Predicted mutation
evidence seq id position mutation annotation gene description
JC NC_000913_3_KO_pgi 4,233,757 +ATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTAC intergenic (‑524/‑583) lysC ← / → yjbE lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_KO_pgi = 42337570 (0.000)23 (0.880)
+ATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTAC
21/188 0.3 100% intergenic (‑524/‑583) lysC/yjbE lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein
?NC_000913_3_KO_pgi 4233758 = 0 (0.000)intergenic (‑525/‑582) lysC/yjbE lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein

GTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGC  >  NC_000913_3_KO_pgi/4233612‑4233904
                                                                                                                                                  |                                                                                                                                                  
ctcaacattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcactccaccgcagaccgctgcctgccaggcactacGgt                                                                                                                                                  >  1:522725‑M2/147‑149 (MQ=255)
   aacattacgctaacggcacgaaaaccatcacatttttcggggactggcgctacaatcgtccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTa                                                                                                                                               <  2:340440‑M2/6‑1 (MQ=255)
    acaatacgcaaacggcactaaaaccatcacatttttctgcgactggcgctacaatcttccaaagtcacaattctcaaaatccgaagagtattgctaatgaaaaacatcaatccaacgcagcccgctgcctggcaggcactacGGTGTAg                                                                                                                                              <  2:391320‑M2/7‑1 (MQ=255)
      attacgctaaccgcaacacaaacaccacattattctctgactgccgctacaagcttccaaagtcacaattctcaaaagcacaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGc                                                                                                                                            <  2:284678‑M2/9‑1 (MQ=255)
         acgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTgg                                                                                                                                         <  1:537237‑M2/12‑1 (MQ=255)
           gctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGctggcg                                                                                                                                       >  2:2671‑M2/136‑147 (MQ=255)
                  gcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTc                                                                                                                                <  1:452112‑M2/21‑1 (MQ=255)
                     ctaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGaa                                                                                                                             >  1:587095‑M2/126‑149 (MQ=255)
                              tcacatttttctgtgactggcgctacaatcttccaaggtcacaagtctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCtata                                                                                                                    <  1:16640‑M2/33‑1 (MQ=255)
                                   tttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAGGTTCCTATACTTTc                                                                                                               >  1:89610‑M2/112‑149 (MQ=255)
                                   tttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTc                                                                                                               <  1:187873‑M2/38‑1 (MQ=255)
                                         tgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCtt                                                                                                                                 <  1:267920‑M2/20‑1 (MQ=255)
                                         tgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCtt                                                                                                                                 >  2:267920‑M2/106‑125 (MQ=255)
                                                       caatcttccaaagtcacaattctcaacatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcattacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGaa                                                                                           <  1:463719‑M2/58‑1 (MQ=255)
                                                       caatcttccaaagtcaaaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGaa                                                                                           <  2:503751‑M2/58‑1 (MQ=255)
                                                            ttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCa                                                                                      >  1:640513‑M2/87‑149 (MQ=255)
                                                            tcgcaaattcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCa                                                                                      <  2:173263‑M2/63‑1 (MQ=255)
                                                               caaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGt                                                                                   <  2:593879‑M2/66‑1 (MQ=255)
                                                                   gtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGAc                                                                               <  1:41632‑M2/70‑1 (MQ=255)
                                                                    tcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACg                                                                              <  2:668520‑M2/71‑1 (MQ=255)
                                                                               aaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGaa                                                                   >  2:472832‑M2/68‑149 (MQ=255)
                                                                                  atcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctaactgtcatgcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGgaatgc                                                                <  1:477229‑M2/85‑3 (MQ=255)
                                                                                   tcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCggaatgct                                                               >  1:260814‑M2/64‑146 (MQ=255)
                                                                                                                  tccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATcccc                                                                       >  2:184439‑M2/33‑110 (MQ=255)
                                                                                                                  tccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATcccc                                                                       <  1:184439‑M2/78‑1 (MQ=255)
                                                                                                                                           gcactacggtgtaggcTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgata       >  1:124570‑M1/8‑90 (MQ=255)
                                                                                                                                           gcactacggtgtaggcTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgata       >  1:397771‑M1/8‑90 (MQ=255)
                                                                                                                                                acggtgTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtag  <  1:98076‑M1/147‑65 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGC  >  NC_000913_3_KO_pgi/4233612‑4233904

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.