Predicted mutation
evidence seq id position mutation annotation gene description
JC NC_000913_3_KO_pgi 4,233,757 +ATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTAC intergenic (‑524/‑583) lysC ← / → yjbE lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_KO_pgi = 42337570 (0.000)89 (0.980)
+ATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTAC
58/188 0.6 100% intergenic (‑524/‑583) lysC/yjbE lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein
?NC_000913_3_KO_pgi 4233758 = 0 (0.000)intergenic (‑525/‑582) lysC/yjbE lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein

Only 100 of 142 total aligned reads displayed.

GGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACC  >  NC_000913_3_KO_pgi/4233608‑4233892
                                                                                                                                                     |                                                                                                                                       
ggCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTa                                                                                                                                         <  2:524523/150‑1 (MQ=255)
ggCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTa                                                                                                                                         >  3:376825/1‑150 (MQ=255)
 gCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAa                                                                                                                                        <  1:565824‑M1/150‑2 (MQ=255)
   ggTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatg                                                                                                                                      <  3:884486‑M1/150‑4 (MQ=255)
           gtTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacat                                                                                                                              <  2:449815‑M1/150‑12 (MQ=255)
           gtTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacat                                                                                                                              <  1:589541‑M1/150‑12 (MQ=255)
           gtTCAGCTAATAAATGCTTCACTGCGCTAAGGGTGTACACGCTACATTAGTCTAACCGCACTAAATCCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAATTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacat                                                                                                                              <  1:570730‑M1/150‑12 (MQ=255)
           gtTCAGCTAATAAATGCTCCACTGCGCTAAGGGTTTACACTCAACCTTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGATTATTGCTAatgaaaaacat                                                                                                                              <  1:786997‑M1/150‑12 (MQ=255)
               aGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaat                                                                                                                          >  4:659399‑M1/1‑135 (MQ=255)
               aGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTACCAAGTCACAATTCTGACAATCAGAAGAGTATTGCTAatgaaaaacatcaat                                                                                                                          >  1:1008195‑M1/1‑135 (MQ=255)
                 cTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatcc                                                                                                                        >  1:128654‑M1/1‑133 (MQ=255)
                  taataaATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatcca                                                                                                                       >  4:191662‑M1/1‑132 (MQ=255)
                   aataaATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaa                                                                                                                      >  4:595899‑M1/1‑131 (MQ=255)
                    ataaATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaac                                                                                                                     >  3:450404‑M1/1‑130 (MQ=255)
                       aaTGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgca                                                                                                                  >  1:1281631‑M1/1‑127 (MQ=255)
                       aaTGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgca                                                                                                                  >  3:843721‑M1/1‑127 (MQ=255)
                          gCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagac                                                                                                               >  2:941713‑M1/1‑124 (MQ=255)
                            ttCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccg                                                                                                             >  1:688853‑M1/1‑122 (MQ=255)
                             tCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgc                                                                                                            >  2:1152550‑M1/1‑121 (MQ=255)
                              cACTGCGCTAAGCGTTTACACTCAACAGTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgct                                                                                                           <  1:129935‑M1/150‑31 (MQ=255)
                               aCTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCACTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctg                                                                                                          >  2:769534‑M1/1‑119 (MQ=255)
                               aCTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTCTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctg                                                                                                          >  2:324205‑M1/1‑119 (MQ=255)
                                 tGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcc                                                                                                        >  3:313665‑M1/1‑117 (MQ=255)
                                 tGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcc                                                                                                        >  3:668124‑M1/1‑117 (MQ=255)
                                   cgcTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctg                                                                                                      >  1:277758‑M1/1‑115 (MQ=255)
                                    gcTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctgg                                                                                                     <  2:205829‑M1/150‑37 (MQ=255)
                                    gcTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctgg                                                                                                     >  2:1277983‑M1/1‑114 (MQ=255)
                                     ctcagggTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAGCGTCACAATTCTCATGATCAGAAGAGTATTGCTCatgaaaaacatcaatccagcgcagaccgctgcctggc                                                                                                    <  2:1202954‑M1/147‑38 (MQ=255)
                                      tAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggca                                                                                                   >  3:543792‑M1/1‑112 (MQ=255)
                                      tAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggca                                                                                                   <  1:1177514‑M1/150‑39 (MQ=255)
                                             ttACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactg                                                                                            >  1:382058‑M1/1‑105 (MQ=255)
