New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_KO_pgi	= 859192	92 (1.050)	7 (0.080)	7/264	11.8	7.2%	intergenic (‑131/+21)	ybiU/ybiV	DUF1479 family protein/sugar phosphatase; fructose‑1‑P/ribose‑5‑P/glucose‑6‑P phosphatase
	?	NC_000913_3_KO_pgi	= 859197	93 (1.120)					intergenic (‑136/+16)	ybiU/ybiV	DUF1479 family protein/sugar phosphatase; fructose‑1‑P/ribose‑5‑P/glucose‑6‑P phosphatase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

AGAAGCCATAACCACCTCTCATTAACTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCGGTAGCGTAACGGGCGATTTGTTTAATGTTTTCCGCAGCATTGCCCATCGCAAAGGAAT  >  NC_000913_3_KO_pgi/859053‑859346                                                                                                                                                 |                                                                                                                                                      aGAAGCCATAACCACCTCTCATTAACTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTcc                                                                                                                                                  >  1:882245/1‑150 (MQ=255)   aaGCCATAACCACCTCTCATTAACTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCgg                                                                                                                                                <  2:256666/150‑1 (MQ=255)      ccATAACCACCTCTCATTAACTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTag                                                                                                                                             >  4:362590/1‑150 (MQ=255)         tAACCACCTCTCATTAACTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTagaga                                                                                                                                          >  2:618369/1‑150 (MQ=255)          aaCCACCTCTCATTAACTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCCCGGCTCCGGTagagag                                                                                                                                         <  2:463121/150‑1 (MQ=255)               ccTCTCATTAACTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAg                                                                                                                                    <  1:104737/150‑1 (MQ=255)                  ctcATTAACTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTg                                                                                                                                 >  4:250138/1‑150 (MQ=255)                      ttAACTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTGTCATGATTTGATGGGATGTGCAGGAGGTAAAGAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTGaa                                                                                                                             >  1:228235/1‑150 (MQ=255)                          cTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAgg                                                                                                                         <  4:262205/150‑1 (MQ=255)                           tttCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAggg                                                                                                                        >  4:410163/1‑150 (MQ=255)                           tttCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAggg                                                                                                                        >  3:340047/1‑150 (MQ=255)                              cATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGAGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGAt                                                                                                                     >  3:188297/1‑150 (MQ=255)                               aTTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGAtg                                                                                                                    <  1:324172/150‑1 (MQ=255)                               aTTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGAtg                                                                                                                    <  2:811164/150‑1 (MQ=255)                                ttCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGAtgt                                                                                                                   <  1:865615/150‑1 (MQ=255)                                  cGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATgtgt                                                                                                                 <  1:76613/150‑1 (MQ=255)                                   gAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGtt                                                                                                                >  3:95915/1‑150 (MQ=255)                                   gAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGtt                                                                                                                >  2:596309/1‑150 (MQ=255)                                    aaaCCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTa                                                                                                               <  2:263441/150‑1 (MQ=255)                                    aaaCCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTa                                                                                                               >  2:669490/1‑150 (MQ=255)                                        cATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCa                                                                                                           >  4:179850/1‑150 (MQ=255)                                         aTTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAg                                                                                                          >  1:347943/1‑150 (MQ=255)                                          ttCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGc                                                                                                         <  2:778533/150‑1 (MQ=255)                                               aTTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTAt                                                                                                              <  2:34019/140‑1 (MQ=255)                                               aTTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTAt                                                                                                              >  1:34019/1‑140 (MQ=255)                                               aTTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGc                                                                                                    >  1:847476/1‑150 (MQ=255)                                               aTTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGc                                                                                                    >  1:382686/1‑150 (MQ=255)                                                tttGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGcc                                                                                                   <  1:552621/150‑1 (MQ=255)                                                   tcATGTGACCATATAAGTTATAGATGAGTTAATGCAAGTTTAATGATTTGCCGTGATGCACCGGCGGTAAAAAGAAGGCAAACCCGGAGCGAACAGCTACGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTCTAATCCAGCTCCGCCTGa                                                                                                <  2:521573/149‑1 (MQ=255)                                                   gCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGa                                                                                                <  2:689870/150‑1 (MQ=255)                                                    cATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTGGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGaa                                                                                               <  1:9511/150‑1 (MQ=255)                                                      tgtgACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATc                                                                                             >  1:371202/1‑150 (MQ=255)                                                       gtgACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTNAATca                                                                                            >  3:186272/1‑150 (MQ=255)                                                       gtgACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTNAATca                                                                                            >  3:186281/1‑150 (MQ=255)                                                        tgACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATcac                                                                                           >  2:470282/1‑150 (MQ=255)                                                         gACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCGCGGCTTCGGAAGAGAGGTAAGCTGTCAAAAGGGGATGGGTGATCCAGCACCGCCTGCATcacc                                                                                          >  2:474900/1‑149 (MQ=255)                                                         gACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATcaca                                                                                          <  1:885588/150‑1 (MQ=255)                                                         gACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATcaca                                                                                          <  2:198845/150‑1 (MQ=255)                                                         gACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATcaca                                                                                          >  3:369592/1‑150 (MQ=255)                                                          aCAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACAt                                                                                         >  3:275475/1‑150 (MQ=255)                                                             atatACGTTATAAATAAGTTAATGCACGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAATACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCa                                                                                      >  2:303116/1‑150 (MQ=255)                                                                   gTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAgcgcgc                                                                                >  4:196499/1‑150 (MQ=255)                                                                    ttATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGcc                                                                               >  2:347711/1‑150 (MQ=255)                                                                      ataaataaGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCtt                                                                             <  3:306246/150‑1 (MQ=255)                                                                            aaGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGat                                                                       >  4:83023/1‑150 (MQ=255)                                                                              gTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGatta                                                                     <  4:573003/150‑1 (MQ=255)                                                                              gTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAAGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGatta                                                                     <  3:307140/150‑1 (MQ=255)                                                                                  aTGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATtattat                                                                 <  1:437064/150‑1 (MQ=255)                                                                                    gCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTatca                                                               >  1:287793/1‑150 (MQ=255)                                                                                      aaGTTTAGTGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTatcatc                                                             >  2:919803/1‑150 (MQ=255)                                                                                      aaGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTatcatc                                                             >  3:42192/1‑150 (MQ=255)                                                                                      aaGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTatcatc                                                             >  1:81791/1‑150 (MQ=255)                                                                                       aGTTTAATGATTGGATGTGATGTGCAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGCCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTGATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTAGTATCATCg                                                            <  1:474900/150‑1 (MQ=255)                                                                                        gTTTAATGATTTGATGGGATAAACAGGAAGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCgg                                                           >  4:267464/1‑150 (MQ=255)                                                                                         tttAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAgggg                                                                                                                       >  3:198760/1‑89 (MQ=255)                                                                                         tttAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAgggg                                                                                                                       <  4:198760/89‑1 (MQ=255)                                                                                          ttAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCGGTa                                                         <  3:465088/150‑1 (MQ=255)                                                                                            aaTGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCGGTAGc                                                       >  2:788021/1‑150 (MQ=255)                                                                                            aaTGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGGCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCGGTAGc                                                       >  3:180729/1‑150 (MQ=255)                                                                                              tGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCGGTAGCGt                                                     >  2:861585/1‑150 (MQ=255)                                                                                              tGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCGGTAGCGt                                                     >  4:357348/1‑150 (MQ=255)                                                                                                 tttGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCGGTAGCGTAAc                                                  >  2:741944/1‑150 (MQ=255)                                                                                                     atgtgatgtACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCGGTAGCGTAACGGGc                                              <  2:382686/150‑1 (MQ=255)                                                                                                     atgtgatgtACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCGGTAGCGTAACGGGc                                              <  2:847476/150‑1 (MQ=255)                                                                                                           tgtACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAg                                                                                                                                    <  4:532937/58‑1 (MQ=255)                                                                                                           tgtACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAg                                                                                                                                    >  3:532937/1‑58 (MQ=255)                                                                                                             tACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCGGTAGCGTAACGGGCGATTTGtt                                      >  2:486297/1‑150 (MQ=255)                                                                                                              aCAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCGGTAGCGTAACGGGCGATTTGttt                                     <  3:576052/150‑1 (MQ=255)                                                                                                              aCAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCGGTAGCGTAACGGGCGATTTGttt                                     <  1:69939/150‑1 (MQ=255)                                                                                                              aCAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCAGAATTACGATCACCAGTACCGTAACGGGCGATTTGatt                                     <  2:894998/150‑1 (MQ=255)                                                                                                                aGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGTTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCGGTAGCGTAACGGGCGATTTGTTTaa                                   <  2:180076/150‑1 (MQ=255)                                                                                                                aGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCGGTAGCGTAACGGGCGATTTGTTTaa                                   <  2:174959/150‑1 (MQ=255)                                                                                                                  gATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCGGTAGCGTAACGGGCGATTTGTTTAATg                                 >  1:675758/1‑150 (MQ=255)                                                                                                                       aaaaaGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCGGTAGCGTAACGGGCGATTTGTTTAATGTTTTc                            <  4:327208/150‑1 (MQ=255)                                                                                                                        aaaaGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCGGTAGCGTAACGGGCGATTTGTTTAATGTTTTcc                           >  1:122173/1‑150 (MQ=255)                                                                                                                         aaaGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCGGTAGCGTAACGGGCGATTTGTTTAATGTTTTCCg                          >  1:583929/1‑150 (MQ=255)                                                                                                                          aagaagGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCGGTAGCGTAACGGGCGATTTGTTTAATGTTTTCCgc                         >  3:289363/1‑150 (MQ=255)                                                                                                                          aagaagGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCGGTAGCGTAACGGGCGATTTGTTTAATGTTTTCCgc                         <  4:167282/150‑1 (MQ=255)                                                                                                                           agaagGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCGGTAGCGTAACGGGCGATTTGTTTAATGTTTTCCgca                        >  3:377989/1‑150 (MQ=255)                                                                                                                            gaagGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTCAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCGGTAGCGTAACGGGCGGTTTGTTTAATGTTTTCCgcag                       >  4:227654/1‑150 (MQ=255)                                                                                                                            gaagGGAAAACCGGAGCGCACGACTCCGGTAGAGAGGGCAGCTGTTAAAAGGGGCTGTGTTATCCAGCACCGCCTGAATCACATTCAGCCCGCCTGCAAGATTATTATCATCGGTAGCGTAACGGGCGATTTGTCTAATGTTTTCCgcag                       >  4:295634/1‑150 (MQ=255)                                                                                                                                ggAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCGGTAGCGTAACGGGCGATTTGTTTAATGTTTTCCGCAGCAtt                   >  2:738773/1‑150 (MQ=255)                                                                                                                                ggAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCGGTAGCGTAACGGGCGATTTGTTTAATGTTTTCCGCAGCAtt                   >  2:738913/1‑150 (MQ=255)                                                                                                                                 gAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCGGTAGCGTAACGGGCGATTTGTTTAATGTTTTCCGCAGCATTg                  >  1:869375/1‑150 (MQ=255)                                                                                                                                 gAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCGGTAGCGTAACGGGCGATTTGTTTAATGTTTTCCGCAGCATTg                  >  1:433448/1‑150 (MQ=255)                                                                                                                                   aaaCCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCGGTAGCGTAACGGGCGATTTGTTTAATGTTTTCCGCAGCATTGcc                <  1:899364/150‑1 (MQ=255)                                                                                                                                    atccGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTCTCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCGGTAGCGTAACGGGCGATTTGTCTAATGTTTTCCGCAGCATTGccc               <  2:620010/148‑1 (MQ=255)                                                                                                                                    aaCCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCGGTAGCGTAACGGGCGATTTGTTTAATGTTTTCCGCAGCATTGccc               >  1:830867/1‑150 (MQ=255)                                                                                                                                    aaCCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCGGTAGCGTAACGGGCGATTTGTTTAATGTTTTCCGCAGCATTGccc               <  1:303116/150‑1 (MQ=255)                                                                                                                                       cGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCGGTAGCGTAACGGGCGATTTGTTTAATGTTTTCCGCAGCATTGCCCATc            <  1:109626/150‑1 (MQ=255)                                                                                                                                            cgcACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCGGTAGCGTAACGGGCGATTTGTTTAATGTTTTCCGCAGCATTGCCCATCGCaaa       <  1:98021/150‑1 (MQ=255)                                                                                                                                             gcACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCGGTAGCGTAACGGGCGATTTGTTTAATGTTTTCCGCAGCATTGCCCATCGCAAAg      <  1:596309/150‑1 (MQ=255)                                                                                                                                                 ggCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCGGTAGCGTAACGGGCGATTTGTTTAATGTTTTCCGCAGCATTGCCCATCGCAAAGGAAt  >  3:355659/1‑150 (MQ=255)                                                                                                                                                 ggCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCGGTAGCGTAACGGGCGATTTGTTTAATGTTTTCCGCAGCATTGCCCATCGCAAAGGAAt  <  2:287793/150‑1 (MQ=255)                                                                                                                                                 ggCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCGGTAGCGTAACGGGCGATTTGTTTAATGTTTTCCGCAGCATTGCCCATCGCAAAGGAAt  >  4:555630/1‑150 (MQ=255)                                                                                                                                                 |                                                                                                                                                      AGAAGCCATAACCACCTCTCATTAACTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGTAAAAAGAAGGGAAAACCGGAGCGCACGGCTCCGGTAGAGAGGTCAGCTGTTAAAAGGGGATGTGTTATCCAGCACCGCCTGAATCACATTCAGCGCGCCTTCATGATTATTATCATCGGTAGCGTAACGGGCGATTTGTTTAATGTTTTCCGCAGCATTGCCCATCGCAAAGGAAT  >  NC_000913_3_KO_pgi/859053‑859346

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.