Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	NC_000913	3,304,813	(C)7→10	coding (1005/1245 nt)	mtr ←	tryptophan transporter of high affinity

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 3304806	0 (0.000)	18 (0.730) +CCC	12/220	0.7	100%	coding (1012/1245 nt)	mtr	tryptophan transporter of high affinity
	?	NC_000913	3304807 =	0 (0.000)					coding (1011/1245 nt)	mtr	tryptophan transporter of high affinity

ATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGCCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTACCG  >  NC_000913/3304683‑3304940                                                                                                                             |                                                                                                                                      ttcgggctgccaaagcgtttacgcgatgcacgggctaacagcgccggaacaattgccgcccagatggtagccgctaaaccagcataaccaatggcgtacaggaatccgttcgggaacaacagccCCCCCCCACAACTgg                                                                                                                         <  2:365883‑M2/15‑1 (MQ=255) ttcgggctgccaaagcgtttacgcgatgcacgggctaacagcgccggaacaattgccgcccagatggtagccgctaaaccagcataaccaatggcgtacaggaatccgttcgggaacaacagccCCCCCCCACAACTgg                                                                                                                         <  2:112807‑M2/15‑1 (MQ=255)           caaagcgtttacgcgatgcacgggctaacagcgccggaacaattgccgcccagatggtagccgctaaaccagcataaccaatggcgtacaggaatccgttcgggaacaacagccCCCCCCCACAACTGGCGGGGCAAAg                                                                                                               <  2:297621‑M2/25‑1 (MQ=255)                   tacgcgatgcacgggctaacagcgccggaacaattgccgcccagatggtagccgctaaaccagcataaccaatggcgtacaggaatccgttcgggaacaacagcccCCCCCCCACAACTGGCGGGGCAAAGGTCAGCaa                                                                                                       >  2:30988‑M2/107‑139 (MQ=255)                    tacgcgatgcacgggctaacagcgccggaacaattgccgcccagatggtagccgctaaaccagcataaccaatggcgtacaggaatccgttcgggaacaacagccCCCCCCCACAACTGGCGGGGCAAAGGTCAGCAAt                                                                                                      >  2:21411‑M2/106‑139 (MQ=255)                                            ccggaacaattgccgcccagatggtagccgctaaaccagcataaccaatggcgtacaggaatccgttcgggaacaacagccCCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGcc                                                                              >  1:162325‑M2/82‑139 (MQ=255)                                            ccggaacaattgccgcccagatggtagccgctaaaccagcataaccaatggcgtacaggaatccgttcgggaacaacagccCCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGcc                                                                              >  2:4958‑M2/82‑139 (MQ=255)                                            ccggaacaattgccgcccagatggtagccgctaaaccagcataaccaatggcgtacaggaatccgttcgggaacaacagccCCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGcc                                                                              >  2:183692‑M2/82‑139 (MQ=255)                                                   aattgccgcccagatggtagccgctaaaccagcataaccaatggcgtacaggaatccgttcgggaacaacagccCCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAgtcgt                                                                       <  1:4958‑M2/65‑1 (MQ=255)                                                             cagatggtagccgctaaaccagcataaccaatggcgtacaggaatccgttcgggaacaacagccCCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCaaa                                                             <  2:291061‑M2/75‑1 (MQ=255)                                                                tggtagccgctaaaccagcataaccaatggcgtacaggaatccgttcgggaacaacagcccCCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAg                                                          <  2:120096‑M2/78‑1 (MQ=255)                                                                atggtagccgctaaaccagcataaccaatggcgtacaggaatccgttcgggaacaacagccCCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAg                                                          <  1:183692‑M2/78‑1 (MQ=255)                                                                atggtagccgctaaaccagcacaaccaatggcgtacaggaatccgttcgggaacaacagccCCCCCCCATAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCACACAg                                                          <  1:290943‑M2/78‑1 (MQ=255)                                                                              accagcataaccaatggcgtacaggaatccgttcgggaacaacagccCCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCa                                                  <  2:354152‑M2/86‑1 (MQ=255)                                                                              accagcataaccaatggcgtacaggaatccgttcgggaacaacacccCCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCa                                                  >  1:354152‑M2/48‑133 (MQ=255)                                                                              accagcataaccaaaggcgtaacggaaaccgttcgggaacaacagccCCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGt                                            <  1:197086‑M2/92‑1 (MQ=255)                                                                                             ggcgtacaggaatccgttcgggaacaacagccCCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCg                             >  2:90743‑M2/33‑139 (MQ=255)                                                                                               cgtacaggaatccgttcgggaacaacagccCCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGtt                           >  2:165269‑M2/31‑139 (MQ=255)                                                                                                                        cagccCCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTACCg  >  2:3359‑M2/6‑139 (MQ=255)                                                                                                                             |                                                                                                                                      ATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGCCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTACCG  >  NC_000913/3304683‑3304940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.