New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 448189815 (0.570)3 (0.120) 3/282 2.9 17.5% coding (1940/2856 nt) valS valyl‑tRNA synthetase
?NC_000913 = 4481970 14 (0.560)coding (1868/2856 nt) valS valyl‑tRNA synthetase
Rejected: Frequency below cutoff threshold.

AGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGACGCTTCATATCCCAGTTGATGTCACGACCGGTAGACGCCAGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGG  >  NC_000913/4481749‑4482045
                                                                                                                                                     |                                                                                                                                                   
aGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGtt                                                                                                                                                    >  2:156141/1‑151 (MQ=255)
     aaGCTGTCCAGCGCTCCGCGGTCCGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCCGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCcac                                                                                                                                                <  2:366133/150‑1 (MQ=255)
                                          ggTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGACGCtt                                                                                                          >  2:38105/1‑151 (MQ=255)
                                                aCTCCGCCAGAATCCAGCGTCCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGGCCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGGTACGGTAACCTTCCAGACGCTTCATATc                                                                                                    <  2:296166/151‑1 (MQ=255)
                                                         gAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGACGCTTCATATCCCAGTTGAt                                                                                           >  1:51961/1‑151 (MQ=255)
                                                         gAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGGctaccggtcctgacatcaactgggatatgaagcgtctggaaggttaccgtaactt                                                                                              >  2:232655‑M1/1‑93 (MQ=255)
                                                           aTCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGACGCTTCATATCCCAGTTGATGt                                                                                         >  2:244967/1‑151 (MQ=255)
                                                                 cGGTCCGCCAGCGACAGCGTCATTCCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGACGCTTCATATCCCAGTTGATGTCACGAc                                                                                   <  1:123463/151‑1 (MQ=255)
                                                                            cGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTCACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTctaccggtcgtgacctcaactgcgatatgatgcgtcaggaaggttaccgtaactttcttaacaacctgttgcacgc                                                                         >  1:220242‑M1/1‑74 (MQ=255)
                                                                                      aTTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCGGGCGTTCCACAGCTTGTTACAGAAGTTACGGGAACCTTCCAGACGCTTCATATCCCAGTTGATGTCACGACCGGTAGacgccagccgctttg                                                              >  2:43783‑M2/1‑136 (MQ=255)
                                                                                                             aTCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGACGCTTCATATCCCAGTTGATGTCACGACCGGTAGACGCCAGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGTa                                       >  1:327816/1‑151 (MQ=255)
                                                                                                             aTCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGACGCTTCATATCCCAGTTGATGTCACGACCGGTAGACGCCAGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGTa                                       >  1:288605/1‑151 (MQ=255)
                                                                                                               ccTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGACGCTTCATATCCCAGTTGATGTCACGACCGGTAGACTCCAGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGTAc                                      >  2:88810/1‑150 (MQ=255)
                                                                                                                       tCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAAACTTCCAGACCCTTCATATCCCAGTTGCTGTCACGACCGGTAGACGCCAGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGTACCGTGCgg                               >  2:271072/1‑149 (MQ=255)
                                                                                                                        cTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGACGCTTCATATCCCAGTTGATGTCACGACCGGTAGACGCCAGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCt                             >  2:18682/1‑150 (MQ=255)
                                                                                                                                    gaacaAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGACGCTTCATATCCCAGTTGATGTCACGACCGGTAGACGCCAGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCgg                >  2:55614/3‑151 (MQ=255)
                                                                                                                                       caAAGCGGCTGGCGTctaccggtcgtgacatcaactgggatatgaagcgtctggaaggttcccgtaacttctgtaacaagctgtggaacgcccgccgctttgtgctgatgaacacagaaggtcaggattgcggcttcaacggcggcgaaat             >  2:43783‑M1/1‑15 (MQ=255)
                                                                                                                                                  gCGTGCAACAGGTTGTTAAGAAAGTTACGGTAACCTTCCTGACGCATCATATCGCAGTTGAGGTCACGACCGGTAGacgccagccgctttgtgctgatgaacacagaaggtgaggattgcggcttcaacggcggcgaaatgacgctgtcg   >  1:220242‑M2/1‑76 (MQ=255)
                                                                                                                                                   cGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGACGCTTCATATCCCAGTTGATGTCACGACCGGTAGACGCCAGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCgg  <  1:55608/150‑1 (MQ=255)
                                                                                                                                                   cGTTCCAAAGCTTGTTACAGAAGTTACGGTAACCTTCCAGACGCTTCATATCCCAGTTGATGTCACGACCGGTAGACGCCAGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCgg  <  2:10114/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
AGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGACGCTTCATATCCCAGTTGATGTCACGACCGGTAGACGCCAGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGG  >  NC_000913/4481749‑4482045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.