Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913_3_KO_pgi 3,397,657 Δ9 bp coding (131‑139/1470 nt) rng ← ribonuclease G

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913_3_KO_pgi 3397657 3397665 9 58 [0] [0] 58 rng ribonuclease G

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_KO_pgi = 33976560 (0.000)56 (1.030) 48/240 0.2 100% coding (140/1470 nt) rng ribonuclease G
?NC_000913_3_KO_pgi 3397666 = 0 (0.000)coding (130/1470 nt) rng ribonuclease G

GATGTCGCGCACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGTTTACTAACAATTCAGCCGTCATGTTTATCCCTTTTCTCA  >  NC_000913_3_KO_pgi/3397517‑3397812
                                                                                                                                                     |                                                                                                                                                  
cagttccgagatgtcgcgcaccgtgaattgcttttgttcttcacccgccacacattcggtgtgcggcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacc                                                                                                                                                    >  2:662118‑M2/150‑150 (MQ=255)
      cgagatgtcgcgcaccgtgaattgcttttgttcttcacccgccacacattcggtgtgcggcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTa                                                                                                                                              >  4:393828‑M2/144‑150 (MQ=255)
           tgtcgcgcaccgtgaattgctttttttcttcacccgccacacattcggtgtgcggcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGatat                                                                                                                                         <  2:342830‑M2/12‑1 (MQ=255)
                 gcaccgtgaattgcttttgttcttcacccgccacacattcggtgtgcggcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCcta                                                                                                                                   <  1:412100‑M2/18‑1 (MQ=255)
                         aattgcttttgttcttcacccgccacacattcggtgtgcggcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCcg                                                                                                                           >  1:434278‑M2/125‑150 (MQ=255)
                               ttttgttcttcacccgccacacattcggtgtgcggcatgatgtcggatgcatgaataaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTcgc                                                                                                                     >  3:349036‑M2/119‑150 (MQ=255)
                               ttttgttcttcacccgccacacattcggtgtgcggcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTcgc                                                                                                                     <  1:467765‑M2/32‑1 (MQ=255)
                                      cttcacccgccacacattcggtgtgcggcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCAc                                                                                                              >  1:664797‑M2/112‑150 (MQ=255)
                                            ccgccacacattcggtgtgcggcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCCCGTTCaa                                                                                                        >  1:686802‑M2/106‑150 (MQ=255)
                                                 acacattcggtgtgcggcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGa                                                                                                   <  4:271913‑M2/50‑1 (MQ=255)
                                                  cacattcggtgtgcggcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGaa                                                                                                  >  1:85727‑M2/100‑150 (MQ=255)
                                                      ttcggtgtgcggcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGtt                                                                                                           >  1:233975‑M2/96‑137 (MQ=255)
                                                      ttcggtgtgcggcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGtt                                                                                                           <  2:233975‑M2/42‑1 (MQ=255)
                                                      ttcggtgtgcggcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTc                                                                                              >  4:223324‑M2/96‑150 (MQ=255)
                                                        cggtgtgcggcatgatgtgggatggatgaagagacgcggcgttatccagcccaatatatacaaaaggcgcctgcatacgcggaagtaaacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCt                                                                                            <  2:606854‑M2/57‑1 (MQ=255)
                                                        cggtgtgcggcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCt                                                                                            <  3:393828‑M2/57‑1 (MQ=255)
                                                               cggcatgatgtcggatgcatgaagaaacgcgtctttatccagcccaatagctacaaaagccgcctgcataccgggaaggacacgacTCTTGTAGATATTGCCTACTACTCCGCGTCGCGCCTCACGTTCAATATGAGTTTCCCGCAGAAt                                                                                     >  2:33598‑M2/87‑150 (MQ=255)
                                                                ggcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATa                                                                                    >  4:125300‑M2/86‑150 (MQ=255)
                                                                  catgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAat                                                                                                       >  1:661569‑M2/84‑129 (MQ=255)
                                                                  catgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAat                                                                                                       <  2:661569‑M2/46‑1 (MQ=255)
                                                                    tgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCCCACGTTCAATATGACTTTCCCGCCGAATACCGc                                                                                >  2:657650‑M2/82‑150 (MQ=255)
                                                                     gatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGcc                                                                               <  2:434278‑M2/70‑1 (MQ=255)
                                                                       tgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCAt                                                                             <  3:125300‑M2/72‑1 (MQ=255)
                                                                                 atgaagaaacgcggctttatccagcccaatatctacaacagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACNTTCAATATGAATTTNCTGCAGAATACCGCCATCAATATACGc                                                                   <  1:657650‑M2/82‑1 (MQ=255)
                                                                                 atgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAata                                                                                                      >  4:152418‑M2/69‑115 (MQ=255)
                                                                                 atgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAata                                                                                                      <  3:152418‑M2/47‑1 (MQ=255)
                                                                                 atgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGc                                                                   <  1:662118‑M2/82‑1 (MQ=255)
