Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 2,628,616 IS2 (+) +5 bp coding (319‑323/2244 nt) yfgF ← cyclic‑di‑GMP phosphodiesterase, anaerobic

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 2070271NA (NA)18 (0.510) 17/292 1.3 94.6% noncoding (1/1331 nt) IS2 repeat region
?NC_000913 = 2628620 1 (0.030)coding (319/2244 nt) yfgF cyclic‑di‑GMP phosphodiesterase, anaerobic
* ? NC_000913 2628616 =1 (0.030)17 (0.490) 15/292 1.6 94.3% coding (323/2244 nt) yfgF cyclic‑di‑GMP phosphodiesterase, anaerobic
?NC_000913 2996361 = NA (NA)noncoding (1331/1331 nt) IS2 repeat region

ACACCGACCAGATTTTCTCTGCTCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCGCCCAGCTGAAAA  >  NC_000913/2628466‑2628763
                                                                                                                                                      |                                                                                                                                                   
agcaaagaaccttgcagaggcgttcgagcattataacgaatggcatccgcatagtgcgctgggttatcgctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccag                                                                                                                                                     >  2:3992268‑M1/151‑151 (MQ=255)
   ccGACCAGATTTTCTCTGCTCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCgggg                                                                                                                                                    >  2:2250582/1‑149 (MQ=255)
      gaaccttgcagaggcgttcgagcattataacgaatggcatccgcatagtgcgctgggttatcgctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGGAGTaa                                                                                                                                               >  1:178510‑M1/145‑151 (MQ=255)
        accttgcagaggcgttcgagcattataacgaatggcatccgcatagtgcgctgggttatcgctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGGAGTAAAg                                                                                                                                             >  1:3396670‑M1/143‑151 (MQ=255)
         ccttgcagaggcgttcgagcattataacgaatggcatccgcatagtgcgctgggttatcgctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGGAGTAAAGa                                                                                                                                            >  1:233176‑M1/142‑151 (MQ=255)
                   gcgttcgagcattataacgaatggcatccgcatagtgcgctgggttatcgctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGGAGTAAAGACCCTGTAAcc                                                                                                                                  >  1:899404‑M1/132‑151 (MQ=255)
                   gcgttcgagcattataacgaatggcatccgcatagtgcgctgggttatcgctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGGAGTAAAGACCCTGTAAcc                                                                                                                                  <  1:3160638‑M1/20‑1 (MQ=255)
                    cgttcgagcattataacgaatggcatccgcatagtgcgctgggttatcgctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGGAGTAAAGACCCTGTAACCg                                                                                                                                 >  1:3172503‑M1/131‑151 (MQ=255)
                        gCCAGTAATCCTACAAATGCAGCAAACTGGATAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTtagactggccccctgaatct                                                                                                                             >  2:1800265‑M2/1‑131 (MQ=255)
                                     cAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTtagactggccccctgaatctccagacaaccaat                                                                                                                >  1:352516‑M2/1‑118 (MQ=255)
                                              ccgcatagtgcgctgggttatcgctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATgataatgataat                                                                                                       <  2:2180128‑M1/47‑1 (MQ=255)
                                              caAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTtagactggccccctgaatctccagacaaccaatatcacttaa                                                                                                       >  1:2830515‑M2/1‑109 (MQ=255)
                                                    ggAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTtagactggccccctgaatctccagacaaccaatatcacttaaataag                                                                                                  <  1:1306542‑M2/150‑48 (MQ=255)
                                                      aaaaGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTtagactggccccctgaatctccagacaaccaatatcacttaaataagtg                                                                                                <  1:3272855‑M2/150‑50 (MQ=255)
                                                      aaaaGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTtagactggccccctgaatctccagacaaccaatatcacttaaataagtg                                                                                                <  1:1649962‑M2/150‑50 (MQ=255)
                                                                 atcgccccccacggaaatacctgcggcagcgggcttgtaatgggtaaagtgataaaagatgtctgaaaatataggggcaaatccaGGAGAAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAAt                                                                                       >  2:2753469‑M1/86‑148 (MQ=255)
                                                                            cAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagttttt                                                                           <  2:2401966‑M2/149‑71 (MQ=255)
                                                                             gggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTa                                                                         <  2:2211736‑M1/77‑1 (MQ=255)
