New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_KO_pgi = 2643100126 (0.830)17 (0.120) 16/246 14.3 13.1% intergenic (‑256/+29) rodZ/rlmN MreB assembly cytoskeletal protein/dual specificity 23S rRNA m(2)A2503, tRNA m(2)A37 methyltransferase, SAM‑dependent
?NC_000913_3_KO_pgi = 2643110 112 (0.820)intergenic (‑266/+19) rodZ/rlmN MreB assembly cytoskeletal protein/dual specificity 23S rRNA m(2)A2503, tRNA m(2)A37 methyltransferase, SAM‑dependent
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

Only 100 of 131 total aligned reads displayed.

TCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAGGGTACGTTTCGTACGGTCGATAACATCGCCCGCCAACTGACCACAGGCAGCATCGATATCATCACCACGAGTTTTACG  >  NC_000913_3_KO_pgi/2642961‑2643254
                                                                                                                                                      |                                                                                                                                                
tCGTGACAAAGCCCACCTTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTACATAAAGTa                                                                                                                                                  >  3:828044/1‑150 (MQ=255)
tCGTGACAAAGCCCACCTTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTACATAAAGTa                                                                                                                                                  >  3:827971/1‑150 (MQ=255)
tCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTACATAAAGTa                                                                                                                                                  >  2:774440/1‑150 (MQ=255)
 cGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTACATAAAGTaa                                                                                                                                                 >  1:1682104/1‑150 (MQ=255)
  gTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTACATAAAGTAAc                                                                                                                                                >  2:1395346/1‑150 (MQ=255)
  gTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTACATAAAGTAAc                                                                                                                                                >  1:715859/1‑150 (MQ=255)
  gTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTACATAAAGTAAc                                                                                                                                                >  1:264372/1‑150 (MQ=255)
   tGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTACATAAAGTAAcc                                                                                                                                               >  1:404560/1‑150 (MQ=255)
      cAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTACATAAAGTAACCGTg                                                                                                                                            <  4:839001/150‑1 (MQ=255)
       aaaGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTACATAAAGTAACCGTgg                                                                                                                                           >  2:455512/1‑150 (MQ=255)
        aaGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTACATAAAGTAACCGTGGc                                                                                                                                          <  2:859572/150‑1 (MQ=255)
             cacaTTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTTACTTTATGTACCGTGGCGTAAt                                                                                                                                     <  3:966832/150‑1 (MQ=38)
              acaTTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTACATAAAGTAACCGTGGCGTAATg                                                                                                                                    <  1:448955/150‑1 (MQ=255)
               caTTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTACATAAAGTAACCGTGGCGTAATgg                                                                                                                                   <  2:359403/150‑1 (MQ=255)
                  tCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTACATAAAGTAACCGTGGCGTAATGGCTa                                                                                                                                <  3:59929/150‑1 (MQ=255)
                   cACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTACATAAAGTAACCGTGGCGTAATGGCTAt                                                                                                                               <  3:399784/150‑1 (MQ=255)
                    aCTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTACATAAAGTAACCGTGGCGTAATGGCTATc                                                                                                                              <  2:576453/150‑1 (MQ=255)
                     cTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTACATAAAGTAACCGTg                                                                                                                                            <  2:1173802/135‑1 (MQ=255)
                     cTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTACATAAAGTAACCGTg                                                                                                                                            >  1:1173802/1‑135 (MQ=255)
                            tAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTACATAAAGTAACCGTgg                                                                                                                                           <  1:643754/129‑1 (MQ=255)
                            tAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTACATAAAGTAACCGTgg                                                                                                                                           >  2:643754/1‑129 (MQ=255)
                                 tACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAAt                                                                                                                 <  4:78290/150‑1 (MQ=255)
                                   cTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGt                                                                                                               <  3:868655/150‑1 (MQ=255)
                                   cTGTTGCATCGTGACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACGTCATTCATAAAACATTACGCCACGGTACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGt                                                                                                               <  2:473179/150‑1 (MQ=255)
