New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_KO_pgi 4383971 =114 (0.470)133 (0.540) 95/282 3.6 53.8% coding (145/315 nt) yjeN uncharacterized protein
?NC_000913_3_KO_pgi = 4497945 NA (NA)intergenic (+11/+82) insD1/yjgX IS2 transposase TnpB/pseudogene fragment
Rejected: Coverage evenness skew score above cutoff.

Only 100 of 118 total aligned reads displayed.

ATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAA  >  NC_000913_3_KO_pgi/4383822‑4384118
                                                                                                                                                     |                                                                                                                                                   
aTGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGAtggtggtgg                                                                                                                                                     <  2:1010018/150‑1 (MQ=255)
 tGTTATGGATGACACCAGCCGGTACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTggg                                                                                                                                                    <  1:561295/150‑1 (MQ=255)
   ttATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTa                                                                                                                                                  <  4:102322/150‑1 (MQ=255)
   ttATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTa                                                                                                                                                  >  1:2005348/1‑150 (MQ=255)
     aTGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGc                                                                                                                                                <  1:2538056/150‑1 (MQ=255)
      tGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAgta                                                                                                                                               >  4:70162/1‑148 (MQ=255)
       ggATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCAc                                                                                                                                              <  3:710824/150‑1 (MQ=255)
        gATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAgtaga                                                                                                                                             >  1:1361152/1‑146 (MQ=255)
        gATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAgtaga                                                                                                                                             >  2:2451849/1‑146 (MQ=255)
        gATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCAcc                                                                                                                                             >  1:1131139/1‑150 (MQ=255)
         aTGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCa                                                                                                                                            >  1:88649/1‑150 (MQ=255)
           gACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCACTTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACGCGTAAAGTGGCGCTGGTGGTGGGTAgtcgcctg                                                                                                                                          >  2:764119/1‑143 (MQ=255)
           gACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAgtagactg                                                                                                                                          <  2:1177866/150‑8 (MQ=255)
           gACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAgtagactg                                                                                                                                          >  2:2153012/1‑143 (MQ=255)
           gACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCAtt                                                                                                                                          <  2:1544498/150‑1 (MQ=255)
           gACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGCGGGTAgtagactg                                                                                                                                          <  2:1130883/150‑8 (MQ=255)
           gACACCAGCCGGGACCCGGCAATAAACGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAgtagactg                                                                                                                                          <  2:387704/150‑8 (MQ=255)
             cacCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTaa                                                                                                                                        >  1:7735/1‑150 (MQ=255)
               ccAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAaca                                                                                                                                      >  4:1042188/1‑150 (MQ=255)
                cAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCTGGGATGTAGCGGAAATAGAGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAacac                                                                                                                                     >  3:1618134/1‑150 (MQ=255)
                 aGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACAcc                                                                                                                                    <  3:1474480/150‑1 (MQ=255)
                  gCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACAccc                                                                                                                                   >  2:899854/1‑150 (MQ=255)
                          ccGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGAt                                                                                                                           >  2:2182801/1‑150 (MQ=255)
                               aaTAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGa                                                                                                                      <  1:2331164/150‑1 (MQ=255)
                               aaTAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGa                                                                                                                      <  2:1746298/150‑1 (MQ=255)
                               aaTAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGa                                                                                                                      >  1:1746298/1‑150 (MQ=255)
                                 tAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCGTTAACACCCTCAAAGATCGCGATg                                                                                                                    >  1:1328822/1‑150 (MQ=255)
                                  aaCCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATgg                                                                                                                   >  2:2020364/1‑150 (MQ=255)
                                      gAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTaaa                                                                                                               >  2:2290933/1‑150 (MQ=255)
                                            gAAATCAGGGCGCTGCAATTTTCTTCCGGGGATGTAGCGGAAATAGAGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTgg                                                                                                         >  3:1070751/1‑150 (MQ=255)
                                            gAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTgg                                                                                                         <  3:1233525/150‑1 (MQ=255)
                                             aaaTCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACTATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTggg                                                                                                        <  1:636105/150‑1 (MQ=255)
                                               aTCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGa                                                                                                      <  2:1131139/150‑1 (MQ=255)
                                                    ggCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTCAACGGTGGGGAAACCt                                                                                                 >  2:1872532/1‑150 (MQ=255)
                                                    ggCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCt                                                                                                 <  3:17018/150‑1 (MQ=255)
                                                         tGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCa                                                                                                                                <  2:742287/114‑1 (MQ=255)
                                                                 tCTGCCGGGGATGTAGCGGAAATAGAGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGAtatttat                                                                                    <  1:2401197/150‑1 (MQ=255)
                                                                  cTGCCGGGGATGTAGCGGAAATAGAGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGAtatttatt                                                                                   <  2:1652684/150‑1 (MQ=255)
                                                                  cTGCCGGGGATGTAGCGGAAATAGAGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGAGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGAtatttatt                                                                                   <  4:1214079/150‑1 (MQ=255)
                                                                    gCCGGGGATGTAGCGGAAATAGAGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTgc                                                                                 >  1:1606854/1‑150 (MQ=255)
                                                                     ccGGGGATGTAGCGGAAATAGAGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGTGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCGGCCGGATATTTATTgcg                                                                                >  2:1721469/1‑150 (MQ=255)
                                                                       ggggATGTAGCGGAAATAGAGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGct                                                                              >  2:1564151/1‑150 (MQ=255)
                                                                           aTGTAGCGGAAATAGAGCAGACGATTCTCTCTTTGGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGcttatc                                                                          <  2:1708908/150‑1 (MQ=255)
                                                                              tAGCGGAAATAGAGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGcttatctta                                                                       <  4:832438/150‑1 (MQ=255)
                                                                              tAGCGGAAATAGAGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGcttatctta                                                                       >  1:2280374/1‑150 (MQ=255)
                                                                                gCGGAAATAGAGCAGACGATTCTTTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCttatcttatc                                                                     <  2:2428599/150‑1 (MQ=255)
                                                                                gCGGAAATAGAGCAGACGATTCTTTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCttatcttatc                                                                     >  1:2428599/1‑150 (MQ=255)
                                                                                gCGGAAATAGAGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCttatcttatc                                                                     <  2:185268/150‑1 (MQ=255)
                                                                                 cGGAAATAGAGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTTGGTGGCTCCAGTAACACTCTCAAAGATCGCGATGGTAAACGGGGGGGAAGCCTGCCGGATATTTATTGCGCTTATCTTATcc                                                                    >  1:2224534/1‑150 (MQ=255)
                                                                                 cGGAAATAGAGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATcc                                                                    <  1:2182801/150‑1 (MQ=255)
                                                                                  ggAAATAGAGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCg                                                                   >  1:1143052/1‑150 (MQ=255)
                                                                                          agCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTgg                                                           <  3:383449/150‑1 (MQ=255)
                                                                                           gCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGAtggtggtgg                                                                                                                                                     >  3:922460/1‑59 (MQ=255)
                                                                                              gACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACAcc                                                                                                                                    <  2:600127/73‑1 (MQ=255)
                                                                                              gACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACAcc                                                                                                                                    >  1:600127/1‑73 (MQ=255)
                                                                                              gACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGGGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGtttt                                                       <  4:1618134/150‑1 (MQ=255)
                                                                                                 gATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTggg                                                                                                                                                    >  2:1369789/1‑54 (MQ=255)
                                                                                                 gATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTggg                                                                                                                                                    <  1:1369789/54‑1 (MQ=255)
                                                                                                 gATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTcg                                                    <  1:2020364/150‑1 (MQ=255)
                                                                                                  aTTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTcgc                                                   <  2:1226524/150‑1 (MQ=255)
                                                                                                  aTTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTcgc                                                   <  2:1464175/150‑1 (MQ=255)
                                                                                                   ttCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTcgcg                                                  >  4:1464130/1‑150 (MQ=255)
                                                                                                   ggctctctTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTcgcg                                                  <  3:233269/148‑1 (MQ=255)
                                                                                                    tctctcTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCgg                                                 <  3:1049488/150‑1 (MQ=255)
                                                                                                    tctctcTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGCGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCgg                                                 >  2:1785695/1‑150 (MQ=255)
                                                                                                     ctctctTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCggg                                                >  3:99327/1‑150 (MQ=255)
