New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_KO_pgi = 266239260 (1.180)7 (0.210)
+CTACAGTGAACAGAACCGTAGGTCGGATAAGGCGTTCACGCCGCATCCG		 4/188 5.2 15.1% noncoding (84/266 nt) REP184 (repetitive extragenic palindromic) element; contains 6 REP sequences REP184 (repetitive extragenic palindromic) element; contains 6 REP sequences
?NC_000913_3_KO_pgi = 2662583 NA (NA)coding (741/741 nt) trmJ tRNA mC32,mU32 2'‑O‑methyltransferase, SAM‑dependent
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCA‑TTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCACGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAA  >  NC_000913_3_KO_pgi/2662441‑2662729
                                                                                                                                               |                                                                                                                                                  
ttCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCAGATGCGGCGGGAACCCCTTATCCGACCTACGATTCTGGTCACTGTAGGCCGGAGGAGACGCA‑TCACCCGCCTCATCCGCCGACGGCTGTTaacca                                                                                                                                              >  2:645523/1‑145 (MQ=11)
 tCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCA‑TTACGCGTCGCATCAGGCAACGGCTGTTATTCgg                                                                                                                                             <  1:745156/149‑1 (MQ=35)
     tGTAGGCCTGATAAGACGCATTACGCGTCACAACAAGGAGCTGCTGTCTGATGCGCCGTGAACGACTTATCCGACCTACGGGTCTGTTCACTGTATGCCCCATGAGA‑GCACTTACGCGCCGCATCATGCGCCGGCGGTTATTCTGGCtt                                                                                                                                         >  2:874509/1‑149 (MQ=2)
       tAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCA‑TTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGt                                                                                                                                       <  2:880189/149‑1 (MQ=40)
          gCCTGATAAGGCGCATTCCCCGTCGCATCCGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCA‑TTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTAc                                                                                                                                    <  1:49105/149‑1 (MQ=255)
                 aaGACGCGTTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGTCCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCA‑TTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTAtt                                                                                                                             <  1:353013/149‑1 (MQ=255)
                    ccgttttACGCGGCGCGGCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGCCCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCA‑TTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTctg                                                                                                                          <  2:380912/144‑1 (MQ=17)
                       cATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCA‑TTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTctactg                                                                                                                       <  1:863938/149‑1 (MQ=255)
                         ttACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCA‑TTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTgctgct                                                                                                                     >  1:570280/1‑149 (MQ=255)
                              ggTCGCATAAGGCAACGGCTGTCGGATGCGGCGTTAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCA‑TTATGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATa                                                                                                                <  2:720078/148‑1 (MQ=255)
                               gTCGCATCAGGAAACGGCTGTCGGATGCGGCGTGAACGCCTTCTTCGACCTACGGTTCTGTTAACTGTGGGCCTGATAAGCAGCA‑TTACGCGTCGGATATGGCGACGGCTGTTCTTCGGCCTTGTTAGCTTTAATGTGATGCCCAATac                                                                                                               >  2:732502/1‑148 (MQ=14)
                                 cGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCA‑TTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGaa                                                                                                             <  1:89517/149‑1 (MQ=255)
                                    aTCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCA‑TTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGATCAATAGCAGcc                                                                                                          >  2:91807/1‑149 (MQ=255)
                                     tCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCA‑TTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCa                                                                                                         <  1:16494/149‑1 (MQ=255)
                                       aGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCA‑TTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGa                                                                                                       >  1:857109/1‑149 (MQ=255)
                                         gCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCA‑TTACGCGTCGCATCAGTCAACGGCTGTTATTCGCGCTTGGTACCCTTATTCCGCTGCACAATAGGAGTCACAAt                                                                                                     >  2:289968/1‑149 (MQ=14)
                                           aaCGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGAGGCA‑TTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCCATAGAAGCCAGAAtgc                                                                                                   >  2:744086/1‑147 (MQ=255)
                                                      gATGCGGCGGTAACGCCTGATCGGACCTCTGGTACTGTTCACTGTAGGCCTGATAAGACGCA‑TTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGata                                                                                        <  2:125883/149‑1 (MQ=25)
                                                        tGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCA‑TTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATAtt                                                                                      <  2:13699/149‑1 (MQ=255)
                                                         gcggctTGAACCCCTCATCCGGCCTACGGTTTCGTTCACTGTAGGCCTGATAAGACGCA‑TTACGCGCCTCATCACGCAACTGCTGTTATTCGGCCTTGTTACCTTTTTTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTc                                                                                     <  2:145835/149‑1 (MQ=25)
                                                           ggcgTGAAGGGCTTATCCGGCCTAGGGTTCTGTTCGCTGTAGGCCTGATAAGACGCA‑TTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCaa                                                                                   <  2:86726/149‑1 (MQ=25)
                                                            ccgTGAATGCCTTGGCCGACCTACGGTTCTATTCACTGTAGGCCTGATAAGACGCA‑TTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCCCAATAGAAGCCAGAATCCCGCCCCGGATCTTCAAc                                                                                  >  2:689214/2‑149 (MQ=21)
                                                                   cGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCA‑TTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTa                                                                                                                               <  2:900366/97‑1 (MQ=255)
