Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913_3_KO_pgi	1,299,499	Δ1,199 bp	intergenic (+254/‑485)	ychE → / → oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913_3_KO_pgi	1299499–1300695	1300697	3–1199	57 [0]	[0] 57	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_KO_pgi	= 1299498	0 (0.000)	56 (1.130)	46/278	0.1	100%	intergenic (+253/‑1684)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
	?	NC_000913_3_KO_pgi	1300698 =	0 (0.000)					intergenic (+1453/‑484)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

CTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCC  >  NC_000913_3_KO_pgi/1299353‑1299644                                                                                                                                                  |                                                                                                                                                   ctcAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAata                                                                                                                                                 >  2:283749‑M1/1‑146 (MQ=255)  tcAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataa                                                                                                                                                >  1:123776‑M1/1‑145 (MQ=255)    aaaTTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataata                                                                                                                                              <  1:703950‑M1/149‑7 (MQ=255)     aaTTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGATGAAGGATAGGGGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataa                                                                                                                                             >  1:433813‑M1/1‑142 (MQ=255)          cAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaat                                                                                                                                        >  2:905468‑M1/1‑137 (MQ=255)             ttagttaTAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcatttgt                                                                                                                                     >  1:135136‑M1/1‑134 (MQ=255)                     aaGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGATCAataataaccactttttaatcaat                                                                                                                             >  2:398966‑M1/1‑126 (MQ=255)                         aTTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATGATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTACTACaaaataatcaactgttaaattattgtg                                                                                                                         >  2:657965‑M1/1‑122 (MQ=255)                              cTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcattt                                                                                                                    >  1:853542‑M1/1‑117 (MQ=255)                                   cTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGTAAAGTGCTAAataataatcaatttttaatttattgtacatttcccta                                                                                                               >  2:426786‑M1/1‑112 (MQ=255)                                   cTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcacta                                                                                                               <  1:738509‑M1/149‑38 (MQ=255)                                     gCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactact                                                                                                             <  1:283749‑M1/149‑40 (MQ=255)                                       ttCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGGGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcaatactgg                                                                                                           >  1:597381‑M1/1‑108 (MQ=255)                                       ttCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactgg                                                                                                           >  1:515685‑M1/1‑108 (MQ=255)                                          tcctcACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaac                                                                                                        <  2:123776‑M1/149‑45 (MQ=255)                                            ctcACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaacag                                                                                                      >  1:184359‑M1/1‑103 (MQ=255)                                             tcACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgt                                                                                                     <  2:322775‑M1/149‑48 (MQ=255)                                               aCCATCCTGTTATTTTCGCCACATCATAACCCTGGGCTTTCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTTGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaa                                                                                                   <  2:480982‑M1/149‑50 (MQ=255)                                               aCCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattatttttcatttcattactggaactttaa                                                                                                   >  1:32952‑M1/1‑100 (MQ=255)                                                      tGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattattcaattattgtgcatttcactactggaactgtaatcaggaa                                                                                            >  1:165428‑M1/1‑93 (MQ=255)                                                       gTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaa                                                                                           <  1:272570‑M1/149‑58 (MQ=255)                                                       gTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcacggcttgaactgtaatcagaaaa                                                                                           <  2:878697‑M1/149‑58 (MQ=255)                                                        ttATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaag                                                                                          >  2:573221‑M1/1‑91 (MQ=255)                                                             ttCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagataga                                                                                     <  1:759506‑M1/149‑64 (MQ=255)                                                                        atAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagcc                                                                          >  1:140616‑M1/1‑75 (MQ=255)                                                                            tCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatc                                                                      >  2:179575‑M1/1‑71 (MQ=255)                                                                               ttggctTGCTGAAGAATTAGTGAAATTATATTATTAATTCCACTGCCTTTGGTAGAGGAAATTGCTAAtttataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagcctttcttagccaatctct                                                                   <  2:335002‑M1/147‑82 (MQ=255)                                                                               tGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctct                                                                   <  2:335004‑M1/149‑82 (MQ=255)                                                                                        tGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgctta                                                                             >  2:716206‑M1/1‑59 (MQ=255)                                                                                        tGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgctta                                                                             <  1:716327‑M1/130‑72 (MQ=255)                                                                                        tGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgctta                                                                             <  1:716206‑M1/130‑72 (MQ=255)                                                                                        tGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgctta                                                                             >  2:716327‑M1/1‑59 (MQ=255)                                                                                        tGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGAGAGAGGAAAGTGCTGCaagagaataaattgttaaatcattatgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatatctatttgcttg                                                          >  2:871641‑M1/1‑59 (MQ=255)                                                                                                aatTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaa                                                  <  2:433813‑M1/149‑99 (MQ=255)                                                                                                  ttGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagc                                                                           >  1:745283‑M1/1‑49 (MQ=255)                                                                                                  ttGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagc                                                                           <  2:745283‑M1/122‑74 (MQ=255)                                                                                                      aaTGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagaaatgcttagccaatctctattttattgaatagaaagatgtt                                            >  2:220363‑M1/1‑45 (MQ=255)                                                                                                       aTGATATTATTAATTCCACTGGCGTTTGGAGAGGAAAGTGTGAAataataatcaatttttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgttt                                           <  2:368910‑M1/149‑106 (MQ=255)                                                                                                           tattatTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgtttagccaatctctatttgattgaattgaaaggtgtttgtta                                       >  2:765936‑M1/1‑40 (MQ=255)                                                                                                                  aaTTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccactataaacgccggttaattgaaagatgtttgttaaggcatg                                <  1:670435‑M1/149‑117 (MQ=255)                                                                                                                     tCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaagtgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggat                             <  1:271114‑M1/149‑120 (MQ=255)                                                                                                                      cccctggCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactgcaactgtaatcagaaaagatagacaggcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatg                            <  1:372472‑M1/146‑121 (MQ=255)                                                                                                                        aCTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaaaatgtttgttaagccatgaatgca                          >  2:15171‑M1/1‑27 (MQ=255)                                                                                                                        aCTGCCTTTGGTAGAGGAAAGTGATAAataatgatcaattgtgaacttattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcc                          >  1:715291‑M1/1‑27 (MQ=255)                                                                                                                            ccTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagct                      >  2:390649‑M1/1‑23 (MQ=255)                                                                                                                              tttGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctat                    <  1:482447‑M1/149‑129 (MQ=255)                                                                                                                              tttGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctat                    <  1:179575‑M1/149‑129 (MQ=255)                                                                                                                              tttGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgggcatttcactactggaactgtaatccgaatagatagacatgcttagccaatcactatttgattaaattgaaagatgtttgttaaggcatggatgcaagctat                    >  2:512425‑M1/1‑21 (MQ=255)                                                                                                                              tttGGTAGAGGAAAGTACTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctat                    >  1:788678‑M1/1‑21 (MQ=255)                                                                                                                               ttGGTAGAGGAAAGTGCTAAatactaacgccattgtgtgttattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaagacatggatgaaagctata                   >  2:420187‑M1/1‑20 (MQ=255)                                                                                                                                 ggTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctataga                 >  1:75086‑M1/1‑18 (MQ=255)                                                                                                                                 ggTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctataga                 <  1:190649‑M1/149‑132 (MQ=255)                                                                                                                                   tAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaat                                                                                                  >  2:417043‑M1/1‑16 (MQ=255)                                                                                                                                   tAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaat                                                                                                  <  1:417043‑M1/66‑51 (MQ=255)                                                                                                                                        gAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgat          >  1:145946‑M1/1‑11 (MQ=255)                                                                                                                                        gAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaagtgaaagatgtttgttaaggcatggatgcaagctatagattctgat          >  1:344610‑M1/1‑11 (MQ=255)                                                                                                                                                tAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgatacggtcaa  <  2:593979‑M1/149‑147 (MQ=255)                                                                                                                                                  |                                                                                                                                                   CTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCC  >  NC_000913_3_KO_pgi/1299353‑1299644

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