New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_KO_pgi = 2643100126 (0.830)17 (0.120) 16/246 14.3 13.1% intergenic (‑256/+29) rodZ/rlmN MreB assembly cytoskeletal protein/dual specificity 23S rRNA m(2)A2503, tRNA m(2)A37 methyltransferase, SAM‑dependent
?NC_000913_3_KO_pgi = 2643110 112 (0.820)intergenic (‑266/+19) rodZ/rlmN MreB assembly cytoskeletal protein/dual specificity 23S rRNA m(2)A2503, tRNA m(2)A37 methyltransferase, SAM‑dependent
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

AGTATAAGACCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_KO_pgi/2642940‑2643100
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cattacgccacggtTACTTTATGTACCGTGGCGTAATGTTTTATGAATGAAGTATTAAGTTGCAATTGTCTGGTCAATATTAACGGTGCGTTTTTGCTGACTTTAAGGCAGTATGTAACGATGCAACAGTAAGTTAGCCTTAGTGAATGTGGGCTTTGTCACG  <  NC_000913_3_KO_pgi/2643110‑2642962
                                                                                                                                                                                                                                                                                                                      
AGTATAAGACCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTCCATTCATAAAACAT                                                                                                                                                                  >  2:1327402/1‑150
  TATAAGACCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTCAAGCTGCATTCATAAAACATTA                                                                                                                                                                >  1:14736/1‑150
  TATAAGACCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTA                                                                                                                                                                <  1:1305916/150‑1
  TATAAGACCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTA                                                                                                                                                                >  1:212954/1‑150
   ATAAGACCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTAC                                                                                                                                                               >  1:1689571/1‑150
    TAAGACCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTACAACTTAATACATCATTCATAAAACATAACG                                                                                                                                                              >  2:1664194/1‑150
     AAGACCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGC                                                                                                                                                             <  3:178770/150‑1
      AGACCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCC                                                                                                                                                            >  1:747161/1‑150
      AGACCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATAATGCCTTAAAGTAAGAAAAAAAGCACTGTTAATATTGACCAGACAATCGCAACATAATACTTCATACATAAAACATTACGCC                                                                                                                                                            >  1:1684644/1‑150
       GACCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCA                                                                                                                                                           >  2:5862/1‑150
       GACCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCA                                                                                                                                                           >  2:1242872/1‑150
        ACCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCAC                                                                                                                                                          >  1:359403/1‑150
         CCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACG                                                                                                                                                         <  1:1616798/150‑1
         CCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACG                                                                                                                                                         >  1:549553/1‑150
          cttcaaTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGG                                                                                                                                                        <  3:124306/144‑1
          CGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAAGTGCAACTTAATACTTCATTCATAAAACATTACGCCACGG                                                                                                                                                        <  3:552227/150‑1
                             AAGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTTACTTTATGTACCGTGGC                                                                                                                                     <  2:747161/150‑1
                               GCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTTACTTTATGTACCGTG                                                                                                                                       >  4:992991/1‑146
                               GCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTTACTTTATGTACCGTG                                                                                                                                       <  3:992991/146‑1
                                  CACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTTACTTTATGTACCGTGGCGTAAT                                                                                                                                <  3:966832/150‑1
