| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | USA300TCH1516_ALE | = 912892 | 21 (0.920) | 3 (0.140) | 3/268 | 2.7 | 12.4% | coding (317/1308 nt) | sufB_1 | FeS cluster assembly protein SufB |
| ? | USA300TCH1516_ALE | = 912909 | 22 (1.000) | coding (334/1308 nt) | sufB_1 | FeS cluster assembly protein SufB | |||||
| Rejected: Frequency below/above cutoff threshold. | |||||||||||
CAACACGATGTAAAAGGTGATGTTTATCAATCTTTATCACAATTACCTGAGTCAGTAAGAGAAATTATTGACGTAGATCATTCTAAAAACTTAGTAATTCAACATAATAATACGATTGCGTACACACAAGTTGATGATAATGC‑ATCGAAAGATGGCGTTATCGTTGAAGGTTTAGCAGACGCTCTTATGAACCATAGTGATTTAGTACAAAAGTACTTTATGAAAGATGCAGTAACAGTAGATGAACATCGTATCACAGCGCTACACACGGCATTAG > USA300TCH1516_ALE/912753‑913029 | caacaCGATGTAAAAGGTGATGTTTATCAATCTTTATCACAATTACCTGAGTCAGTAAGAGAAATTATTGACGTAGATCATTCTAAACACTTAGTAATTCAACATAATAATACGATTGCGTACACACAAGTTGATGATAA > 1:83527‑M1/1‑140 (MQ=255) acacGATGTAAAAGGTGATGTTTATCAATCTTTATCACAATTACCTGAGTCAGTAAGAGAAATTATTGACGTAGATCATTCTAAAAACTTAGTAATTCAACATAATAATACGATTGCGTACACACAAGTTGATGATAATg > 2:145747/1‑140 (MQ=255) tGTTTCTCTAGCTTGAGCACAATTACCTGAGTCAGTAAGAGAAATTATTGACGTAGATCATTCTAAAAACTTAGTAATTCAACATAATAATACGATTGCGTACACACAAGTTGATGATAATGC‑ATCGAAAGATGGCGtta > 1:60506‑M2/1‑137 (MQ=255) aaTCTTTATCACAATTACCTGAGTCAGTAAGAGAAATTATTGACGTAGATCATTCTAAAAACTTAGTAATTCAACATAATAATACGATTGCGTAAACACAAGTTGATGATAATGC‑ATCGAAAGATGGCGTTATCGTTGaa > 1:644/1‑140 (MQ=255) tCTGTAACACAATTACCTGAGGCAGTAAGAGAAATTATTGACGTAGATCATTCTAAAAACTTAGTAATTCAACATAATAATACGATTGCGTACACACAAGTTGATGATAATGC‑ATCGAAAGATGGCGTTATCGTTGAAgg < 1:109018/140‑1 (MQ=255) ttATCACAATTACCTGAGTCAGTAAGAGAAATTATTGACGTAGATCATTCTAAAAACTTAGTAATTCAACATAATAATACGATTGCGTACACACAAGTTGATGATAATGC‑ATCGAAAGATGGCGTTATCGTTGAAGGttt < 1:161103/140‑1 (MQ=255) attaaaaTTACCTGAGTCAGTAAGAGAAATTATTGATGTAGATTATTCTAAAAACTTAGTAATTCAACATAATAATACGATTGCGTACACACAAGTTGATGATAATGC‑ATCGAAAGATGGCGTTATCGTTGAAGGTTTAg < 2:224763/135‑1 (MQ=255) tCACAATTACCTGAGTCAGTAAGAGAAATTATTGACGTAGATAATTCTATATACTTAGTAATTCAACATAATAATACGATTGCGTACACACAAGTTGATGATAAcgccatctttcgatgcattatcatcaacttgtgtgt < 1:86552‑M1/140‑37 (MQ=255) tGAGTCAGTAAGAGAAATTATTGACGTAGATCATTCTAAAAACTTAGTAATTCAACATAATAATACGATTGCGTACACACAAGTTGATGATAACGCCATCTTTCGATG‑CGttatcatcaacttgtgtgtacgcaatcgta < 2:86653‑M2/140‑31 (MQ=255) tGAGTCAGTAAGAGAAATTATTGACGTAGATCATTCTAAAAACTTAGTAATTCAACATAATAATACGATTGCGTACACACAAGCAGATGATAGctcatcggaagatggcgttatcatcaacttgtgtgtacgcaatcgta > 1:86653‑M1/1‑93 (MQ=255) aGTCAGTAAGAGAAATTATTGACGTAGATCATTCTAAAAACTTAGTAATTCAACATAATAATACGATTGCGTACACACAAGTTGATGATAATGC‑ATCGAAAGATGGCGTTATCGTTGAAGGTTTAGCAGACGCTCTTATg < 1:82324/140‑1 (MQ=255) attaatGACGTAGATCATTCTAAAAACTTAGTAATTCAACATAATAATACGATTGCGTACACACAAGTTGATGATAATGC‑GTCGAAAGATGGCGTTATCGTTGAAGGTTTAGCAGACGCTCTTATGAACCATAGTGAttt < 2:195110/140‑1 (MQ=255) ttGACGTAGATCATTCTAAAAACTTAGTAATTCAACATAATAATACGATTTCGTACACACAAGTTGATGATAATGC‑ATCGAAAGATAGCGTTGTCGTTGAAGGTTTAGCAGACGCTCTTATGAACCATAGTGATTTAGTa > 2:23319/1‑140 (MQ=255) ttAGTAATTCAACATAATAATACGATTGCGTACACACAAGTTGATGATAATAC‑ATAGAAAGATGGCGTTATCGTTGAAGGTTTAGCCCACGCACGTATGAACAATAGTCATTTAGTACAAAAGTACTTTATGAAAGAttc > 2:159885/1‑138 (MQ=255) aGTAATTCAACATAATAATACGATTGCGTACACACAAGTTGATGATAATGC‑ATCGAAAGCTGGCGGTATCGTTGAAGGTTTAGCAGACACTCTTATGAACCATAGTGATTTAGTACAAAAGTACTTTATGAAAGATGCAg > 2:16239/1‑140 (MQ=255) aGTAATACAACATAATAATACGATTGCGTACACACAAGTTGATGATAATGC‑ATCGAAAGATGGCGTTATCGTTGAAGGTTTAGCAGACGCTCTTATGAACCATAGTGATTTAGTACAAAAGTACTTTATGAAAGATGCAg < 1:85575/140‑1 (MQ=255) cATAATAATACGATTGCGTACACACAAGTTGATGATAATGC‑ATCGAAAGATGGCGTTATCGTTGAAGGTTTAGCAGACGCTCTTATGACCCATAGTGATTTAGTACACAAGTACTTTCTGAAAGATGCAGTCACAGTAGa > 1:234367/1‑140 (MQ=255) cATAATAATACGATTGCGTACACACAAGTTGATGATAATGC‑ATCGAAAGATGGCGTTATCGTTGAAGGTTTAGCAGACGCTCTTATGAACCATAGAGATTTAGTACAAAAGTACTTTATGAACGATGCAGTAACAGTAGa > 2:207637/1‑140 (MQ=255) ataataCGATTGCGTACACACAAGTTGATGATAATGC‑ATCGAAAGATGGCGTTATCGTTGAAGGTTTAGCAGACGCTCTTATGAACCATAGTGATTTAGTACAAAAGTACTTTATGAAAGATGCAGTAACAGTAGATGaa > 2:254233/1‑140 (MQ=255) ataataCGATTGCGTACACACAAGTTGATGATAATGC‑ATCGAAAGATGGCGTTATCGTTGAAGGTTTAGCAGACGCTCTTATGAACCATAGTGATTTAGTACAAAAGAAATAAATGGAAGATGCAGTAACAGTAGATGaa > 2:1424/1‑140 (MQ=255) taCGATTGCGTACACACAAGTTGATGATAAcgcatcgaaagatggcgttatcatcaacttgtgtgtacgcaatcgtattattatgttgaattactaagtttttagaatgatctacgtcaataatttctcttactgactca < 2:86653‑M1/140‑111 (MQ=255) taCGATTGCGTACACACAAGTTGATGATAACGCCATCTTCCGATGA‑Gctatcatctgcttgtgtgtacgcaatcgtattattatgttgaattactaagtttttagaatgatctacgtcaataatttctcttactgactca > 1:86653‑M2/1‑47 (MQ=255) cGTACACACAAGTTGATGATAATGC‑ATCGAAAGATGGCGTTATCGTTGAAGGTTTAGCAGACGCTCTTATGAACCATAGTGATTTAGTACAAAAGTACTTTATGAAAGATGCAGTAACAGTAGATGAACATCGTATcaca > 2:32855/1‑140 (MQ=255) acacacAAGTTGATGATAATGC‑ATCGAAAGATGGCGttatcatcaacttgtgtgtacgcaatcgtattattatgttgaattactaagtatatagaattatctacgtcaataatttctcttactgactcaggtaattgtga < 1:86552‑M2/140‑105 (MQ=255) gcaAGTTGATGAGAATGC‑ATCGAAAGATGGCGTTATCGTTGAAGGTTTAGCAGACGCTCTTATGAACCATAGTGATTTAGTACAAAAGTACTTTATGAAATATGCAGTAACAGTAGATGAACATCGTATCACAGCGCTac < 2:140516/139‑1 (MQ=255) gTTGATGATAATTC‑ATTGTTAGATGGTGTTATCGTTGAAGGTTTAGCAGACGCTCGTATGAACCATAGTGATTTAGTACAAAAGTACTTTATGAAAGATGCAGTAACAGTAGATGAACATCGTATCACAGCGCTacacac < 1:206052/140‑1 (MQ=255) gatgatAATGC‑ATCGAAAGATGGCGTTATCGTTGAAGGTTTAGCAGACGCTCTTATGAACCATAGTGATTTAGTACAAAAGTACTTTATGAAAGATGCAGTAACAGTAGATGAACATCGTATCACAGCGCTACACACGGc > 1:124606/1‑140 (MQ=255) atAATGC‑ATCGAAAGATGGCGTTATCGTTGAAGGTTTAGCAGACGCTCTTATGAACCATAGTGATTTAGTACAAAAGTACTTTATGAAAGATGCAGTAACAGTAGATGAACATCGTATCACAGCGCTACACACGGCATTa > 1:27575/1‑140 (MQ=255) tAAcgccatctttcgatgcattatcatcaacttgtgtgtacgcaatcgtattattatgttgaattactaagtttttagaatgatctacgtcaataatttctcttactgactcaggtaattgtgctcaagctagagaaaca > 1:60506‑M1/1‑3 (MQ=255) tAATGC‑ATCGAAAGATGGCGTTATCGTTGTTGTTTTTGCAGACACTCTTATGAACCATAGTGATTTAGTACAAAAGTACTTTATGAAAGATGCAGTAACAGTAGATGAACATCGTATCACAGCGCTACACACGGCATTAg < 1:8620/140‑1 (MQ=255) | CAACACGATGTAAAAGGTGATGTTTATCAATCTTTATCACAATTACCTGAGTCAGTAAGAGAAATTATTGACGTAGATCATTCTAAAAACTTAGTAATTCAACATAATAATACGATTGCGTACACACAAGTTGATGATAATGC‑ATCGAAAGATGGCGTTATCGTTGAAGGTTTAGCAGACGCTCTTATGAACCATAGTGATTTAGTACAAAAGTACTTTATGAAAGATGCAGTAACAGTAGATGAACATCGTATCACAGCGCTACACACGGCATTAG > USA300TCH1516_ALE/912753‑913029 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |