Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	3,966,174	IS1 (–) +9 bp	intergenic (+84/‑235)	trxA → / → rho	thioredoxin 1/transcription termination factor

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	3583428 =	NA (NA)	39 (0.890)	30/298	0.2	100%	noncoding (1/768 nt)	IS1	repeat region
	?	NC_000913	3966174 =	0 (0.000)					intergenic (+84/‑243)	trxA/rho	thioredoxin 1/transcription termination factor
*	?	NC_000913	= 3584195	NA (NA)	47 (1.080)	27/298	0.3	100%	noncoding (768/768 nt)	IS1	repeat region
	?	NC_000913	= 3966182	0 (0.000)					intergenic (+92/‑235)	trxA/rho	thioredoxin 1/transcription termination factor

GAGATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/3583575‑3583428 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACAGATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACTCTCCTGTGACCAGGCAGCGAAAAGACATG  >  NC_000913/3966174‑3966323                                                                                                                                                                                                                                                                                                            GAGATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCAA                                                                                                                                                      >  1:300438/1‑150  AGATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCAACA                                                                                                                                                    >  2:206451/1‑151  AGATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCAACA                                                                                                                                                    >  1:592453/1‑151  AGATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCAACA                                                                                                                                                    >  1:514264/1‑151    ATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCAACAT                                                                                                                                                   <  2:609036/150‑1        CGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCAACATACC                                                                                                                                                <  1:621650/149‑1            GATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCAACATACCTTATTA                                                                                                                                          >  2:255313/1‑151              TGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCAACATACCTTATTAAG                                                                                                                                        <  2:207102/151‑1                     CGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCAACATACCTTATTAAGTTTGAAT                                                                                                                                 <  1:102625/151‑1                       GTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCAACATACCTTATTAAGTTTGAATC                                                                                                                                <  1:88043/150‑1                       GTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCAACATACCTTATTAAGTTTGAATC                                                                                                                                <  1:230101/150‑1                                  GTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCAACATACCTTATTAAGTTTGAATCTTGTAATTTCCA                                                                                                                    >  2:418071/1‑151                                      CGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGC                                                                                                                <  1:66930/151‑1                                      CGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGC                                                                                                                <  1:627797/151‑1                                            ACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCG                                                                                                          <  1:581980/151‑1                                             CCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGT                                                                                                         >  2:244076/1‑151                                                                GGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGT                                                                                      >  2:461408/1‑151                                                                     GACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAAC                                                                                  <  1:229972/150‑1                                                                     GACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAAC                                                                                  <  2:100390/150‑1                                                                            GATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACAGATTT                                                                            >  1:16182/1‑149                                                                                 AAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCAACATACCTTATTAAGTTAGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACAGATTTCTGGCT                                                                      <  2:245501/150‑1                                                                                   ACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACAGATTTCTGGCTCGT                                                                   >  2:322569/1‑151                                                                                          CCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACAGATTTCTGGCTCGTCACTCAA                                                            >  2:96667/1‑151                                                                                          CCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACAGATTTCTGGCTCGTCACTCAA                                                            >  1:109887/1‑151                                                                                          CCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACAGATTTCTGGCTCGTCACTC                                                              <  1:673620/149‑1                                                                                            ACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACAGATTTCTGGCTCGTCACTCAATC                                                          <  1:164827/151‑1                                                                                                     CCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACAGATTTCTGGCTCGTCACTCAATCCGTCTTGT                                                  >  2:18143/1‑150                                                                                                     CCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACAGATTTCTGGCTCGTCACTCAATCCGTCTTGT                                                  >  2:638152/1‑150                                                                                                      CTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACAGATTTCTGGCTCGTCACTCAATCCGTCTTGTCG                                                <  2:654637/151‑1                                                                                                        CAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACAGATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTT                                              >  2:598123/1‑151                                                                                                               ACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACAGATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTC                                       <  1:356704/151‑1                                                                                                                       ACACTAAATCAGTAAGTTGGCAGCATCACCAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACAGATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACT                               <  2:33195/151‑1                                                                                                                           TAAATCAGTAAGTTGGCAGCATCACCAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACAGATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACTCTCC                           <  1:251623/151‑1                                                                                                                              ATCAGTAAGTTGGCAGCATCACCAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACAGATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACTCTCCTGT                        <  2:204582/151‑1                                                                                                                                   TAAGTTGGCAGCATCACCAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACAGATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACTCTCCTGTGAC                     <  1:129520/149‑1                                                                                                                                       TTGGCAGCATCACCAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACAGATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACTCTCCTGTGACCAGGC                <  1:578663/150‑1                                                                                                                                       TTGGCAGCATCACCAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACAGATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACTCTCCTGTGACCAGGC                <  1:472176/150‑1                                                                                                                                       TTGGCAGCATCACCAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACAGATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACTCTCCTGTGACCAGGC                <  2:672761/150‑1                                                                                                                                                    CAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACAGATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACTCTCCTGTGACCAGGCAGCGAAAAGACATG  <  1:56895/151‑1                                                                                                                                                                                                                                                                                                            GAGATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/3583575‑3583428 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACAGATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACTCTCCTGTGACCAGGCAGCGAAAAGACATG  >  NC_000913/3966174‑3966323

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.