                                          CTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATT                                                                                                                                                                 <  1:16166/109‑1
                                          CTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATT                                                                                                                                                                 >  2:16166/1‑109
                                           TAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTA                                                                                                                                                                >  3:63200/1‑109
                                           TAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTA                                                                                                                                                                <  4:63200/109‑1
                                                   ACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTTACTTTATGTACCGTGGCGTAA                                                                                                                                 <  2:1135045/132‑1
                                                   ACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTTACTTTATGTACCGTGGCGTAA                                                                                                                                 >  1:1135045/1‑132
                                                                       ATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTTACTTTATGTACCGTGGCGTAATGTTTTATGAA                                                                                                                      >  3:1004407/1‑123
                                                                       ATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTTACTTTATGTACCGTGGCGTAATGTTTTATGAA                                                                                                                      <  4:1004407/123‑1
                                                                               TTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGC                                                                                                                                                             <  1:600700/76‑1
                                                                               TTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGC                                                                                                                                                             >  2:600700/1‑76
                                                                               TTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTAC                                                                                                                                                               >  1:1477921/1‑74
                                                                               TTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTAC                                                                                                                                                               <  2:1477921/74‑1
                                                                                  AAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTTACTTTATGTACCGTGGCGTAATGTTTTATGAATGAAGTATTAAGTTGCAATTGTCTGGTCAATATTAACG                                                                                <  1:1911/150‑1
                                                                                                GCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTTACTTTATGTACCGTG                                                                                                                                       <  2:1259020/81‑1
                                                                                                GCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTTACTTTATGTACCGTG                                                                                                                                       >  1:1259020/1‑81
                                                                                                         ATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTTACTTTATGTACCGTGGCGTAATGTTTTATGAATGAAGTATTAAGTTGCAATTGTCTGGTCAATATTAACGGTGCGTTTTTGCTGACTTTAAGG                                                         >  1:1461595/1‑150
                                                                                                           ATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTATGCCACGGTTACTTTATGTACCGTGGCGTAATGTTTTATGAATGAAGTATTAAGTTGCAATTGTCTGGTCAATATTAACGGTGCGTTTTTGCTGACTTTAAGG                                                         >  4:417491/1‑148
                                                                                                           ATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTATGCCACGGTTACTTTATGTACCGTGGCGTAATGTTTTATGAATGAAGTATTAAGTTGCAATTGTCTGGTCAATATTAACGGTGCGTTTTTGCTGACTTTAAGG                                                         <  3:417491/148‑1
                                                                                                                 CAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTTACTTTATGTACCGTGGCGTAATGTTTTATGAATGAAGTATTAAGTTGCAATTGTCTGGTCAATATTAACGGTGCGTTTTTGCTGACTTTAAGGCAGTATGT                                                 >  2:382998/1‑150
                                                                                                                          GCAACTTAATACTTCATTCATAAAACATTACGCCACGGTTACTTTATGTACCGTGGCGTAATGTTTTATGAATGAAGTATTAAGTTGCAATTGTCTGGTCAATATTAACGGTGCGTTTTTGCTGACTTTAAGGCAGTATGTAACGATGCA                                        <  2:1461595/150‑1
