New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_pgi 3221823 =24 (0.820)5 (0.180) 4/266 3.5 15.2% coding (358/984 nt) ebgR transcriptional repressor
?NC_000913_3_pae_pgi 3221880 = 33 (1.190)coding (415/984 nt) ebgR transcriptional repressor
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CCCAGTGCGAAAAGCTGGGCATCGAGCTCACCAACTGTTATGAACACAGCGGCTTACCAGACATTAAAAACGTCACCGGTATTTTAATTGTCGGCAAACCCACGCCCGCCCTGCGCGCCGCTGCCAGCGCGTTGACCGACAATATCTGTTTTATCGACTTTCACGAACCCGGCAGCGGTTACGATGCGGTGGATATCGATCTGGCACGCATCAGTAAAGAAATCATCGACTTCTATATCAACCAGGGCGTTAATCGTATTGGTTTTATTGGCGGTGAAGATGAGCCTG  >  NC_000913_3_pae_pgi/3221737‑3222024
                                                                                                                                               |                                                                                                                                                
ccccGTGCGAAAACCTGGGCATCGAGCTCACCAACTGTTATGAACACAGCGGCTTACCAGACATTAAAAACGTCACCGTTATTTTAATTGTCGGCAAACCCACGCCCGCCCTGCGCGCCGCTGCCAGCGCGTTGACCGACAATATCTGt                                                                                                                                             <  1:432802/149‑1 (MQ=255)
 ccAGTGCGAAAAGCTGGGCATCGAGCTCACCAACTGTTATGAACACAGCGGCTTACCAGACATTAAAAACGTCACCGGTATTTTAATTGTCGGCAAACCACCGGCCTACTTGCGCGCCGCTGCCAGCGCGTTGACCGACAAGATCTGtt                                                                                                                                            >  1:227647/1‑149 (MQ=255)
            aGCTGGGCATCGAGCTCACCAACTGTTATGAACACAGCGGCTTACCAGACATTAAAAACGTCACCGGTATTTTAATTGTCGGCAGACCCACGCCCGCCCTGCGCGCCGCTGCCAACGCGTTGACCGACAATATCTGTTTTATCGACttt                                                                                                                                 >  2:416054/1‑149 (MQ=255)
                             aCCAACTGTTATGAACACAGCGGCTTACCAGACATTAAAAACGTCACCGGTATTTTAATTGTCGGCAAACCCACGCCCGCCCTGCGCGCCGCTGCCAGCGCGTTGACCGACAATATCTGTTTTATAGACTTTCACGAACCCGGCAGCgg                                                                                                                >  1:321532/1‑149 (MQ=255)
                               cAACTGTTATGAACACAGCGGCTTACCAGACATTAAAAACGTCACCGGTATTTTAATTGTCGGCAAACCCACGCCCGCCCTGCGCGCCGCTGCCAGCGCGTTGACCGACAATATCTGTTTTATCGACTTTCACGAACCCGGCAGCGGtt                                                                                                              >  2:350252/1‑149 (MQ=255)
                                aaCGGTTATGAACACAGCGGCTTATCAGACATTAAAAACGTCACCGGTATTTTAATTGTCGGCAAACCCACGCCCGCCCTGCGCGCCGCTGCCAGCGCGTTGACCGACAATATCTGTTTTATCGACTTTCACGAACCCGGCAGCGGTTa                                                                                                             <  2:170502/149‑1 (MQ=255)
                                       aTGAACACAGCGGCTTACCAGACATTAAAAACGTCACCGGTATTTTAATTGTCGGCAAACCCACGCCCGCCCTGCGCGCAGCTGCCAGCGCGTTGACCGACAATATCTGTTTTATCGACTTTCACGAACCCGGCAGCGGTTACGATGCg                                                                                                      >  2:56510/1‑149 (MQ=255)
