New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_bme_pgi = 314062942 (0.910)4 (0.090) 4/268 6.2 8.6% coding (171/489 nt) hybE hydrogenase 2‑specific chaperone
?NC_000913_3_bme_pgi = 3140635 45 (1.040)coding (165/489 nt) hybE hydrogenase 2‑specific chaperone
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TATTGCGAAACACCGTCCAGTTCGCCAACGGTAAAGGTCATAGTGCCATACGGCAGTTGCAGACCAATTTTTTCACTCACTTTGCGCAGCGGCCAGAGTTGATCCGGGCCGGGGAAGATAACTGCACTCAGCATCCACGGGGTGATCACACACCCCGTCCACTGACCTTCGAACAGCGTAAAATCAGAAACATACACCGGCATTGAAGGATGCAGAAAAGAGAGATCGTGCATCGAACGCCGGGCAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTG  >  NC_000913_3_bme_pgi/3140486‑3140775
                                                                                                                                               |                                                                                                                                                  
tatTGCGAAACACCGTCCAGTTCGCCAACGGTAAAGGTCATAGTGCCATACGGCAGTTGCAGACCAATTTTTTCACTCACTTTGCGCAGCGGCCAGAGTTGATCCGGGCCGGGGAAGATAACTGCACTCAGCATCCACGGGGTGATcac                                                                                                                                               >  1:560258/1‑149 (MQ=255)
   tGCGAAATACCGTCTAGGACGGTAACGGTAAAGGTCATAGTGCCATACGGCAGGTGTAGACCAATGTTGTCACTCACTTTGCGCAGCGGCCAGAGTTCATCCGGGCCGGCGAAGATAACTGCACTCAGCATCCACGGGGTGATcacaca                                                                                                                                            <  2:533007/149‑1 (MQ=255)
         acacCGTCCAGTTCGCCAACGGTAAAGGTCATAGTGCCATACGGCAGTTGCAGACCAATTTTTTCACTCACTTTGCGCAGCGGCCAGAGTTGATCCGGGCCGGGGAAGATAACTGCACTCAGCATCCACGGGGTGATCACACACCCCGt                                                                                                                                      >  2:536723/1‑149 (MQ=255)
                    ttCGCCCACGGTAAAGGTCATAGTGCCATACGGCAGTTGCAGACCAATTTTTTCACTCACTTTGCGCAGCGGCCAGAGTTGATCCGGGCCGGGGAAGATAACTGCACTCAGCATCCACGGGGTGATCACAAACCCCGTCCACTGAccct                                                                                                                           >  1:784488/1‑147 (MQ=255)
                     tggcaaaCGGTAAAGGTCAGAGTGCCATACGGCAGTTGCAGACCAATTTTTGCACTCACTTTGCGCAGCGGCCAGAGTTGATCCGGGCCGGGGAAGATAACTGCACTCAGCATCCACGGGGTGATCACACACCCCGTCCACTGACCTTc                                                                                                                          <  2:196774/144‑1 (MQ=255)
                           aCGGTAAAGGTCATAGTGCCATACGGCAGTTGCAGACCAATTTTTTCACTCACTTTGCGCAGCGGCCAGAGTTGATCCGGGCCGGGGAAGATAACTGCACTCAACATCCACGGGTTGATCACACACCCCGTCCACTGACCTTCGAACAg                                                                                                                    >  1:755887/1‑149 (MQ=255)
                            ccgTAAACGTCATAGTGCCATCCGGCAGTTGCAGACCCATTTTTCCCCTCACTTTGCGCAGCGGCCAGAGTTGATCCGGGCCGGGGAAGATAACTGCACTCAGCATCCACGGGGTGATCACACACCCCGTCCACTGACCTTCGAACAGc                                                                                                                   <  1:51012/147‑1 (MQ=255)
                                    gTCATAGTGCCATACGGCAGTTGCAGACCAATTTTTTCACTCACTTTGCGCAGCGGCCAGAGTTGATCCGGGCCGGGGAAGATAACTGCACTCAGCATCCACGGGGTGATCACACACCCCGTCCACTGACCTTCGACCAGCGTAAAATc                                                                                                           >  1:352661/1‑149 (MQ=255)
                                     tCACAGTGCCAGACGGCAGTTGCAGACCAATTTTTTCACTCACTTTGCGCAGCGGCCAGAGTTGATCCGGGCCGGGGAAGATAACTGCACTCAGCATCCACGGGGTGATCACACACCCCGTCCACTGACCTTCGAACAGCGTAAAATCa                                                                                                          <  1:438718/149‑1 (MQ=255)
                                          gTGCCATACGGCAGTTGCAGACCAATTTTTTCCCTCACTTTGCGCAGCGGCCAGAGTTGATCCGGGCCGGGGAAGATAACTGCACTCAGCATCCACGGGGTGATCACACACCCCGTCCACTGACCTTCGAACAGCGTAAAATCAGAAAc                                                                                                     <  1:173597/149‑1 (MQ=255)
                                           tGCCATACGGCAGTTGCAGACCAATTTTTTCACTCACTTTGCGCAGCGGCCAGAGTTGATCCGGGCCGGGGAAGATAACTGCACTCAGCATCCACGGGGTGATCACACACCCCGTCCACTGACCTTGTAACAGGTTAAAATCAAAAAcc                                                                                                    >  1:46051/1‑148 (MQ=255)
                                           tGCCATACGGCAGTTGCAGACCAATTTTTTCACTCACTTTGCGCAGCGCCGAGGGTGGAGCCGGGCCGGGGAAGATAACTGCACTCAGCATCCACGGGGTGATCACACACCCCGTCCACTGCCCTTCGAACAGCGTCACATCAGAAACa                                                                                                    >  2:339052/1‑149 (MQ=255)
                                             ccATACGGCAGTTGCAGACCAATTTTTTCACTCACTTTGCGCAGCGGCCAGAGTTGATCCGGGCCGGGGAAGATAACTGCACTCAGCATCCACGGGGTGATCACACACCCCGTCCACTGACCTTCGAACAGCGTAAAATCAGAAACATa                                                                                                  >  1:319246/1‑149 (MQ=255)
                                               aTACGGCAGTTGCAGACCAATTTTTTCACTCACTTAGCGCAGCGGCCAGCGTAGATCCGGGACGGGGCCGATTCATGCACTCAGCATCCACGGGGAGATCACCAACCCCGACCACTGACCTTCGAACACCGTGACATCCGCAACCTaca                                                                                                >  2:447843/1‑149 (MQ=255)
                                                          gCAGACCAATTTTTTCACTCACTTTGCGCAGCGGCCAGCGTTGATCCGGGCCGGGGAAGATAACTGCACTCAGCATCCACGGGGTGATCACACACCCCGTCCACTGACCTGCGAACAGCGTAAAATCAGAAACATACACCGGCATTGaa                                                                                     <  1:310484/149‑1 (MQ=255)
                                                          gCAGACCAATTTTTTCACTCACGTTGCGCCGCCATACGAATCTATCCGGGCCGGTGAAGATAACTGCACTCCAAATGGATGTTTTTATTGCGCTGCGCGTCCACTGTCCTTCGACCAGCGTAAAATCAGAAACATACACCGGCATTGaa                                                                                     <  2:536361/149‑1 (MQ=255)
                                                            aGACCAATTTTTTCACTCACTTTGCGCAGCGGCCAGAGTTGATCCGAGCCGGGGAAGATAACTGCACTCAGCATCCACGGGGTGATCACACACCCCGTCCACTGACCTTCGAACAGCGTAAAATCAGAAACATACACCGGCATTGAAgg                                                                                   >  1:731815/1‑149 (MQ=255)
                                                               ccAATTTTTTCACCCACTTTGCGCAGCGGCCAGAGTTGATCCGGGCCGGGGAAGATAACTGCACTCAGCATCCACGGGGTGATCACACACCCCGTCCACTGACCTTCGAACAGCGTAAAATCAGAAACATACACCGGCATTGAAGGATg                                                                                <  1:259533/149‑1 (MQ=255)
                                                                cAATTTTTTCACTCACTTTGCGCAGCGGCCAGAGTTGATCCGGGCCGGGGAAGATAACTGCACTCAGCATCCACGGGGTGATCACACACCCCGTCCACTGACCTTCGAACAGCGTAAAATCAGAAACATACACCGGCATTGAAgg                                                                                   <  1:592575/145‑1 (MQ=255)
                                                                cAATTTTTTCACTCACTTTGCGCAGCGGCCAGAGTTGATCCGGGCCGGGGAAGATAACTGCACTCAGCATCCACGGGGTGATCACACACCCCGTCCACTGACCTTCGAACAGCGTAAAATCAGAAACATACACCGGCATTGAAgg                                                                                   >  2:592575/1‑145 (MQ=255)
