| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| JC | NC_000913_3_KO_pgi | 4,233,757 | +ATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTAC | intergenic (‑524/‑583) | lysC ← / → yjbE | lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein |
| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | NC_000913_3_KO_pgi | = 4233757 | 0 (0.000) | 23 (0.880) +ATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTAC |
21/188 | 0.3 | 100% | intergenic (‑524/‑583) | lysC/yjbE | lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein |
| ? | NC_000913_3_KO_pgi | 4233758 = | 0 (0.000) | intergenic (‑525/‑582) | lysC/yjbE | lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein | |||||
CGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCA > NC_000913_3_KO_pgi/4233610‑4233889 | cGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTAGAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAa < 1:544973‑M1/149‑2 (MQ=255) ggTAAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAat < 2:575609‑M1/149‑3 (MQ=255) cTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCTCTTCAGGTATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatc < 1:335480‑M1/149‑17 (MQ=255) gCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcaga > 1:593879‑M1/1‑124 (MQ=255) caagGGCTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCGCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggc > 2:633883‑M1/2‑112 (MQ=255) cTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcactccaccgcagaccgctgcctgccaggcactacggt > 1:522725‑M1/1‑100 (MQ=255) aaCATTACGCTAACGGCACGAAAACCATCACATTTTTCGGGGACTGGCGCTACAATCGTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgta < 2:340440‑M1/149‑53 (MQ=255) aCAATACGCAAACGGCACTAAAACCATCACATTTTTCTGCGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCCGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagcccgctgcctggcaggcactacggtgtag < 2:391320‑M1/149‑54 (MQ=255) aTTACGCTAACCGCAACACAAACACCACATTATTCTCTGACTGCCGCTACAAGCTTCCAAAGTCACAATTCTCAAAAGCACAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggc < 2:284678‑M1/149‑56 (MQ=255) aCGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctgg < 1:537237‑M1/149‑59 (MQ=255) gCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggcg > 2:2671‑M1/1‑89 (MQ=255) gCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttc < 1:452112‑M1/149‑68 (MQ=255) cTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaa > 1:587095‑M1/1‑79 (MQ=255) tCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAGGTCACAAGTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctata < 1:16640‑M1/149‑80 (MQ=255) tttttCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaggttcctatactttc > 1:89610‑M1/1‑65 (MQ=255) tttttCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttc < 1:187873‑M1/149‑85 (MQ=255) tgtgACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgctt > 2:267920‑M1/1‑59 (MQ=255) tgtgACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgctt < 1:267920‑M1/125‑67 (MQ=255) cAATCTTCCAAAGTCACAATTCTCAACATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcattacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaa < 1:463719‑M1/149‑105 (MQ=255) cAATCTTCCAAAGTCAAAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaa < 2:503751‑M1/149‑105 (MQ=255) ttCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgca > 1:640513‑M1/1‑40 (MQ=255) tcgcaaaTTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgca < 2:173263‑M1/146‑110 (MQ=255) cAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggt < 2:593879‑M1/149‑113 (MQ=255) gTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgac < 1:41632‑M1/149‑117 (MQ=255) tCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacg < 2:668520‑M1/149‑118 (MQ=255) aaaaTCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaa > 2:472832‑M1/1‑21 (MQ=255) aTCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctaactgtcatgcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgc < 1:477229‑M1/149‑132 (MQ=255) tCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgct > 1:260814‑M1/1‑17 (MQ=255) | CGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCA > NC_000913_3_KO_pgi/4233610‑4233889 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |