breseq  version 0.27.1  revision 87c22d663cc3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 1,403 A→G Y356C (TAC→TGC)  thrA → Bifunctional aspartokinase/homoserine dehydrogenase 1
MC JC 257,908 Δ776 bp [crl] [crl]
RA 450,073 C→T W190* (TGG→TAG)  cyoB ← cytochrome o ubiquinol oxidase subunit I
RA 822,421 C→T L26L (TTG→TTA ybhN ← UPF0104 family inner membrane protein
RA 933,022 A→G D143G (GAC→GGC)  lrp → leucine‑responsive global transcriptional regulator
MC JC 1,299,499 Δ1,199 bp intergenic (+254/‑485) ychE → / → oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
RA 1,818,103 A→T V133E (GTA→GAA)  chbF ← phospho‑chitobiase; general 6‑phospho‑beta‑glucosidase activity
MC JC 1,978,503 Δ776 bp insB1insA insB1, insA
JC JC 1,979,486 IS5 (+) +4 bp intergenic (‑271/‑264) insA ← / → uspC IS1 repressor TnpA/universal stress protein
RA 2,173,363 Δ2 bp intergenic (‑1/+1) gatC ← / ← gatC pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
JC JC 2,492,323 IS1 (–) +9 bp coding (924‑932/1251 nt) frc ← formyl‑CoA transferase, NAD(P)‑binding
RA 2,907,364 A→T L193Q (CTG→CAG)  eno ← enolase
RA 2,907,452 C→G V164L (GTT→CTT)  eno ← enolase
RA 3,060,146 C→A Q132K (CAG→AAG)  argP → transcriptional regulator for arginine transport and DNA replication genes; replication initiation inhibitor
RA 3,471,274 C→A G19V (GGC→GTC)  tufA ← translation elongation factor EF‑Tu 1
RA 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
JC JC 3,966,174 IS1 (–) +9 bp intergenic (+84/‑235) trxA → / → rho thioredoxin 1/transcription termination factor
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,430,521 A→G I220V (ATA→GTA)  cycA → D‑alanine/D‑serine/glycine transporter

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1896928 1898286 1359 6 [4] [4] 5 [sdaA] [sdaA]
* * ÷ NC_000913 2684277 2685525 1249 8 [0] [4] 5 [glyA] [glyA]
* * ÷ NC_000913 2929579 2930928 1350 8 [0] [0] 9 sdaB L‑serine dehydratase 2
* * ÷ NC_000913 3258300 3259646 1347 10 [0] [0] 9 tdcG L‑serine dehydratase 3, anaerobic
* * ÷ NC_000913 3423790–3424234 3424506–3424238 5–717 5 [4] [3] 5 [rrlD] [rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 381689NA (NA)7 (0.210) 5/278 2.4 NA noncoding (430/1331 nt) IS2 repeat region
?NC_000913 = 381743 NA (NA)noncoding (484/1331 nt) IS2 repeat region
* ? NC_000913 1615598 =37 (1.070)1314 (39.440) 149/284 0.0 97.2% coding (795/882 nt) yneJ putative DNA‑binding transcriptional regulator
?NC_000913 = 1625301 40 (1.200)intergenic (+10/+34) ydeJ/dcp inactive PncC family protein/dipeptidyl carboxypeptidase II
* ? NC_000913 3619878 =NA (NA)6 (0.180) 5/278 2.4 100% coding (687/4236 nt) rhsB Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
?NC_000913 3619892 = 0 (0.000)coding (701/4236 nt) rhsB Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
* ? NC_000913 4498181 =NA (NA)24 (0.690) 8/296 1.8 NA noncoding (1/1331 nt) IS2 repeat region
?NC_000913 = 4499512 NA (NA)intergenic (+12/+81) insD1/yjgX IS2 transposase TnpB/pseudogene fragment
* ? NC_000913 = 454269029 (0.840)7 (0.210) 5/278 2.4 23.2% intergenic (+57/‑425) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
?NC_000913 = 4542986 19 (0.580)intergenic (+353/‑129) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)