Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913_3_KO_pgi 1,299,499 Δ1,199 bp intergenic (+254/‑485) ychE → / → oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913_3_KO_pgi 1299499–1300697 1300697 1–1199 29 [0] [0] 29 ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_KO_pgi = 12994980 (0.000)25 (0.630) 23/274 0.9 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913_3_KO_pgi 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

GGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAATA  >  NC_000913_3_KO_pgi/1300571‑1300845
                                                                                                                               |                                                                                                                                                   
taaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAatta                                                                                                                                >  1:422920‑M2/128‑149 (MQ=255)
          cttaactgcttctcctctccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCAttt                                                                                                                      <  1:258224‑M2/32‑1 (MQ=255)
                   ttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTgg                                                                                                             >  2:324240‑M2/109‑149 (MQ=255)
                        ctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTTTTAAATTATTGTGCATTTCACTACTGGAACTg                                                                                                        >  1:589092‑M2/104‑149 (MQ=255)
                                         ttcgccacatcataatcctgggcttgctgaagaataagtgaaatgatattcttaattccactgcatttggtagacgaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTAACTACTGTAACTCTAATCAGAAAacatgca                                                                                       >  2:387682‑M2/87‑146 (MQ=255)
                                          tcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATCATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGAc                                                                                      <  1:3415‑M2/64‑1 (MQ=255)
                                             ccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAAtt                                                                                                                                         >  1:651913‑M2/83‑95 (MQ=255)
                                             ccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAAtt                                                                                                                                         <  2:651913‑M2/13‑1 (MQ=255)
                                                   cataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggcaagtgatcaATAATAATCAATCGTTAAATTATGGTACATTGCACTACTCGAACTGTAATCAGAAAAGATAGACATGCTTAGc                                                                             >  2:605875‑M2/77‑149 (MQ=255)
                                                   cataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAACTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGc                                                                             <  1:476648‑M2/73‑1 (MQ=255)
                                                              gcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTg                                                                                                              <  2:266100‑M2/40‑1 (MQ=255)
                                                              gcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTg                                                                                                              >  1:266100‑M2/66‑105 (MQ=255)
                                                                ttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGCACTGTAATCAGAAAAGATAGACATGCTTAGGCAATCTCTATttg                                                                >  1:547757‑M2/64‑149 (MQ=255)
                                                                    tgaagaataattgaaatgatattattaattccactgcctttggcagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATatgattg                                                            >  1:506935‑M2/60‑149 (MQ=255)
                                                                                   atgatattattaattccacttcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgttt                                             <  2:199405‑M2/105‑1 (MQ=255)
                                                                                        attattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTaa                                        <  1:590490‑M2/110‑1 (MQ=255)
                                                                                                ttccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGa                                >  1:177222‑M2/32‑149 (MQ=255)
                                                                                                      tgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAg                          <  1:620378‑M2/124‑1 (MQ=255)
                                                                                                        cctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCt                        >  2:379915‑M2/24‑149 (MQ=255)
                                                                                                          tttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAGTCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATCGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCtat                      >  2:592079‑M2/22‑149 (MQ=255)
                                                                                                          tttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTCTTTAATTGAATTGAAAGATCTTAGTTACGGCATGGATGCAAGCtat                      >  2:631138‑M2/22‑149 (MQ=255)
                                                                                                                 gaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGc                                                                             <  1:617606‑M2/73‑1 (MQ=255)
                                                                                                                 gaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGc                                                                             >  2:617606‑M2/15‑87 (MQ=255)
                                                                                                                   ggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGa             <  1:589035‑M2/137‑1 (MQ=255)
                                                                                                                     aaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATa           <  1:52584‑M2/139‑1 (MQ=255)
                                                                                                                           ctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTTTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGACTTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACggtta     >  1:427259‑M2/5‑147 (MQ=255)
                                                                                                                           ctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCa     >  1:237064‑M2/5‑149 (MQ=255)
                                                                                                                            taaATAATAATCAATTGTTAAATTATTGTTCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTaaa    >  2:84517‑M2/4‑149 (MQ=255)
                                                                                                                              aATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAATa  >  1:470268‑M2/2‑149 (MQ=255)
                                                                                                                               |                                                                                                                                                   
GGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAATA  >  NC_000913_3_KO_pgi/1300571‑1300845

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.