New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_bme_pgi	1686709 =	40 (0.870)	4 (0.090)	4/272	6.3	9.0%	intergenic (‑121/+22)	fumC/fumA	fumarate hydratase (fumarase C),aerobic Class II/fumarate hydratase (fumarase A), aerobic Class I
	?	NC_000913_3_bme_pgi	1686725 =	43 (0.980)					intergenic (‑137/+6)	fumC/fumA	fumarate hydratase (fumarase C),aerobic Class II/fumarate hydratase (fumarase A), aerobic Class I
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

ATCTTTTTCGCTGCGTACTGTATTCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTAGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGAAGGGCGGCTCTGTTTATTTCACACAGCGGGTGCATTGTGTGAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATCTTCCACTTCAATTTTCCAGATGGCTTCCATTCC  >  NC_000913_3_bme_pgi/1686562‑1686853                                                                                                                                                    |                                                                                                                                                 aTCTTTTTCGCTGCGTACTGTATTCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTAGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAAcc                                                                                                                                                 <  2:24603/149‑1 (MQ=255)         cGCTGCGTACTGTATTCATGACCTGCTCCTCCCCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTAGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGaa                                                                                                                                         <  2:33690/149‑1 (MQ=255)            tGCGTACTGTATTCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTAGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGAAggg                                                                                                                                      <  1:417879/149‑1 (MQ=255)             gCGTACTGTATTCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTAGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGAAGggc                                                                                                                                     <  2:529644/149‑1 (MQ=255)                   tGTATTCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTAGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGAAGGGCGGctct                                                                                                                               >  2:267163/1‑149 (MQ=255)                        tCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTAGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGAAGGGCGGCTCTGTTTa                                                                                                                          <  1:366816/149‑1 (MQ=255)                         cATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTAGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACGGCCCCGAAGGGCGGCTCTGTTTAt                                                                                                                         <  1:701910/149‑1 (MQ=255)                          aTGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTAGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGAAGGGCGGCTCTGTTTAtt                                                                                                                        >  2:101515/1‑149 (MQ=255)                               cTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTAGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAACAAAACCGCCCCGAAGGGCGGCTCTGTTTATTTccca                                                                                                                   >  1:515653/1‑149 (MQ=255)                               cTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTAGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGAAGGGCGGCTCTGTTTATTTcaca                                                                                                                   <  1:251628/149‑1 (MQ=255)                               cTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTAGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGAAGGGCGGCTCTGTTTATTTcaca                                                                                                                   >  2:251628/1‑149 (MQ=255)                                      tcACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTGTTAGCTCTTTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTTCGATCTAAAAAACCCGCCCCGAAGGGCGGCTCTGTCTAATTCCAACAGCggg                                                                                                            >  2:87950/1‑149 (MQ=255)                                            gATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTAGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGAAGGGCGGCTCTGTTTATTTCACACAGCGGGTGCAtt                                                                                                      <  1:647635/149‑1 (MQ=255)                                                  ttttttCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTAGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCCTGCTTGTTATAAAAACCGCCCCGAAGGGCGGCTCTGTTTATTTCACACAGCGGGTGCATTGTGTGa                                                                                                >  1:594421/1‑149 (MQ=255)                                                             tGTTTTGCTTTCGTTAAGCAACTTTTAGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGAAGGGGGGCTCTGTTTCTTTCACACAGCGGGTGCATGGGGTGAGGTGTAGctgc                                                                                     >  1:248010/1‑149 (MQ=255)                                                               ttttGCTTTCGTTAAGCAACTTTTAGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGAAGGGCGGCTCTGTTTATTTCACACAGCGGGTGCATTGTGTGAGTTGTATctgctg                                                                                   >  1:637172/1‑149 (MQ=255)                                                               ttttGCTTTCGTTAAGCAACTTTTAGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTATTTCGTGCCATGTAAAAAAACCGCCCCGAAGGGCGGCTATGTTTATTTCACACAGCGGGTGCATTGGTTGAGTTGTAGctgctg                                                                                   >  1:695224/1‑149 (MQ=255)                                                               ttttGCTTGTGTTAAGCAACTTTTAGCTCAGTTCTTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTACCAAAACCGCCCCGAAGGGCGGCTCTGTTTATTTCACACAGCGGGTGCATTGTGTGAGTTGTATctgctg                                                                                   <  2:220959/149‑1 (MQ=255)                                                                     tttCGTTAAGCAACTTTTAGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATTTAATAAAACCGCCCCGAAGGGCGGCTCTGTTTATTTCACACAGCGGGTGCATTGTGTGAGTTGTATCTGCTGgaagaa                                                                             >  2:725710/1‑149 (MQ=255)                                                                        cGTTAAGCAACTTTTAGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGAAGGGCGGCTCTGTTTATTTCACACAGCGGGTGCATTGTGTGAGTTGTATCTGCTGGAAGAAGTc                                                                          <  2:111052/149‑1 (MQ=255)                                                                             aGCAACTTTTAGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGAAGGGCGGCTCTGTTTATTTCACACAGCGGGTGCATTGTGTGAGTTGTATCTGCTGGAAGAAGTCATTTc                                                                     <  1:101515/149‑1 (MQ=255)                                                                                aaCTTTTAGCTCACTTATTAATTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGAAGGGCGGCTCTGTTTATTTCACACAGCGGGTGCATTGTGTGAGTTGTATCTGCTGGAAGAAGTCATTTCCtt                                                                  <  2:586279/149‑1 (MQ=255)                                                                                     ttAGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGAAGGGCGGCTCTGTTTATTTCACACAGCGGGTTCATTGTGTGAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTatca                                                             >  1:169445/1‑149 (MQ=255)                                                                                           cACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGAAGGGCGGCTCTGTTTATTTCACACAGCGGGTGCATTGTGTGAGtt                                                                                             >  2:196026/1‑111 (MQ=255)                                                                                           cACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGAAGGGCGGCTCTGTTTATTTCACACAGCGGGTGCATTGTGTGAGtt                                                                                             <  1:196026/111‑1 (MQ=255)                                                                                              ttattaTTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGAAGGGCGGCTCTGTTTATTTCACACAGCGGGTGCATTGTGTGAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAgg                                                    >  1:416194/1‑149 (MQ=255)                                                                                                  tattTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGAAGGGCGGCTCTGTTTATTTCACACAGCGGGTGCATTGTGTGAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATaa                                                >  1:18047/1‑149 (MQ=255)                                                                                                  tattTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGAAGGGCGGCTCTGTTTATTTCACACAGCGGGTGCATTGTGTGAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATaa                                                >  2:324305/1‑149 (MQ=255)                                                                                                        ccATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGAAGGGCGGCTCTGTTTATTTCACACAGCGTGTGCATTGTGTGAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATAAACGCCg                                          <  1:267163/149‑1 (MQ=255)                                                                                                                    aaaTGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGAAGGGCGGCTCTGTTTATTTCACACAGCGGGTGCATTGTGTGAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATCTTCCa                              <  2:637172/149‑1 (MQ=255)                                                                                                                     aaTGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGAAGGGCGGCTCTGTTTATTTCACACAGCGGGTGCATTGTGTGAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATCTTCCAc                             <  1:324305/149‑1 (MQ=255)                                                                                                                          ttGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGAAGGGCGGCTCTGTTTATTTCACACAGCGGGTGCATTGTGTGATTGGTATctgct                                                                                    <  2:300660/89‑1 (MQ=255)                                                                                                                          ttGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGAAGGGCGGCTCTGTTTATTTCACACAGCGGGTGCATTGTGTGAGTTGTATctgct                                                                                    >  1:300660/1‑89 (MQ=255)                                                                                                                            gctCTTTCGTGCCATGTAGAAAGACAGTCCCGAAGGGCGGCTTCGTTTATTTCACACGGCGGGTGCATTGTGTGAGTTGTATCTGCTGCAAGAACTCATTTCCTTTCTCATCCACAAGGATAAACGCCGCGAAATCTTCCACTTCAAtt                      <  2:348085/147‑1 (MQ=255)                                                                                                                              tCTTTCGTGCCATGTAAAAAAACCGCCCCGAAGGGCGGCTCTGTTTATTTCACACAGCGGGTGCATTGTGTGAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATCTTCCACTTCAAtttt                    >  1:173585/1‑149 (MQ=255)                                                                                                                                 ttCGTGCCATGTAAAAAAACCGCCCCGAAGGGCGGCTCTGTTTATTTCACACAGCGGGTGCATTGTGTGAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATCTTCCACTTCAATTTTCCa                 <  1:773101/149‑1 (MQ=255)                                                                                                                                  tCGTGCCATGTAAAAAAACCGCCCCGAAGGGCGGCTCTGTTTATTTCACACAGCGGGTGCATTGTGTGAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATCTTCCACt                            <  2:709024/137‑1 (MQ=255)                                                                                                                                  tCGTGCCATGTAAAAAAACCGCCCCGAAGGGCGGCTCTGTTTATTTCACACAGCGGGTGCATTGTGTGAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATCTTCCACt                            >  1:709024/1‑137 (MQ=255)                                                                                                                                    gtaccATGTAACAAAACGGCCCTGACGGGCGGCTCTGTTTATTTCGCACCGCGGGTGTATTGTGTGAGTTGTATCTTCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATAAACGCCGGGAGATCTTCCACTTCAATTTTCCAGAt              <  2:484836/146‑1 (MQ=255)                                                                                                                                                aaaaCCGCCCCGAAGGGCGGCTCTGTTTATTTCACACAGCGGGTGCATTGTGTGAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAAACTTCCCCTTCAATTTTCCAGATGGCTTCCATTcc  >  2:263112/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                 ATCTTTTTCGCTGCGTACTGTATTCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTAGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGAAGGGCGGCTCTGTTTATTTCACACAGCGGGTGCATTGTGTGAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATCTTCCACTTCAATTTTCCAGATGGCTTCCATTCC  >  NC_000913_3_bme_pgi/1686562‑1686853

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.