breseq version 0.27.1 revision 87c22d663cc3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||
---|---|---|---|---|---|
evidence | position | mutation | annotation | gene | description |
RA | 1,403 | A→G | Y356C (TAC→TGC) | thrA → | Bifunctional aspartokinase/homoserine dehydrogenase 1 |
MC JC | 257,908 | Δ776 bp | [crl] | [crl] | |
RA | 450,073 | C→T | W190* (TGG→TAG) | cyoB ← | cytochrome o ubiquinol oxidase subunit I |
RA | 822,421 | C→T | L26L (TTG→TTA) | ybhN ← | UPF0104 family inner membrane protein |
RA | 933,022 | A→G | D143G (GAC→GGC) | lrp → | leucine‑responsive global transcriptional regulator |
MC JC | 1,299,499 | Δ1,199 bp | intergenic (+254/‑485) | ychE → / → oppA | UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein |
RA | 1,818,103 | A→T | V133E (GTA→GAA) | chbF ← | phospho‑chitobiase; general 6‑phospho‑beta‑glucosidase activity |
MC JC | 1,978,503 | Δ776 bp | insB1–insA | insB1, insA | |
JC JC | 1,979,486 | IS5 (+) +4 bp | intergenic (‑271/‑264) | insA ← / → uspC | IS1 repressor TnpA/universal stress protein |
RA | 2,173,363 | Δ2 bp | intergenic (‑1/+1) | gatC ← / ← gatC | pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC |
JC JC | 2,492,323 | IS1 (–) +9 bp | coding (924‑932/1251 nt) | frc ← | formyl‑CoA transferase, NAD(P)‑binding |
RA | 2,907,364 | A→T | L193Q (CTG→CAG) | eno ← | enolase |
RA | 2,907,452 | C→G | V164L (GTT→CTT) | eno ← | enolase |
RA | 3,060,146 | C→A | Q132K (CAG→AAG) | argP → | transcriptional regulator for arginine transport and DNA replication genes; replication initiation inhibitor |
RA | 3,471,274 | C→A | G19V (GGC→GTC) | tufA ← | translation elongation factor EF‑Tu 1 |
RA | 3,560,455 | +G | intergenic (‑2/+1) | glpR ← / ← glpR | pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon |
JC JC | 3,966,174 | IS1 (–) +9 bp | intergenic (+84/‑235) | trxA → / → rho | thioredoxin 1/transcription termination factor |
RA | 4,296,381 | +GC | intergenic (+587/+55) | gltP → / ← yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
RA | 4,430,521 | A→G | I220V (ATA→GTA) | cycA → | D‑alanine/D‑serine/glycine transporter |
Unassigned missing coverage evidence | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | NC_000913 | 1896928 | 1898286 | 1359 | 6 [4] | [4] 5 | [sdaA] | [sdaA] |
* | * | ÷ | NC_000913 | 2684277 | 2685525 | 1249 | 8 [0] | [4] 5 | [glyA] | [glyA] |
* | * | ÷ | NC_000913 | 2929579 | 2930928 | 1350 | 8 [0] | [0] 9 | sdaB | L‑serine dehydratase 2 |
* | * | ÷ | NC_000913 | 3258300 | 3259646 | 1347 | 10 [0] | [0] 9 | tdcG | L‑serine dehydratase 3, anaerobic |
* | * | ÷ | NC_000913 | 3423790–3424234 | 3424506–3424238 | 5–717 | 5 [4] | [3] 5 | [rrlD] | [rrlD] |
Unassigned new junction evidence | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913 | = 381689 | NA (NA) | 7 (0.210) | 5/278 | 2.4 | NA | noncoding (430/1331 nt) | IS2 | repeat region |
? | NC_000913 | = 381743 | NA (NA) | noncoding (484/1331 nt) | IS2 | repeat region | |||||
* | ? | NC_000913 | 1615598 = | 37 (1.070) | 1314 (39.440) | 149/284 | 0.0 | 97.2% | coding (795/882 nt) | yneJ | putative DNA‑binding transcriptional regulator |
? | NC_000913 | = 1625301 | 40 (1.200) | intergenic (+10/+34) | ydeJ/dcp | inactive PncC family protein/dipeptidyl carboxypeptidase II | |||||
* | ? | NC_000913 | 3619878 = | NA (NA) | 6 (0.180) | 5/278 | 2.4 | 100% | coding (687/4236 nt) | rhsB | Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor |
? | NC_000913 | 3619892 = | 0 (0.000) | coding (701/4236 nt) | rhsB | Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor | |||||
* | ? | NC_000913 | 4498181 = | NA (NA) | 24 (0.690) | 8/296 | 1.8 | NA | noncoding (1/1331 nt) | IS2 | repeat region |
? | NC_000913 | = 4499512 | NA (NA) | intergenic (+12/+81) | insD1/yjgX | IS2 transposase TnpB/pseudogene fragment | |||||
* | ? | NC_000913 | = 4542690 | 29 (0.840) | 7 (0.210) | 5/278 | 2.4 | 23.2% | intergenic (+57/‑425) | fimE/fimA | tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin) |
? | NC_000913 | = 4542986 | 19 (0.580) | intergenic (+353/‑129) | fimE/fimA | tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin) |