                                       tGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGAt                                                                                                           <  4:339251/150‑1 (MQ=255)
                                       tGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGAt                                                                                                           >  2:1213233/1‑150 (MQ=255)
                                        gCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATg                                                                                                          >  1:530285/1‑150 (MQ=255)
                                               tACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCAc                                                                                                   <  2:439572/150‑1 (MQ=255)
                                                 cATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCAccc                                                                                                 >  3:567526/1‑150 (MQ=255)
                                                  aTACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTACATAAAGTAACCGTGGCGTAATGGCTATCAGCCCGCTTTAATGTCGATGGCTTCAcccc                                                                                                >  2:1419552/1‑149 (MQ=255)
                                                  aTACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGt                                                                                                               >  2:236242/1‑135 (MQ=255)
                                                  aTACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGt                                                                                                               <  1:236242/135‑1 (MQ=255)
                                                  aTACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCt                                                                                                <  2:171173/150‑1 (MQ=255)
                                                  aTACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCt                                                                                                <  1:5862/150‑1 (MQ=255)
                                                   tACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTg                                                                                               <  2:530285/150‑1 (MQ=255)
                                                    aCTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCt                                                                                                >  1:1094519/1‑148 (MQ=255)
                                                    aCTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGc                                                                                              <  2:649947/150‑1 (MQ=255)
                                                    aCTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGc                                                                                              >  2:1037051/1‑150 (MQ=255)
                                                     cTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCa                                                                                             >  2:1021316/1‑150 (MQ=255)
                                                        ccTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATcc                                                                                          >  1:1146037/1‑150 (MQ=255)
                                                          ttAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGt                                                                                        >  2:90840/1‑150 (MQ=255)
                                                          ttAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGt                                                                                        >  2:606265/1‑150 (MQ=255)
                                                              aGTCAGCGAAAACTCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGTTTCACCCTGCATCCGTTTAc                                                                                    <  2:1462538/150‑1 (MQ=255)
                                                                  aGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAg                                                                                >  1:413745/1‑150 (MQ=255)
                                                                   gCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAgg                                                                               >  1:495988/1‑150 (MQ=255)
                                                                   gCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAg                                                                                >  3:773206/1‑149 (MQ=255)
                                                                   gCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAg                                                                                <  4:773206/149‑1 (MQ=255)
                                                                    cAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAggg                                                                              >  2:470043/1‑150 (MQ=255)
                                                                     aaaaaCGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAGGGt                                                                             >  1:912612/1‑150 (MQ=255)
                                                                       aaaCGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAGGGTAc                                                                           >  2:1290716/1‑150 (MQ=255)
                                                                             aCCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAGGGTACGTTTCg                                                                     >  3:936289/1‑150 (MQ=255)
                                                                                  tAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAGGGTACGTTTCGTACgg                                                                <  2:984207/150‑1 (MQ=255)
                                                                                   aaTATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAGGGTACGTTTCGTACGGt                                                               <  2:1146037/150‑1 (MQ=255)
                                                                                     tatTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAg                                                                                >  4:673744/1‑131 (MQ=255)
