New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 3463082 =42 (0.670)8 (0.130) 8/290 4.6 14.1% coding (159/984 nt) gspK general secretory pathway component, cryptic
?NC_000913 3463296 = 57 (0.940)coding (373/984 nt) gspK general secretory pathway component, cryptic
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CGTTAATCAGCAGCGCACTGAATACCTGGGCGGGGAAATCAGGGGAAGCCAGCGGGTCGTCAGAGATTTTTGCTAACGCATTGAGATTAAAACAGTGCTGGGCATCGCGTAACTGCCACTTCACTGTATTGCCATCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAGTACTGCTGCAACGTGGTCTGTCTGTCGTTATCTTTAACGTCCTGGGTGAGGCTGGCAAGCGCAAGTTTCTCCGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/3463323‑3463082
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ccgccCAGGTATTCAGTGCGCTGCTGATTAACGCCGGTATCGATCGCGGAAACACCGATGAGATCGTGCAATCAATCGCTGACTATATTGATGTCGATGACTCACCACGCTTTCACGGCGCAGAAGATAGCTTTTACCAAAGCCAGACGCCGCCCCGGCATAGTGCCA  >  NC_000913/3463296‑3463458
                                                                                                                                                                                                                                                                                                                                                                                                                     
CGTTAATCAGCAGCGCACTGAATACCTGGGCGGGGAAATCAGGGGAAGCCAGCGGGTCGTCAGAGATTTTTGCTAACGCATTGAGATTAAAACAGTGCTGGGCATCGCGTAACTGCCACTTCACTGTATTGCCATATTCCAGCTGCAACTGTTGTGGCTGTGCCCAGTACTGCTGCAACGTGGTCTGTCTGTCGTTATCTTTAACGTCCTGGGTGAGGCTGGCAAGCGCAAGTTTCTCC                                                                                                                                                                        >  1:885245/1‑239
   gAATCAGCAGCGCACTGAATCCCTGGGCGGGGAAATCAGGGGAAGCCAGCGGGTCGTCAGAGATTTTTGCTAACGCATTGAGATTAAAACAGTGCTGGGCATCGCGTAACTGCCACTTCACTGTATTGCCATCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAGTACTGCTGCAACGTGGTCTGTCTGTCGTTATCTTTAACGTCCTGGGTGAGGCTGGCAAGCGCAAGTTTCTCCGC                                                                                                                                                                      <  1:561682/237‑1
                                                                                               TGCTGGGCATCGCGTAACTGCCACTTCACTGTATTGCCATCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAGTACTGCTGCAACGTGGTCTGTCTGTCGTTATCTTTAACGTCCTGGGTGAGGCTGGCAAGCGCAAGTTTCTCCGC                                                                                                                                                                      >  1:406526/1‑146
                                                                                               TGCTGGGCATCGCGTAACTGCCACTTCACTGTATTGCCATCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAGTACTGCTGCAACGTGGTCTGTCTGTCGTTATCTTTAACGTCCTGGGTGAGGCTGGCAAGCGCAAGTTTCTCCGC                                                                                                                                                                      <  2:406526/146‑1
                                                                                                                                                                       TACTGCTGCAACGTGGTCTGTCTGTCGTTATCTTTAACGTCCTGGGTGAGGCTGGCAAGCGCAAGTTTCTCCGCCCAGGTATTCAGTGCGCTGCTGATTAACGCCGGTATCGATCGCGGAAACACCGATGAGATCGTGCAATCAATCGCTGACTATATTGATGTCGATGACTCACCACGCTTTCACGGCGCAGAAGATAGCTTTTACCAAAGCCAGACGCCGCCCCGGCATAGTGCCA  >  1:959126/1‑238
                                                                                                                                                                        tCTGCTGCAACGTGGTCTGTCTGTCGTTATCTTTAGCGTCCTGGGTGAGGCTGGCAAGCGCAAGTTTCGCCGCCCAGGTATTCAGTGCGCTGCTGATTAACGCCGGTATCGATCGCGGAAACACCGATGAGATCGTGCAATCAATCGCTGACTATATTGATGTCGATGACTCACCACGCTTTCACGGCGCAGAAGATAGCTTTTACCAAAGCCAGACGCCGCCCCGGCATAGTGC    <  2:959126/234‑1
                                                                                                                                                                                      GTCTGTCTGTCGTTATCTTTAACGTCCTGGGTGAGGCTGGCAAGCGCAAGTTTCTCCGCCCAGGTATTCAGTGCGCTGCTGATTAACGCCGGTATCGATCGCGGAAACACCGATGAGATCGTGCAATCAATCGCTGACTATATTGATGTCGATGACTCACCACGCTTTCACGGCGCAGAAGATAGCTTTTACCAAAGCCAGACGCCGCCCC              <  1:216807/211‑1
                                                                                                                                                                                      GTCTGTCTGTCGTTATCTTTAACGTCCTGGGTGAGGCTGGCAAGCGCAAGTTTCTCCGCCCAGGTATTCAGTGCGCTGCTGATTAACGCCGGTATCGATCGCGGAAACACCGATGAGATCGTGCAATCAATCGCTGACTATATTGATGTCGATGACTCACCACGCTTTCACGGCGCAGAAGATAGCTTTTACCAAAGCCAGACGCCGCCCC              >  2:216807/1‑211
                                                                                                                                                                                                                         GCTGGCAAGCGCAAGTTTCTCCGCCCAGGTATTCAGTGCGCTGCTGATTAACGCCGGTATCGATCGCGGAAACACCGATGAGATCG                                                                                                        <  1:811408/86‑1
                                                                                                                                                                                                                         GCTGGCAAGCGCAAGTTTCTCCGCCCAGGTATTCAGTGCGCTGCTGATTAACGCCGGTATCGATCGCGGAAACACCGATGAGATCG                                                                                                        >  2:811408/1‑86
                                                                                                                                                                                                                              CAAGCGCAAGTTTCTCCGCCCAGGTATTCAGCGCGCTGCTGATTAACGCCGGTATCGATCGCGGAAACACCGATGAGATCGTGCAATCAATCGCTGACTATATTGATGTCGATGACTCACCACGCTTTCACGGCGCAGAAGATAGCTTTTACCAAAGCCA                         <  1:708849/160‑1
                                                                                                                                                                                                                              CAAGCGCAAGTTTCTCCGCCCAGGTATTCAGCGCGCTGCTGATTAACGCCGGTATCGATCGCGGAAACACCGATGAGATCGTGCAATCAATCGCTGACTATATTGATGTCGATGACTCACCACGCTTTCACGGCGCAGAAGATAGCTTTTACCAAAGCCA                         >  2:708849/1‑160
                                                                                                                                                                                                                                                                                                                                                                                                                     
CGTTAATCAGCAGCGCACTGAATACCTGGGCGGGGAAATCAGGGGAAGCCAGCGGGTCGTCAGAGATTTTTGCTAACGCATTGAGATTAAAACAGTGCTGGGCATCGCGTAACTGCCACTTCACTGTATTGCCATCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAGTACTGCTGCAACGTGGTCTGTCTGTCGTTATCTTTAACGTCCTGGGTGAGGCTGGCAAGCGCAAGTTTCTCCGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/3463323‑3463082
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ccgccCAGGTATTCAGTGCGCTGCTGATTAACGCCGGTATCGATCGCGGAAACACCGATGAGATCGTGCAATCAATCGCTGACTATATTGATGTCGATGACTCACCACGCTTTCACGGCGCAGAAGATAGCTTTTACCAAAGCCAGACGCCGCCCCGGCATAGTGCCA  >  NC_000913/3463296‑3463458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.