                                                  ggCTTACCAGACATTAAAAACGTCACCGGTATTTTAATTGTCGGCAAACCCACGCCCGCCCTGCGCGCCGCTGCCAGCGCGTTGACCGACAATATCTGTTTTATCGACTTTCACGAACCCGGCAGCGGGTACGCTGCGGTGGCTATCGa                                                                                           >  2:359998/1‑149 (MQ=255)
                                                    cTTACCAGACATTAACAACGTCACCGGTCTTTTAATTGTCGGCAACCCCACGCCCGCCCTGCGCGCCGCTGCCAGCGCGTCGAACGATCGTATCTGTTTTATCGACTTTCACGAACCCGGCAGCGGTTACGATGCGGTGGATATCGATc                                                                                         <  1:305442/149‑1 (MQ=255)
                                                     ttACCAGACATTAAAATCGTCACCGGTATTTTAATTGTCGGCAAACCCACGCCCGCCCTGCGCGCCGCTGCCAGCGCGTTGACCGACAATATCTGTTTTATCGACTTTCACGAACCCGGCAGCGGTTACGATGCGGTGGATATCGATCt                                                                                        <  2:227647/149‑1 (MQ=255)
                                                           gACATTAAAAACGTCACCGGTATTTTAATTGTCGGCAAACCCACGCCCGCCCTGCGCGCCGCTGCCAGCGCGTTGACCGACAATATCTGTTTTATCGACTTTCACGAACCCGGCAGCGGTTACGATGCGGTGCATATCGATCTGGCACg                                                                                  >  2:333359/1‑149 (MQ=255)
                                                                tAAAAACGTCACCGGTATTTTAATTGTCGGCAAACCCACGCCCGCCCTGCGCGCCGCTGCCAGCGCGTTGACCGACAATATCTGTTTTATCGACTTTCACGAACCCGGCAGCGGTTACGATGCGGTGGATATCGATCTGGCACGCATCa                                                                             <  2:8409/149‑1 (MQ=255)
                                                                  aaaaCCTCACCGGTATTTTAATTGTCGGCAAACCCACGCCCGCCCTGCGCGCCCCTGCCAGCGCGTTGACCGACAATATCTGTTTTATCGACTTTCACGAACCCGGCAGCGGTTACGATGCGGTGGATATCGATCTGGCACGCATCAGt                                                                           <  1:145568/149‑1 (MQ=255)
                                                                     aCGTCCCCGGTATGTTAATTGTCGGCAAACCCACGCCCGCCCTGCGCGCCGCTGCCAGCGCGTTGACCGACAATATCTGTTTTATCGACTTTCACGAACCCGGCAGCGGTTACGATGCGGTGGATATCGATCTGGCACGCATCAGTaaa                                                                        <  2:48464/149‑1 (MQ=255)
                                                                     aCGTCACCGGTATTTTAATTGTCGGCAAACCCACGCCCGCCCTGCGCGCCGCTGCCAGCGCGTTGACCGACAATATCTGTTTTATCGACTTTCACGAACCTGGCAGCGGTTACGATGCGGTGGATATCGATCTGGCACGCATCAGTaaa                                                                        <  2:333062/149‑1 (MQ=255)
                                                                                aTTTTAATTGTCGGCAAACCCACGCCCGCCCTGCGCGCCGCTGCCAGCGCGTTGACCGACAATATCTGTTTTATCGACTTTCACGAACCCGGCAGCGGTTACGATGCGGTGGATATCGATCTGGCACGCATCAGTAAAGAAATCATCGa                                                             <  2:228704/149‑1 (MQ=255)
                                                                                     agcgggcGGCAAACCCACGCCCGCCCTGCGCGCCGCTGCCAGCGCGTTGACCGACAATATCTGTTTTATCGACTTTCACGAACCCGGCAGCGGTCACGATGCGGTGGATATCGATCTGGCACGCATCAGTAAAGAAATCATCGACTTCt                                                        <  2:515039/143‑1 (MQ=255)