                                                                                   gaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGatat                                                                                                                                         <  1:9935‑M2/12‑1 (MQ=255)
                                                                                   gaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGatat                                                                                                                                         >  2:9935‑M2/67‑78 (MQ=255)
                                                                                   gaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAata                                                                        >  1:238754‑M2/67‑143 (MQ=255)
                                                                                   gaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAata                                                                        <  2:238754‑M2/77‑1 (MQ=255)
                                                                                    aagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAAtata                                                                      <  1:498742‑M2/79‑1 (MQ=255)
                                                                                    aagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAAtata                                                                      >  2:498742‑M2/66‑144 (MQ=255)
                                                                                       aaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTc                                                              >  1:346319‑M2/63‑149 (MQ=255)
                                                                                       aaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTc                                                              <  2:346319‑M2/87‑1 (MQ=255)
                                                                                           gcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCa                                                                              <  1:419355‑M2/71‑1 (MQ=255)
                                                                                           gcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCa                                                                              >  2:419355‑M2/59‑129 (MQ=255)
                                                                                                    tccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGc                                                >  2:467276‑M2/50‑150 (MQ=255)
                                                                                                      cagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGt                                              >  2:621534‑M2/48‑150 (MQ=255)
                                                                                                       agcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTcc                                                      >  2:678886‑M2/47‑141 (MQ=255)
                                                                                                       agcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTcc                                                      <  1:678886‑M2/95‑1 (MQ=255)
                                                                                                               atctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGTTTAc                                     <  1:668076‑M2/112‑1 (MQ=255)
                                                                                                                 ctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACTGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGTTTACTa                                   >  2:691670‑M2/37‑150 (MQ=255)
                                                                                                                  tacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGTTTACTaa                                  >  2:359058‑M2/36‑150 (MQ=255)
                                                                                                                   acaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCAt                                                                             >  1:212103‑M2/35‑106 (MQ=255)
                                                                                                                   acaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCAt                                                                             <  2:212103‑M2/72‑1 (MQ=255)
                                                                                                                   acaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGTTTACTAAc                                 >  3:144340‑M2/35‑150 (MQ=255)
                                                                                                                   acaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGTTTACTAAc                                 >  2:296952‑M2/35‑150 (MQ=255)
                                                                                                                    caaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAAt                                                                                                 >  2:673266‑M2/34‑85 (MQ=255)
                                                                                                                    caaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAAt                                                                                                 <  1:673266‑M2/52‑1 (MQ=255)
                                                                                                                         gccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGTTTACTAACAATTCa                           <  2:85727‑M2/122‑1 (MQ=255)
                                                                                                                          ccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGTTTACTAACAATTCAg                          >  2:542753‑M2/28‑150 (MQ=255)
                                                                                                                          ccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGTTTACTAACAATTCAg                          <  1:621534‑M2/123‑1 (MQ=255)
                                                                                                                                 catacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGTTTACTAACAATTCAGCCGTCAt                   <  1:33598‑M2/130‑1 (MQ=255)
                                                                                                                                   tacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGTTTACTAACAATTCAGCCGTCATGt                 <  2:664797‑M2/132‑1 (MQ=255)
                                                                                                                                     cccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGACTACCGCCATCAATATACGCGACACGGGTTTCAAAAGCTGTTACGATTACTAAAAAATCAGCCATAATGttt               >  3:403530‑M2/17‑150 (MQ=255)
                                                                                                                                              acacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGTTTACTAACAATTCAGCCGTCATGTTTATCCCtttt      >  1:7127‑M2/8‑150 (MQ=255)
                                                                                                                                                  gacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGTTTACTAACAATTCAGCCGTCATGTTTATCCCTTTTCTCa  >  4:372157‑M2/4‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
GATGTCGCGCACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGTTTACTAACAATTCAGCCGTCATGTTTATCCCTTTTCTCA  >  NC_000913_3_KO_pgi/3397517‑3397812

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.