                                                                         ccctcactTTGCGCAGCGGCCAGAGTTGATCCGGGCCGGGGAAGATAACTGCACTCAGCATCCACGGGGTGATCACACACCCCGTCCACTGACCTTCGAACAGCGTAAAATCAGAAACATACACCGGCATTGAAGGATGCAGAAAagag                                                                      <  1:680021/147‑1 (MQ=255)
                                                                          actcactTTGCGCAGCGGCCAGAGTTGATCCGGGCCGGGGAAGATAACTGCACTCAGCATCCACGGGGTGATCACACACCCCGTCCACTGACCTTCGAACAGCGTAAAATCAGAAACATACACCGGCATTGAAGGATGCAGAAAagaga                                                                     <  1:298005/149‑1 (MQ=255)
                                                                          actcactTTGCGCAGCGGCCAGAGTTGATCCGGGCCGGGGAAGATAACTGCACTCAGCATCCACGGGGTGATCACACACCCCGTCCACTGACCTTCGAACAGCGTAAAATCAGAAACATACACCGGCATTGAAGGATGCAAAAAagaaa                                                                     >  2:757358/1‑147 (MQ=255)
                                                                                tttGCGCAGCGGCCAGAGTTGATCCGGGCCGGGGAAGATAACTGCACTCAGCATCCACGGGGTGATCACACACCCCGTCCACTGACCTTCGAACAGCGTAAAATCAGAAACATACACCGGCATTGAAGGATGCAGAAAAGAGAGATCGt                                                               >  1:42660/1‑149 (MQ=255)
                                                                                tttGCGCAGCGGCCAGAGTTGATCCGGGCCGGGGAAGATAACTGCACTCAGCATCCACGGGGTGATCACACACCCCGTCCACTGACCTTCGAACAGCGTAAAATCAGAAACATACACCGGCATTGAAGGATGCAGAAAAGAGAGATCGt                                                               <  2:35842/149‑1 (MQ=255)
                                                                                        gCGGCCAGGTGTGATCTGGTCCGGGGAAGATAACTGCACTCAGCATCCACGGGGTGATCACACACCCCGTCCACTGACCTTCGAACAGCGTAAAATCAGAAACATACACCGGCATTGCAGGATGCAGAAAAGAGAGATCGTGCATCGaa                                                       <  2:552794/149‑1 (MQ=255)
                                                                                                  ttGATCCGGGCCGGGGAAGATAACTGCACTCAGCATCCACGGGGTGATCACACACCCCGTCCACTGACCTTCGAACAGCGTAAAATCAGAAACATACACCGGCATTGAAGGATGCAGAAAAGAGAGATCGTGCATCGAACGCCGGGCaa                                             >  1:775171/1‑149 (MQ=255)
                                                                                                     atcgaggccggaGAAGATAACTGCACTCAGCTTCCACGGGTTGATCACACACCCCGTCCACTGACCTTCGAACAGCGTAAAATCAGAAACATACACCGGCATTGAAGGATGCAGAAAAGAGAGATCGTGCATCGAACGCCGGGCAAttt                                          >  2:600923/6‑149 (MQ=255)
                                                                                                     aTCCGGGCCGGGGAAGATAACTGCACTCAGCATCCACGGGGTGATCACACACCCCGTCCACTGACCTTCGAACCGCGTAAAATCAGAAACATACACCGGCATTGAAGGATGCAGAAAAGAGAGATCGTGCATCGAACGCCGGGCAAttt                                          >  2:62934/1‑149 (MQ=255)
                                                                                                      tCCGGGCCGGGGAACATAACCGCACCCAGCATCCCCGGGGTGATCATACAGCGCTTCCTCTGACCTTCGAACAGCGTAAAATCAGAAACATACGCCGGCATTGAAGGATGCAGAAAAGAGAGATCGTGCATCGAACGCCGGGCAATttc                                         <  1:514405/149‑1 (MQ=255)
                                                                                                            ccgggGAAGATAACTGCACTCAGCATCCACGGGGTGATCACACACCCCGTCCACTGACCTTCGAACAGCGTAAAATCAGAAACATACACCGGCATTGAAGGATGCAGAAAAGAGAGATCGTGCATCGAACGCCGGGCAATTTCTTCaaa                                   <  1:536723/149‑1 (MQ=255)
                                                                                                                ggAAGATAACTGCACTCAGCATCCACGGGGTGATCACACACCCCGTCCACTGACCTTCGAACAGCGTAAAATCAGAAACATACACCGGCATTGAAGGATGCAGCAAAGAGAGATCGTGCTTCGAACGCCGGGCAATTTCTTCAAACGCt                               <  2:731815/149‑1 (MQ=255)
