Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	1,198,498	(AATGATGA)6→5	intergenic (‑261/‑190)	ymfE ← / → lit	e14 prophage; putative inner membrane protein/T4 phage exclusion protein; cell death peptidase, e14 prophage

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	1198454	1198461	8	48 [0]	[0] 48	ymfE/lit	e14 prophage; putative inner membrane protein/T4 phage exclusion protein; cell death peptidase, e14 prophage

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1198462 =	0 (0.000)	33 (0.940)	32/210	0.2	100%	intergenic (‑225/‑233)	ymfE/lit	e14 prophage; putative inner membrane protein/T4 phage exclusion protein; cell death peptidase, e14 prophage
	?	NC_000913	= 1198453	0 (0.000)					intergenic (‑216/‑242)	ymfE/lit	e14 prophage; putative inner membrane protein/T4 phage exclusion protein; cell death peptidase, e14 prophage

ATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGATATTCTAAACATGTCGATAATTCA  >  NC_000913/1198315‑1198603                                                                                                                                           |                                                                                                                                                       aTTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatga                                                                                                                                            >  1:611104‑M2/1‑139 (MQ=255)   tttttGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaa                                                                                                                                           >  1:279098‑M2/1‑137 (MQ=255)      ttGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatg                                                                                                                                        <  1:744969‑M2/150‑17 (MQ=255)      ttGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatg                                                                                                                                        <  1:709800‑M2/150‑17 (MQ=255)        gTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgat                                                                                                                                      >  2:127518‑M2/1‑132 (MQ=255)        gTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgat                                                                                                                                      >  1:456354‑M2/1‑132 (MQ=255)              gTTCAGGCTGACCTAGCTTCTCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaa                                                                                                                                   >  1:729760‑M2/1‑126 (MQ=255)              gTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatg                                                                                                                                >  2:371505‑M2/1‑126 (MQ=255)                tCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgat                                                                                                                              >  1:194491‑M2/1‑124 (MQ=255)                 cAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatga                                                                                                                            >  2:348503‑M2/1‑123 (MQ=255)                      tGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatg                                                                                                                        <  2:227901‑M2/150‑33 (MQ=255)                       gACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgat                                                                                                                      >  2:704826‑M2/1‑117 (MQ=255)                        aCCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGGGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatg                                                                                                                     <  1:379090‑M2/151‑36 (MQ=255)                            aGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaaa                                                                                                                  >  1:650355‑M2/1‑112 (MQ=255)                              cTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaaatg                                                                                                                <  1:116644‑M2/150‑41 (MQ=255)                                  gCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgac                                                                                                           >  2:676559‑M2/1‑106 (MQ=255)                                    tGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCCGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgac                                                                                                           <  2:125160‑M2/149‑46 (MQ=255)                                        agacaATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgacagagtg                                                                                                     >  2:189219‑M2/1‑100 (MQ=255)                                        agacaATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgacagagtg                                                                                                     >  2:384009‑M2/1‑100 (MQ=255)                                            aaTATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgacagagtgtcca                                                                                                 >  2:138637‑M2/1‑96 (MQ=255)                                               atTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgacagagtgtccagt                                                                                               >  2:17885‑M2/1‑93 (MQ=255)                                                 tgtgATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgacagagtgtccagtgg                                                                                             >  1:547474‑M2/1‑91 (MQ=255)                                                  gtgATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgacagagtgtccagtgggc                                                                                           <  1:647004‑M2/151‑62 (MQ=255)                                                    gATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgacagagtgtccagtgggcac                                                                                         >  2:572134‑M2/1‑88 (MQ=255)                                                         gTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgacagagtgtccagtgggcacggatg                                                                                    <  1:311953‑M2/151‑69 (MQ=255)                                                             cACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgacagagtgtccagtgggcacggatggtgt                                                                                >  1:133702‑M2/1‑79 (MQ=255)                                                                      aggaggagTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgacagagtgtccagtgggcacggatggtgtcttacgaca                                                                       >  1:402739‑M2/1‑70 (MQ=255)                                                                        gaggagTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgacagagtgtccagtgggcacggatggtgtcttacgacatg                                                                     >  1:540593‑M2/1‑68 (MQ=255)                                                                        gaggagTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgacagagtgtccagtgggcacggatggtgtcttacgacatg                                                                     <  2:652099‑M2/151‑84 (MQ=255)                                                                         aggagTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgacagagtgtccagtgggcacggatggtgtcttacgacatgc                                                                    <  1:726633‑M2/151‑85 (MQ=255)                                                                               aGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgacagagtgtccagtgggcacggatggtgtcttacgacatgcttacct                                                              >  2:501471‑M2/1‑61 (MQ=255)                                                                                     aCAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgacagagtgtccagtgggcacggatggtgtcttacgacatgcttaccttaatcg                                                        <  2:440747‑M2/151‑97 (MQ=255)                                                                                      cAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgacagagtgtccagtgggcacggatggtgtcttacgacatgcttaccttaatcgt                                                       >  2:744839‑M2/1‑54 (MQ=255)                                                                                        aaTTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgacagagtgtccagtgggcacggatggtgtcttacgacatgcttaccttaatcgttc                                                     >  2:41843‑M2/1‑52 (MQ=255)                                                                                              aCGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgacagagtgtccagtgggcacggatggtgtcttacgacatgcttaccttaatcgttcccagt                                                >  1:461918‑M2/1‑46 (MQ=255)                                                                                                     gTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgacagagtgtccagtgggcacggatggggtcttacgacatgcttaccttaatcgttcccagtgtgcctat                                        <  1:110696‑M2/151‑113 (MQ=255)                                                                                                         gaATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgacagagtgtccagtgggcacggatggtgtcttacgacatgcttaccttaatcgttcccagtgtgcctatagca                                    >  2:709739‑M2/1‑35 (MQ=255)                                                                                                               gAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgacagagtgtccagtgggcacggatggtgtcttacgacatgcttaccttaatcgttcccagtgtgcctatagcagat                                 >  2:464082‑M2/1‑29 (MQ=255)                                                                                                                 aaTTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgacagagtgtccagtgggcacggatggtgtcttacgacatgcttaccttaatcgttcccagtgtgcctatagcagatattct                            <  2:662374‑M2/151‑125 (MQ=255)                                                                                                                      aTAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgacagagtgtccagtgggcacggatggtgtcttacgacatgcttaccttaatcgttcccagtgtgcctatagcagatattctaaac                        <  2:33702‑M2/150‑129 (MQ=255)                                                                                                                        aGAAATGAAATAATGAAATAatgaattgatgaaatgatgcaatgatgaaatgatgaaatgatgacagagagtccagtgggcacggatggtgtcttacgaaatgcttaccttaatcgttcccagtgtgcctatagcagatattctaaacatg                     <  2:639688‑M2/151‑132 (MQ=255)                                                                                                                        aGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgacagagtgtccagtgggcacggatggtgtcttacgacatgcttaccttaatcgttcccagtgtgcctatagcagatattctaaacatg                     >  2:311744‑M2/1‑20 (MQ=255)                                                                                                                          aaatgaaatAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgacagagtgtccagtgggcacggatggtgtcttacgacatgcttaccttaatcgttcccagtgtgcctatagcagatattctaaacatgtc                   >  2:443671‑M2/1‑18 (MQ=255)                                                                                                                              gaaataatgaaataatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgacagagtgtccagtgggcacggatggtgtcttacgacatgcttaccttaatcgttcccagtgtgcctatagcagatattctaaacatgtcgat                <  1:592630‑M2/150‑137 (MQ=255)                                                                                                                                       aaataatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgacagagtgtccagtgggcacggatggtgtcttacgacatgcttaccttaatcgttcccagtgtgcctatagcagatattctaaacatgtcgataattcattac      >  2:272915‑M2/1‑5 (MQ=255)                                                                                                                                         ataatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgacagagtgtccagtgggcacggatggtgtcttacgacatgcttaccttaatcattcccagtgtgcctatagcagatattctaaacatgtcgataattcattacgc    <  2:275371‑M2/151‑149 (MQ=255)                                                                                                                                          taatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgacagagtgtccagtgggcacggatggtgtcttacgacatgcttaccttaatcgttcccagtgtgcctatagcagatattctaaacatgtcgataattcattacgc    <  2:607335‑M2/150‑149 (MQ=255)                                                                                                                                           aatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgacagagtgtccagtgggcacggatggtgtcttacgacatgcttaccttaatcgttcccagtgtgcctatagcagatattctaaacatgtcgataattcattacgcat  <  1:135301‑M2/151‑151 (MQ=255)                                                                                                                                           |                                                                                                                                                       ATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGATATTCTAAACATGTCGATAATTCA  >  NC_000913/1198315‑1198603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