatagaaatgaaataatgaaataATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTc                                                     >  2:41843‑M1/53‑151 (MQ=255)
                                                                                             acgctgggtgagaatctgaaattgatagaaatgaaataatgaaataATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAgt                                                >  1:461918‑M1/47‑150 (MQ=255)
                                                                                                    gtgagaatctgaaattgatagaaatgaaataatgaaataATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGGGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCtat                                        <  1:110696‑M1/112‑1 (MQ=255)
                                                                                                        gaatctgaaattgatagaaatgaaataatgaaataATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCa                                    >  2:709739‑M1/36‑151 (MQ=255)
                                                                                                              gaaattgatagaaatgaaataatgaaataATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGat                                 >  2:464082‑M1/30‑148 (MQ=255)
                                                                                                                aattgatagaaatgaaataatgaaataATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGATATTCt                            <  2:662374‑M1/124‑1 (MQ=255)
                                                                                                                     atagaaatgaaataatgaaataATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGATATTCTAAAc                        <  2:33702‑M1/128‑1 (MQ=255)
                                                                                                                       agaaatgaaataatgaaataATGAATTGATGAAATGATGCAATGATGAAATGATGAAATGATGACAGAGAGTCCAGTGGGCACGGATGGTGTCTTACGAAATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGATATTCTAAACATg                     <  2:639688‑M1/131‑1 (MQ=255)
                                                                                                                       agaaatgaaataatgaaataATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGATATTCTAAACATg                     >  2:311744‑M1/21‑151 (MQ=255)
                                                                                                                         aaatgaaataatgaaataATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGATATTCTAAACATGTc                   >  2:443671‑M1/19‑151 (MQ=255)
                                                                                                                             gaaataatgaaataATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGATATTCTAAACATGTCGAt                <  1:592630‑M1/136‑1 (MQ=255)
                                                                                                                                      aaataATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGATATTCTAAACATGTCGATAATTCATTAc      >  2:272915‑M1/6‑151 (MQ=255)
                                                                                                                                        ataATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCATTCCCAGTGTGCCTATAGCAGATATTCTAAACATGTCGATAATTCATTACGc    <  2:275371‑M1/148‑1 (MQ=255)
                                                                                                                                         taATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGATATTCTAAACATGTCGATAATTCATTACGc    <  2:607335‑M1/148‑1 (MQ=255)
                                                                                                                                          aATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGATATTCTAAACATGTCGATAATTCATTACGCat  <  1:135301‑M1/150‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                                     
TATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGATATTCTAAACATGTCGATAATTCATTACGCAT  >  NC_000913/1198323‑1198611

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.