Predicted mutation
evidence seq id position mutation annotation gene description
JC NC_000913_3_KO_pgi 4,233,757 +ATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTAC intergenic (‑524/‑583) lysC ← / → yjbE lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_KO_pgi = 42337570 (0.000)89 (0.980)
+ATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTAC
58/188 0.6 100% intergenic (‑524/‑583) lysC/yjbE lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein
?NC_000913_3_KO_pgi 4233758 = 0 (0.000)intergenic (‑525/‑582) lysC/yjbE lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein

GGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA  >  NC_000913_3_KO_pgi/4233611‑4233906
                                                                                                                                                   |                                                                                                                                                    
actcaacattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccagcgcagaccgctgcctggcaggcactacGgt                                                                                                                                                    >  1:927326‑M2/148‑150 (MQ=255)
actcaacattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGgt                                                                                                                                                    >  4:113140‑M2/148‑150 (MQ=255)
  tcaacattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGgtgt                                                                                                                                                  >  1:1284158‑M2/146‑150 (MQ=255)
  tcaacattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGgtgt                                                                                                                                                  >  3:208064‑M2/146‑150 (MQ=255)
  tcaacattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGgtgt                                                                                                                                                  <  3:713989‑M2/5‑1 (MQ=255)
  tcaacattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGgtgt                                                                                                                                                  >  3:687149‑M2/146‑150 (MQ=255)
  tcaacattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGgtgt                                                                                                                                                  <  3:298646‑M2/5‑1 (MQ=255)
  tcaacattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGgtgt                                                                                                                                                  >  4:688006‑M2/146‑150 (MQ=255)
   caacattacgctaacggcactacaaccatcccatttttctgtgactggcgctacaatcttacaaagtcacaattctccagatcagaagagtattgcgaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTa                                                                                                                                                 <  2:853286‑M2/6‑1 (MQ=255)
    aacattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAg                                                                                                                                                >  1:249457‑M2/144‑150 (MQ=255)
    aacattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAg                                                                                                                                                <  1:1096141‑M2/7‑1 (MQ=255)
    aacattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattactaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGGGTAg                                                                                                                                                >  1:108645‑M2/144‑150 (MQ=255)
     acattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctgaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAgg                                                                                                                                               <  1:577423‑M2/8‑1 (MQ=255)
     acattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAgg                                                                                                                                               <  1:1319724‑M2/8‑1 (MQ=255)
      cattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGc                                                                                                                                              >  4:445262‑M2/142‑150 (MQ=255)
        ttacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTg                                                                                                                                            <  2:628214‑M2/11‑1 (MQ=255)
         tacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTgg                                                                                                                                           <  3:597290‑M2/12‑1 (MQ=255)
          acgctaacggcactaaaacaatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGa                                                                                                                                          <  2:1147037‑M2/13‑1 (MQ=255)
            gctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAgc                                                                                                                                        <  2:258564‑M2/15‑1 (MQ=255)
             ctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAgct                                                                                                                                       >  1:256567‑M2/135‑150 (MQ=255)
             ctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAgct                                                                                                                                       <  3:141215‑M2/16‑1 (MQ=255)
              taacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAgctg                                                                                                                                      >  3:872045‑M2/134‑150 (MQ=255)
               aacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAgctgc                                                                                                                                     <  1:521717‑M2/18‑1 (MQ=255)
               aacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAgctgc                                                                                                                                     <  1:959536‑M2/18‑1 (MQ=255)
                  ggcactaaaaccatcacatttttctgttacttgcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTc                                                                                                                                  >  2:917513‑M2/130‑150 (MQ=255)
                  ggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTc                                                                                                                                  >  3:19144‑M2/130‑150 (MQ=255)
                     actaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGaa                                                                                                                               <  2:592024‑M2/24‑1 (MQ=255)
                     actaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGGGTAGGCTGGAGCTGCTTCGaa                                                                                                                               >  1:483143‑M2/127‑150 (MQ=255)
                     actaaaaccatcacatttttctgggactggcgcgacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGaa                                                                                                                               <  2:940612‑M2/24‑1 (MQ=255)
                      ctaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAg                                                                                                                              <  3:113140‑M2/25‑1 (MQ=255)