                                                 acTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccagcgcagaccgctgcctggcaggcactacggt                                                                                        >  1:927326‑M1/1‑101 (MQ=255)
                                                 acTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggt                                                                                        >  4:113140‑M1/1‑101 (MQ=255)
                                                 acTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctgg                                                                                                     <  2:971936‑M1/137‑37 (MQ=255)
                                                 acTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctgg                                                                                                     >  1:971936‑M1/1‑101 (MQ=255)
                                                   tCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgt                                                                                      >  3:687149‑M1/1‑99 (MQ=255)
                                                   tCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgt                                                                                      >  3:208064‑M1/1‑99 (MQ=255)
                                                   tCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgt                                                                                      >  4:688006‑M1/1‑99 (MQ=255)
                                                    cAACATTACGCTAACGGCACTACAACCATCCCATTTTTCTGTGACTGGCGCTACAATCTTACAAAGTCACAATTCTCCAGATCAGAAGAGTATTGCGAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgta                                                                                     <  2:853286‑M1/150‑53 (MQ=255)
                                                     aaCATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtag                                                                                    <  1:1096141‑M1/150‑54 (MQ=255)
                                                     aaCATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTACTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggggtag                                                                                    >  1:108645‑M1/1‑97 (MQ=255)
                                                       cATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggc                                                                                  >  4:445262‑M1/1‑95 (MQ=255)
                                                         ttACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctg                                                                                <  2:628214‑M1/150‑58 (MQ=255)
                                                           aCGCTAACGGCACTAAAACAATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctgga                                                                              <  2:1147037‑M1/150‑60 (MQ=255)
                                                             gCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagc                                                                            <  2:258564‑M1/150‑62 (MQ=255)
                                                              cTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagct                                                                           >  1:256567‑M1/1‑88 (MQ=255)
                                                              cTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagct                                                                           <  3:141215‑M1/150‑63 (MQ=255)
                                                               tAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgacaaccat                                                                                                                              <  2:636843‑M1/98‑12 (MQ=255)
                                                               tAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaccat                                                                                                                              >  1:636843‑M1/1‑87 (MQ=255)
                                                                   ggCACTAAAACCATCACATTTTTCTGTTACTTGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttc                                                                      >  2:917513‑M1/1‑83 (MQ=255)
                                                                      aCTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaa                                                                   <  2:592024‑M1/150‑71 (MQ=255)
                                                                      aCTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggggtaggctggagctgcttcgaa                                                                   >  1:483143‑M1/1‑80 (MQ=255)
                                                                        tAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagacggctgcctggcaggcactacggtgtaggctggagctgcttcgaagt                                                                 >  1:799768‑M1/1‑78 (MQ=255)
                                                                        tAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagt                                                                 >  3:28047‑M1/1‑78 (MQ=255)
                                                                          aaaCCATCACATTTTTCTTTGATTTGCTTTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttc                                                               >  4:409068‑M1/1‑76 (MQ=255)
                                                                          aaaCCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttc                                                               >  4:838297‑M1/1‑76 (MQ=255)
                                                                                tCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatac                                                         >  2:683078‑M1/1‑70 (MQ=255)
                                                                                tCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatac                                                         >  2:46477‑M1/1‑70 (MQ=255)
                                                                                    aTTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttc                                                     <  1:653619‑M1/150‑85 (MQ=255)
                                                                                        ttCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctaga                                                 <  1:421549‑M1/150‑89 (MQ=255)
                                                                                            gtgACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaat                                             <  3:722730‑M1/150‑93 (MQ=255)
                                                                                             tgACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaata                                            <  3:886828‑M1/150‑94 (MQ=255)
                                                                                              gACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaatag                                           <  3:727176‑M1/150‑95 (MQ=255)
                                                                                               aCTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaatagg                                          <  4:619940‑M1/150‑96 (MQ=255)
                                                                                               aCTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaatagg                                          <  1:510978‑M1/150‑96 (MQ=255)
                                                                                                 tGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaa                                        >  3:788506‑M1/1‑53 (MQ=255)
                                                                                                 tGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaa                                        >  3:788635‑M1/1‑53 (MQ=255)
