breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 89 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913417,5330TG57.7% ‑5.8 / 16.4 26V131G (GTG→GGG) phoBresponse regulator in two‑component regulatory system with PhoR
*NC_000913433,6340TG36.2% 52.8 / 12.3 60G60G (GGT→GGGribDfused diaminohydroxyphosphoribosylaminopyrimidine deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase
*NC_000913753,1250TA35.9% 70.4 / 21.4 39intergenic (‑330/+60)ybgD/gltAputative fimbrial‑like adhesin protein/citrate synthase
*NC_000913433,6490TG35.1% 36.1 / 10.5 57G65G (GGT→GGGribDfused diaminohydroxyphosphoribosylaminopyrimidine deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase
*NC_0009133,531,4630AC31.9% 245.3 / 22.2 119V326G (GTG→GGG) yhgEDUF4153 family putative inner membrane protein
*NC_0009132,332,8230AG31.7% 74.4 / 12.9 42pseudogene (1580/4605 nt)yfaSpseudogene, bacterial alpha2‑macroglobulin YfaS variant family; putative membrane protein
*NC_0009132,745,0890AC30.6% 131.9 / 13.2 62V84G (GTG→GGG) trmDtRNA m(1)G37 methyltransferase, SAM‑dependent
*NC_0009131,445,9810TG30.4% 202.5 / 10.9 93G34G (GGT→GGGydbLDUF1318 family protein
*NC_0009134,442,1620AT30.2% 211.3 / 38.8 97D5E (GAT→GAAmsrAmethionine sulfoxide reductase A
*NC_0009133,520,7510GT29.1% 211.1 / 34.0 110T90K (ACA→AAA) hofPDNA catabolic protein
*NC_0009131,383,7220GA28.8% 123.0 / 34.9 74F80F (TTC→TTTycjWLacI family putative transcriptional repressor
*NC_00091375,0790CT28.6% 24.5 / 12.5 21L134L (CTG→CTAthiBthiamine/thiamine pyrophosphate/thiamine monophosphate ABC transporter periplasmic binding protein
*NC_0009132,149,9070AC28.6% 139.0 / 12.0 91V360G (GTG→GGG) yegIprotein kinase‑related putative non‑specific DNA‑binding protein
*NC_0009134,296,0600CT28.4% 233.0 / 119.2 149intergenic (+266/+376)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
*NC_0009133,895,5560TC28.4% 142.3 / 11.5 67M352V (ATG→GTG) adePadenine permease, high affinity; adenine:H+ symporter
*NC_000913691,8470AG28.1% 291.0 / 22.1 131intergenic (‑63/+27)ybeX/ybeYputative ion transport/ssRNA‑specific endoribonuclease; 16S rRNA 3' end maturation and quality control co‑endoribonuclease working with RNase R; rRNA transcription antitermination factor
*NC_0009133,665,7020CA27.7% 196.5 / 31.0 83A37S (GCG→TCG) gadXacid resistance regulon transcriptional activator; autoactivator
*NC_000913647,2100AC27.6% 150.1 / 14.9 76G100G (GGT→GGGcitG2‑(5''‑triphosphoribosyl)‑3'‑dephosphocoenzyme‑A synthase
*NC_0009133,757,6680CT26.7% 122.6 / 13.9 60A54T (GCA→ACA) yiaYL‑threonine dehydrogenase
*NC_0009133,813,2720CT26.5% 79.4 / 22.3 49S181L (TCG→TTG) dfpfused 4'‑phosphopantothenoylcysteine decarboxylase/phosphopantothenoylcysteine synthetase, FMN‑binding

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =165 (1.200)29 (0.230) 27/304 2.2 18.3% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 109 (0.870)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 1207805165 (1.200)22 (0.180) 21/304 2.9 14.6% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 109 (0.870)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 1373284174 (1.260)5 (0.040) 5/320 6.5 3.1% coding (63/882 nt) ycjO putative sugar ABC transporter permease
?NC_000913 = 1373292 148 (1.130)coding (71/882 nt) ycjO putative sugar ABC transporter permease
* ? NC_000913 = 2213711120 (0.870)3 (0.020) 3/332 7.6 2.6% coding (934/1686 nt) yehU inner membrane putative sensory kinase in two‑component system with YehT
?NC_000913 2213753 = 103 (0.760)coding (892/1686 nt) yehU inner membrane putative sensory kinase in two‑component system with YehT