New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 448189815 (0.570)3 (0.120) 3/282 2.9 17.5% coding (1940/2856 nt) valS valyl‑tRNA synthetase
?NC_000913 = 4481970 14 (0.560)coding (1868/2856 nt) valS valyl‑tRNA synthetase
Rejected: Frequency below cutoff threshold.

ATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGACGCTTCATATCCCAGTTGATGTCACGACCGGTAGACGCCAGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCTGGCA  >  NC_000913/4481835‑4482117
                                                                                                                                       |                                                                                                                                                   
aTTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCGGGCGTTCCACAGCTTGTTACAGAAGTTACGGGAACCTTCCAGACGCTTCATATCCCAGTTGATGTCACGACCGGTAGacgccagccgctttg                                                                                                                                      >  2:43783‑M2/1‑136 (MQ=255)
                       aTCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGACGCTTCATATCCCAGTTGATGTCACGACCGGTAGACGCCAGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGTa                                                                                                               >  1:288605/1‑151 (MQ=255)
                       aTCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGACGCTTCATATCCCAGTTGATGTCACGACCGGTAGACGCCAGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGTa                                                                                                               >  1:327816/1‑151 (MQ=255)
                         ccTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGACGCTTCATATCCCAGTTGATGTCACGACCGGTAGACTCCAGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGTAc                                                                                                              >  2:88810/1‑150 (MQ=255)
                                 tCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAAACTTCCAGACCCTTCATATCCCAGTTGCTGTCACGACCGGTAGACGCCAGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGTACCGTGCgg                                                                                                       >  2:271072/1‑149 (MQ=255)
                                  cTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGACGCTTCATATCCCAGTTGATGTCACGACCGGTAGACGCCAGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCt                                                                                                     >  2:18682/1‑150 (MQ=255)
                                              gaacaAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGACGCTTCATATCCCAGTTGATGTCACGACCGGTAGACGCCAGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCgg                                                                                        >  2:55614/3‑151 (MQ=255)
                                                            gCGTGCAACAGGTTGTTAAGAAAGTTACGGTAACCTTCCTGACGCATCATATCGCAGTTGAGGTCACGACCGGTAGacgccagccgctttgtgctgatgaacacagaaggtgaggattgcggcttcaacggcggcgaaatgacgctgtcg                                                                           >  1:220242‑M2/1‑76 (MQ=255)
                                                             cGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGACGCTTCATATCCCAGTTGATGTCACGACCGGTAGACGCCAGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCgg                                                                          <  1:55608/150‑1 (MQ=255)
                                                             cGTTCCAAAGCTTGTTACAGAAGTTACGGTAACCTTCCAGACGCTTCATATCCCAGTTGATGTCACGACCGGTAGACGCCAGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCgg                                                                          <  2:10114/150‑1 (MQ=255)
                                                                      gCTTGTTACAGAAGTTACGGTAACCTTCCAGACGCTTCATATCCCAGTTGATGTCACGACCGGTAGACGCCAGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACg                                                                >  2:49952/1‑151 (MQ=255)
                                                                                 aaGTTACGGTAACCTTCCAGACGCTTCATATCCCAGTTGATGTCAGGACCGGTAGccgccagccgctttgtgctgatgaacacagaaggtcaggattgcggcttcaacggcggcgaaatgacgctgtcgctggcggaccgctggattc                                                        >  2:232655‑M2/1‑55 (MQ=255)
                                                                                                        cTTCATATCCCAGTTGATGTCACGACCGGTAGACGCCAGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCAAGCTGCGGCTGCATCATATTg                              >  1:293399/1‑151 (MQ=255)
                                                                                                         ttCATATCCCAGTTGATGTCACGACCGGTAGACGCCAGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGc                             <  1:273983/151‑1 (MQ=255)
                                                                                                                        aTGTCACGACCGGTAGACGCCAGCGCCGCCAGTGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCTGTGCGCTTACTGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTcc               <  2:363390/150‑1 (MQ=255)
                                                                                                                                    gTAGACGCCAGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTTCAGCAGTTCTGGCa  >  1:323050/1‑151 (MQ=255)
                                                                                                                                    gTAGACGCCAGCGCCGCCAGGGTAAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCTGGCa  >  1:270502/1‑151 (MQ=255)
                                                                                                                                       |                                                                                                                                                   
ATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTTCCACAGCTTGTTACAGAAGTTACGGTAACCTTCCAGACGCTTCATATCCCAGTTGATGTCACGACCGGTAGACGCCAGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCTGGCA  >  NC_000913/4481835‑4482117

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.