New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 3160836 =34 (1.090)3 (0.100) 3/208 3.1 7.7% coding (311/2220 nt) ygiQ Radical SAM superfamily protein
?NC_000913 3160864 = 39 (1.300)coding (283/2220 nt) ygiQ Radical SAM superfamily protein
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CAACGCTGGGTATAAACCAGTGTGGCGCGATCCGGGCGCTTACCCGCGACGTTATCCGGCGTGTAGGCATCGTCATGACGTAAACGGCGATCGGCGGTATAACGGTTGATCATCGAATCCATGTTGCCAGCAGTAACACCGAAAAACAGATTCGGTTTACCCAGACGCATAAAGTCGTCTTTGCTGCTCCAGTCCGGCTGGGCGATGATCCCGACGCGAAAGCCCTGCGCTTCCAGCATACGACCGCAAATCGCCATCCCGAAGCTTGGG  >  NC_000913/3160704‑3160973
                                                                                                                                    |                                                                                                                                         
cAACGCTGGGTATAAACCAGTGTGGCGCGATCCGGGCGCTTACCCGCGACGTTATCCGGCGTGTAGGCATCGTCATGACGTAAACGGCGATCGGCGGTATAACGGTTGATCATCGAATCCATGTTGCCAGCAGTAacac                                                                                                                                     <  1:414799/139‑1 (MQ=255)
           ataAACCAGTGTGGCGCGTTCCGGGCGCTTACCCGCGACGTTATGCGGCGTGTAGGCATCGGCATGACGTAAACGGCGATCGGCGGGATAACGGTTGATCATCGGATCCATGTTGCCAGCAGTAACGCCGAAAAACAGa                                                                                                                          <  1:440361/139‑1 (MQ=255)
                    tgtgGCGCGATCCGGGCGCTTACCCGCGACGTTATCCGGCGTGTAGGCATCGTCATGACGTAAACGGCGATCGGCGGTATAACGGTTGATCATCGAATCCATGTTGCCAGCAGTAACACCGAAAAACAGATTCGGTTTa                                                                                                                 >  2:97353/1‑139 (MQ=255)
                         cgcgATCCGGGCGCTTACCCGCGACGTTATCCGGCGTGTAGGCATCGTCATGACGTAAACGGCGATCGGCGGTATAACGGTTGATCATCGAATCCATGTTGCCAGCAGTAACACCGAAAAACAGATTCGGTTTACCCAg                                                                                                            <  1:97353/139‑1 (MQ=255)
                                        tACCCGCGACGTTATCCGGCGTGTAGGCATCGTCATGACGTAAACGGCGATCGGCGGTATAACGGTTGATCATCGAATCCATGTTGCCAGCAGTAACACCGAAAAACAGATTCGGTTTACCCAGACGCATAAAgtcgtc                                                                                             >  1:306404/1‑139 (MQ=255)
                                        tACCCGCGACGTTATCCGGCGTGTAGGCATCGTCATGACGTAAACGGCGATCGGCGGTATAACGGTTGATCATCGAATCCATGTTGCCAGCAGTAACACCGAAAAACAGATTCGGTTTACCCAGACGCATAAAgtcgtc                                                                                             >  2:574033/1‑139 (MQ=255)
                                            cgcgACGTTATCCGGCGTGTAGGCATCGTCATGACGTAAACGGCGATCGGCGGTATAACGGTTGATCATCGAATCCATGTTGCCAGCAGTAACACCGAAAAACAGATTCGGTTTACCCAGACGCATAAAGTCGTCTTtg                                                                                         <  2:306404/139‑1 (MQ=255)
                                             gcgACGTTATCCGGCGTGTAGGCATCGTCATGACGTAAACGGCGATCGGCGGTATAACGGTTGATCATCGAATCCATGTTGCCAGCAGTAACACCGAAAAACAGATTCGGTTTACCCAGACGCATAAAGTCGTCTTtgc                                                                                        >  1:380860/1‑139 (MQ=255)
                                                               tAGGCATCGTCATGACGTAAACGGCGATCGGCGGTATAACGGTTGATCATCGAATCCATGTTGCCAGCAGTAACACCGAAAAACAGATTCGGTTTACCCAGACGCATAAAGTCGTCTTTGCTGCTCCAGTCCGGCTggg                                                                      >  2:476668/1‑139 (MQ=255)
                                                                  gCATCGTCATGACGTAAACGGCGATCGGCGGTATAACGGTTGATCATCGAATCCATGTTGCCAGCAGTAACACCGAAAAACAGATTCGGTTTACCCAGACGCATAAAGTCGTCTTTGCTGCTCCAGTCCGGCTGGGCga                                                                   <  2:380860/139‑1 (MQ=255)
