New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_bme_pgi	= 3140629	42 (0.910)	4 (0.090)	4/268	6.2	8.6%	coding (171/489 nt)	hybE	hydrogenase 2‑specific chaperone
	?	NC_000913_3_bme_pgi	= 3140635	45 (1.040)					coding (165/489 nt)	hybE	hydrogenase 2‑specific chaperone
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TGCGAAACACCGTCCAGTTCGCCAACGGTAAAGGTCATAGTGCCATACGGCAGTTGCAGACCAATTTTTTCACTCACTTTGCGCAGCGGCCAGAGTTGATCCGGGCCGGGGAAGATAACTGCACTCAGCATCCACGGGGTGATCACACACCCCGTCCACTGACCTTCGAACAGCGTAAAATCAGAAACATACACCGGCATTGAAGGATGCAGAAAAGAGAGATCGTGCATCGAACGCCGGGCAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTG  >  NC_000913_3_bme_pgi/3140489‑3140783                                                                                                                                                   |                                                                                                                                                     tGCGAAATACCGTCTAGGACGGTAACGGTAAAGGTCATAGTGCCATACGGCAGGTGTAGACCAATGTTGTCACTCACTTTGCGCAGCGGCCAGAGTTCATCCGGGCCGGCGAAGATAACTGCACTCAGCATCCACGGGGTGATcacaca                                                                                                                                                    <  2:533007/149‑1 (MQ=255)       acacCGTCCAGTTCGCCAACGGTAAAGGTCATAGTGCCATACGGCAGTTGCAGACCAATTTTTTCACTCACTTTGCGCAGCGGCCAGAGTTGATCCGGGCCGGGGAAGATAACTGCACTCAGCATCCACGGGGTGATCACACACCCCGt                                                                                                                                              >  2:536723/1‑149 (MQ=255)                  ttCGCCCACGGTAAAGGTCATAGTGCCATACGGCAGTTGCAGACCAATTTTTTCACTCACTTTGCGCAGCGGCCAGAGTTGATCCGGGCCGGGGAAGATAACTGCACTCAGCATCCACGGGGTGATCACAAACCCCGTCCACTGAccct                                                                                                                                   >  1:784488/1‑147 (MQ=255)                   tggcaaaCGGTAAAGGTCAGAGTGCCATACGGCAGTTGCAGACCAATTTTTGCACTCACTTTGCGCAGCGGCCAGAGTTGATCCGGGCCGGGGAAGATAACTGCACTCAGCATCCACGGGGTGATCACACACCCCGTCCACTGACCTTc                                                                                                                                  <  2:196774/144‑1 (MQ=255)                         aCGGTAAAGGTCATAGTGCCATACGGCAGTTGCAGACCAATTTTTTCACTCACTTTGCGCAGCGGCCAGAGTTGATCCGGGCCGGGGAAGATAACTGCACTCAACATCCACGGGTTGATCACACACCCCGTCCACTGACCTTCGAACAg                                                                                                                            >  1:755887/1‑149 (MQ=255)                          ccgTAAACGTCATAGTGCCATCCGGCAGTTGCAGACCCATTTTTCCCCTCACTTTGCGCAGCGGCCAGAGTTGATCCGGGCCGGGGAAGATAACTGCACTCAGCATCCACGGGGTGATCACACACCCCGTCCACTGACCTTCGAACAGc                                                                                                                           <  1:51012/147‑1 (MQ=255)                                  gTCATAGTGCCATACGGCAGTTGCAGACCAATTTTTTCACTCACTTTGCGCAGCGGCCAGAGTTGATCCGGGCCGGGGAAGATAACTGCACTCAGCATCCACGGGGTGATCACACACCCCGTCCACTGACCTTCGACCAGCGTAAAATc                                                                                                                   >  1:352661/1‑149 (MQ=255)                                   tCACAGTGCCAGACGGCAGTTGCAGACCAATTTTTTCACTCACTTTGCGCAGCGGCCAGAGTTGATCCGGGCCGGGGAAGATAACTGCACTCAGCATCCACGGGGTGATCACACACCCCGTCCACTGACCTTCGAACAGCGTAAAATCa                                                                                                                  <  1:438718/149‑1 (MQ=255)                                        gTGCCATACGGCAGTTGCAGACCAATTTTTTCCCTCACTTTGCGCAGCGGCCAGAGTTGATCCGGGCCGGGGAAGATAACTGCACTCAGCATCCACGGGGTGATCACACACCCCGTCCACTGACCTTCGAACAGCGTAAAATCAGAAAc                                                                                                             <  1:173597/149‑1 (MQ=255)                                         tGCCATACGGCAGTTGCAGACCAATTTTTTCACTCACTTTGCGCAGCGGCCAGAGTTGATCCGGGCCGGGGAAGATAACTGCACTCAGCATCCACGGGGTGATCACACACCCCGTCCACTGACCTTGTAACAGGTTAAAATCAAAAAcc                                                                                                            >  1:46051/1‑148 (MQ=255)                                         tGCCATACGGCAGTTGCAGACCAATTTTTTCACTCACTTTGCGCAGCGCCGAGGGTGGAGCCGGGCCGGGGAAGATAACTGCACTCAGCATCCACGGGGTGATCACACACCCCGTCCACTGCCCTTCGAACAGCGTCACATCAGAAACa                                                                                                            >  2:339052/1‑149 (MQ=255)                                           ccATACGGCAGTTGCAGACCAATTTTTTCACTCACTTTGCGCAGCGGCCAGAGTTGATCCGGGCCGGGGAAGATAACTGCACTCAGCATCCACGGGGTGATCACACACCCCGTCCACTGACCTTCGAACAGCGTAAAATCAGAAACATa                                                                                                          >  1:319246/1‑149 (MQ=255)                                             aTACGGCAGTTGCAGACCAATTTTTTCACTCACTTAGCGCAGCGGCCAGCGTAGATCCGGGACGGGGCCGATTCATGCACTCAGCATCCACGGGGAGATCACCAACCCCGACCACTGACCTTCGAACACCGTGACATCCGCAACCTaca                                                                                                        >  2:447843/1‑149 (MQ=255)                                                        gCAGACCAATTTTTTCACTCACTTTGCGCAGCGGCCAGCGTTGATCCGGGCCGGGGAAGATAACTGCACTCAGCATCCACGGGGTGATCACACACCCCGTCCACTGACCTGCGAACAGCGTAAAATCAGAAACATACACCGGCATTGaa                                                                                             <  1:310484/149‑1 (MQ=255)                                                        gCAGACCAATTTTTTCACTCACGTTGCGCCGCCATACGAATCTATCCGGGCCGGTGAAGATAACTGCACTCCAAATGGATGTTTTTATTGCGCTGCGCGTCCACTGTCCTTCGACCAGCGTAAAATCAGAAACATACACCGGCATTGaa                                                                                             <  2:536361/149‑1 (MQ=255)                                                          aGACCAATTTTTTCACTCACTTTGCGCAGCGGCCAGAGTTGATCCGAGCCGGGGAAGATAACTGCACTCAGCATCCACGGGGTGATCACACACCCCGTCCACTGACCTTCGAACAGCGTAAAATCAGAAACATACACCGGCATTGAAgg                                                                                           >  1:731815/1‑149 (MQ=255)                                                             ccAATTTTTTCACCCACTTTGCGCAGCGGCCAGAGTTGATCCGGGCCGGGGAAGATAACTGCACTCAGCATCCACGGGGTGATCACACACCCCGTCCACTGACCTTCGAACAGCGTAAAATCAGAAACATACACCGGCATTGAAGGATg                                                                                        <  1:259533/149‑1 (MQ=255)                                                              cAATTTTTTCACTCACTTTGCGCAGCGGCCAGAGTTGATCCGGGCCGGGGAAGATAACTGCACTCAGCATCCACGGGGTGATCACACACCCCGTCCACTGACCTTCGAACAGCGTAAAATCAGAAACATACACCGGCATTGAAgg                                                                                           >  2:592575/1‑145 (MQ=255)                                                              cAATTTTTTCACTCACTTTGCGCAGCGGCCAGAGTTGATCCGGGCCGGGGAAGATAACTGCACTCAGCATCCACGGGGTGATCACACACCCCGTCCACTGACCTTCGAACAGCGTAAAATCAGAAACATACACCGGCATTGAAgg                                                                                           <  1:592575/145‑1 (MQ=255)                                                                       ccctcactTTGCGCAGCGGCCAGAGTTGATCCGGGCCGGGGAAGATAACTGCACTCAGCATCCACGGGGTGATCACACACCCCGTCCACTGACCTTCGAACAGCGTAAAATCAGAAACATACACCGGCATTGAAGGATGCAGAAAagag                                                                              <  1:680021/147‑1 (MQ=255)                                                                        actcactTTGCGCAGCGGCCAGAGTTGATCCGGGCCGGGGAAGATAACTGCACTCAGCATCCACGGGGTGATCACACACCCCGTCCACTGACCTTCGAACAGCGTAAAATCAGAAACATACACCGGCATTGAAGGATGCAGAAAagaga                                                                             <  1:298005/149‑1 (MQ=255)                                                                        actcactTTGCGCAGCGGCCAGAGTTGATCCGGGCCGGGGAAGATAACTGCACTCAGCATCCACGGGGTGATCACACACCCCGTCCACTGACCTTCGAACAGCGTAAAATCAGAAACATACACCGGCATTGAAGGATGCAAAAAagaaa                                                                             >  2:757358/1‑147 (MQ=255)                                                                              tttGCGCAGCGGCCAGAGTTGATCCGGGCCGGGGAAGATAACTGCACTCAGCATCCACGGGGTGATCACACACCCCGTCCACTGACCTTCGAACAGCGTAAAATCAGAAACATACACCGGCATTGAAGGATGCAGAAAAGAGAGATCGt                                                                       >  1:42660/1‑149 (MQ=255)                                                                              tttGCGCAGCGGCCAGAGTTGATCCGGGCCGGGGAAGATAACTGCACTCAGCATCCACGGGGTGATCACACACCCCGTCCACTGACCTTCGAACAGCGTAAAATCAGAAACATACACCGGCATTGAAGGATGCAGAAAAGAGAGATCGt                                                                       <  2:35842/149‑1 (MQ=255)                                                                                      gCGGCCAGGTGTGATCTGGTCCGGGGAAGATAACTGCACTCAGCATCCACGGGGTGATCACACACCCCGTCCACTGACCTTCGAACAGCGTAAAATCAGAAACATACACCGGCATTGCAGGATGCAGAAAAGAGAGATCGTGCATCGaa                                                               <  2:552794/149‑1 (MQ=255)                                                                                                ttGATCCGGGCCGGGGAAGATAACTGCACTCAGCATCCACGGGGTGATCACACACCCCGTCCACTGACCTTCGAACAGCGTAAAATCAGAAACATACACCGGCATTGAAGGATGCAGAAAAGAGAGATCGTGCATCGAACGCCGGGCaa                                                     >  1:775171/1‑149 (MQ=255)                                                                                                   atcgaggccggaGAAGATAACTGCACTCAGCTTCCACGGGTTGATCACACACCCCGTCCACTGACCTTCGAACAGCGTAAAATCAGAAACATACACCGGCATTGAAGGATGCAGAAAAGAGAGATCGTGCATCGAACGCCGGGCAAttt                                                  >  2:600923/6‑149 (MQ=255)                                                                                                   aTCCGGGCCGGGGAAGATAACTGCACTCAGCATCCACGGGGTGATCACACACCCCGTCCACTGACCTTCGAACCGCGTAAAATCAGAAACATACACCGGCATTGAAGGATGCAGAAAAGAGAGATCGTGCATCGAACGCCGGGCAAttt                                                  >  2:62934/1‑149 (MQ=255)                                                                                                    tCCGGGCCGGGGAACATAACCGCACCCAGCATCCCCGGGGTGATCATACAGCGCTTCCTCTGACCTTCGAACAGCGTAAAATCAGAAACATACGCCGGCATTGAAGGATGCAGAAAAGAGAGATCGTGCATCGAACGCCGGGCAATttc                                                 <  1:514405/149‑1 (MQ=255)                                                                                                          ccgggGAAGATAACTGCACTCAGCATCCACGGGGTGATCACACACCCCGTCCACTGACCTTCGAACAGCGTAAAATCAGAAACATACACCGGCATTGAAGGATGCAGAAAAGAGAGATCGTGCATCGAACGCCGGGCAATTTCTTCaaa                                           <  1:536723/149‑1 (MQ=255)                                                                                                              ggAAGATAACTGCACTCAGCATCCACGGGGTGATCACACACCCCGTCCACTGACCTTCGAACAGCGTAAAATCAGAAACATACACCGGCATTGAAGGATGCAGCAAAGAGAGATCGTGCTTCGAACGCCGGGCAATTTCTTCAAACGCt                                       <  2:731815/149‑1 (MQ=255)                                                                                                                  gATAACTGCACTCAGCATCCACGGGGTGATCACACACCCCGTCCACTGACCTTCGAACAGCGTAAAATCAGAAACATACACCGGCATTGAAGGATGCAGAAAAGAGAGATCGTGCATCGAACGCCGGGCAATTTCTTCAAACGCTGCCt                                   >  2:291888/1‑149 (MQ=255)                                                                                                                     aaCTGCACTCAGCATCCACGGGGTGATCACACACCCCGTCCACTGACCTTCGAACAGCGTAAAATCAGAAACATACACCGGCATTGAAGGATGCAGAAAAGAGAGATCGTGCATCGAACGCCGGGCACTTTCTTCAAACGCTGCCTGTa                                >  1:491939/1‑149 (MQ=255)                                                                                                                          gaCTCAGCATGCACGGGGTGATCACACACCCCGTCGACTGACCTTCGAACAGCGTAAAATCAGTCACCTCAGCAGGCCGAGCAGTAGTCAGAAAAGTGAGTTGCTCCATCGAACGCCGGGAATTCACTCCAAACGGTGGTTGTACTTgc                           <  2:375607/148‑1 (MQ=255)                                                                                                                             tCAGCATCCACGGGGTGATCACACACCCCGTCCACTGACCTTCGAACAGCGTAAAATCAGAAACATACACCGGCATTGAAGGATGCAGAAAAGAGAGATCGTGCATCGAACGCCGGGCAATTTCTTCAAACGCTGCCTGTACTTGCGcc                        >  2:638828/1‑149 (MQ=255)                                                                                                                             tCAGCATCCACGGGGTGATCACACACCCCGTCCACTGACCTTCGAACAGCGTAAAATCAGAAACATACACCGGCATTGAAGGATGCAGAAAAGAGAGATCGTGCATCGAACGCCGGGCAATTTCTTCAAACGATGCCTGTACTTGCGcc                        >  1:537965/1‑149 (MQ=255)                                                                                                                                  aTCCACGGGGTGATCACACACCCCGTCCACTGACCTTCGAACAGCGTAAAATCAGAAACATACACCGGCATTGAAGGATGCAGAAAAGAGAGATCGTGCATCGAACGCCGGGCAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCgg                   <  1:317417/149‑1 (MQ=255)                                                                                                                                  aTCCACGGGGTGATCACACACCCCGTCCACTGACCTTCGAACAGAGAACAGTCAGAAGCATACACCGGCATTGAAGGATGCAGAAAAGAGAGATCGTGCATCGAACGCCGGGCAATTTCTTCAAACGCTGCCTGTACTTGCGCCCTCgg                   >  1:763814/1‑149 (MQ=255)                                                                                                                                      aCGGGGTGATCACACACCCCGTCCACTGACCTTCGAACAGCGTAAAATCAGAAACATACACCGGCATTGAAGGATGCAGAAAAGAGAGATCGTGCATCGAACGCCGGGCAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAg               <  1:291888/149‑1 (MQ=255)                                                                                                                                         gggTGATCACACACCCCGTCCACTGACCTTCGAACAGCGTAAAATCAGAAACATACACCGGCATTGAAGGATGCAGAAAAGAGAGATCGTGCATCGAACGCCGGGCAATTTCTTCAAACGCTGCCTGTACTTGCGCCGTCGGGGAGGTc            >  2:36060/1‑149 (MQ=255)                                                                                                                                           gTGATCACACACCCCGTCCACTGACCTTCGAACAGCGTAAAATCAGAAACATACACCGGCATTGAAGGATGCAGAAAAGAGAGATCGTGCATCGAACGCCGGGCAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTg          <  1:723476/149‑1 (MQ=255)                                                                                                                                                cacacaCCCCGTCCACTGACCTTCGAACAGCGTAAAATCAGAAACATACACCGGCATTGAAGGATGCAGAAAAGAGAGATCGTGCATCGAACGCCGGGCAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAAc     <  1:342359/149‑1 (MQ=255)                                                                                                                                                cacacaCCCCGTCACCAGACCTTCGAACAGCGTAAAATCAGAAACACACACCGGCATTGAAGGATGCAGAAAAGAGAGATCGTGCCTCGATCGCCGGGCAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAAc     <  2:520167/149‑1 (MQ=255)                                                                                                                                                 acacacCCCGTCCACTGACCTTCGAACAGCGTAAAATCAGAAACATACACCGGCATTGAAGGATGCAGAAAAGAGAGATCGTGCATCGAACGCCGGGCAATTTCTTCAAACGCTGCCTTTACTTTCGCCTTCGGGGAGGTCTGGAAAcc    >  2:398477/1‑149 (MQ=255)                                                                                                                                                  cacacCCCGTCCACTGACCTTCGAACAGCGTAAAATCAGAAACATACACCGGCATTGAAGGATGCAGAAAAGAGAGATCGTGCATCGAACGc                                                            <  1:575799/92‑1 (MQ=255)                                                                                                                                                  cacacCCCGTCCACTGACCTTCGAACAGCGTAAAATCAGAAACATACACCGGCATTGAAGGATGCAGAAAAGAGAGATCGTGCATCGAACGc                                                            >  2:575799/1‑92 (MQ=255)                                                                                                                                                   acacCCCGTCCACTGACCTTCGAACAGCGTAAAATCAGAAACATACACCGGCATTGAAGGATGCAGAAAAGAGAGATCGTGCATCGAACGCCGGGCAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTg  >  1:251281/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                     TGCGAAACACCGTCCAGTTCGCCAACGGTAAAGGTCATAGTGCCATACGGCAGTTGCAGACCAATTTTTTCACTCACTTTGCGCAGCGGCCAGAGTTGATCCGGGCCGGGGAAGATAACTGCACTCAGCATCCACGGGGTGATCACACACCCCGTCCACTGACCTTCGAACAGCGTAAAATCAGAAACATACACCGGCATTGAAGGATGCAGAAAAGAGAGATCGTGCATCGAACGCCGGGCAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTG  >  NC_000913_3_bme_pgi/3140489‑3140783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.