                                                                                                          ttttGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAAtt                                           >  1:1629790/1‑150 (MQ=255)
                                                                                                              gTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTg                                       <  4:1265918/150‑1 (MQ=255)
                                                                                                               tCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTgg                                      <  1:1809594/150‑1 (MQ=255)
                                                                                                               tCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTgg                                      >  1:1569220/1‑150 (MQ=255)
                                                                                                               tCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTgg                                      <  2:88649/150‑1 (MQ=255)
                                                                                                               tCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTgg                                      <  2:879192/150‑1 (MQ=255)
                                                                                                                  aTGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGata                                                                                         >  1:624001/1‑96 (MQ=255)
                                                                                                                       tgccACACCCGTAAAGTGGCGATGGTGGTGGGTAGCGCCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGc                              <  1:199154/150‑1 (MQ=255)
                                                                                                                        gccACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCg                             >  4:1184116/1‑150 (MQ=255)
                                                                                                                        gccACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTCTTCGCGGGGAATTAGTGGGATACGGCg                             >  2:628838/1‑150 (MQ=255)
                                                                                                                         ccACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGa                            >  3:1357807/1‑150 (MQ=255)
                                                                                                                          cacaCCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGAtatt                                                                                       >  2:1299822/1‑90 (MQ=255)
                                                                                                                          cacaCCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGAtatt                                                                                       <  1:1299822/90‑1 (MQ=255)
                                                                                                                           acacCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGAtt                          <  2:1201554/150‑1 (MQ=255)
                                                                                                                               ccGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGtt                      >  1:2631739/1‑150 (MQ=255)
                                                                                                                               ccGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGtt                      >  4:358892/1‑150 (MQ=255)
                                                                                                                               ccGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGtt                      >  4:1051673/1‑150 (MQ=255)
                                                                                                                                 gTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTc                    >  1:1507152/1‑150 (MQ=255)
                                                                                                                                   aaaGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGt                  <  1:522695/150‑1 (MQ=255)
                                                                                                                                    aaGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTa                 >  2:1460755/1‑150 (MQ=255)
                                                                                                                                     aGTGGCGATGGTGGTGGGTAGCTCCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTAt                <  1:832579/150‑1 (MQ=255)
                                                                                                                                      gTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATgg                                                                                                                   >  1:911384/1‑50 (MQ=255)
                                                                                                                                      gTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATg               >  3:263653/1‑150 (MQ=255)
                                                                                                                                      gTGGCGATGGTGGTGGGTAGCACCATTAACACCCCCAAAGATCGCGATGGTAAACGGCGGGGAAACCTGCCGGATATTTAGTGCGCTTCTCTTAACCGACGTCTGGCTTTTCGCGTGGGATTAGTGGGCTACGGCGATTTGATTCGTATg               >  4:1554544/1‑150 (MQ=255)
                                                                                                                                         gCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATgcgc            >  4:520131/1‑150 (MQ=255)
                                                                                                                                          cGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCt           <  4:1070751/150‑1 (MQ=255)
                                                                                                                                          cGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCt           <  1:1026064/150‑1 (MQ=255)
                                                                                                                                           gATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAAGCTGCGGGATATTTTTTGGGCTTAGCTTAGGCGGTGGCTGGTTTTTCGCGGGGAAGTAGTGGGATACGGCGATTTGTTTCGTATgcgcgt          >  1:1562134/1‑148 (MQ=255)
                                                                                                                                                 ggtggGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGa    >  1:2014802/1‑150 (MQ=255)
                                                                                                                                                  gtggGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGt                  >  1:1014112/1‑135 (MQ=255)
                                                                                                                                                  ccaggTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGaa   >  2:2116645/4‑150 (MQ=255)
                                                                                                                                                   caggTAGCCCCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGaaa  <  1:1430214/148‑1 (MQ=255)
                                                                                                                                                   caggTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTg                                       <  2:169493/111‑1 (MQ=255)
                                                                                                                                                   caggTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTg                                       >  1:169493/3‑113 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
ATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAA  >  NC_000913_3_KO_pgi/4383822‑4384118

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.