                                                                   cGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCA‑TTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTa                                                                                                                               >  1:900366/1‑97 (MQ=255)
                                                                      cTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCA‑TTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCttt                                                                        <  1:289968/149‑1 (MQ=255)
                                                                          tCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCA‑TTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCgg                                                                    >  1:431755/1‑149 (MQ=255)
                                                                             gACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCA‑TTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGgcg                                                                 >  1:41827/1‑149 (MQ=255)
                                                                                 tACGGTTCTGTTCACTGTAGGCCTGATAAGACGCA‑TTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCaa                                                                                   >  2:718203/1‑127 (MQ=255)
                                                                                 tACGGTTCTGTTCACTGTAGGCCTGATAAGACGCA‑TTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCaa                                                                                   <  1:718203/127‑1 (MQ=255)
                                                                                      ttatGTCCACTGTAGGCTTGATAAGACGCA‑TTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAACCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCACGGGt                                                        <  1:502217/146‑1 (MQ=255)
                                                                                          gTTCACTGTAGGCCTGATAAGACGCA‑TTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCACGGGTAAAc                                                    >  1:627910/1‑149 (MQ=255)
                                                                                           gtCAATGTAGGCCTGATAAGACGCA‑TTACGCGACGCATCAGACAAAAACGAAAATTCGGCCTTGTTACCTTTATTCTGCGGCTCAATAGAAGCCTGAATACCGCGCAGGATATTCAACTCCTGGCTTTCCAGACGCGCAAGGGTAAACa                                                   >  2:685143/2‑149 (MQ=25)
                                                                                                 gTAGGCCTGATAAGACGCA‑TTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCACGGGTAAACAGACGgc                                             <  2:145535/149‑1 (MQ=255)
                                                                                                 agagtccTGATAAGACGCA‑TTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCACGGGTAAACAGACGgc                                             <  2:447153/144‑1 (MQ=255)
                                                                                                  tAGGCCTGATAAGACGCA‑TTACGCGTCGCATTAGGCAACGGCTTTTATTCGGCCTTGTTACCTTTATTCTGCTGCCCAATAGAAGCCACAATCCCGCCCAGAATCTTAAACTCCTTGCGTTCCTGGCCCGCACAGGGAACCAGCCGgcg                                            >  2:120710/1‑149 (MQ=25)
                                                                                                    ggCCTGATAAGACGCA‑TTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCACGGGTAGACAGACGGCGCa                                          >  1:652521/1‑149 (MQ=255)
                                                                                                       cTGATAAGACGCA‑TTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCACGGGTAAACAGACGGCGCAAtt                                       >  1:103056/1‑149 (MQ=255)
                                                                                                         gATAAGACGCA‑TTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCACGGGTAAACAGACGGCGCAATTTa                                     <  1:50272/149‑1 (MQ=255)
                                                                                                           tAAGACGCA‑TTACGCGTAGTATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCACGGGTAAACAGACGGCGCAATTTAtt                                   <  2:166839/149‑1 (MQ=255)
                                                                                                              gACGCA‑TTACGCGTCGCATCAGGCAAAGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCACGGGTAAACAGACTGGGCAATATATtcat                                >  2:240155/1‑149 (MQ=255)
                                                                                                               aCGCA‑TTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAACCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCACGGGTCAACAGAAGGCGCAATTTATtcatc                               >  1:905658/1‑149 (MQ=255)
                                                                                                                            tCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCACGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGa                   <  2:368717/149‑1 (MQ=255)
                                                                                                                            tCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCACGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGa                   <  1:789378/149‑1 (MQ=255)
                                                                                                                              gCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCACGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATg                 <  2:648962/149‑1 (MQ=255)
                                                                                                                                   aGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCACGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGtttt            <  1:54171/149‑1 (MQ=255)
                                                                                                                                      cAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGCTATTCAACTCCGGGCTTTCCGGGCGCGCACGGGTAAACAGACGGCGCAATTTATTAATCGCCTGCCCCGGGGGGTTTTCAc         >  1:730291/1‑149 (MQ=255)
                                                                                                                                       aaCGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCACGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACg        <  1:647077/149‑1 (MQ=255)
                                                                                                                                        aCGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCACGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACgg       <  1:580953/149‑1 (MQ=255)
                                                                                                                                          ggCTGTTGTTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCCGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGTGCGCGCACGGGTAAACAGACGGCGCACTTTAGTCTACACGTGCCCGGATTAGTTTGCACgcgc     >  2:236920/1‑146 (MQ=255)
                                                                                                                                             tGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCACGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTACACGGATaaa  >  1:798047/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                  
TTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCA‑TTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCACGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAA  >  NC_000913_3_KO_pgi/2662441‑2662729

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.