                                                                                      atTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCt                                                                                                                      <  2:1082902/92‑1 (MQ=255)
                                                                                      atTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCt                                                                                                                      >  1:1082902/1‑92 (MQ=255)
                                                                                      atTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAGGGTACGTTTCGTACGGTCGa                                                            <  2:549553/150‑1 (MQ=255)
                                                                                         gACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAGGGTACGTTTCGTACGGTCGATaa                                                         >  1:1537102/1‑150 (MQ=255)
                                                                                          aCCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAGGGTACGTTTCGTACGGTCGATAAc                                                        >  2:98017/1‑150 (MQ=255)
                                                                                             aGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCt                                                                                                                      <  4:1002204/85‑1 (MQ=255)
                                                                                             aGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAGGGTACGTTTCGTACGGTCGATAACATc                                                     <  1:645696/150‑1 (MQ=255)
                                                                                             aGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAGGGTACGTTTCGTACGGTCGATAACATc                                                     >  3:302983/1‑150 (MQ=255)
                                                                                             aGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAGGGTACGTTTCGTACGGTCGATAACATc                                                     >  1:16997/1‑150 (MQ=255)
                                                                                                     gCAACTTAATACTTCATTCATAAAACATTACGCCACGGTACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAGGGTACGTTTCGTACGGTCGATAACATCGCCCGCCa                                             >  2:1134343/1‑150 (MQ=255)
                                                                                                      cAACTTAATACTTCATTCATAAAACATTACGCCACGGTACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAGGGTACGTTTCGTACGGTCGATAACATCGCCCGCCaa                                            >  2:1571291/1‑150 (MQ=255)
                                                                                                       aaCTTAATACTTCATTCATAAAACATTACGCCACGGTACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAGGGTACGTTTCGTACGGTCGATAACATCGCCCGCCAAc                                           >  1:917963/1‑150 (MQ=255)
                                                                                                        aCTTAATACTTCATTCATAAAACATTACGCCACGGTACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAGGGTACGTTTCGTACGGTCGATAACATCGCCCGCCAACt                                          <  3:789997/150‑1 (MQ=255)
                                                                                                          ttAATACTTCATTCATAAAACATTACGCCACGGTACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAGGGTACGTTTCGTACGGTCGATAACATCGCCCGCCAACTGa                                        <  1:1290716/150‑1 (MQ=255)
                                                                                                             aTACTTCATTCATAAAACATTACGCCACGGTACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAGGGTACGTTTCGTACGGTCGATAACATCGCCCGCCAACTGACca                                     <  1:1395346/150‑1 (MQ=255)
                                                                                                              tACTTCATTCATAAAACATTACGCCACGGTACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAGGGTACGTTTCGTACGGTCGATAACATCGCCCGCCAACTGACcac                                    <  2:1682104/150‑1 (MQ=255)
                                                                                                                 ttcattcaTAAAACATTACGCCACGGTACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAGGGTACGTTTCGTACGGt                                                               <  1:243990/120‑1 (MQ=255)
                                                                                                                 ttcattcaTAAAACATTACGCCACGGTACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAGGGTACGTTTCGTACGGt                                                               >  2:243990/1‑120 (MQ=255)
                                                                                                                 ttcattcaTAAAACATTACGCCACGGTACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAGGGTACGTTTCGTACGGTCGATAACATCGCCCGCCAACTGACCACAgg                                 >  3:127584/1‑150 (MQ=255)
                                                                                                                  tcattcatAAAACATTACGCCACGGTACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAGGGTACGTTTCGTAc                                                                  <  3:739216/116‑1 (MQ=255)
                                                                                                                    attcatAAAACATTACGCCACGGTACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAGGGTACGTTTCGTACGGTCGATAACATCGCCCGCCAACTGACCACAGgcag                              >  4:62090/1‑150 (MQ=255)
                                                                                                                        atAAAACATTACGCCACGGTACATAAAGTACCCGTGGCGTCATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAGGGTACGTTTCGTACGGTCGATAACATCGCCCGCCAACTGACCACAGGCAGCATc                          <  1:936268/150‑1 (MQ=255)
                                                                                                                         tAAAACATTACGCCACGGTACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACg                                                                                   >  4:778033/1‑92 (MQ=255)
                                                                                                                         tAAAACATTACGCCACGGTACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACg                                                                                   <  3:778033/92‑1 (MQ=255)
                                                                                                                         tAAAACATTACGCCACGGTACATAAAGTAACCGCGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGCTTACGCAGGGTACGTTTCGTACGGTCGATAACATCGCCCGCCAACTGACCACAGGCAGCATCg                         >  3:625741/1‑150 (MQ=255)
