breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
MC JC 257,908 Δ776 bp 100% [crl] [crl]
RA 526,792 A→C 24.9% S1178R (AGC→CGC)  rhsD → Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
RA 526,796 C→G 29.9% A1179G (GCG→GGG)  rhsD → Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
RA 526,800 A→G 26.9% E1180E (GAA→GAG rhsD → Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
RA 1,466,201 T→C 21.4% pseudogene (810/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,527,876 C→T 9.8% intergenic (+724/‑26) yncH → / → rhsE IPR020099 family protein/pseudogene, Rhs family
MC JC 1,978,503 Δ776 bp 100% insB1insA insB1, insA
RA 2,173,361 Δ2 bp 100% pseudogene (1‑2/442 nt) gatC ← pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,555,718 G→A 9.7% A4V (GCA→GTA)  eutK ← putative ethanol utilization carboxysome structural protein
RA 3,440,084:1 +G 65.2% coding (946/990 nt) rpoA ← RNA polymerase, alpha subunit
RA 3,560,455:1 +G 100% intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,741,287 C→T 9.5% C99Y (TGC→TAC)  ysaA ← putative hydrogenase, 4Fe‑4S ferredoxin‑type component
RA 4,235,433 Δ1 bp 100% intergenic (‑2200/‑501) lysC ← / → yjbE lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein
RA 4,296,088 C→T 24.1% intergenic (+266/+376) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,408:1 +CG 100% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913_3_hsa_pgi 365878 366466 589 13 [12] [12] 13 [lacZ]–[lacI] [lacZ],[lacI]
* * ÷ NC_000913_3_hsa_pgi 1528425–1529174 1529230–1529181 8–806 13 [12] [12] 13 rhsE pseudogene, Rhs family
* * ÷ NC_000913_3_hsa_pgi 3423760–3424234 3424524–3424238 5–765 13 [12] [12] 14 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_hsa_pgi 1207790 =4 (0.110)39 (1.260) 34/242 NT 87.1% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913_3_hsa_pgi 1209619 = 8 (0.260)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913_3_hsa_pgi = 12078054 (0.110)23 (0.740) 18/242 NT 80.0% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913_3_hsa_pgi = 1209602 8 (0.260)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913_3_hsa_pgi = 12994980 (0.000)32 (0.940) 30/266 NT 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913_3_hsa_pgi 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913_3_hsa_pgi = 202459639 (1.110)3 (0.090) 3/274 NT 7.6% intergenic (+5/+39) rcsA/dsrB transcriptional regulator of colanic acid capsular biosynthesis/uncharacterized protein
?NC_000913_3_hsa_pgi = 2024624 34 (0.970)intergenic (+33/+11) rcsA/dsrB transcriptional regulator of colanic acid capsular biosynthesis/uncharacterized protein
* ? NC_000913_3_hsa_pgi 2362102 =32 (0.910)3 (0.090) 3/266 NT 7.7% coding (110/783 nt) rhmR putative DNA‑binding transcriptional regulator for the rhm operon
?NC_000913_3_hsa_pgi 2362167 = 41 (1.200)coding (45/783 nt) rhmR putative DNA‑binding transcriptional regulator for the rhm operon