                       taaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagacggctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGt                                                                                                                             >  1:799768‑M2/125‑150 (MQ=255)
                       taaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGt                                                                                                                             >  3:28047‑M2/125‑150 (MQ=255)
                         aaaccatcacatttttctttgatttgctttacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTc                                                                                                                           >  4:409068‑M2/123‑150 (MQ=255)
                         aaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTc                                                                                                                           >  4:838297‑M2/123‑150 (MQ=255)
                             catcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCtat                                                                                                                       >  2:1092793‑M2/119‑150 (MQ=255)
                               tcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATAc                                                                                                                     >  2:683078‑M2/117‑150 (MQ=255)
                               tcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATAc                                                                                                                     >  2:46477‑M2/117‑150 (MQ=255)
                                  catttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACttt                                                                                                                  <  4:623857‑M2/37‑1 (MQ=255)
                                   atttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTc                                                                                                                 <  1:653619‑M2/38‑1 (MQ=255)
                                   atttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTc                                                                                                                 <  4:119645‑M2/38‑1 (MQ=255)
                                   atttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTc                                                                                                                 >  4:351522‑M2/113‑150 (MQ=255)
                                       ttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTaga                                                                                                             <  1:421549‑M2/42‑1 (MQ=255)
                                           gtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAAt                                                                                                         <  3:722730‑M2/46‑1 (MQ=255)
                                            tgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATa                                                                                                        <  3:886828‑M2/47‑1 (MQ=255)
                                            tgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATa                                                                                                        >  4:886828‑M2/104‑150 (MQ=255)
                                             gactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAg                                                                                                       <  3:727176‑M2/48‑1 (MQ=255)
                                              actggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAgg                                                                                                      <  4:619940‑M2/49‑1 (MQ=255)
                                              actggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAgg                                                                                                      <  1:510978‑M2/49‑1 (MQ=255)
                                                tggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGaa                                                                                                    >  3:788506‑M2/100‑150 (MQ=255)
                                                tggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGaa                                                                                                    >  3:788635‑M2/100‑150 (MQ=255)
                                                 ggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAAc                                                                                                   <  1:1152550‑M2/52‑1 (MQ=255)
                                                 ggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAAc                                                                                                   <  2:128654‑M2/52‑1 (MQ=255)
                                                  gcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACt                                                                                                  >  1:771345‑M2/98‑150 (MQ=255)
                                                  gcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACt                                                                                                  >  3:285917‑M2/98‑150 (MQ=255)
                                                   cgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACtt                                                                                                 <  1:1136376‑M2/54‑1 (MQ=255)
                                                    gctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTc                                                                                                <  1:1089793‑M2/55‑1 (MQ=255)
                                                    gctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTc                                                                                                <  4:668124‑M2/55‑1 (MQ=255)
                                                       acaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGaa                                                                                             <  2:1284158‑M2/58‑1 (MQ=255)
                                                       acaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGaa                                                                                             <  1:20774‑M2/58‑1 (MQ=255)
                                                          atcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTg                                                                                          <  2:600538‑M2/61‑1 (MQ=255)
                                                            cttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCa                                                                                        <  3:688006‑M2/63‑1 (MQ=255)
                                                               ccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGGGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGt                                                                                     >  1:966901‑M2/85‑150 (MQ=255)
                                                               ccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGt                                                                                     <  3:838297‑M2/66‑1 (MQ=255)
                                                               ccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGt                                                                                     <  2:739646‑M2/66‑1 (MQ=255)
                                                                   agtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTc                                                                                                                           >  1:856422‑M2/81‑108 (MQ=255)
                                                                   agtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTc                                                                                                                           <  2:856422‑M2/28‑1 (MQ=255)