                                                                                     cggcggcagggggcttgtaatgggttaagtgataacagatgtctggaaatataggggaaaaccaaGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCa                                                                 >  2:1691181‑M1/66‑150 (MQ=255)
                                                                                        tCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttagactagtcattgg                                                             <  2:2109977‑M2/151‑85 (MQ=255)
                                                                                         ggcagcgggtttgtaatgggttaagtgataaaagatgtcgggaaatataggggaaaatccgGGATTAAAGCCCTTTTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGctc                                                             >  2:1789729‑M1/62‑150 (MQ=255)
                                                                                         ggcagcgggtttgtaaggggtaaagagataacagatgtcgggaaatatagggaaaaatccaGGAGAAAAGACCTTTTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGctct                                                            >  1:2757621‑M1/62‑151 (MQ=255)
                                                                                                aaTATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttagactagtcattggagaac                                                        <  2:2824160‑M2/148‑90 (MQ=255)
                                                                                                      cGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttagactagtcattggagaacagatgattg                                               >  2:192265‑M2/1‑53 (MQ=255)
                                                                                                          ggtttaagtaataacagatgtctgaaaatataggggaaaacccaGGAGTAAAGCCCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACaaa                                           >  2:2713092‑M1/45‑151 (MQ=255)
                                                                                                          gggAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTtagactggacccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttagactagtcattggagaacagatgattgat                                             >  1:3721252‑M2/1‑49 (MQ=255)
                                                                                                            gAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttagactagtcattggagaacagatgattgatgtct                                         <  1:4101209‑M2/151‑105 (MQ=255)
                                                                                                                 gtgaaaacagatgtcgggaaatatgggggaaaatccaGAAGAAAACCCCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAAtccca                                    >  1:1488253‑M1/38‑150 (MQ=255)
                                                                                                                  aaaCGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttagactagtcattggagaacagatgattgatgtcttagggc                                   <  1:1201450‑M2/151‑111 (MQ=255)
                                                                                                                   aaCGGGTCTCACTGTGTGAAGCGTGGTTACGATGGGGAGAAAACCCCCTGTAACCGCCCCAACAGAGAATTTTATTGTTAATAAAATTAGCCGCCAACGATAGCGTTTCGTAAAAACCCACCTCTGCCCACTCGCGGCCAATAACCCCTac                                  <  1:2918723/151‑2 (MQ=255)
                                                                                                                    aaaacagaggtcggaaaatatgggggaaaatccaGGAGAAAAGCCCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCt                                    >  1:1383440‑M1/35‑148 (MQ=255)
                                                                                                                      gggTATCACCATGTGAAACGTTGTTACGCCGGGGAGTtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttagactagtcattggagaacagatgattgatgtcttagggccgg                                <  1:3588913‑M2/150‑114 (MQ=255)
                                                                                                                          aTCACCATGTGAAACGTTGTTACGCCGGGGAGTtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttagactagtcattggagaacagatgattgatgtcttagggccggagaa                            <  2:2303938‑M2/150‑118 (MQ=255)
                                                                                                                                             ttACGCCGGGGAGTtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttagactagtcattggagaacagatgattgatgtcttagggccggagaaacgcagacggcgtaccacac        <  1:488781‑M2/151‑138 (MQ=255)
                                                                                                                                              tACGCCGGGGAGTtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttagactagtcattggagaacagatgattgatgtcttagggccggagaaacgcagacggcgtaccacac        <  1:2067393‑M2/150‑138 (MQ=255)
                                                                                                                                               aaatccaGGATTAAAGCCCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCGCCCAGc        >  2:3153843‑M1/8‑149 (MQ=255)
                                                                                                                                                      ggAGTtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttagactagtcattggagaacagatgattgatgtcttagggccggagaaacgcagacggcgtaccacacaggaaa  <  2:645960‑M2/148‑144 (MQ=255)
                                                                                                                                                      |                                                                                                                                                   
ACACCGACCAGATTTTCTCTGCTCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCGCCCAGCTGAAAA  >  NC_000913/2628466‑2628763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.