                                                                      cGTCATGACGTAAACGGCGATCGGCGGTATAACGGTTGATCATCGAATCCATGTTGCCAGCAGTAACACCGAAAAACAGATTCGGTTTACCCAGACGCATAAAGTCGTCTTTGCTGCTCCAGTCCGGCTGGGCgatgat                                                               <  1:574033/139‑1 (MQ=255)
                                                                           tGACGTAAACGGCGATCGGCGGTATAACGGTTGATCATCGAATCCATGTTGCCAGCAGt                                                                                                                                          <  1:204904/59‑1 (MQ=255)
                                                                           tGACGTAAACGGCGATCGGCGGTATAACGGTTGATCATCGAATCCATGTTGCCAGCAGt                                                                                                                                          >  2:204904/1‑59 (MQ=255)
                                                                                  aaCGGCGATCGGCGGTATAACGGTTGATCATCGAATCCATGTTGCCAGCAGTAACACCGAAAAACAGATTCGGTTTACCCAGACGCATAAAGTCGTCTTtgctg                                                                                      >  2:529221/1‑104 (MQ=255)
                                                                                  aaCGGCGATCGGCGGTATAACGGTTGATCATCGAATCCATGTTGCCAGCAGTAACACCGAAAAACAGATTCGGTTTACCCAGACGCATAAAGTCGTCTTtgctg                                                                                      <  1:529221/104‑1 (MQ=255)
                                                                                  aaCGGCGATCGGCGGTATAACGGTTGATCATCGAATCCATGTTGCCAGCAGTAACACCGAAAAACAGATTCGGTTTACCCAGACGCATAAAGTCGTCTTtg                                                                                         >  1:42257/1‑101 (MQ=255)
                                                                                  aaCGGCGATCGGCGGTATAACGGTTGATCATCGAATCCATGTTGCCAGCAGTAACACCGAAAAACAGATTCGGTTTACCCAGACGCATAAAGTCGTCTTtg                                                                                         <  2:42257/101‑1 (MQ=255)
                                                                                  aaCGGCGATCGGCGGTATAACGGTTGATCATCGAATCCATGTTGCCAGCAGTAACACCGAAAAACAGATTCGGTTTACCCAGACGCATAAAGTCGTCTTTGCTGCTCCAGTCCGGCTGGGCGATGATCCCGACGCGaaa                                                   >  1:50595/1‑139 (MQ=255)
                                                                                         aTCGGCGGTATAACGGTTGATCATCGAATCCATGTTGCCAGCAGTAACACCGAAAAACAGATTCGGTTTAcc                                                                                                               <  1:70073/72‑1 (MQ=255)
                                                                                         aTCGGCGGTATAACGGTTGATCATCGAATCCATGTTGCCAGCAGTAACACCGAAAAACAGATTCGGTTTAcc                                                                                                               >  2:70073/1‑72 (MQ=255)
                                                                                          tCGGCGGTATAACGGTTGATCATCGAATCCATGTTGCCAGCAGTAACACCGAAAAACAGATTCGGTTTACCCAGACGCATAAAGTCGTCTTTGCTGCTCCAGTCCGGCTGGGCGATGATCCCGACGCGAAAGCCCTgcg                                           <  2:50595/139‑1 (MQ=255)
                                                                                                           gATCATCGAATCCATGTTGCCAGCAGTAACACCGAAAAACAGATTCGGTTTACCCAGACGCATAAAGTCGTCTTTGCTGCTCCAGTCCGGCTGGGCGATGATCCCGACGCGAAAGCCCTg                                             <  2:72015/120‑1 (MQ=255)
                                                                                                           gATCATCGAATCCATGTTGCCAGCAGTAACACCGAAAAACAGATTCGGTTTACCCAGACGCATAAAGTCGTCTTTGCTGCTCCAGTCCGGCTGGGCGATGATCCCGACGCGAAAGCCCTg                                             >  1:72015/1‑120 (MQ=255)