                                                                                                                                TAATACTTCATTCATAAAACATTACGCCACGGTTACTTTATGTACCGTGGCGTAATGTTTTATGAATGAAGTATTAAGTTGCAATTGTCTGGTCAATATTAACGGTGCGTTTTTGCTGACTTTAAGGCAGTATGTAACGATGCAACAGTA                                  <  1:382998/150‑1
                                                                                                                                                   CATTACGCCACGGTTACTTTATGTACCGTGGCGTAATGTTTTATGAATGAAGTATTAAGTTGCAATTGTCTGGTCAATATTAACGGTGCGTTTTTGCTGACTTTAAGGCAGTATGTAACGATGCAACAGTAAGTTAGCCTTAGTGAATGT               >  1:448955/1‑150
                                                                                                                                                         GCCACGGTTACTTTATGTACCGTGGCGTAATGTTTTATGAATGAAGTATTAAGTTGCAATTGTCTGGTCAATATTAACGGTGCGTTTTTGCTGACTTTAAGGCAGTATGTAACGATGCAACAGTAAGTTAGCCTTAGTGAATGTGGGCTT         >  2:859572/1‑150
                                                                                                                                                          CCACGGTTACTTTATGTACCGTGGCGTAATGTTTTATGAATGAAGTATTAAGTTGCAATTGTCTGGTCAATATTAACGGTGCGTTTTTGCTGACTTTAAGGCAGTATGTAACGATGCAACAGTAAGTTAGCCTTAGTGAATGTGGGCTTT        <  2:455512/150‑1
                                                                                                                                                          CCACGGTTACTTTATGTACCGTGGCGTAATGTTTTATGAATGAAGTATTAAGTTGCAATTGTCTGGTCAATATTAACGGTGCGTTTTTGCTGACTTTAAGGCAGTATGTAACGATGCAACAGTAAGTTA                             >  1:643754/1‑129
                                                                                                                                                          CCACGGTTACTTTATGTACCGTGGCGTAATGTTTTATGAATGAAGTATTAAGTTGCAATTGTCTGGTCAATATTAACGGTGCGTTTTTGCTGACTTTAAGGCAGTATGTAACGATGCAACAGTAAGTTA                             <  2:643754/129‑1
                                                                                                                                                           CACGGTTACTTTATGTACCGTGGCGTAATGTTTTATGAATGAAGTATTAAGTTGCAATTGTCTGGTCAATATTAACGGTGCGTTTTTGCTGACTTTAAGGCAGTATGTAACGATGCAACAGTAAGTTAGCCTTAGTGAATGTGGGCTTTG       <  3:839001/150‑1
                                                                                                                                                           CACGGTTACTTTATGTACCGTGGCGTAATGTTTTATGAATGAAGTATTAAGTTGCAATTGTCTGGTCAATATTAACGGTGCGTTTTTGCTGACTTTAAGGCAGTATGTAACGATGCAACAGTAAGTTAGCCTTAGTGAATGTGGGCTTTG       >  4:839001/1‑150
                                                                                                                                                           CACGGTTACTTTATGTACCGTGGCGTAATGTTTTATGAATGAAGTATTAAGTTGCAATTGTCTGGTCAATATTAACGGTGCGTTTTTGCTGACTTTAAGGCAGTATGTAACGATGCAACAGTAAGTTAGCCTTAG                      <  1:1173802/135‑1
                                                                                                                                                           CACGGTTACTTTATGTACCGTGGCGTAATGTTTTATGAATGAAGTATTAAGTTGCAATTGTCTGGTCAATATTAACGGTGCGTTTTTGCTGACTTTAAGGCAGTATGTAACGATGCAACAGTAAGTTAGCCTTAG                      >  2:1173802/1‑135
                                                                                                                                                           CACGGTTACTTTATGTACCGTGGCGTAACGTTTTATGAATGAAGTATTAAGTTGCAATTGTCTGGTCAATATTAACGGTGCGTTTTTGCTGACTTTAAGGCAGTATGTAACGATGCAACAGTAAGTTAGCCTTAGTGAATGTGGGCTTTG       >  1:1664194/1‑150
                                                                                                                                                              GGTTACTTTATGTACCGTGGCGTAATGTTTTATGAATGAAGTATTAAGTTGCAATTGTCTGGTCAATATTAACGGTGCGTTTTTGCTGACTTTAAGGCAGTATGTAACGATGCAACAGTAAGTTAGCCTTAGTGAATGTGGGCTTTGTCA    <  1:404560/150‑1
                                                                                                                                                               GTTACTTTATGTACCGTGGCGTAATGTTTTATGAATGAAGTATTAAGTTGCAATTGTCTGGTCAATATTAACGGTGCGTTTTTGCTGACTTTAAGGCAGTATGTAACGATGCAACAGTAAGTTAGCCTTAGTGAATGTGGGCTTTGTCAC   <  1:264372/150‑1
                                                                                                                                                               GTTACTTTATGTACCGTGGCGTAATGTTTTATGAATGAAGTATTAAGTTGCAATTGTCTGGTCAATATTAACGGTGCGTTTTTGCTGACTTTAAGGCAGTATGTAACGATGCAACAGTAAGTTAGCCTTAGTGAATGTGGGCTTTGTCAC   <  2:1395346/150‑1
                                                                                                                                                               GTTACTTTATGTACCGTGGCGTAATGTTTTATGAATGAAGTATTAAGTTGCAATTGTCTGGTCAATATTAACGGTGCGTTTTTGCTGACTTTAAGGCAGTATGTAACGATGCAACAGTAAGTTAGCCTTAGTGAATGTGGGCTTTGTCAC   <  1:715859/150‑1
                                                                                                                                                                TTACTTTATGTACCGTGGCGTAATGTTTTATGAATGAAGTATTAAGTTGCAATTGTCTGGTCAATATTAACGGTGCGTTTTTGCTGACTTTAAGGCAGTATGTAACGATGCAACAGTAAGTTAGCCTTAGTGAATGTGGGCTTTGTCACG  <  1:1682104/150‑1
                                                                                                                                                                                                                                                                                                                      
AGTATAAGACCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_KO_pgi/2642940‑2643100
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cattacgccacggtTACTTTATGTACCGTGGCGTAATGTTTTATGAATGAAGTATTAAGTTGCAATTGTCTGGTCAATATTAACGGTGCGTTTTTGCTGACTTTAAGGCAGTATGTAACGATGCAACAGTAAGTTAGCCTTAGTGAATGTGGGCTTTGTCACG  <  NC_000913_3_KO_pgi/2643110‑2642962

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.