                                                                                                   gcgcTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaact                                      >  3:285917‑M1/1‑51 (MQ=255)
                                                                                                    cgcTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaactt                                     <  1:1136376‑M1/150‑101 (MQ=255)
                                                                                                     gcTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttc                                    <  4:668124‑M1/150‑102 (MQ=255)
                                                                                                        aCAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaa                                 <  2:1284158‑M1/150‑105 (MQ=255)
                                                                                                        aCAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaa                                 <  1:20774‑M1/150‑105 (MQ=255)
                                                                                                             cTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgca                            <  3:688006‑M1/150‑110 (MQ=255)
                                                                                                                ccAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggggctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggt                         >  1:966901‑M1/1‑38 (MQ=255)
                                                                                                                ccAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggt                         <  2:739646‑M1/150‑113 (MQ=255)
                                                                                                                ccAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggt                         <  3:838297‑M1/150‑113 (MQ=255)
                                                                                                                ccAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctgg                                                                                                     >  4:134356‑M1/1‑38 (MQ=255)
                                                                                                                ccAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctgg                                                                                                     <  3:134356‑M1/74‑37 (MQ=255)
                                                                                                                  aaaGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacat                                                                                                                              <  3:317790‑M1/47‑12 (MQ=255)
                                                                                                                  aaaGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacat                                                                                                                              >  4:317790‑M1/1‑36 (MQ=255)
                                                                                                                    aggcacaattCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgac                     <  1:324205‑M1/147‑117 (MQ=255)
                                                                                                                    aGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttc                                                               >  1:856422‑M1/1‑34 (MQ=255)
                                                                                                                    aGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttc                                                               <  2:856422‑M1/108‑75 (MQ=255)
                                                                                                                     gTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacg                    >  2:783641‑M1/1‑33 (MQ=255)
                                                                                                                        acaATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggat                 >  2:575128‑M1/1‑30 (MQ=255)
                                                                                                                            ttCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcgtcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatcccc             <  3:659399‑M1/150‑125 (MQ=255)
                                                                                                                              ctcAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccgg           >  2:786762‑M1/1‑24 (MQ=255)
                                                                                                                               tcAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctatagaataggtacttctaactgcagtttgacgtatccccgga          <  4:81997‑M1/150‑128 (MQ=255)
                                                                                                                               tcAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccgga          >  4:163430‑M1/1‑23 (MQ=255)
                                                                                                                                cAAAATCAGGAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaa         >  4:764420‑M1/1‑22 (MQ=255)
                                                                                                                                cAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaa         <  1:1277983‑M1/150‑129 (MQ=255)
                                                                                                                                     tCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttctaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctc    <  2:1008195‑M1/150‑134 (MQ=255)
                                                                                                                                     tCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctc    <  4:343155‑M1/150‑134 (MQ=255)
                                                                                                                                     tCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctc    <  1:47637‑M1/150‑134 (MQ=255)
                                                                                                                                     tCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctc    <  1:176529‑M1/150‑134 (MQ=255)
                                                                                                                                     tCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctc    <  1:764024‑M1/150‑134 (MQ=255)
                                                                                                                                     tCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctc    <  2:330206‑M1/150‑134 (MQ=255)
                                                                                                                                       agaagaGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcga  >  1:1037287‑M1/1‑15 (MQ=255)
                                                                                                                                        gaagaGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctag                                                  >  3:770761‑M1/1‑14 (MQ=255)
                                                                                                                                        gaagaGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctag                                                  <  4:770761‑M1/101‑88 (MQ=255)
                                                                                                                                                     |                                                                                                                                       
GGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACC  >  NC_000913_3_KO_pgi/4233608‑4233892

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.