                                                                                                           gATCATCGAATCCATGTTGCCAGCAGTAACACCGAAAAACAGATTCGGTTTACCCAGACGCATAAAGTCGTCTTTGCTGCTCCAGTCCGGCTGGGCGATGATCCCGACGCGAAAGCCCTGCGCTTCCAGCATACGACCg                          >  1:572074/1‑139 (MQ=255)
                                                                                                           gATCATCGAATCCATGTTGCCAGCAGTAACACCGAAAAACAGATTCGGTTTACCCAGACGCATAAAGTCGTCTTTGCTGCTCCAGTCCGGCTGGGCGATGATCCCGACGCGAAAGCCCTGCGCTTCCAGCATACGACCg                          >  1:126413/1‑139 (MQ=255)
                                                                                                              catcGAATCCATGTTGCCAGCAGTAACACCGAAAAACAGATTCGGTTTACCCAGACGCATAAAGTCGTCTTtg                                                                                         >  2:222718/1‑73 (MQ=255)
                                                                                                              catcGAATCCATGTTGCCAGCAGTAACACCGAAAAACAGATTCGGTTTACCCAGACGCATAAAGTCGTCTTtg                                                                                         <  1:222718/73‑1 (MQ=255)
                                                                                                                 cGAATCCATGTTGCCAGCAGTAACACCGAAAAACAGATTCGGTTTACCCAGACGCATAAAGTCGTCTTTGCTGCTCCAGTCCGGCTGGGCGATGATCCCGACGCGAAAGCCCTGCGCTTCCAGCATACGACCGCAAATc                    >  2:256516/1‑139 (MQ=255)
                                                                                                                   aaTCCATGTTGCCAGCAGTAACACCGAAAAACAGATTCGGTTTACCCAGACGCATAAAGTCGTCTTTGCTGCTCCAGTCCGGCTGGGCGATGATCCCGACGCGAAAGCCCTGCGCTTCCAGCATACGACCGCAAATCGc                  >  2:193049/1‑139 (MQ=255)
                                                                                                                       cATGTTGCCAGCAGTAACACCGAAAAACAGATTCGGTTTACCCAGACGCATAAAGTCGTCTTTGCTGCTCCAGTCCGGCTGGGCGATGATCCCGACGCGAAAGCCCTGCGCTTCCAGCATACGACCGCAAATCGCCATc              >  1:287998/1‑139 (MQ=255)
                                                                                                                         tGTTGCCAGCAGTAACACCGAAAAACAGATTCGGTTTACCCAGACGCATAAAGTCGTCTTTGCTGCTCCAGTCCGGCTGGGCGATGATCCCGACGCGAAAGCCCTGCGCTTCCAGCATACGACCGCAAATCGCCATccc            >  1:598229/1‑139 (MQ=255)
                                                                                                                              ccAGCAGTAACACCGAAAAACAGATTCGGTTTACCCAGACGCATAAAGTCGTCTTTGCTGCTCCAGTCCGGCTGGGCGATGATCCCGACGCGAAAGCCCTGCGCTTCCAGCATACGACCGCAAATCGCCATccc            <  2:590803/134‑1 (MQ=255)
                                                                                                                              ccAGCAGTAACACCGAAAAACAGATTCGGTTTACCCAGACGCATAAAGTCGTCTTTGCTGCTCCAGTCCGGCTGGGCGATGATCCCGACGCGAAAGCCCTGCGCTTCCAGCATACGACCGCAAATCGCCATccc            >  1:590803/1‑134 (MQ=255)
                                                                                                                                   agTAACACCGAAAAACAGATTCGGTTTACCCAGACGCATAAAGTCGTCTTTGCTGCTCCAGTCCGGCTGGGCGATGATCCCGACGCGAAAGCCCTGCGCTTCCAGCATACGACCGCAAATCGCCATCCCGAAGCTTggg  <  2:126413/139‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                         
CAACGCTGGGTATAAACCAGTGTGGCGCGATCCGGGCGCTTACCCGCGACGTTATCCGGCGTGTAGGCATCGTCATGACGTAAACGGCGATCGGCGGTATAACGGTTGATCATCGAATCCATGTTGCCAGCAGTAACACCGAAAAACAGATTCGGTTTACCCAGACGCATAAAGTCGTCTTTGCTGCTCCAGTCCGGCTGGGCGATGATCCCGACGCGAAAGCCCTGCGCTTCCAGCATACGACCGCAAATCGCCATCCCGAAGCTTGGG  >  NC_000913/3160704‑3160973

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.