New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	USA300TCH1516_ALE	855629 =	126 (1.140)	3 (0.050) +TCCCATTGGCATTTACGAAGTTTAAATGTGCAATTAGAATATATGTATAACAATATT	3/162	12.5	4.3%	intergenic (+160/‑600)	zipA_1/cggR	Cell division protein ZipA/Central glycolytic genes regulator
	?	USA300TCH1516_ALE	2830190 =	104 (0.940)					intergenic (+18/+429)	icaC/lipA_2	putative poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine export protein/Lipase 1
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCAC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  USA300TCH1516_ALE/855672‑855629 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AAACACGCGGTGGGCGAAATCAGTTTGAATTGACTGACTTCGTTTT  >  USA300TCH1516_ALE/2830190‑2830235                                             |||||||||||||||||||||||||||||||||||||||||||||||||||||||||                                               GGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCATTGGCATTTACGAAGTTTAAATGTGCAATTAGAATATATGTATAACAATATTAAACACGCGGTGGGCCAAATCAGTTTGAATTGACTGACTT        <  1:810884/141‑1     CGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCATTGGCATTTACGAAGTTTAAATGTGCAATTAGAATATATGTATAACAATATTAAACACGCGGTGGGCGAAATCAGTTTGAATTGACTGACTTCGTT    <  2:144670/141‑1       ACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCATTGGCATTTACGAAGTTTAAATGTGCAATTAGAATATATGTATAACAATATTAAACACGCGGTGGGCGAAATCAGTTTGAATTGACTGACTTCGTTTT  <  1:208338/141‑1                                             |||||||||||||||||||||||||||||||||||||||||||||||||||||||||                                               GGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCAC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  USA300TCH1516_ALE/855672‑855629 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AAACACGCGGTGGGCGAAATCAGTTTGAATTGACTGACTTCGTTTT  >  USA300TCH1516_ALE/2830190‑2830235

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.