                                                                                      aTTGTCGGCAAACCCACGCCCGCCCTGCGCGCCGCTGCCAGCGCGTTGACCGACAATATCTGTTTTATCGACTTTCa                                                                                                                               >  1:168913/1‑77 (MQ=255)
                                                                                      aTTGTCGGCAAACCCACGCCCGCCCTGCGCGCCGCTGCCAGCGCGTTGACCGACAATATCTGTTTTATCGACTTTCa                                                                                                                               <  2:168913/77‑1 (MQ=255)
                                                                                       ttGTCGGCAAACCCACGCCCGCCCGGCGCGCCGCTGCCAGCGCGTTGACCGACAATATCTGTTTTATCGACTTTCACGAACCCGGCAGCGGTTACGATGCGGTGGCTATCGATCTGGCACGCATCAGTAAAGAAATCATAGACTCCtat                                                      >  2:125577/1‑149 (MQ=255)
                                                                                          gcGGCAAACCCACGCCCGCCCTGCGCGCCGCTGCCAGCGCGTTGACCGACAATATCTGTTTTATCGACTTTCACGAACCCGGCAGCGGTTACGATGCGGTGGATATCGATCTGGCACGCATCAGTAAAGAAATCATCGACTTCTATATc                                                   <  1:375843/148‑1 (MQ=255)
                                                                                             gCAAACCCACGCCCGCCCTGCGCGCCGCTGCCAGCGCGTTGACCGACAATATCTGTTTTATCGACTTTCACGAACCCGGCAGCGGTTACGATGCGGTgg                                                                                                  >  2:7389/1‑99 (MQ=255)
                                                                                             gCAAACCCACGCCCGCCCTGCGCGCCGCTGCCAGCGCGTTGACCGACAATATCTGTTTTATCGACTTTCACGAACCCGGCAGCGGTTACGATGCGGTgg                                                                                                  <  1:7389/99‑1 (MQ=255)
                                                                                               aaaCCCACGCCCGCCCTGCGCGCCGCTGCCAGCGCGTTGACCGACAATATCTGTTTTATCGACTTTCCCGAACCCGGCAGCGGTTACGATGCGGTGGATATCGATCTGGCACGCATCAGTAAAGAAATCATCGGCTTCTATATCACCCa                                              >  1:131515/1‑149 (MQ=255)
                                                                                                  cccACGCCCGCCCTGCGCGCCGCTGCCAGCGCGTTGACCGACAATATCTGTTTTATCGACTTTCACGAACCCGGCATCGGTTACGATGCGGTGGATATCGATCTGGCACGCATCAGTAAAGAAATCATCGACTTCTATATCAACCAggg                                           <  1:458294/149‑1 (MQ=255)
                                                                                                   ccACGCCCGCCCGGCGCGTCGGTGCCAGCGCGTTGACCGACAATATCTGTTTTATCGACTTTCACGAACCCGGCAGCGGTTACGATGCGGTGGATATCGATCTGGCACGCATCAGTAAAGAAATCATCGACTTCTATATCAACCAGGGc                                          <  2:11325/149‑1 (MQ=255)
                                                                                                     aCGCCCGCCCTGCGCGCCGCTGCCAGCGCGTTGACCGACAATATCTGTTTTATCGACTTTCACGAACCCGGCAGCGGTTACGATGCGGTGGATATCGATCTGGCACGCATCAGTAAAGAAATCATCGACTTCTATATCAACCAGGGCGt                                        >  1:481048/1‑149 (MQ=255)
