New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_KO_pgi	= 859192	92 (1.050)	7 (0.080)	7/264	11.8	7.2%	intergenic (‑131/+21)	ybiU/ybiV	DUF1479 family protein/sugar phosphatase; fructose‑1‑P/ribose‑5‑P/glucose‑6‑P phosphatase
	?	NC_000913_3_KO_pgi	= 859197	93 (1.120)					intergenic (‑136/+16)	ybiU/ybiV	DUF1479 family protein/sugar phosphatase; fructose‑1‑P/ribose‑5‑P/glucose‑6‑P phosphatase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

AAAAGTAGAAGCCATAACCACCTCTCATTAACTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCGGTAGCGTAACGGGCGATTTGTTTAATGTTTTCCGCAGCATTGCCCATCGCAAA  >  NC_000913_3_KO_pgi/859047‑859341                                                                                                                                                  |                                                                                                                                                      aaaaGTAGAAGCCATAACCACCTCTCATTAACTTTCATTCGAAACCATGCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCAc                                                                                                                                                   >  2:612103/1‑150 (MQ=255)       aGAAGCCATAACCACCTCTCATTAACTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTcc                                                                                                                                             >  1:882245/1‑150 (MQ=255)         aaGCCATAACCACCTCTCATTAACTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCgg                                                                                                                                           <  2:256666/150‑1 (MQ=255)            ccATAACCACCTCTCATTAACTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTag                                                                                                                                        >  4:362590/1‑150 (MQ=255)               tAACCACCTCTCATTAACTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTagaga                                                                                                                                     >  2:618369/1‑150 (MQ=255)                aaCCACCTCTCATTAACTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCCCGGCTCCGGTagagag                                                                                                                                    <  2:463121/150‑1 (MQ=255)                     ccTCTCATTAACTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAg                                                                                                                               <  1:104737/150‑1 (MQ=255)                        ctcATTAACTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTg                                                                                                                            >  4:250138/1‑150 (MQ=255)                            ttAACTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTGTCATGATTTGATGGGATGTGCAGGAGGTAAAGAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTGaa                                                                                                                        >  1:228235/1‑150 (MQ=255)                                cTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAgg                                                                                                                    <  4:262205/150‑1 (MQ=255)                                 tttCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAggg                                                                                                                   >  4:410163/1‑150 (MQ=255)                                 tttCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAggg                                                                                                                   >  3:340047/1‑150 (MQ=255)                                    cATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGAGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGAt                                                                                                                >  3:188297/1‑150 (MQ=255)                                     aTTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGAtg                                                                                                               <  2:811164/150‑1 (MQ=255)                                     aTTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGAtg                                                                                                               <  1:324172/150‑1 (MQ=255)                                      ttCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGAtgt                                                                                                              <  1:865615/150‑1 (MQ=255)                                        cGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATgtgt                                                                                                            <  1:76613/150‑1 (MQ=255)                                         gAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGtt                                                                                                           >  2:596309/1‑150 (MQ=255)                                         gAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGtt                                                                                                           >  3:95915/1‑150 (MQ=255)                                          aaaCCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTa                                                                                                          >  2:669490/1‑150 (MQ=255)                                          aaaCCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTa                                                                                                          <  2:263441/150‑1 (MQ=255)                                              cATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCa                                                                                                      >  4:179850/1‑150 (MQ=255)                                               aTTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAg                                                                                                     >  1:347943/1‑150 (MQ=255)                                                ttCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGc                                                                                                    <  2:778533/150‑1 (MQ=255)                                                     aTTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTAt                                                                                                         <  2:34019/140‑1 (MQ=255)                                                     aTTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTAt                                                                                                         >  1:34019/1‑140 (MQ=255)                                                     aTTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGc                                                                                               >  1:847476/1‑150 (MQ=255)                                                     aTTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGc                                                                                               >  1:382686/1‑150 (MQ=255)                                                      tttGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGcc                                                                                              <  1:552621/150‑1 (MQ=255)                                                         tcATGTGACCATATAAGTTATAGATGAGTTAATGCAAGTTTAATGATTTGCCGTGATGCACCGGCGGTAAAAAGAAGGCAAACCCGGAGCGAACAGCTACGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTCTAATCCAGCTCCGCCTGa                                                                                           <  2:521573/149‑1 (MQ=255)                                                         gCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGa                                                                                           <  2:689870/150‑1 (MQ=255)                                                          cATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTGGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGaa                                                                                          <  1:9511/150‑1 (MQ=255)                                                            tgtgACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATc                                                                                        >  1:371202/1‑150 (MQ=255)                                                             gtgACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTNAATca                                                                                       >  3:186272/1‑150 (MQ=255)                                                             gtgACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTNAATca                                                                                       >  3:186281/1‑150 (MQ=255)                                                              tgACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATcac                                                                                      >  2:470282/1‑150 (MQ=255)                                                               gACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCGCGGCTTCGGAAGAGAGGTAAGCTGTCAAAAGGGGATGGGTGATCCAGCACCGCCTGCATcacc                                                                                     >  2:474900/1‑149 (MQ=255)                                                               gACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATcaca                                                                                     <  1:885588/150‑1 (MQ=255)                                                               gACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATcaca                                                                                     >  3:369592/1‑150 (MQ=255)                                                               gACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATcaca                                                                                     <  2:198845/150‑1 (MQ=255)                                                                aCAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACAt                                                                                    >  3:275475/1‑150 (MQ=255)                                                                   atatACGTTATAAATAAGTTAATGCACGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAATACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCa                                                                                 >  2:303116/1‑150 (MQ=255)                                                                         gTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAgc                                                                                                                                                       >  3:352742/1‑74 (MQ=255)                                                                         gTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAgc                                                                                                                                                       <  4:352742/74‑1 (MQ=255)                                                                         gTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAgcgcgc                                                                           >  4:196499/1‑150 (MQ=255)                                                                          ttATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGcc                                                                          >  2:347711/1‑150 (MQ=255)                                                                            ataaataaGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCtt                                                                        <  3:306246/150‑1 (MQ=255)                                                                                  aaGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGat                                                                  >  4:83023/1‑150 (MQ=255)                                                                                    gTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGatta                                                                <  4:573003/150‑1 (MQ=255)                                                                                    gTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAAGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGatta                                                                <  3:307140/150‑1 (MQ=255)                                                                                        aTGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATtattat                                                            <  1:437064/150‑1 (MQ=255)                                                                                          gCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTatca                                                          >  1:287793/1‑150 (MQ=255)                                                                                            aaGTTTAGTGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTatcatc                                                        >  2:919803/1‑150 (MQ=255)                                                                                            aaGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTatcatc                                                        >  3:42192/1‑150 (MQ=255)                                                                                            aaGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTatcatc                                                        >  1:81791/1‑150 (MQ=255)                                                                                             aGTTTAATGATTGGATGTGATGTGCAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGCCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTGATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTAGTATCATCg                                                       <  1:474900/150‑1 (MQ=255)                                                                                              gTTTAATGATTTGATGGGATAAACAGGAAGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCgg                                                      >  4:267464/1‑150 (MQ=255)                                                                                               tttAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAgggg                                                                                                                  <  4:198760/89‑1 (MQ=255)                                                                                               tttAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAgggg                                                                                                                  >  3:198760/1‑89 (MQ=255)                                                                                                ttAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCGGTa                                                    <  3:465088/150‑1 (MQ=255)                                                                                                  aaTGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCGGTAGc                                                  >  2:788021/1‑150 (MQ=255)                                                                                                  aaTGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGGCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCGGTAGc                                                  >  3:180729/1‑150 (MQ=255)                                                                                                    tGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCGGTAGCGt                                                >  2:861585/1‑150 (MQ=255)                                                                                                    tGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCGGTAGCGt                                                >  4:357348/1‑150 (MQ=255)                                                                                                       tttGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCGGTAGCGTAAc                                             >  2:741944/1‑150 (MQ=255)                                                                                                           atgtgatgtACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCGGTAGCGTAACGGGc                                         <  2:382686/150‑1 (MQ=255)                                                                                                           atgtgatgtACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCGGTAGCGTAACGGGc                                         <  2:847476/150‑1 (MQ=255)                                                                                                                 tgtACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAg                                                                                                                               <  4:532937/58‑1 (MQ=255)                                                                                                                 tgtACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAg                                                                                                                               >  3:532937/1‑58 (MQ=255)                                                                                                                   tACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCGGTAGCGTAACGGGCGATTTGtt                                 >  2:486297/1‑150 (MQ=255)                                                                                                                    aCAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCGGTAGCGTAACGGGCGATTTGttt                                <  3:576052/150‑1 (MQ=255)                                                                                                                    aCAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCGGTAGCGTAACGGGCGATTTGttt                                <  1:69939/150‑1 (MQ=255)                                                                                                                    aCAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCAGAATTACGATCACCAGTACCGTAACGGGCGATTTGatt                                <  2:894998/150‑1 (MQ=255)                                                                                                                      aGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGTTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCGGTAGCGTAACGGGCGATTTGTTTaa                              <  2:180076/150‑1 (MQ=255)                                                                                                                      aGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCGGTAGCGTAACGGGCGATTTGTTTaa                              <  2:174959/150‑1 (MQ=255)                                                                                                                        gATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCGGTAGCGTAACGGGCGATTTGTTTAATg                            >  1:675758/1‑150 (MQ=255)                                                                                                                             aaaaaGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCGGTAGCGTAACGGGCGATTTGTTTAATGTTTTc                       <  4:327208/150‑1 (MQ=255)                                                                                                                              aaaaGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCGGTAGCGTAACGGGCGATTTGTTTAATGTTTTcc                      >  1:122173/1‑150 (MQ=255)                                                                                                                               aaaGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCGGTAGCGTAACGGGCGATTTGTTTAATGTTTTCCg                     >  1:583929/1‑150 (MQ=255)                                                                                                                                aagaagGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCGGTAGCGTAACGGGCGATTTGTTTAATGTTTTCCgc                    <  4:167282/150‑1 (MQ=255)                                                                                                                                aagaagGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCGGTAGCGTAACGGGCGATTTGTTTAATGTTTTCCgc                    >  3:289363/1‑150 (MQ=255)                                                                                                                                 agaagGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCGGTAGCGTAACGGGCGATTTGTTTAATGTTTTCCgca                   >  3:377989/1‑150 (MQ=255)                                                                                                                                  gaagGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTCAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCGGTAGCGTAACGGGCGGTTTGTTTAATGTTTTCCgcag                  >  4:227654/1‑150 (MQ=255)                                                                                                                                  gaagGGAAAACCGGAGCGCACGACTCCGGTAGAGAGGGCAGCTGTTAAAAGGGGCTGTGTTATCCAGCACCGCCTGAATCACATTCAGCCCGCCTGCAAGATTATTATCATCGGTAGCGTAACGGGCGATTTGTCTAATGTTTTCCgcag                  >  4:295634/1‑150 (MQ=255)                                                                                                                                      ggAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCGGTAGCGTAACGGGCGATTTGTTTAATGTTTTCCGCAGCAtt              >  2:738773/1‑150 (MQ=255)                                                                                                                                      ggAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCGGTAGCGTAACGGGCGATTTGTTTAATGTTTTCCGCAGCAtt              >  2:738913/1‑150 (MQ=255)                                                                                                                                       gAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCGGTAGCGTAACGGGCGATTTGTTTAATGTTTTCCGCAGCATTg             >  1:433448/1‑150 (MQ=255)                                                                                                                                       gAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCGGTAGCGTAACGGGCGATTTGTTTAATGTTTTCCGCAGCATTg             >  1:869375/1‑150 (MQ=255)                                                                                                                                         aaaCCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCGGTAGCGTAACGGGCGATTTGTTTAATGTTTTCCGCAGCATTGcc           <  1:899364/150‑1 (MQ=255)                                                                                                                                          atccGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTCTCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCGGTAGCGTAACGGGCGATTTGTCTAATGTTTTCCGCAGCATTGccc          <  2:620010/148‑1 (MQ=255)                                                                                                                                          aaCCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCGGTAGCGTAACGGGCGATTTGTTTAATGTTTTCCGCAGCATTGccc          >  1:830867/1‑150 (MQ=255)                                                                                                                                          aaCCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCGGTAGCGTAACGGGCGATTTGTTTAATGTTTTCCGCAGCATTGccc          <  1:303116/150‑1 (MQ=255)                                                                                                                                             cGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCGGTAGCGTAACGGGCGATTTGTTTAATGTTTTCCGCAGCATTGCCCATc       <  1:109626/150‑1 (MQ=255)                                                                                                                                                  cgcACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCGGTAGCGTAACGGGCGATTTGTTTAATGTTTTCCGCAGCATTGCCCATCGCaaa  <  1:98021/150‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                      AAAAGTAGAAGCCATAACCACCTCTCATTAACTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCGGTAGCGTAACGGGCGATTTGTTTAATGTTTTCCGCAGCATTGCCCATCGCAAA  >  NC_000913_3_KO_pgi/859047‑859341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.