                                                                                                                    gATAACTGCACTCAGCATCCACGGGGTGATCACACACCCCGTCCACTGACCTTCGAACAGCGTAAAATCAGAAACATACACCGGCATTGAAGGATGCAGAAAAGAGAGATCGTGCATCGAACGCCGGGCAATTTCTTCAAACGCTGCCt                           >  2:291888/1‑149 (MQ=255)
                                                                                                                       aaCTGCACTCAGCATCCACGGGGTGATCACACACCCCGTCCACTGACCTTCGAACAGCGTAAAATCAGAAACATACACCGGCATTGAAGGATGCAGAAAAGAGAGATCGTGCATCGAACGCCGGGCACTTTCTTCAAACGCTGCCTGTa                        >  1:491939/1‑149 (MQ=255)
                                                                                                                            gaCTCAGCATGCACGGGGTGATCACACACCCCGTCGACTGACCTTCGAACAGCGTAAAATCAGTCACCTCAGCAGGCCGAGCAGTAGTCAGAAAAGTGAGTTGCTCCATCGAACGCCGGGAATTCACTCCAAACGGTGGTTGTACTTgc                   <  2:375607/148‑1 (MQ=255)
                                                                                                                               tCAGCATCCACGGGGTGATCACACACCCCGTCCACTGACCTTCGAACAGCGTAAAATCAGAAACATACACCGGCATTGAAGGATGCAGAAAAGAGAGATCGTGCATCGAACGCCGGGCAATTTCTTCAAACGCTGCCTGTACTTGCGcc                >  2:638828/1‑149 (MQ=255)
                                                                                                                               tCAGCATCCACGGGGTGATCACACACCCCGTCCACTGACCTTCGAACAGCGTAAAATCAGAAACATACACCGGCATTGAAGGATGCAGAAAAGAGAGATCGTGCATCGAACGCCGGGCAATTTCTTCAAACGATGCCTGTACTTGCGcc                >  1:537965/1‑149 (MQ=255)
                                                                                                                                    aTCCACGGGGTGATCACACACCCCGTCCACTGACCTTCGAACAGCGTAAAATCAGAAACATACACCGGCATTGAAGGATGCAGAAAAGAGAGATCGTGCATCGAACGCCGGGCAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCgg           <  1:317417/149‑1 (MQ=255)
                                                                                                                                    aTCCACGGGGTGATCACACACCCCGTCCACTGACCTTCGAACAGAGAACAGTCAGAAGCATACACCGGCATTGAAGGATGCAGAAAAGAGAGATCGTGCATCGAACGCCGGGCAATTTCTTCAAACGCTGCCTGTACTTGCGCCCTCgg           >  1:763814/1‑149 (MQ=255)
                                                                                                                                        aCGGGGTGATCACACACCCCGTCCACTGACCTTCGAACAGCGTAAAATCAGAAACATACACCGGCATTGAAGGATGCAGAAAAGAGAGATCGTGCATCGAACGCCGGGCAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAg       <  1:291888/149‑1 (MQ=255)
                                                                                                                                           gggTGATCACACACCCCGTCCACTGACCTTCGAACAGCGTAAAATCAGAAACATACACCGGCATTGAAGGATGCAGAAAAGAGAGATCGTGCATCGAACGCCGGGCAATTTCTTCAAACGCTGCCTGTACTTGCGCCGTCGGGGAGGTc    >  2:36060/1‑149 (MQ=255)
                                                                                                                                             gTGATCACACACCCCGTCCACTGACCTTCGAACAGCGTAAAATCAGAAACATACACCGGCATTGAAGGATGCAGAAAAGAGAGATCGTGCATCGAACGCCGGGCAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTg  <  1:723476/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                  
TATTGCGAAACACCGTCCAGTTCGCCAACGGTAAAGGTCATAGTGCCATACGGCAGTTGCAGACCAATTTTTTCACTCACTTTGCGCAGCGGCCAGAGTTGATCCGGGCCGGGGAAGATAACTGCACTCAGCATCCACGGGGTGATCACACACCCCGTCCACTGACCTTCGAACAGCGTAAAATCAGAAACATACACCGGCATTGAAGGATGCAGAAAAGAGAGATCGTGCATCGAACGCCGGGCAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTG  >  NC_000913_3_bme_pgi/3140486‑3140775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.