                                                                                                                          aaaaCATTACGCCACGGTACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAGGGTACGTTTCGTACGGTCGATAACATCGCCCGCCAACt                                          >  4:143929/1‑132 (MQ=255)
                                                                                                                          aaaaCATTACGCCACGGTACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAGGGTACGTTTCGTACGGTCGATAACATCGCCCGCCAACt                                          <  3:143929/132‑1 (MQ=255)
                                                                                                                          aaaaCATTACGCCACGGTACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAGGGTACGTTTCGTACGGTCGATAACATCGCCCGCCAACTGACCACAGGCAGCATCGa                        <  1:1037051/150‑1 (MQ=255)
                                                                                                                           aaaCATTACGCCACGGTACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAGGGTACGTTTCGTACGGTCGATAACATCGCCCGCCAACTGACCACAg                                  >  3:84204/1‑139 (MQ=255)
                                                                                                                           aaaCATTACGCCACGGTACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAGGGTACGTTTCGTACGGTCGATAACATCGCCCGCCAACTGACCACAg                                  <  4:84204/139‑1 (MQ=255)
                                                                                                                            aaCATTACGCCACGGTACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAGGGTACGTTTCGTACGGTCGATAACATCGCCCGCCAACTGACCACAGGCAGCATCGata                      >  2:659332/1‑150 (MQ=255)
                                                                                                                               aTTACGCCACGGTACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAGGGTACGTTTCGTACGGTCGATAACATCGCCCGCCAACTGACCACAGGCAGCATCGATatca                   >  2:129086/1‑150 (MQ=255)
                                                                                                                                ttACGCCACGGTACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAGGGTACGTTTCGTACGGTCGATAACATCGCCCGCCAACTGACCACAGGCAGCATCGATatcat                  <  2:1473774/150‑1 (MQ=255)
                                                                                                                                   cGCCACGGTACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAGGGTACGTTTCGTACGGTCGATAACATCGCCCGCCAACTGACCACAGGCAGCATCGATATCATcac               <  2:482160/150‑1 (MQ=255)
                                                                                                                                    gCCACGGTACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAGGGTACGTTTCGTACGGTCGATAACATCGCCCGCCAACTGACCACAGGCAGCATCGATATCATcacc              >  1:1163727/1‑150 (MQ=255)
                                                                                                                                       aCGGTACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAGGGTACGTTTCGTACGGTCGATAACATCGCCCGCCAACTGACCACAGGCAGCATCGATATCATCACCACg           >  4:726664/1‑150 (MQ=255)
                                                                                                                                          gTACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAGGGTACGTTTCGTACGGTCGATAACATCGCCCGCCAACTGACCACAGGCAGCATCGATATCATCACCACGAGt        <  2:413745/150‑1 (MQ=255)
                                                                                                                                          gTACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAGGGTACGTTTCGTACGGTCGATAACATCGCCCGCCAACTGACCACAGGCAGCATCGATATCATCACCACGAGt        <  1:1021316/150‑1 (MQ=255)
                                                                                                                                             aCATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAGGGTACGTTTCGTACGGTCGATAACATCGCCCGCCAACTGACCACAGGCAGCATCGATATCATCACCACGAGttt      >  2:1393122/1‑150 (MQ=255)
                                                                                                                                              cATAAAGTAACCGTGGCGTAACGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAGGGTACGTTTCGTACGGTCGATAACATCGCCCGCCAACTGACCACAGGCAGCATCGATATCATCACCACGAGtttt     <  2:206340/150‑1 (MQ=255)
                                                                                                                                                tAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCCGCATCCGTTTACGCAGGGTACGTTTCGTACGGTCGATAACATCGCCCGCCAACTGACCACAGGCAGCATCGATATCATCACCACGAGTTTTAc   <  1:29586/150‑1 (MQ=255)
                                                                                                                                                 aaaGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAGGGTACGTTTCGTACGGTCGATAACATcg                                                    >  2:961976/1‑100 (MQ=255)
                                                                                                                                                 aaaGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAGGGTACGTTTCGTACGGTCGATAACATcg                                                    <  1:961976/100‑1 (MQ=255)
                                                                                                                                                 aaaGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAGGGTACGTTTCGTACGGTCGATAACATCGCCCGCCAACTGACCACAGGCAGCATCGATATCATCACCACGAGTTTTACg  >  4:968110/1‑150 (MQ=255)
                                                                                                                                                    gTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAgg                                                                               <  2:198056/70‑1 (MQ=255)
                                                                                                                                                    gTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAgg                                                                               >  1:198056/1‑70 (MQ=255)
                                                                                                                                                      |                                                                                                                                                
TCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAGGGTACGTTTCGTACGGTCGATAACATCGCCCGCCAACTGACCACAGGCAGCATCGATATCATCACCACGAGTTTTACG  >  NC_000913_3_KO_pgi/2642961‑2643254

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.