New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? USA300TCH1516_ALE = 91289221 (0.920)3 (0.140) 3/268 2.7 12.4% coding (317/1308 nt) sufB_1 FeS cluster assembly protein SufB
?USA300TCH1516_ALE = 912909 22 (1.000)coding (334/1308 nt) sufB_1 FeS cluster assembly protein SufB
Rejected: Frequency below/above cutoff threshold.

CAACACGATGTAAAAGGTGATGTTTATCAATCTTTATCACAATTACCTGAGTCAGTAAGAGAAATTATTGACGTAGATCATTCTAAAAACTTAGTAATTCAACATAATAATACGATTGCGTACACACAAGTTGATGATAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  USA300TCH1516_ALE/912753‑912892
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gataaCGCCATCTTTCGAT‑GCATTATCATCAACTTGTGTGTACGCAATCGTATTATTATGTTGAATTACTAAGTTTTTAGAATGATCTACGTCAATAATTTCTCTTACTGACTCAGGTAATTGTGATAAAGATTGATAAACA  <  USA300TCH1516_ALE/912909‑912773
                                                                                                                                                                                                                                                                                      
CAACACGATGTAAAAGGTGATGTTTATCAATCTTTATCACAATTACCTGAGTCAGTAAGAGAAATTATTGACGTAGATCATTCTAAACACTTAGTAATTCAACATAATAATACGATTGCGTACACACAAGTTGATGATAA                                                                                                                                            >  1:83527/1‑140
                                    TCACAATTACCTGAGTCAGTAAGAGAAATTATTGACGTAGATAATTCTATATACTTAGTAATTCAACATAATAATACGATTGCGTACACACAAGTTGATGATAACGCCATCTTTCGAT‑GCATTATCATCAACTTGTGTGT                                                                                                       <  1:86552/140‑1
                                               TGAGTCAGTAAGAGAAATTATTGACGTAGATCATTCTAAAAACTTAGTAATTCAACATAATAATACGATTGCGTACACACAAGCAGATGATAGC‑TCATCGGAAGATGGCGTTATCATCAACTTGTGTGTACGCAATCGTA                                                                                            >  1:86653/1‑140
                                                                                                              TACGATTGCGTACACACAAGTTGATGATAACG‑CATCGAAAGATGGCGTTATCATCAACTTGTGTGTACGCAATCGTATTATTATGTTGAATTACTAAGTTTTTAGAATGATCTACGTCAATAATTTCTCTTACTGACTCA                             <  2:86653/140‑1
                                                                                                                                         TAACGCCATCTTTCGAT‑GCATTATCATCAACTTGTGTGTACGCAATCGTATTATTATGTTGAATTACTAAGTTTTTAGAATGATCTACGTCAATAATTTCTCTTACTGACTCAGGTAATTGTGCTCAAGCTAGAGAAACA  >  1:60506/1‑140
                                                                                                                                                                                                                                                                                      
CAACACGATGTAAAAGGTGATGTTTATCAATCTTTATCACAATTACCTGAGTCAGTAAGAGAAATTATTGACGTAGATCATTCTAAAAACTTAGTAATTCAACATAATAATACGATTGCGTACACACAAGTTGATGATAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  USA300TCH1516_ALE/912753‑912892
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gataaCGCCATCTTTCGAT‑GCATTATCATCAACTTGTGTGTACGCAATCGTATTATTATGTTGAATTACTAAGTTTTTAGAATGATCTACGTCAATAATTTCTCTTACTGACTCAGGTAATTGTGATAAAGATTGATAAACA  <  USA300TCH1516_ALE/912909‑912773

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.