Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 2,628,616 IS2 (+) +5 bp coding (319‑323/2244 nt) yfgF ← cyclic‑di‑GMP phosphodiesterase, anaerobic

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 2070271NA (NA)18 (0.510) 17/292 1.3 94.6% noncoding (1/1331 nt) IS2 repeat region
?NC_000913 = 2628620 1 (0.030)coding (319/2244 nt) yfgF cyclic‑di‑GMP phosphodiesterase, anaerobic
* ? NC_000913 2628616 =1 (0.030)17 (0.490) 15/292 1.6 94.3% coding (323/2244 nt) yfgF cyclic‑di‑GMP phosphodiesterase, anaerobic
?NC_000913 2996361 = NA (NA)noncoding (1331/1331 nt) IS2 repeat region

TTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGTAGTGAAAGGTCCATATTCCGTACTGT  >  NC_000913/2070129‑2070421
                                                                                                                                              |                                                                                                                                                      
tttCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCg                                                                                                                                                  <  1:2904061/149‑1 (MQ=32)
  tCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAg                                                                                                                                               <  2:1005088/150‑1 (MQ=34)
  tCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAg                                                                                                                                               <  1:2168828/150‑1 (MQ=34)
   ccTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGc                                                                                                                                              <  2:3990891/150‑1 (MQ=35)
    gggtgggGTACGCCGTCTGCGGTTCACCAGGGGGAAGACATCAATCATCTGTGCTCCAATGACTAGTCTAAAAAAAAAAATTAAGACTAACACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGATGCCAGTCTAGTGGCGAAGc                                                                                                                                              >  1:475124/3‑149 (MQ=11)
     ggtgtgGAAAGCGGTCTGCGTGGCTACGGCGCGACGACATCAATCATATGTTGTCGGATGCCTAGTCTTAAAACAAAAAAAAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCAtc                                                                                                                                           >  1:2920406/2‑151 (MQ=2)
             aCGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCgt                                                                                                                                    <  2:2682758/150‑1 (MQ=255)
              cGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCgtgt                                                                                                                                  <  2:2776681/151‑1 (MQ=255)
                      gCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATtc                                                                                                                          <  2:292157/151‑1 (MQ=255)
                          ttCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCAt                                                                                                                      >  1:1085502/1‑151 (MQ=255)
                              ccGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCAGTg                                                                                                                    <  1:3067396/149‑1 (MQ=255)
                                ggCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATgg                                                                                                                >  1:2484654/1‑151 (MQ=255)
                                     tAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCagg                                                                                                           >  2:3388789/1‑151 (MQ=255)
                                      aaGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCagg                                                                                                           >  2:1265291/1‑150 (MQ=255)
                                          cATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTa                                                                                                      <  1:3697779/151‑1 (MQ=255)
                                                      gTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAgcgc                                                                                          <  2:1283081/151‑1 (MQ=255)
                                                      gTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAgcgc                                                                                          <  1:2580793/151‑1 (MQ=255)
                                                                 aCTAGTCGAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATGCTGGGGTCCTTTCTAGTTGCGAAGCATCCTCCCGTGTTTTTAATCTCATGGGTGCTCACTTTGCCCCTTATCAGCGCTCCGGAGAtc                                                                                <  1:2528593/150‑2 (MQ=12)
                                                                             aCTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTggg                                                                   <  2:1444805/151‑1 (MQ=255)
                                                                                aGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGa                                                                  <  2:3751506/149‑1 (MQ=255)
                                                                                        gACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGAtg                                                         <  1:2805023/150‑2 (MQ=255)
                                                                                          cTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGGGTGGGACTGTTGAt                                                          >  1:3690550/1‑147 (MQ=255)
                                                                                            aTCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATg                                                     <  1:779636/150‑1 (MQ=255)
                                                                                               aCTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTc                                                 <  1:167009/151‑1 (MQ=255)
                                                                                                  tATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTg                                               <  2:3274804/150‑1 (MQ=255)
                                                                                                             tatTGGTTGAGTGGAGGTTCAGGGAGAAAGGGTAGTCCCGAAGGATAATCCCGGGTTGTACTTCTCATTGATGAAAAAAAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGAGGGACTGTTGATAGATGAGTCTGCGCTGGAGCg                                     >  1:1995194/1‑149 (MQ=255)
                                                                                                              atTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCgtg                                   <  1:1934893/150‑1 (MQ=255)
                                                                                                                 ggTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTg                                >  2:2365347/1‑150 (MQ=255)
                                                                                                                 ggTTGTATGGAGAGTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGCGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTg                                >  1:454581/1‑150 (MQ=255)
                                                                                                                    tGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGt                            >  1:2584883/1‑151 (MQ=255)
                                                                                                                         ggAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGTAGt                         <  1:2645593/149‑1 (MQ=255)
                                                                                                                           agaTTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGTAGTGaaa                     <  1:1125157/151‑1 (MQ=255)
                                                                                                                             aTTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGTAGTGAAAgg                   <  1:3335685/151‑1 (MQ=255)
                                                                                                                               tCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGTAGTGAAAGGTc                 >  2:3384048/1‑151 (MQ=255)
                                                                                                                                     ggCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGTAGTGAAAGGTCCATAtt           <  2:2205691/151‑1 (MQ=255)
                                                                                                                                       ccAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGTAGTGAAAGGTCCATATTcc         <  2:415336/151‑1 (MQ=255)
                                                                                                                                         tgTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGTAGTGAAAGGTCCATATTCCGt       >  1:3208469/2‑151 (MQ=255)
                                                                                                                                         aGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGTAGTGAAAGGTCCATATTCCGt       <  2:350213/151‑1 (MQ=255)
                                                                                                                                              aGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGTAGTGAAAGGTCCATATTCCGTACTGt  <  1:3235352/151‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                      
TTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGTAGTGAAAGGTCCATATTCCGTACTGT  >  NC_000913/2070129‑2070421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.