Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913_3_KO_pgi 3,397,657 Δ9 bp coding (131‑139/1470 nt) rng ← ribonuclease G

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913_3_KO_pgi 3397657 3397665 9 58 [0] [0] 58 rng ribonuclease G

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_KO_pgi = 33976560 (0.000)56 (1.030) 48/240 0.2 100% coding (140/1470 nt) rng ribonuclease G
?NC_000913_3_KO_pgi 3397666 = 0 (0.000)coding (130/1470 nt) rng ribonuclease G

CAGTTCCGAGATGTCGCGCACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGTTTACTAACAATTCAGCCGTCATGTTTATCC  >  NC_000913_3_KO_pgi/3397508‑3397803
                                                                                                                                                    |                                                                                                                                                   
cAGTTCCGAGATGTCGCGCACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACc                                                                                                                                                    >  2:662118‑M1/1‑149 (MQ=255)
      cGAGATGTCGCGCACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgta                                                                                                                                              >  4:393828‑M1/1‑143 (MQ=255)
           tGTCGCGCACCGTGAATTGCTTTTTTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatat                                                                                                                                         <  2:342830‑M1/150‑13 (MQ=255)
                 gcACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgccta                                                                                                                                   <  1:412100‑M1/150‑19 (MQ=255)
                         aaTTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccg                                                                                                                           >  1:434278‑M1/1‑124 (MQ=255)
                               ttttGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAATAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgc                                                                                                                     >  3:349036‑M1/1‑118 (MQ=255)
                               ttttGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgc                                                                                                                     <  1:467765‑M1/150‑33 (MQ=255)
                                      cttcACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcac                                                                                                              >  1:664797‑M1/1‑111 (MQ=255)
                                            ccGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcccgttcaa                                                                                                        >  1:686802‑M1/1‑105 (MQ=255)
                                                 acacaTTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatga                                                                                                   <  4:271913‑M1/150‑51 (MQ=255)
                                                  cacaTTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaa                                                                                                  >  1:85727‑M1/1‑99 (MQ=255)
                                                      ttCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttc                                                                                              >  4:223324‑M1/1‑95 (MQ=255)
                                                      ttCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgtt                                                                                                           >  1:233975‑M1/1‑95 (MQ=255)
                                                      ttCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgtt                                                                                                           <  2:233975‑M1/137‑43 (MQ=255)
                                                        cGGTGTGCGGCATGATGTGGGATGGATGAAGAGACGCGGCGTTATCCAGCCCAATATATACAAAAGGCGCCTGCATACGCGGAAGTAAACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcct                                                                                            <  2:606854‑M1/150‑58 (MQ=255)
                                                        cGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcct                                                                                            <  3:393828‑M1/150‑58 (MQ=255)
                                                               cGGCATGATGTCGGATGCATGAAGAAACGCGTCTTTATCCAGCCCAATAGCTACAAAAGCCGCCTGCATACCGGGAAGGACACGACtcttgtagatattgcctactactccgcgtcgcgcctcacgttcaatatgagtttcccgcagaat                                                                                     >  2:33598‑M1/1‑86 (MQ=255)
                                                                ggCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaata                                                                                    >  4:125300‑M1/1‑85 (MQ=255)
                                                                  cATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaat                                                                                                       >  1:661569‑M1/1‑83 (MQ=255)
                                                                  cATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaat                                                                                                       <  2:661569‑M1/129‑47 (MQ=255)
                                                                    tgatgTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgccccacgttcaatatgactttcccgccgaataccgc                                                                                >  2:657650‑M1/1‑81 (MQ=255)
                                                                     gatgTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgcc                                                                               <  2:434278‑M1/150‑71 (MQ=255)
                                                                       tgTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccat                                                                             <  3:125300‑M1/150‑73 (MQ=255)
                                                                                 aTGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAACAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacnttcaatatgaatttnctgcagaataccgccatcaatatacgc                                                                   <  1:657650‑M1/150‑83 (MQ=255)
                                                                                 aTGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgc                                                                   <  1:662118‑M1/150‑83 (MQ=255)
                                                                                 aTGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaata                                                                                                      >  4:152418‑M1/1‑68 (MQ=255)
                                                                                 aTGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaata                                                                                                      <  3:152418‑M1/115‑48 (MQ=255)
                                                                                   gaagaaACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaata                                                                        <  2:238754‑M1/143‑78 (MQ=255)