                                                                   aggcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGAc                                                                                 <  1:324205‑M2/70‑1 (MQ=255)
                                                                    gtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACg                                                                                >  2:783641‑M2/80‑150 (MQ=255)
                                                                       acaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGAt                                                                             >  2:575128‑M2/77‑150 (MQ=255)
                                                                           ttctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCGTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATcccc                                                                         <  3:659399‑M2/78‑1 (MQ=255)
                                                                             ctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCgg                                                                       >  2:786762‑M2/71‑150 (MQ=255)
                                                                              tcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTATAGAATAGGTACTTCTAACTGCAGTTTGACGTATCCCCGGa                                                                      <  4:81997‑M2/81‑1 (MQ=255)
                                                                              tcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGa                                                                      >  4:163430‑M2/70‑150 (MQ=255)
                                                                               caaaatcaggagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGaa                                                                     >  4:764420‑M2/69‑150 (MQ=255)
                                                                               caaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGaa                                                                     >  3:684894‑M2/69‑150 (MQ=255)
                                                                               caaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGaa                                                                     <  1:1277983‑M2/82‑1 (MQ=255)
                                                                                 acatcagaacggtattgctaatgaaaaacatcaatccaacgccgatcgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCCATACTTTCTAGAGGATGGGACCTTCGAACTGCAGGTCGACGGATCCCCGGaatg                                                                   <  4:19144‑M2/84‑2 (MQ=255)
                                                                                   atcagaagagtattgctaatgaaaaacatcaattcaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGACAATAGGAACTTCGAACTGCGCGTCGACCGAACcccggattgct                                                                 <  4:801311‑M2/86‑6 (MQ=255)
                                                                                    tcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCTAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCcggaatgctc                                                                <  2:1008195‑M2/87‑5 (MQ=255)
                                                                                    tcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCcggaatgctc                                                                <  1:764024‑M2/87‑5 (MQ=255)
                                                                                    tcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCcggaatgctc                                                                <  2:330206‑M2/87‑5 (MQ=255)
                                                                                    tcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCcggaatgctc                                                                <  1:176529‑M2/87‑5 (MQ=255)
                                                                                    tcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCcggaatgctc                                                                <  4:343155‑M2/87‑5 (MQ=255)
                                                                                    tcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCcggaatgctc                                                                <  1:47637‑M2/87‑5 (MQ=255)
                                                                                     cagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGaa                                                                                             >  2:804133‑M2/63‑120 (MQ=255)
                                                                                     cagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGaa                                                                                             <  1:804133‑M2/58‑1 (MQ=255)
                                                                                      agaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCcccggaatgctcga                                                              >  1:1037287‑M2/62‑144 (MQ=255)
                                                                                       gaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTag                                                                                                              <  4:770761‑M2/41‑1 (MQ=255)
                                                                                       gaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTag                                                                                                              >  3:770761‑M2/61‑101 (MQ=255)
                                                                                                                                             cactacggtgtaggCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatg       >  2:268881‑M1/7‑89 (MQ=255)
                                                                                                                                              actacggtgtagGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgt      >  3:312540‑M1/6‑88 (MQ=255)
                                                                                                                                               ctacggtgtaGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgta     <  2:1306065‑M1/146‑64 (MQ=255)
                                                                                                                                                tacggtgtAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtag    >  4:24956‑M1/4‑86 (MQ=255)
                                                                                                                                                tacggtgtAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtag    >  2:215612‑M1/4‑86 (MQ=255)
                                                                                                                                                 acggtgTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtagg   <  1:718586‑M1/148‑66 (MQ=255)
                                                                                                                                                  cggtGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggc  >  4:717888‑M1/2‑84 (MQ=255)
                                                                                                                                                  cggtGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggc  <  3:595899‑M1/149‑67 (MQ=255)
                                                                                                                                                  cggtGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccg                                           >  2:1271485‑M1/2‑84 (MQ=255)
                                                                                                                                                  cggtGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccg                                           <  1:1271485‑M1/108‑26 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA  >  NC_000913_3_KO_pgi/4233611‑4233906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.