                                                                                                                    gccgcTGCCAGCGCGTTGACCGACAATATCTGTTTTATCGACTTTCACGAACCCGGCAGCGGTTACGATGCGGTGGATATCGATCTGGCACGCATCAGTAAAGAAATCATCGACTTCTATATCAACCAGGGCGTTAATCGTATTGGttt                         <  1:189084/149‑1 (MQ=255)
                                                                                                                       gcTGCCAGCGCGTTGACCGACAATATCTGTTTTATCGACTTTCACGAACCCGGCAGCGGTTACGATGCGGTGGATATCGATCTGGCACGCATCAGTAAAGAAATCATCGACTTCTATATCAACCAGGGCGTTAATCGTATTGGTTTTAt                      <  2:211515/149‑1 (MQ=255)
                                                                                                                        cTGCCAGCGCGTTGACCGACAATATCTGTTTTATCGACTTTCACGAACCCGGCAGCGGTTACGATGCGGTGGATATCGATCTGGCACGCATCAGTAAAGAAATCATCGACTTCTATATCAACCAGGGCGTTAATCGTATTGGTTTTAtt                     <  1:182112/149‑1 (MQ=255)
                                                                                                                            cAGCGCGTTGACCGACAATATCTGTTTTCAAAAATACCGGTAACGCGGCAGCGGTTACGATGCGGTGGATATCGATCTGGCACGCATCAGTAAAGAAATCATCGACTTCTATATCAACCAGGGCGTTAATCGTATTGGTTTTATTGGCg                 >  1:125308/1‑149 (MQ=255)
                                                                                                                                  gTTGGCCGACAATATCTGCATTATCCACTTTCACGAACCCGGCAGCGGTTACGATGCGGTGGATATCGATCTGGCACGCATCAGTAAAGAAATCATCGACTTCTATATCAACCAGGGCGTTAATCGTATTGGTTTTATTGGCGGTGAAg           >  2:500780/1‑149 (MQ=255)
                                                                                                                                   tttaCCGACCATATCTGTTGTAGCGACTTTCACGAACCCGGCAGCGGTTACGATGCGGTGGATATCGATCTGGCACGCATCAGTAAAGAAATCATCGACTTCTATATCAACCAGGGCGTTAATCGTATTGGTTTTATTGGCGGTGAAGa          <  1:422127/146‑1 (MQ=255)
                                                                                                                                    tGACCGACAATATCTGTTTTATCGACTTTCACGAACCCGGCAGCGGTTACGATGCGGTGGATATCGATCTGGCACGCATCAGTAAAGAAATCATCGACTTCTATATCAACCAGGGCGTTAATCGTATTGGTTTTATTGGCGGTGAAGAt         <  1:350252/149‑1 (MQ=255)
                                                                                                                                        cGACAATATCTGTTTTATCGACTTTCACGAACCCGGCAGCGGTTACGATGCGGTGGATATCGATCTGGCACGCATCAGTAAAGAAATCATCGACTTCTATATCAACCAGGGCGTTAATCGTATTGGTTTTATTGGCGGTGAAGATGAGc     <  1:56510/149‑1 (MQ=255)
                                                                                                                                           cAATATCTGTTTTATCGACTTTCACGAACCCGGCAGCGGTTACGATGCGGTGGATATCGATCTGGCACGCATCAGTAAAGAAATCATCGACTTCTACATCAACCAGGGCGTTAATCGTATTGGTTTTATTGGCGGTGAAGATGAGCCTg  <  1:416054/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                
CCCAGTGCGAAAAGCTGGGCATCGAGCTCACCAACTGTTATGAACACAGCGGCTTACCAGACATTAAAAACGTCACCGGTATTTTAATTGTCGGCAAACCCACGCCCGCCCTGCGCGCCGCTGCCAGCGCGTTGACCGACAATATCTGTTTTATCGACTTTCACGAACCCGGCAGCGGTTACGATGCGGTGGATATCGATCTGGCACGCATCAGTAAAGAAATCATCGACTTCTATATCAACCAGGGCGTTAATCGTATTGGTTTTATTGGCGGTGAAGATGAGCCTG  >  NC_000913_3_pae_pgi/3221737‑3222024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.