Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913_3_KO_pgi 1,299,499 Δ1,199 bp intergenic (+254/‑485) ychE → / → oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913_3_KO_pgi 1299499–1300697 1300697 1–1199 29 [0] [0] 29 ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_KO_pgi = 12994980 (0.000)25 (0.630) 23/274 0.9 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913_3_KO_pgi 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

TAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCCCG  >  NC_000913_3_KO_pgi/1299372‑1299646
                                                                                                                              |                                                                                                                                                    
taAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaatta                                                                                                                                >  1:422920‑M1/1‑127 (MQ=255)
          cTTAACTGCTTCTCCTCTCCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcattt                                                                                                                      <  1:258224‑M1/149‑33 (MQ=255)
                   ttCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactgg                                                                                                             >  2:324240‑M1/1‑108 (MQ=255)
                        ctcACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaatttttaaattattgtgcatttcactactggaactg                                                                                                        >  1:589092‑M1/1‑103 (MQ=255)
                                         ttCGCCACATCATAATCCTGGGCTTGCTGAAGAATAAGTGAAATGATATTCTTAATTCCACTGCATTTGGTAGACGAAAGTGCTAAataataatcaattgttaaattattgtgcatttaactactgtaactctaatcagaaaacatgca                                                                                       >  2:387682‑M1/1‑86 (MQ=255)
                                          tCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAatcataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagac                                                                                      <  1:3415‑M1/149‑65 (MQ=255)
                                             ccACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaatt                                                                                                                                         >  1:651913‑M1/1‑82 (MQ=255)
                                             ccACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaatt                                                                                                                                         <  2:651913‑M1/95‑14 (MQ=255)
                                                   catAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGCAAGTGATCAataataatcaatcgttaaattatggtacattgcactactcgaactgtaatcagaaaagatagacatgcttagc                                                                             >  2:605875‑M1/1‑76 (MQ=255)
                                                   catAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaactattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagc                                                                             <  1:476648‑M1/149‑74 (MQ=255)
                                                              gCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactg                                                                                                              <  2:266100‑M1/105‑41 (MQ=255)
                                                              gCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactg                                                                                                              >  1:266100‑M1/1‑65 (MQ=255)
                                                                ttGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggcactgtaatcagaaaagatagacatgcttaggcaatctctatttg                                                                >  1:547757‑M1/1‑63 (MQ=255)
                                                                    tGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGCAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatatgattg                                                            >  1:506935‑M1/1‑59 (MQ=255)
                                                                                   aTGATATTATTAATTCCACTTCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgttt                                             <  2:199405‑M1/149‑106 (MQ=255)
                                                                                        attattAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaa                                        <  1:590490‑M1/149‑111 (MQ=255)
                                                                                                ttCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatgga                                >  1:177222‑M1/1‑31 (MQ=255)
                                                                                                      tGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaag                          <  1:620378‑M1/149‑125 (MQ=255)
                                                                                                        ccTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagct                        >  2:379915‑M1/1‑23 (MQ=255)
                                                                                                          tttGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtagtcagaaaagatagacatgcttagccaatctctatttgatcgaattgaaagatgtttgttaaggcatggatgcaagctat                      >  2:592079‑M1/1‑21 (MQ=255)
                                                                                                          tttGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctctttaattgaattgaaagatcttagttacggcatggatgcaagctat                      >  2:631138‑M1/1‑21 (MQ=255)
                                                                                                                 gagGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagc                                                                             <  1:617606‑M1/87‑74 (MQ=255)
                                                                                                                 gagGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagc                                                                             >  2:617606‑M1/1‑14 (MQ=255)
                                                                                                                   ggAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctga             <  1:589035‑M1/149‑138 (MQ=255)
                                                                                                                     aaaGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgata           <  1:52584‑M1/149‑140 (MQ=255)
                                                                                                                           cTAAataataatcaattgttaaattattgtgcatttcactactggaactttaatcagaaaagatagacatgcttagccaatctctatttgattgacttgaaagatgtttgttaaggcatggatgcaagctatagattctgatacggtta     >  1:427259‑M1/1‑4 (MQ=255)
                                                                                                                           cTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgatacggtca     >  1:237064‑M1/1‑4 (MQ=255)
                                                                                                                            tAAataataatcaattgttaaattattgttcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgatacggtaaa    >  2:84517‑M1/1‑3 (MQ=255)
                                                                                                                              aataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgatacggtcaata  >  1:470268‑M1/1‑1 (MQ=255)
                                                                                                                              |                                                                                                                                                    
TAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCCCG  >  NC_000913_3_KO_pgi/1299372‑1299646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.