                                                                                   gaagaaACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaata                                                                        >  1:238754‑M1/1‑66 (MQ=255)
                                                                                   gaagaaACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatat                                                                                                                                         <  1:9935‑M1/78‑13 (MQ=255)
                                                                                   gaagaaACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatat                                                                                                                                         >  2:9935‑M1/1‑66 (MQ=255)
                                                                                    aagaaACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatata                                                                      <  1:498742‑M1/144‑80 (MQ=255)
                                                                                    aagaaACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatata                                                                      >  2:498742‑M1/1‑65 (MQ=255)
                                                                                       aaaCGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactc                                                              <  2:346319‑M1/149‑88 (MQ=255)
                                                                                       aaaCGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactc                                                              >  1:346319‑M1/1‑62 (MQ=255)
                                                                                           gcgGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgcca                                                                              >  2:419355‑M1/1‑58 (MQ=255)
                                                                                           gcgGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgcca                                                                              <  1:419355‑M1/129‑72 (MQ=255)
                                                                                                    tCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttccgaaggc                                                >  2:467276‑M1/1‑49 (MQ=255)
                                                                                                      cAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttccgaaggcgt                                              >  2:621534‑M1/1‑47 (MQ=255)
                                                                                                       aGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttcc                                                      <  1:678886‑M1/141‑96 (MQ=255)
                                                                                                       aGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttcc                                                      >  2:678886‑M1/1‑46 (MQ=255)
                                                                                                               atCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttccgaaggcgttacgtttac                                     <  1:668076‑M1/150‑113 (MQ=255)
                                                                                                                 cTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaatactgccatcaatatacgccactcgcgtttccgaaggcgttacgtttacta                                   >  2:691670‑M1/1‑36 (MQ=255)
                                                                                                                  tACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttccgaaggcgttacgtttactaa                                  >  2:359058‑M1/1‑35 (MQ=255)
                                                                                                                   aCAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttccgaaggcgttacgtttactaac                                 >  3:144340‑M1/1‑34 (MQ=255)
                                                                                                                   aCAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttccgaaggcgttacgtttactaac                                 >  2:296952‑M1/1‑34 (MQ=255)
                                                                                                                   aCAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccat                                                                             <  2:212103‑M1/106‑73 (MQ=255)
                                                                                                                   aCAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccat                                                                             >  1:212103‑M1/1‑34 (MQ=255)
                                                                                                                    cAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaat                                                                                                 >  2:673266‑M1/1‑33 (MQ=255)
                                                                                                                    cAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaat                                                                                                 <  1:673266‑M1/85‑53 (MQ=255)
                                                                                                                         gccgccTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttccgaaggcgttacgtttactaacaattca                           <  2:85727‑M1/150‑123 (MQ=255)
                                                                                                                          ccgccTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttccgaaggcgttacgtttactaacaattcag                          >  2:542753‑M1/1‑27 (MQ=255)
                                                                                                                          ccgccTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttccgaaggcgttacgtttactaacaattcag                          <  1:621534‑M1/150‑124 (MQ=255)
                                                                                                                                 cATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttccgaaggcgttacgtttactaacaattcagccgtcat                   <  1:33598‑M1/150‑131 (MQ=255)
                                                                                                                                   tACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttccgaaggcgttacgtttactaacaattcagccgtcatgt                 <  2:664797‑M1/150‑133 (MQ=255)
                                                                                                                                     cccGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagactaccgccatcaatatacgcgacacgggtttcaaaagctgttacgattactaaaaaatcagccataatgttt               >  3:403530‑M1/1‑16 (MQ=255)
                                                                                                                                              acacGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttccgaaggcgttacgtttactaacaattcagccgtcatgtttatccctttt      >  1:7127‑M1/1‑7 (MQ=255)
                                                                                                                                                  gACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttccgaaggcgttacgtttactaacaattcagccgtcatgtttatcccttttctca  >  4:372157‑M1/1‑3 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CAGTTCCGAGATGTCGCGCACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGTTTACTAACAATTCAGCCGTCATGTTTATCC  >  NC_000913_3_KO_pgi/3397508‑3397803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.